Navigation Links
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

e used to collect liquid waste.

The optional LabMate reservoir increases the loading capacity from 10 ml to approximately 35 ml. Large volumes can thus be applied in one go without having to constantly attend the column to add more sample.

The new His Buffer Kit delivers added convenience by providing phosphate buffer concentrates and highly pure 2 M imidazole stock solutions. This kit eliminates time-consuming manual buffer preparation, thereby promoting optimal convenience, performance, and reproducibility.

Faster purifications under a wide range of conditions
The performance of His GraviTrap was compared with Ni-NTA Superflow™ gravity-flow column (Qiagen Inc) during the purification of histidine-tagged green fluorescent protein (GFP-[His]6; Mr 28 000) from an E. coli lysate. The imidazole concentration in the sample and binding buffers was 45 mM for His GraviTrap and 10 mM for Ni-NTA Superflow (the latter according to manufacturer’s recommendations).

The recovery was greater than 98% in the first 3 ml eluate from His GraviTrap, and approximately 80% from the other column. For complete elution from Ni-NTA Superflow, a total volume of 6 to 9 ml was needed, and the total purification time was close to 100 min. The total purification time was 20 min when His GraviTrap was used (Fig 2A). The purity of the eluates was similar as determined by SDS-PAGE (data not shown).

His GraviTrap and Ni-NTA Superflow columns were also compared under denaturing conditions. Histidine-tagged Maltose Binding Protein (MBP-(His)6; Mr 43 000) in E. coli BL-21 lysate containing 7 M urea was applied to the columns. Elution was achieved by lowering the pH from pH 8.0 to pH 4.5. Although denaturing conditions increase the purification time due to the higher viscosity of the samples and buffers, total purification time was still app
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
2. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
3. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
4. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
5. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
6. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
7. Walkaway automation for nucleic acid purification
8. DNA purification from pig ears to bird blood using proven DNeasy Technology
9. Addition of imidazole during binding improves purity of histidine-tagged proteins
10. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
11. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
Post Your Comments:
(Date:7/30/2014)... Md. , July 30, 2014 ... components to interrogate biology, but such devices can ... As such, researchers have looked ... in vitro metabolic engineering to characterize ... a chip. New devices are envisioned to recreate ...
(Date:7/30/2014)... July 30, 2014  RXi Pharmaceuticals Corporation ... company focused on discovering, developing and commercializing ... using RNA-targeted technologies, today announced that their ... for the reduction of recurrence of hypertrophic ... been initiated. Logo - ...
(Date:7/30/2014)... 2014 Vala Sciences was recently ... Protection Agency (EPA). This new contract was awarded ... including tests designed to assess the effect of ... of connections between neurons (synapses), which are critical ... formation by environmental pollutants likely contributes to a ...
(Date:7/30/2014)... July 30, 2014 LayerBio, Inc., an ... of its first round of seed financing from friends ... preliminary efforts to develop a novel sustained-release product for ... our investors and their commitment to this important work," ... "Ophthalmology is one of many areas in which innovations ...
Breaking Biology Technology:UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 2RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 3RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 4RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 5U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 2U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 3
... NEW YORK, April 4, 2011 Reportlinker.com ... is available in its catalogue: ... companies http://www.reportlinker.com/p0203535/Antiviral-Therapeutics---Technologies-markets-and-companies.html ... current state-of-art of antiviral approaches including vaccines, ...
... NEW YORK, April 4, 2011 Scientists at Polytechnic ... NYU College of Dentistry have discovered a biochemical version ... the "peanut butter and chocolate" rule:  Sometimes two things ... https://photos.prnewswire.com/prnh/20091027/NY99197LOGO ) Seiichi Yamano , ...
... 4, 2011 CryoLife, Inc. (NYSE: CRY ), ... announced today that it has filed an IDE ... begin clinical trials for the purpose of obtaining Pre-Market Approval ... during surgical procedures or following traumatic injuries. ...
Cached Biology Technology:Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 2Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 3Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 4New Research Holds Promise for Gene Engineering and Therapy 2New Research Holds Promise for Gene Engineering and Therapy 3New Research Holds Promise for Gene Engineering and Therapy 4CryoLife Files Investigational Device Exemption (IDE) to Begin Clinical Trials for PerClot® in the U.S. 2CryoLife Files Investigational Device Exemption (IDE) to Begin Clinical Trials for PerClot® in the U.S. 3CryoLife Files Investigational Device Exemption (IDE) to Begin Clinical Trials for PerClot® in the U.S. 4
(Date:7/30/2014)... change on the world,s animal and plant species ... a new paper by a research team from ... other organizations. The authors claim that most of ... it comes to climate change. , While ... the "direct" threats of changing temperatures and precipitation ...
(Date:7/30/2014)... Press (OUP) is pleased to announce its new relationship ... 2015, OUP and CINP will partner to publish ... a fully open-access journal. , Alan Frazer, Editor-in-Chief of ... size and expertise of OUP will further facilitate the ... of the very leading journals within neuropsychopharmacology. The conversion ...
(Date:7/30/2014)... July 30, 2014 ResearchMoz ... Government Biometric Systems Market 2014-2024   Business Strategies, Industry ... Biometric Systems Market 2014-2024" offers the reader detailed ... the next ten years, alongside potential market opportunities ... forecasts. To view the table of ...
Breaking Biology News(10 mins):Conservation scientists asking wrong questions on climate change impacts on wildlife 2OUP to partner with the International College of Neuropsychopharmacology 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 2Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 3Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 4Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 5Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 6Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 7Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 8Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 9Global Government Biometric Systems Market Size, Share, Trends and Forecasts 2014-2024 Available Online by Researchmoz.us 10
... , This release is available in French . ... affects over 12 million people worldwide, with more than 2 million ... how the parasite survives inside human cells. That mystery has now ... Signaling by a team led by Dr. Martin Olivier ...
... of liquorice during pregnancy could adversely affect their child,s ... study of eight year old children whose mothers ate ... not perform as well as other youngsters in cognitive ... poor attention spans and show disruptive behaviour such as ...
... Vaccination can lower children,s risk of allergy. Cathleen ... for allergy prevention in the current issue of ... 625-31). Allergic diseases are becoming increasingly common in ... based treatment of allergic asthma, hay fever, or atopic ...
Cached Biology News:Major discovery opens door to leishmania treatment 2Eating liquorice in pregnancy may affect a child's IQ and behavior 2
... trays are disposable trays that ... that have been blotted onto ... eight 10.5 cm x 5 ... cut from blotted membrane and ...
... The ProteoPrep 20 Plasma Immunodepletion Kit specifically ... from human plasma or serum in preparation ... deeper penetration into the plasma proteome whether ... or multidimensional chromatography or go straight to ...
... introduced the industry's first siRNA sequence design ... to keep these tools consistent with new ... the field of RNAi. With the ... RNAi design tools, OligoEngine is pleased to ...
... Signal Enhancer does for enzyme-/substrate-based blotting what intensifying ... the signal up to 10-fold (or one order ... Western Blot Signal Enhancer membrane treatment is a ... to your current Western blotting protocol. The result ...
Biology Products: