Navigation Links
New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes


High-level expression of heterologous proteins

Carsten-Peter Carstens Julie Bonnardel Anna Waesche Ronda Allen
Stratagene

Codon bias is a significant obstacle for efficient expression of heterologous genes in E. coli hosts. In GC-rich genomes, such as mammals, rare arginine codons (AGG or AGA) and the proline codon (CCC) most frequently affect bacterial gene expression. For such situations, Stratagene introduces BL21-CodonPlus-RP cells* to make protein expression more reliable. This strain contains a ColE1-compatible plasmid that encodes extra copies of the argU and proL tRNA genes and is able to rescue expression of genes restricted by either AGG/ AGA codons or CCC codons.

The E. coli BL21 strains offer many advantages for expression of heterologous proteins. Derived from E. coli B, these strains naturally lack the Lon protease and are engineered to be deficient for the OmpT protease. The BL21(DE3) and the BL21(DE3)pLysS hosts permit induced expression of T7 RNA polymerase. When used in conjunction with pET-type vectors, the expressed T7 RNA polymerase provides significant levels of transcription of heterologous genes. Stratagenes BL21-Gold competent cells** are another member of the BL21 family of cells that feature two additional advantages: They are EndA1 deficient, which allows preparation of high-quality miniprep DNA, and they have the Hte phenotype, which confers increased transformation efficiency.1

Even with these many innovations, the expression of heterologous proteins in E. coli can be difficult. Most frequently, failed or insufficient expression of the heterologous gene is caused by the presence of codons that are rarely used in E. coli.2,3 Forced high-level expression of genes with rare codons for the E. coli host can lead to depletion of the endogenous pools of the corresponding tRNAs.
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
6. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
7. Simple Isolation of RNA from Tissue and Cultured Cells
8. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
9. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New CodonPlus Cells Correct Codon Bias Rich Genomes

(Date:3/27/2015)...  Neogen Corporation (NASDAQ: NEOG ) announced today ... plan in accordance with Securities and Exchange Commission rule ... Neogen Corporation, is a minority owner of this entity ... Herbert does not have control of this entity, ... only for a portion of the shares owned by ...
(Date:3/27/2015)... , March 27, 2015  CASI Pharmaceuticals, Inc. (Nasdaq: ... acquisition, development and commercialization of innovative therapeutics addressing cancer ... with a commercial focus on China ... 12 months ended December 31, 2014.  ... or ($0.05) per share, for the three months ended ...
(Date:3/26/2015)... The Market Element LLC, a leading ... with Designing Gig LLC to provide website design, development, ... “Forging this alliance with Designing Gig represents a pivotal ... a comprehensive, end-to-end, digital solution for our clients, which ... Chuck Miller, president of The Market Element. “For small ...
(Date:3/26/2015)... ReliantHeart, Inc., an innovative supplier of advanced ... precision transit-time flow measurement solutions, are collaborating on a ... of the HeartAssist5® Left Ventricular Assist Device ... mobility and peace of mind. , The Transonic ... draw 1/6th of the power required by earlier flow ...
Breaking Biology Technology:Neogen CEO adopts 10b5-1 Trading Plan 2CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 2CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 3CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 4CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 5CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 6The Market Element LLC and Designing Gig LLC Form Strategic Alliance 2LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 2LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 3
... HEIDELBERG, Germany , April 27 ... as well as the cluster management,s collaborative project, a ... solution will provide high-tech cluster or single companies with ... projects. The suite focuses on the core needs of ...
... ... Solution for German Medical Systems Biology Initiative to Study Infectious Diseases of Fungal Pathogens. ... (PRWEB) April ... and life science research, today announced that Genedata Phylosopher has been adopted by the ...
... Two months after launching a strategic review of its character licensing business, ... to sell United Media Licensing for $175 million to Iconix Brand ... , In conjunction ... late Charles Schulz , who created the beloved Peanuts characters, which represent ...
Cached Biology Technology:Web-Based Management Suite Supports Complex Research Consortia 2German MedSys Project Adopts Genedata Phylosopher for Research Informatics 2German MedSys Project Adopts Genedata Phylosopher for Research Informatics 3Scripps Announces Sale of United Media's Licensing Business for $175 Million 2Scripps Announces Sale of United Media's Licensing Business for $175 Million 3
(Date:3/20/2015)... Mar. 19, 2015 Research and Markets ( ... Geometry - Global Strategic Business Report" report to their ... Hand Geometry in US$ Thousands. The report provides separate comprehensive ... Japan , Europe , ... and Rest of World. Annual estimates and forecasts ...
(Date:3/20/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/vkzvcs/iris_biometrics ) ... Global Strategic Business Report" report to their offering. ... in US$ Thousands. The report provides separate comprehensive analytics for ... , Europe , Asia-Pacific ... Latin America . Annual estimates and ...
(Date:3/18/2015)... , March 18, 2015 As mobile ... new revolutionary smart wallets and apps continue to be introduced ... entrenched in the mobile payment industry in focus today are:  ... Ltd. (NYSE: BABA ), Apple Inc. (NASDAQ: ... and Facebook Inc. (NASDAQ: FB ) ...
Breaking Biology News(10 mins):Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 2Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 3Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 4Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 5Revolutionary Smart Wallet & Mobile Payment Choices Change the Game for Consumers - Company Begins Shipping Newest Digital Wallet to Customers 6
... Chemicals in the environment that mimic estrogen can strongly ... research to be presented at the 2012 International Zebrafish ... Wisconsin, reveals that these substances may act even earlier ... embryonic development. Estrogenic compounds in the environment are ...
... 21, 2012A new study by researchers at St. Michael,s Hospital ... as previously thought and that it may even provide some ... connections made between fructose intake and rates of obesity," said ... this research suggests that the problem is likely one of ...
... The rise of ocean infrastructure development to tap energy ... will require more pile driving, the practice of pounding ... floor to support energy turbines and other structures. But ... fish and other marine animals., Many scientists and regulators ...
Cached Biology News:Environmental estrogens affect early developmental activity in zebrafish 2Environmental estrogens affect early developmental activity in zebrafish 3New evidence in fructose debate: Could it be healthy for us? 2Turning down the dial: Ocean energy development with less sound 2Turning down the dial: Ocean energy development with less sound 3Turning down the dial: Ocean energy development with less sound 4
...
...
For mRNA Differential Display with three one-base anchored oligo-dT primers and rationally designed arbitrary 13mers. These kits feature the latest generation of isotopic mRNA Differential Display t...
Request Info...
Biology Products: