Navigation Links
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

roximately four times faster on His GraviTrap (Fig 2B).

Complete details of these comparisons are provided in data file 11-0036-90, available at www.amershambiosciences.com/promo_dm205

His GraviTrap also enables rapid separation of large proteins. To demonstrate this, His GraviTrap was used to purify (His)10-TRX-P450 (Mr 133 200). E. coli JM109 cells expressing (His)10-TRX-P450 were enzymatically and mechanically lysed and a 20 ml clarified sample was loaded onto His GraviTrap using LabMate. The whole purification took just 25 min.

Results show that the purification yielded three major protein bands when analyzed by SDS-PAGE (Fig 3A, lane 4). Western blot analysis confirms that each of the three bands contains a histidine-tag (Fig 3B). However, only a weak band can be seen from the band at approximately Mr 15 000. Some of the smaller proteins may have passed through the membrane due to a prolonged blotting time. N-terminal sequencing (data not shown) of the three bands confirms that the low molecular weight bands are truncated forms of the histidine-tagged target protein. To separate the full-length protein from the truncated forms, a second purification step, such as gel filtration, is recommended.


Summary
His GraviTrap columns provide a very fast and simple method for purifying histidine-tagged proteins without the need for a pump or purification system. The prepacked Ni Sepharose 6 Fast Flow gives high protein binding capacity. Proven protocols, application data, and new support products help optimize purification results and simplify the whole procedure.

To obtain data file 11-0036-90, visit www.am
'"/>


Source:


Page: All 1 2 3 4

Related biology technology :

1. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
2. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
3. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
4. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
5. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
6. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
7. Walkaway automation for nucleic acid purification
8. DNA purification from pig ears to bird blood using proven DNeasy Technology
9. Addition of imidazole during binding improves purity of histidine-tagged proteins
10. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
11. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
Post Your Comments:
(Date:8/21/2014)... August 21, 2014 His Majesty Willem-Alexander, ... , The Court of His Majesty Willem-Alexander, King of ... the Grand Opening of POET-DSM Advanced Biofuels’ Project LIBERTY ... 3. , The King will take part in the ... scheduled for 11 a.m.-12:20 p.m. and tour the plant. ...
(Date:8/21/2014)... , Aug. 21, 2014  Decision Resources Group ... Brazil and Mexico ... of several newly approved agents, the anticipated label ... and the launch of emerging biologics will substantially ... particular, the emerging IL-5 inhibitors will introduce a ...
(Date:8/21/2014)... KONG , Aug. 21, 2014  China Cord Blood ... announced its plan to release financial results for the ... 28, 2014, after market close in the US.  ... at 8:00 a.m. ET on Friday, August 29, 2014 ... overview of the Company,s recent developments, followed by a ...
(Date:8/21/2014)... (PRWEB) August 21, 2014 Ontotext ... today by Ontotext . Now the same ... analysis of structured and unstructured data is available to ... cost of enterprise technology. Organizations that do not have ... data management can use S4 since there is no ...
Breaking Biology Technology:His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 2His Majesty Willem-Alexander, King of the Netherlands, to Attend Project LIBERTY opening 3Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3China Cord Blood Corporation to Report First Quarter of Fiscal 2015 Financial Results 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 3Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 4
... 26, 2011 Reportlinker.com announces that a ... its catalogue: Future Directions ... http://www.reportlinker.com/p0511976/Future-Directions-in-Regenerative-Medicine.html?utm_source=prnewswire&utm_medium=pr&utm_campaign=Biological_Therapy Big Pharma has ... 2008, Pfizer and Novartis in 2009, and ...
... SAN DIEGO, May 26, 2011 Biomatrica, Inc., the ... announced the appointment of Dr. James A. Robb to ... is a well respected pathologist and scientist with over ... virology, and genetics. He currently serves as a Governor ...
... class of decorative, flower-like defects in the nanomaterial graphene ... unique electrical and mechanical properties, according to researchers at ... Georgia Tech. In a new paper,* the team for ... that could occur naturally or be induced to occur ...
Cached Biology Technology:Reportlinker Adds Future Directions in Regenerative Medicine 2Reportlinker Adds Future Directions in Regenerative Medicine 3Reportlinker Adds Future Directions in Regenerative Medicine 4Reportlinker Adds Future Directions in Regenerative Medicine 5Reportlinker Adds Future Directions in Regenerative Medicine 6Biomatrica Announces the Appointment of Dr. James A. Robb as a Member of the Scientific Advisory Board 2Biomatrica Announces the Appointment of Dr. James A. Robb as a Member of the Scientific Advisory Board 3Defect in graphene may present bouquet of possibilities 2
(Date:8/21/2014)... intake, health and quality of life of older adults ... the University of Waterloo receiving close to $1.5 million ... (CIHR). , Professor Heather Keller, of the Faculty ... research chair in nutrition and aging, will receive $979,000 ... in long-term care homes are poorly nourished. The project ...
(Date:8/21/2014)... a finding that has implications for life in other ... the solar system, LSU Associate Professor of Biological Sciences ... Science Foundation, or NSF, this week published a paper ... that lies 800 meters (2600 feet) beneath the surface ... ecosystems.", Given that more than 400 subglacial lakes and ...
(Date:8/21/2014)... Ever heard of the water window? It consists of ... are not absorbed by the water in biological tissues. ... develop coherent radiations within the water window. These could ... a high-contrast image of the biological samples or to ... study identifies the physical mechanism needed to efficiently generate ...
Breaking Biology News(10 mins):Grants will fund landmark aging research at Waterloo 2800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 2800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 3800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 4Water window imaging opportunity 2
... available in German . Flowers need water ... to gather energy from earth and water. Members of Professor Dr. ... groundbreaking discovery that one plant has another way of doing this. ... green alga Chlamydomonas reinhardtii , not only engages in photosynthesis, ...
... WINSTON-SALEM, N.C. Nov. 20, 2012 If you are ... then you have probably tried everything from warm milk ... with varying degrees of success and side effects. But what ... through ,balancing, brain activity? Researchers at Wake Forest ...
... , Sequencing historical DNA to tackle wheat,s worst ... extremes, Leaving a bad taste in aphids, mouths, Reducing ... Exploiting wild wheat to produce better Indian varieties , ... a unique 16M initiative that will harness bioscience to ...
Cached Biology News:Algae can draw energy from other plants 2Music to the ears for a good night's sleep? 2Music to the ears for a good night's sleep? 3Major international push to maximize bioscience research to help world's poorest farmers 2Major international push to maximize bioscience research to help world's poorest farmers 3Major international push to maximize bioscience research to help world's poorest farmers 4
Screening test for the detection of Salmonella sp. by means of DNA amplification, subsequent molecular hybridization and detection on a microtitre plate....
... 10X PCR Enhancer (with betaine) substantially ... sensitivity and specificity for amplification of ... Enhancer in PCR eliminates the base-pair ... enzyme?s thermal stability, suppresses pauses of ...
... be used with all 96-well ... stacking of covered plates ... with automated systems ... are available for use with ...
Designed for use with all 96-well SPRI reagent kits. Used with round bottom 96-well microplates, and 96-well thermal cycling plates....
Biology Products: