Navigation Links
Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection,,,of Mammalian Cells


Mycoplasma PCR Primer Set for fast and easy detection of mycoplasma contamination

Leslie S. Arrington
Yale University

D. R. Staggs
Genetic Applications LLC

Heinz Miller
Stratagene

Mycoplasma contamination of mammalian cell cultures can substantially reduce the potency of transfection reagents.1 Cells must be free of mycoplasma contamination before the optimal amounts of LipoTAXI transfection reagent and DNA can be accurately determined. The use of Stratagenes Mycoplasma PCR Primer Set*, provides assurance that cells are free of mycoplasma contamination and able to maximally express transfected DNA.

The efficient introduction of foreign DNA into mammalian cells is vital to many areas of biological research. The ability to study the effect of introducing a gene or oligonucleotide of interest in a cell line may depend on the effectiveness of the transfection reagent. Stratagenes LipoTAXI transfection reagent can be used to achieve efficient transfections in a wide variety of mammalian cells. Using simple protocols, researchers can determine the optimal combination of LipoTAXI transfection reagent and plasmid DNA for a particular cell type.2 However, mycoplasma contamination can interfere with transfection using lipid, DEAE-dextran, CaPO4 and adenovirus-mediated methods.1 The resulting low transfection efficiencies may go undetected since antibiotic-resistant mycoplasma can exist in cell cultures without noticeable visual effects on the cells.3

In the typical protocol for optimizing transfection conditions, the amount of LipoTAXI reagent is increased at various concentrations of plasmid DNA that encodes for a reporter gene. Expression of the reporter
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A New PCR-based Mycoplasma Detection Method
2. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. Combat RNase Contamination in the Lab
5. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
6. Testing Compressed Air Lines for Microbiological Contamination
7. Testing Compressed Air Lines for Microbiological Contamination
8. Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results
9. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
10. Effectene Transfection Reagent
11. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
Post Your Comments:
(Date:4/17/2015)... Fremont, CA (PRWEB) April 17, 2015 ... http://www.medispend.com ) for its 20 Most Promising Pharma and ... based on MMIS’s MediSpend global compliance software platform for ... companies is selected by a panel of experts and ... promote technology entrepreneurship. “MMIS’s MediSpend Platform has been on ...
(Date:4/17/2015)... April 17, 2015 Global Stem ... GTCbio Stem Cell Summit 2015 April 27 - 29, ... latest developments in all areas of stem cell research ... stem cells and regenerative medicine. , The conference ... as well as translational research in stem cell therapies, ...
(Date:4/16/2015)... OSAKA, Japan , April 16, ... (CiRA) of Kyoto University and Takeda Pharmaceutical Company ... together to develop clinical applications of induced pluripotent ... diabetes mellitus, neurological disorders and cancer immunotherapy. The ... is designed to expedite multiple research projects for ...
(Date:4/16/2015)... April 16, 2015 Proove Biosciences ... Buzz of BIO Contest for the 2015 BIO ... the 2015 BIO International Convention will take place June ... During the Buzz of BIO contest, industry leaders will ... present at the conference. The community voting period began ...
Breaking Biology Technology:MMIS Chosen by CIO Review as Top 20 Most Promising Pharma and Life Science Technology Solution Providers 2015 2Global Stem Cells Group To Join GTCbio Stem Cell and Regenerative Medicine Summit in Boston, April 27-29, 2015 2Global Stem Cells Group To Join GTCbio Stem Cell and Regenerative Medicine Summit in Boston, April 27-29, 2015 3CiRA and Takeda Enter 10-year Collaboration on iPS Cell Research 2CiRA and Takeda Enter 10-year Collaboration on iPS Cell Research 3Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 2Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 3
... A. Mirkin has mass-produced the 2008 Summer Olympics logo -- ... centimeter of space. , Mirkin and his colleagues printed ... a new printing technique, called Polymer Pen Lithography (PPL), that ... device. , The PPL technique, to be published Aug. ...
... 14 Keck Graduate Institute,(KGI) in collaboration with ... center, announced the launch of its new Certificate ... fellows. KGI has,secured funding to allow a limited ... the program with scholarships that will cover tuition., ...
... (Nasdaq: NLTX ), today announced financial results for the ... loss for the three months ended June 30, 2008 was ... $0.11 per share, for,the same period of 2007. Net operating ... million or $0.28 per share compared to $2.2 million,or $0.16 ...
Cached Biology Technology:Northwestern chemists take gold, mass-produce Beijing Olympic logo 2City of Hope Helps KGI Launch New Management Training Program for Scientists 2City of Hope Helps KGI Launch New Management Training Program for Scientists 3Nile Therapeutics, Inc. Reports 2008 Second Quarter Financial Results 2Nile Therapeutics, Inc. Reports 2008 Second Quarter Financial Results 3Nile Therapeutics, Inc. Reports 2008 Second Quarter Financial Results 4
(Date:3/26/2015)... , March 26, 2015 ... family, social, recreation and athletic club, today announced it ... to allow freedom of movement for members and staff, ... "Following a comprehensive process, we selected FST,s IMID Access ... and convenience for our members and staff, in addition ...
(Date:3/24/2015)... , March 24, 2015   NexID Biometrics ... with critical security-authentication needs, today announced the beginning of ... (FFD) solution. The company, based in ... at the three-day Connect:ID Expo, which began here today ... NexID said version 2.0 of its SDK boosts the ...
(Date:3/23/2015)... 2015   HOYOS Labs , a leading global ... that the Company will demonstrate multiple biometrics-based technologies under ... at Connect:ID on March 23 through 25, 2015, in ... highlight the IEEE Biometric Open Protocol Standard; free biometrics-based ... access control system. BOPS was invented by HOYOS Labs ...
Breaking Biology News(10 mins):Granite Club First Private Club in North America to Implement FST Biometrics Secure Access Solution, as Part of Multi-million Dollar Expansion 2NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3
... of years ago, humans began scrubbing off and discarding ... white kernel beneath. Now, for the first time, scientists ... rice processing, called rice bran, significantly lowers blood pressure ... The team reports their findings in the March 8 ...
... gnawing its way into a carcass and staying inside to feed ... Columbia (Canada) believe the Hagfish's gruesome method of feeding may cause ... build up of CO2 produced by the fish, which could explain ... up to 7% CO2 (350 × that found in normal air). ...
... with Affymetrix, Inc., have designed a chip ?the GeneChip® ... suggest ways to better manage them. By helping determine ... citrus ?genes that are associated with taste, acidic content ... to researchers for rectifying existing problems and making improvements ...
Cached Biology News:How nice, brown rice: Study shows rice bran lowers blood pressure in rats 2UCR researchers design chip that can improve citrus varieties 2UCR researchers design chip that can improve citrus varieties 3