Navigation Links
Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection,,,of Mammalian Cells


Mycoplasma PCR Primer Set for fast and easy detection of mycoplasma contamination

Leslie S. Arrington
Yale University

D. R. Staggs
Genetic Applications LLC

Heinz Miller
Stratagene

Mycoplasma contamination of mammalian cell cultures can substantially reduce the potency of transfection reagents.1 Cells must be free of mycoplasma contamination before the optimal amounts of LipoTAXI transfection reagent and DNA can be accurately determined. The use of Stratagenes Mycoplasma PCR Primer Set*, provides assurance that cells are free of mycoplasma contamination and able to maximally express transfected DNA.

The efficient introduction of foreign DNA into mammalian cells is vital to many areas of biological research. The ability to study the effect of introducing a gene or oligonucleotide of interest in a cell line may depend on the effectiveness of the transfection reagent. Stratagenes LipoTAXI transfection reagent can be used to achieve efficient transfections in a wide variety of mammalian cells. Using simple protocols, researchers can determine the optimal combination of LipoTAXI transfection reagent and plasmid DNA for a particular cell type.2 However, mycoplasma contamination can interfere with transfection using lipid, DEAE-dextran, CaPO4 and adenovirus-mediated methods.1 The resulting low transfection efficiencies may go undetected since antibiotic-resistant mycoplasma can exist in cell cultures without noticeable visual effects on the cells.3

In the typical protocol for optimizing transfection conditions, the amount of LipoTAXI reagent is increased at various concentrations of plasmid DNA that encodes for a reporter gene. Expression of the reporter
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A New PCR-based Mycoplasma Detection Method
2. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. Combat RNase Contamination in the Lab
5. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
6. Testing Compressed Air Lines for Microbiological Contamination
7. Testing Compressed Air Lines for Microbiological Contamination
8. Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results
9. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
10. Effectene Transfection Reagent
11. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
Post Your Comments:
(Date:12/19/2014)... iLab Solutions, the global leader in core facility ... the new Director of Institutional Implementations and Sarah Becker ... new leadership positions were created to support iLab’s mission ... continues to meet the needs of its growing customer ... been deployed at over 450 core facilities across more ...
(Date:12/19/2014)... 19, 2014 The empty capsules ... rising ageing population and technological innovations in the ... industries are catalysing the growth of the empty ... http://www.marketsandmarkets.com/Market-Reports/empty-capsules-market-218018190.html , Scope of Report , The ... as new entrants/smaller firms to gauge the pulse ...
(Date:12/19/2014)... -- Decision Resources Group finds that the Asia ... through 2023 as the aging population, increasing adoption of ... all spur procedure volumes. Growth will be particularly rapid ... and India , where rising ... a larger proportion of the population. Other ...
(Date:12/19/2014)... , Dec. 18, 2014   ISN has ... Safety Performance Indicators publication series. With data ... activity reported in 2013 within its online contractor management ... safety key performance indicator (KPI) statistics. ... U.S. publication is available in two industry ...
Breaking Biology Technology:iLab Solutions Announces Two New Leadership Positions to Meet the Needs of Its Growing Customer Base 2Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 2Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 3Empty Capsules Market Hit to Reach $1,820 Million by 2019 - New Research Report by MarketsandMarkets 4Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 2Asia Pacific Dental Biomaterial Market Will Grow Strongly Through 2023 3ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3
... Legislature are bogged down in a budget fight, just like ... is in Wisconsin. But there's an important distinction between the ... the few areas of agreement, not a source of perpetual ... and Governor Tim Pawlenty are behind schedule in most ways ...
... Congress as well as the Wisconsin Legislature, it is becoming ... research to persuade fellow lawmakers that such science is unethical. ... public opinion about its relative merits is beginning to carry ... of life and the promise of science, the House of ...
... could offer treatment for the underlying process not ... second Governor's Business Plan Contest on Tuesday, ... , ,Mithridion, a University of Wisconsin-Madison spinoff, emerged as ... for the judges. Even before that, co-founder Jeff Johnson, ...
Cached Biology Technology:Feuds between UW and the Legislature should end for good of the state 2Feuds between UW and the Legislature should end for good of the state 3With ethics guidelines, political consensus emerging on stem-cell research 2With ethics guidelines, political consensus emerging on stem-cell research 3Alzheimer's drug firm and more winners named in Wisconsin business-plan contest 2Alzheimer's drug firm and more winners named in Wisconsin business-plan contest 3Alzheimer's drug firm and more winners named in Wisconsin business-plan contest 4
(Date:12/19/2014)... , Dec. 18, 2014   LaunchKey , the ... the post-password and Internet of Things era, today announced ... The venture round was led by Metamorphic Ventures with ... Venture Partners, VegasTechFund, and others.  LaunchKey has raised $4 ... to expand its team and bring LaunchKey to market ...
(Date:12/17/2014)... Dec. 16, 2014 Valencell, a leader in ... biometric technology to industry leaders such as Intel, Jabra, ... validated, biometric wearable products. These products will be showcased ... Las Vegas . "Our ... flexible and robust – with the clinical data to ...
(Date:12/11/2014)... , Dec. 09, 2014 Research and ... ( http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has ... in the APAC Region 2015-2019"  report to their ... in this market is advances in technology. With ... upgrade biometric solutions to the latest standard that ...
Breaking Biology News(10 mins):LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... Humans are having an effect on Earth,s ecosystems but it,s ... the planet we are causing. Now you can add an ... The only question is how large of an impact will ... issue of Science (Dec. 16, 2011), Arizona State ...
... Ill. Clinical gene therapy may be one step closer, ... molecules. A group of University of Illinois researchers, led ... short spiral-shaped proteins can efficiently deliver DNA segments to cells. ... Chemie. "The main idea is these are new ...
... identified a gene mutation that researchers estimate dates back to ... yet discovered. , Researchers with the Ohio State University Comprehensive ... J. Solove Research Institute led the study and estimate that ... ago. Only a mutation seen in cystic fibrosis that arose ...
Cached Biology News:Researchers assess effects of a world awash in nitrogen 2Let's do the twist: Spiral proteins are efficient gene delivery agents 2Let's do the twist: Spiral proteins are efficient gene delivery agents 3Scientists discover second-oldest gene mutation 2
... The Vybrant Apoptosis Assay Kit #4 ... changes that occur in the permeability of ... of both YO-PRO-1 and propidium iodide nucleic ... the plasma membranes of apoptotic cells and ...
Request Info...
... Western Blot Analysis Kit is an ... developed by Biochain. It detects protein ... or yoctomole (10 -24 M) ... parts: Millennium Enhancer, protein interaction system ...
Request Info...
Biology Products: