Navigation Links
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

e used to collect liquid waste.

The optional LabMate reservoir increases the loading capacity from 10 ml to approximately 35 ml. Large volumes can thus be applied in one go without having to constantly attend the column to add more sample.

The new His Buffer Kit delivers added convenience by providing phosphate buffer concentrates and highly pure 2 M imidazole stock solutions. This kit eliminates time-consuming manual buffer preparation, thereby promoting optimal convenience, performance, and reproducibility.

Faster purifications under a wide range of conditions
The performance of His GraviTrap was compared with Ni-NTA Superflow™ gravity-flow column (Qiagen Inc) during the purification of histidine-tagged green fluorescent protein (GFP-[His]6; Mr 28 000) from an E. coli lysate. The imidazole concentration in the sample and binding buffers was 45 mM for His GraviTrap and 10 mM for Ni-NTA Superflow (the latter according to manufacturer’s recommendations).

The recovery was greater than 98% in the first 3 ml eluate from His GraviTrap, and approximately 80% from the other column. For complete elution from Ni-NTA Superflow, a total volume of 6 to 9 ml was needed, and the total purification time was close to 100 min. The total purification time was 20 min when His GraviTrap was used (Fig 2A). The purity of the eluates was similar as determined by SDS-PAGE (data not shown).

His GraviTrap and Ni-NTA Superflow columns were also compared under denaturing conditions. Histidine-tagged Maltose Binding Protein (MBP-(His)6; Mr 43 000) in E. coli BL-21 lysate containing 7 M urea was applied to the columns. Elution was achieved by lowering the pH from pH 8.0 to pH 4.5. Although denaturing conditions increase the purification time due to the higher viscosity of the samples and buffers, total purification time was still app
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
2. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
3. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
4. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
5. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
6. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
7. Walkaway automation for nucleic acid purification
8. DNA purification from pig ears to bird blood using proven DNeasy Technology
9. Addition of imidazole during binding improves purity of histidine-tagged proteins
10. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
11. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
Post Your Comments:
(Date:9/19/2014)... rarely simple. From crop yields to disease risks, the ... considered "complex traits." Just as for heightthe textbook example ... human disease are shaped by multiple genetic and environmental ... To track down such genes, geneticists typically mate two ... mouse and a small mouseand then study their descendents, ...
(Date:9/19/2014)... 2014 Follow us on ... revolutionize the world has attracted diverse players including ... start-ups. The early years of nanotechnology development was ... to the high risks involved, which deterred private ... this evolving technology have been growing over the ...
(Date:9/19/2014)... Sept. 19, 2014  Nektar Therapeutics (NASDAQ: ... studies characterizing the analgesic profiles of a series ... receptor agonist molecules. The preclinical research candidates were ... platform. The analgesic properties of kappa ... literature. 1,2 Kappa opioid receptors are expressed ...
(Date:9/19/2014)... 2014 Barnhardt Manufacturing Company ... its Organic Cotton . The USDA Certified Biobased ... biobased ingredients meets or exceeds levels set by USDA. ... whole or in significant part of agricultural, forestry, or ... Organic cotton to be immediately identified as a USDA ...
Breaking Biology Technology:Want to link genes to complex traits? Start with more diversity 2Growing R&D Investments & Developments in Nanomaterials to Drive the Global Nanotechnology Market, According to New Report by Global Industry Analysts, Inc. 2Growing R&D Investments & Developments in Nanomaterials to Drive the Global Nanotechnology Market, According to New Report by Global Industry Analysts, Inc. 3Growing R&D Investments & Developments in Nanomaterials to Drive the Global Nanotechnology Market, According to New Report by Global Industry Analysts, Inc. 4Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 2Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 4Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 5Barnhardt Manufacturing Company Earns USDA Certified Biobased Product Certification and Label for Organic Cotton 2
... WILMINGTON, Del., Sept. 25 The results ... Proof Of Concept) Study of ZD4054 -- a ... today at the 14th European Congress,of Clinical Oncology ... ZD4054, an investigational compound in development for the,treatment ...
... MDVN ) today announced that it has been ... new NASDAQ(R) NeuroInsights(R) Neurotech Index.,The Index launched today and ... a total return basis (Nasdaq: NRVX.) "Medivation,s selection ... industry is testament to our commitment to and significant,clinical ...
... FRANCISCO, Sept. 25 BioSpace, the leading online,source ... the 2008 BIO,International Convention and BIOCOM, unveiled this ... Campaign. The campaign features both a,regional promotional Hotbed ... with the economic and workforce development initiatives of,Southern ...
Cached Biology Technology:Survival Data Presented From Phase II Study of Investigational Drug ZD4054 in Hormone-Resistant Prostate Cancer Patients 2Survival Data Presented From Phase II Study of Investigational Drug ZD4054 in Hormone-Resistant Prostate Cancer Patients 3Survival Data Presented From Phase II Study of Investigational Drug ZD4054 in Hormone-Resistant Prostate Cancer Patients 4Medivation Selected as Founding Member of New NASDAQ(R) NeuroInsights(R) Neurotech Index 2Medivation Selected as Founding Member of New NASDAQ(R) NeuroInsights(R) Neurotech Index 3BioSpace, 2008 BIO International Convention and BIOCOM Launch 9th Edition of Biotech Beach Hotbed Map Campaign 2
(Date:9/21/2014)... newborn immune T cells may have the ability to ... new study led by King,s College London. Although their ... babies may still be able to mount a strong ... Nature Medicine . , Our immune system is ... including neutrophils which play an important role in the ...
(Date:9/19/2014)... will study the unique bioelectric signaling system of the ... health. The OU researcher is working to understand ... the signals used to map the world around them. ... of 500-600 discharges a second throughout their lives. ... extreme, but necessary for survival. , "There is evidence ...
(Date:9/19/2014)... legume plants regulate their symbiotic relationship with soil ... through the plant structure from leaves into the ... in the roots. This collaborative study was conducted ... Biology, the Graduate University for Advanced Studies (SOKENDAI), ... in Japan. , Legumes, an important plant ...
Breaking Biology News(10 mins):Immune system of newborn babies is stronger than previously thought 2For legume plants, a new route from shoot to root 2
... seeps are deep-sea environments, usually a few square meters ... from the sea floor. Mud volcanoes which are active ... in the 1990s. These harsh conditions give rise to ... for life to exist on the planet. , ...
... protein crucial for the growth of brain cells during ... of long-term memories, according to researchers at UC Irvine. ... and treatments for, cognitive decline associated with normal aging ... appear in the early online edition of the Proceedings ...
... turning on a single gene, researchers can prevent skin stem ... cells -- epidermal, sebaceous and hair cells. They say this ... stem cells in the lab, for both research and potential ... which have the ability to differentiate into multiple types of ...
Cached Biology News:Life in the extreme 2Proteins necessary for brain development found to be critical for long-term memory 2Researchers identify a key regulator for skin stem cells 2
Carrier Serum...
A Highly Purified, Cost-Effective Taq DNA Polymerase...
Homo sapiens similar to Cytosolic acyl coenzyme A thioester hydrolase Antigen: Peptide...
... is a protein based inhibitor of nonhuman ... most common and troublesome RNases including RNase ... can be used in any application where ... ideal for in vitro transcription and translation, ...
Biology Products: