Navigation Links
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

roximately four times faster on His GraviTrap (Fig 2B).

Complete details of these comparisons are provided in data file 11-0036-90, available at www.amershambiosciences.com/promo_dm205

His GraviTrap also enables rapid separation of large proteins. To demonstrate this, His GraviTrap was used to purify (His)10-TRX-P450 (Mr 133 200). E. coli JM109 cells expressing (His)10-TRX-P450 were enzymatically and mechanically lysed and a 20 ml clarified sample was loaded onto His GraviTrap using LabMate. The whole purification took just 25 min.

Results show that the purification yielded three major protein bands when analyzed by SDS-PAGE (Fig 3A, lane 4). Western blot analysis confirms that each of the three bands contains a histidine-tag (Fig 3B). However, only a weak band can be seen from the band at approximately Mr 15 000. Some of the smaller proteins may have passed through the membrane due to a prolonged blotting time. N-terminal sequencing (data not shown) of the three bands confirms that the low molecular weight bands are truncated forms of the histidine-tagged target protein. To separate the full-length protein from the truncated forms, a second purification step, such as gel filtration, is recommended.


Summary
His GraviTrap columns provide a very fast and simple method for purifying histidine-tagged proteins without the need for a pump or purification system. The prepacked Ni Sepharose 6 Fast Flow gives high protein binding capacity. Proven protocols, application data, and new support products help optimize purification results and simplify the whole procedure.

To obtain data file 11-0036-90, visit www.am
'"/>


Source:


Page: All 1 2 3 4

Related biology technology :

1. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
2. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
3. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
4. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
5. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
6. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
7. Walkaway automation for nucleic acid purification
8. DNA purification from pig ears to bird blood using proven DNeasy Technology
9. Addition of imidazole during binding improves purity of histidine-tagged proteins
10. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
11. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
Post Your Comments:
(Date:9/30/2014)... . , Electrical ... a new kind of building block for digital integrated ... be based on three-dimensional arrangements of nanometer-scale magnets instead ... semiconductor industry CMOS fabrication of silicon chips ... the University of Notre Dame are exploring "magnetic computing" ...
(Date:9/30/2014)... , Sept. 30, 2014 Report ... segments with highest potential revenues Do you want ... report gives you revenue predictions for those biological ... those therapies you find financial data, R&D trends, ... you get sales forecasts to 2024 at overall ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 Over the ... primarily due to rising R&D budgets and increased acceptance ... fast growing pharmaceutical and biotechnology industries are also catalyzing ... laboratory filtration market is expected to grow at a ... is estimated to be worth $2,532.6 million by 2019. ...
(Date:9/30/2014)... Sept. 30, 2014  RegeneRx Biopharmaceuticals, Inc. (OTC: ... it has received a Canadian Patent for Inhibition ... This patent includes claims using thymosin beta 4, ... peptides for these purposes.  The patent will expire ... Inc. ( www.regenerx.com ) RegeneRx ...
Breaking Biology Technology:A new dimension for integrated circuits: 3-D nanomagnetic logic 2A new dimension for integrated circuits: 3-D nanomagnetic logic 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 2Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 3Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 4Global Laboratory Filtration Market To See 7.0% CAGR to 2019 Says a Research Report Available at ReportsnReports.com 5RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2
... solopack Gold cells reduce the time and effort needed ... Amy Duncan Bruce,Jerpseth Alan Greener ,Stratagene ... , strain* in an easy-to-use, single-reaction format ... 9 transformants/g of,supercoiled DNA or the competent efficiency ...
... purification from a more versatile vector , ... ,Epitope Tagging in E. coli , Katherine Felts ... ,Stratagene , Stratagene has improved the Affinity ... vector. This new E. coli cloning and expression,vector ...
... data with the MutaTrak electronic notebook , G. ... Hamovitch Doug Schroeder ,Solana Consulting Group, Solana Beach, ... record Big,Blue transgenic rodent ... entered in a logical order and updated easily,as an ...
Cached Biology Technology:XL10-Gold Cells Supplied in New Single-Reaction Format 2XL10-Gold Cells Supplied in New Single-Reaction Format 3XL10-Gold Cells Supplied in New Single-Reaction Format 4High-Level Protein Expression, One-Column Purification, and FLAG,Epitope Tagging in E. coli 2High-Level Protein Expression, One-Column Purification, and FLAG,Epitope Tagging in E. coli 3High-Level Protein Expression, One-Column Purification, and FLAG,Epitope Tagging in E. coli 4High-Level Protein Expression, One-Column Purification, and FLAG,Epitope Tagging in E. coli 5High-Level Protein Expression, One-Column Purification, and FLAG,Epitope Tagging in E. coli 6High-Level Protein Expression, One-Column Purification, and FLAG,Epitope Tagging in E. coli 7High-Level Protein Expression, One-Column Purification, and FLAG,Epitope Tagging in E. coli 8Organize Your Data from the Big Blue Mutation Assay 2Organize Your Data from the Big Blue Mutation Assay 3
(Date:9/30/2014)... Fish can live in almost any aquatic environment on ... up many species are pushed to the limit. The ... could prove critical for how different species cope in ... the University of Gothenburg, published in the scientific journal ... Climate change continues apace thanks to increasing levels of ...
(Date:9/30/2014)... in Munich report that a new class of ... chemotherapeutic drugs. They have also pinpointed the relevant ... anti-tumor agents. , Researchers led by LMU,s ... the Technische Universitt Mnchen have identified a class ... in the fight against malignant tumors. The compound ...
(Date:9/30/2014)... Mass. , Sept. 30, 2014 /PRNewswire/ ... control solutions for the biotech and power ... its new RECONN Bioprocess Manager, an easy-to-use ... solution geared towards bioprocess development laboratories. ... have disparate bioreactor and analyzer systems. Accessing ...
Breaking Biology News(10 mins):Shape up quickly -- applies to fish, too! 2Cancer therapy: Driving cancer cells to suicide 2Spectra Automation Introduces RECONN Bioprocess Manager 2
... have marine biologists come to learn about the eye-opening characteristics ... bones of whale carcasses. With each new study, scientists ... Osedax , a genus of mouthless and gutless "bone worms" ... In the latest finding, scientists at Scripps Institution of Oceanography ...
... pilot study suggest that a virtual patient advocate (VPA) ... healthier pregnancies. Developed at Boston University School of Medicine ... is an innovative tool developed to deliver preconception care ... The study results, which are published online ...
... University professorsDavid Heeger, Joseph LeDoux, and Ruth Nussenzweighave been ... D.C.-based organization announced today. Heeger and LeDoux, ... and Department of Psychology, and Nussenzweig, a professor in ... were among the 84 new members and 21 foreign ...
Cached Biology News:Bizarre bone worms emit acid to feast on whale skeletons 2Virtual patient advocate delivers preconception care to improve pregnancy outcomes 2Virtual patient advocate delivers preconception care to improve pregnancy outcomes 33 NYU faculty elected to National Academy of Sciences 2
...
Antibody Diluent for IHC 125 ml...
Sold individually or included in the MasterAmp™ PCR Optimization Kit without ammonium sulfate. Includes dNTPs, buffer, and varying amounts of MgCl2 and the MasterAmp™ PCR Enhancer (with be...
QPRT Antibody...
Biology Products: