Navigation Links
New Yeast Cloning System for Producing Proteins with Native Amino Acid,,,Sequences

One-column protein purification and native eukaryotic amino acid sequence

Quinn Lu John Bauer
Stratagene Cloning Systems, Inc.

The ESP LIC cloning and expression system features rapid and high-yield production of recombinant proteins in yeast. This system uses ligation-independent cloning (LIC) for efficient and directional cloning of PCR products immediately downstream of the glutathione-S-transferase (GST) purification tag and the recognition site for enterokinase. After expression and single-column purification of the GST fusion protein, the purification tag can be removed with enterokinase, which cleaves C-terminal to its recognition site, producing polypeptides with completely native amino acid sequences.

figure 1

Stratagenes ESP LIC cloning and expression system offers rapid and high-yield production of recombinant proteins in a eukaryotic organism. Because the system uses the yeast Schizosaccharomyces pombe as the expression host, proteins undergo many of the posttranslational modifications that are often required for their biological activity. This system also features the new pESP-2 vector (figure 1), which retains all of the unique properties of the pESP-1 vector,1 such as tight transcriptional regulation by thiamine and high expression levels. The distinct advantage that the pESP-2 vector offers is efficient and directional LIC2-4 cloning of PCR products immediately downstream of a GST purification tag.

Cloning PCR products using the LIC strategy is so efficient that as little as 20 ng of PCR product is required for each cloning experiment. PCR-generated sequence errors are minimized by using fewer amplification cycles (e.g., 15 cycl
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. Yeast Protein Production System Features High Yields and One-Step Purification
3. Electroporation of Yeast Artificial Chromosomes
4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
5. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
6. Enhanced PCR Cloning System
7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
8. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
9. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
10. QIAGEN PCR CloningPlus Kit
11. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
Post Your Comments:
(Date:7/28/2014)... 28, 2014   Propeller Health , the FDA-cleared ... hire of Brad Towle as Senior Vice ... for driving the company,s sales growth, customer acquisition and ... and specifically his past role in the development of ... at this pivotal time in the industry," said ...
(Date:7/28/2014)... , July 28, 2014  InterMune, Inc. (NASDAQ: ... release its second quarter 2014 financial results at the ... 2014. A live conference call and webcast will be hosted ... day. Interested investors and others may participate ... +1 212-231-2900 (international), conference ID#21728775. A replay of the webcast ...
(Date:7/26/2014)... Canada Endoscopy Visualization Systems Market Outlook ... Canada Endoscopy Visualization Systems market. The report provides ... units) and average prices (in US dollars) within ... Endoscopy Visualization Systems and Low-End Endoscopy Visualization Systems. ... , The report also provides company shares and ...
(Date:7/25/2014)... by researchers from the University of Leicester has ... unlocking the potential to create new materials using ... been published in the prestigious academic journal ... technique to examine in rich detail the structure ... containing an acetylene molecule and a single helium ...
Breaking Biology Technology:Propeller Health Hires Brad Towle as Senior Vice President of Business and Client Development 2InterMune to Release Second Quarter Financial Results on August 6 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 3Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 5Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 2Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster 3
... announces that a new market research report is ... Adds Global Top 10 Biotechnology Companies -- Industry, ... http://www.reportlinker.com/p0155124/Reportlinker-Adds-Global-Top-10-Biotechnology-Companies----Industry-Financial-and-SWOT-Analysis.html ... Companies Report: Strategic evaluation of industry and key ...
... October 16 , - New ... Brain Science and Used by the National Institutes of,Health, Will ... Elsevier, the leading publisher of scientific, ... showcase the new,features it is rolling out for its BrainNavigator ...
... Texas A team of scientists, led by a biomedical ... for the first time that mathematical models created ... correctly predict previously unknown cellular mechanisms. This brings biologists a ... control the inner workings of the cell as readily as ...
Cached Biology Technology:Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 2Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 3Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 4Reportlinker Adds Global Top 10 Biotechnology Companies -- Industry, Financial and SWOT Analysis 5Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 2Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 3Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 4Elsevier's BrainNavigator Research Tool to Launch new Features at Neuroscience 2009 Show 5Scientists use math modeling to predict unknown biological mechanism of regulation 2
(Date:7/27/2014)... new in vitro study has revealed that nicotine and ... damage caused by a certain carcinogen in smoke. , ... curing of tobacco leaves and ultimately ends up in ... the lung and liver, where it is activated by ... research in mice has revealed that nicotine can partially ...
(Date:7/27/2014)... have discovered a new species of mayfly in the southern ... India. In fact, this is the first time that any ... collected in peninsular India. , The new species, called ... Soldan for his substantial contribution to the understanding of the ... of a study that describes the new mayfly in the ...
(Date:7/27/2014)... at Barts Cancer Institute have found that targeting a ... more effective, according to research published in Nature ... part of Queen Mary University of London, have found ... the body to repair itself after chemotherapy or radiotherapy, ... researchers removed FAK from blood vessels that grew in ...
Breaking Biology News(10 mins):Nicotine found to inhibit DNA-strand break caused by a certain carcinogen in smoke 2New drug target can break down cancer's barrier against treatment 2
... the UN Conference on Climate Change (COP15) to be held ... to organise this conference as part of the run-up to ... try and capture some of the enormous research energy currently ... change. Thus, the focus of the conference is on providing ...
... of Southern California School of Dentistry researchers says it ... some people taking drugs that treat osteoporosis. Microbial ... are causing jaw tissue infections in patients taking bisphosphonate ... professor at the USC School of Dentistry. ...
... Stem cell researchers at UCLA were able to grow functioning ... into cells with the same unlimited properties as embryonic stem ... that induced pluripotent stem cells or iPS cells, which dont ... into the three types of cardiovascular cells needed to repair ...
Cached Biology News:USC School of Dentistry researchers uncover link between osteoporosis drugs and jaw infection 2UCLA stem cell researchers create heart and blood cells from reprogrammed skin cells 2UCLA stem cell researchers create heart and blood cells from reprogrammed skin cells 3
... Bruker Daltonics and Bruker BioSpins ... for metabolism studies, and analysis of ... ESI-TOF system, an optional Avance NMR ... acquisition, evaluation and statistical analysis for ...
... animal movements in a Morris Water Maze. ... a predefined sequence of trials.The Spatial Behavior line ... a video camera, frame grabber, and feature extraction ... once and up to 6 moving animals. ...
... Object Records behavior directed toward examining a novel ... a family of instruments all built around a ... and that can track two fields at once ... automatically extracts animal positions and records them, but ...
... Ultrapure ACS Reagent Grade C 19 H ... Weight: 691.95 PRODUCT SPECIFICATIONS Form: Powder Clarity ... pH 3.0 - Yellow pH ... tracking dye in agarose and acrylamide gel ...
Biology Products: