Navigation Links
New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes


High-level expression of heterologous proteins

Carsten-Peter Carstens Julie Bonnardel Anna Waesche Ronda Allen
Stratagene

Codon bias is a significant obstacle for efficient expression of heterologous genes in E. coli hosts. In GC-rich genomes, such as mammals, rare arginine codons (AGG or AGA) and the proline codon (CCC) most frequently affect bacterial gene expression. For such situations, Stratagene introduces BL21-CodonPlus-RP cells* to make protein expression more reliable. This strain contains a ColE1-compatible plasmid that encodes extra copies of the argU and proL tRNA genes and is able to rescue expression of genes restricted by either AGG/ AGA codons or CCC codons.

The E. coli BL21 strains offer many advantages for expression of heterologous proteins. Derived from E. coli B, these strains naturally lack the Lon protease and are engineered to be deficient for the OmpT protease. The BL21(DE3) and the BL21(DE3)pLysS hosts permit induced expression of T7 RNA polymerase. When used in conjunction with pET-type vectors, the expressed T7 RNA polymerase provides significant levels of transcription of heterologous genes. Stratagenes BL21-Gold competent cells** are another member of the BL21 family of cells that feature two additional advantages: They are EndA1 deficient, which allows preparation of high-quality miniprep DNA, and they have the Hte phenotype, which confers increased transformation efficiency.1

Even with these many innovations, the expression of heterologous proteins in E. coli can be difficult. Most frequently, failed or insufficient expression of the heterologous gene is caused by the presence of codons that are rarely used in E. coli.2,3 Forced high-level expression of genes with rare codons for the E. coli host can lead to depletion of the endogenous pools of the corresponding tRNAs.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
6. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
7. Simple Isolation of RNA from Tissue and Cultured Cells
8. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
9. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New CodonPlus Cells Correct Codon Bias Rich Genomes

(Date:7/1/2015)... ... July 01, 2015 , ... It is now possible to separate two ... photon emission computed tomography (SPECT) according to researchers from Amen Clinics, UCLA, Thomas ... brain scans and is believed to be the largest brain imaging study ever, will ...
(Date:7/1/2015)... and BANGALORE, India , July 1, ... company that uses next generation sequencing technology to empower ... A. Storrer as chief executive officer. In his ... be responsible for all strategy, business expansion and worldwide ... has served as Strand,s chairman and CEO since its ...
(Date:7/1/2015)... (PRWEB) , ... July 01, 2015 , ... A newly ... new ideas to lower the barriers to innovation in life sciences R&D. The Pistoia ... to two cash prizes of US$15,000 and a period of expert mentorship from a ...
(Date:7/1/2015)... NEW YORK , July 1, 2015 /PRNewswire/ ... G ), a global leader in designing, ... the Aecus Innovation Award in recognition of their ... operational efficiencies in UCB,s global business services (GBS) ... acknowledge service providers and clients that work together ...
Breaking Biology Technology:Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 2Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 4Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 3Pistoia Alliance Launches Startup Challenge 2Pistoia Alliance Launches Startup Challenge 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 2UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 5
... , WAYNE, Pa., July 17 ... a full service multinational clinical research organization (CRO) that ... and patient registries to many of the world,s leading ... previously announced sale of the Company,s U.S. business to ...
... life,sDHA Now ... ... DHA omega-3 innovator and maker of the life,sDHA ™ brand of DHA, Martek Biosciences, ... The Food Chain and Animal Health (SCFCAH) of the European Union for additional novel food ...
... , , SAN DIEGO and ... SAI ) announced today that Anthony Hess, Ph.D., will be ... BU (HSBU) effective Aug. 3. Hess will be responsible ... health customers, including the Departments of Defense, Health and Human Services ...
Cached Biology Technology:Encorium Group Completes Sale of its U.S. Business to Pierrel Research USA; Announces Management Changes 2Encorium Group Completes Sale of its U.S. Business to Pierrel Research USA; Announces Management Changes 3Encorium Group Completes Sale of its U.S. Business to Pierrel Research USA; Announces Management Changes 4EU Committee Approves New Food Applications for Martek's life'sDHA™ 2EU Committee Approves New Food Applications for Martek's life'sDHA™ 3Anthony Hess to Lead SAIC Health Solutions Business Unit 2Anthony Hess to Lead SAIC Health Solutions Business Unit 3
(Date:6/30/2015)... -- To bolster its efforts and commitment to fighting cyber ... addition of two new team members. ... David Raviv will act as head of strategic alliances. ... providing the most secure solutions for the enterprise market. ... Technologies, a provider of security and management products, which was ...
(Date:6/25/2015)... SALT LAKE CITY , June 25, 2015 /PRNewswire/ ... the first steps toward a healthier, happier life. That,s ... app is designed to do. The cutting-edge, portable health ... and offers real-life solutions to help improve your lifestyle ... Sellers Association awards dinner and gala Wednesday night, USANA,s ...
(Date:6/24/2015)... , June 22, 2015 ... announced the addition of the "Huawei Ascend ... report to their offering. Huawei,s ... to the opposite direction than Apple,s and Samsung,s ... fingerprint sensor developed by Fingerprint Cards, a main ...
Breaking Biology News(10 mins):HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3@USANAinc's Personalized Nutrition Program Wins Industry Innovation Award 2Huawei Ascend Mate 7 Fingerprint Sensor - Reverse Costing Analysis 2
... physical barrier against infection but skin cells themselves can mount ... polypeptides (AMPs). , As overt infection in the skin is ... only help fight infection, but play a role in preventing ... appearing online on June 15 in advance of print publication ...
... manipulate computer cursors for fruit juice rewards have revealed ... specifying movements required for given actions, but rather the ... distinction is significant, said the researchers, because it yields ... planning in this higher brain region, called the lateral ...
... only mammals to feed exclusively on blood, including human blood, ... a study published today in the open access journal BMC ... recorded human breathing sounds much better than human participants could. ... and the authors of the study propose that the bats ...
Cached Biology News:The brain's executive is an 'event planner' 2
RABBIT ANTI NEOPTERIN...
MOUSE ANTI HUMAN MBP (67-74) Immunogen: Human myelin basic protein...
NORMAL DONOR DONKEY SERUM...
... Green reagent (F-2902) was developed in collaboration ... permit measurement of the kinetics of Fc ... burst directly in the phagovacuole.2 The Fc ... serum albumin (BSA) that has been covalently ...
Biology Products: