Navigation Links
Mycobacterium intracellulare

/b>

  1. Add 1 g plasmid DNA to 100 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. Transfer mixture into a prechilled cuvette.
  2. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O Voltage (V) 2,500 V Time constant (T) 5 ms
  4. Transfer cells to 1 ml of 7H9 medium and grow statically for 16-20 h.
  5. To disperse cells, bath sonicate cultures twice for 30 s each time. Dilute suspension in 0.9% NaCl supplemented with 0.1% Tween 80.
  6. Plate on selective agar.
Expected Results: Transformation efficiency up to 106 transformants/g of DNA.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Mycobacterium avium
2. Mycobacterium smegmatis
3. Mycobacterium tuberculosis
4. Mycobacterium avium
5. Mycobacterium intracellulare
6. Mycobacterium smegmatis
7. Mycobacterium tuberculosis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Mycobacterium intracellulare

(Date:5/21/2015)... , May 21, 2015 /PRNewswire/ - RepliCel ... clinical stage regenerative medicine company focused on the ... upcoming poster presentation at the International Society for ... for chronic Achilles tendinosis currently in a Phase ... May 29 th from 5:30 PM to ...
(Date:5/20/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/x4b7kw/top_technologies ... Technologies in HW_Technical Insights" report to their offering. ... technology trends in the Health and Wellness sector and ... have an impact in the year 2015. The research ... 10 health and wellness technologies that are anticipated to ...
(Date:5/20/2015)... 2015 The global genotyping market is ... the forecast period of 2015 to 2020 to reach ... in drug discovery and development process and to provide ... addition to this,heavy investments by key manufacturers to offer ... propelling market growth. Browse 113 market data tables & ...
(Date:5/20/2015)... , May 20, 2015  Haemonetics Corporation (NYSE: ... , President & CEO, will present at The Jefferies ... on June 1 st , 2015 at ... access Mr. Concannon,s presentation live via webcast at: ... Haemonetics (NYSE: HAE ) is a global healthcare ...
Breaking Biology Technology:RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 2RepliCel to Present Unique Autologous Cell Treatment for Achilles Tendinosis at International Society for Cellular Therapy Conference 3Top Technologies in the Health and Wellness Industry 2015 2Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 2Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 3Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 4Genotyping Market Growing at 22.3% CAGR to 2020: Research Based on Technology, Applications, Opportunities, Challenges and Key Players. 5
... , SAN DIEGO, Nov. 24, 2009 Arena Pharmaceuticals, ... company is scheduled to present at the Piper Jaffray 21st Annual ... Time (8:30 a.m. Pacific Time) at the New York Palace Hotel ... Officer, is scheduled to provide an overview of the company, including ...
... , BOZEN, Italy, ... formed a new Canadian joint venture with a group ... under the,name Health Robotics Canada, Inc. and will market, ... concept, which includes the,revolutionary product offerings i.v.STATION(TM), i.v.SOFT(TM), and ...
... ... Abstracts Service (CAS), the global expert on chemical information, reports that China,s ... in chemistry. China trailed Japan,s patent office, the World Intellectual Property Organization ... than a decade, but passed the USPTO in 2005, WIPO in 2006, ...
Cached Biology Technology:Arena Pharmaceuticals to Present at the Piper Jaffray 21st Annual Health Care Conference 2Arena Pharmaceuticals to Present at the Piper Jaffray 21st Annual Health Care Conference 3Health Robotics Continues its Global Expansion With December 09 ASHP's Launch of Joint Venture in Canada 2Health Robotics Continues its Global Expansion With December 09 ASHP's Launch of Joint Venture in Canada 3China Leads All Nations in Publication of Chemical Patents According to CAS, the World's Most Authoritative Publisher of Chemical Information 2China Leads All Nations in Publication of Chemical Patents According to CAS, the World's Most Authoritative Publisher of Chemical Information 3
(Date:4/14/2015)... YORK , April 14, 2015  HYPR ... IDentity Online (FIDO ® ) Alliance tm , ... specifications. FIDO members commit to share technology and ... methods that are interoperable, more secure and private, ... enable biometric identity verification that protects sensitive user ...
(Date:4/13/2015)... 2015 According to a ... Market Forecast & Opportunities, 2020", the global biometrics market ... 14% till 2020. The biometrics market is garnering ... and review of biometric management systems. The constant ... with greater efficiency, are resulting in increasing installation ...
(Date:4/10/2015)... Research and Markets ( http://www.researchandmarkets.com/research/cjk4gb/security ) has ... - NEC" report to their offering. ... to supply a range of IT security management and ... on the development of a Big Data and cloud ... Asia-Pacific region is a core ...
Breaking Biology News(10 mins):HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2NEC Security Competitive Profile 2015 2
... As the desert Southwest becomes hotter and drier, ... dominated by mesquite trees, such as Prosopis velutina, and ... researchers has found. In a "leaf-to-landscape" approach, the ... across entire ecosystems to quantify how well grasslands, compared ...
... Bonheur Professor Russell Chesney, M.D. believes he knows what was ... Christmas Carol." Based on detailed descriptions of both the ... hypothesizes that Tiny Tim suffered from a combination of rickets ... March 5 edition of Archives of Pediatrics and Adolescent ...
... now 20 years old, and its network of protected areas, known ... start, the network now includes some 26 000 protected sites and ... significant areas of sea. It is widely considered to be the ... criteria. The review has been published in the newly ...
Cached Biology News:Mesquite trees displacing Southwestern grasslands 2Mesquite trees displacing Southwestern grasslands 3Mesquite trees displacing Southwestern grasslands 4Mesquite trees displacing Southwestern grasslands 5Building the European Union's Natura 2000 -- the largest ever network of protected areas 2
Mouse monoclonal [6911] to E. coli K99 pilus ( Abpromise for all tested applications)....
...
... HyPer is the first fully genetically ... detection of intracellular hydrogen peroxide (H2O2), one ... Developed on the basis of yellow fluorescent ... Escherichia coli protein OxyR (OxyR-RD), HyPer demonstrates ...
Alexa Fluor 647 anti-mouse Qa-2...
Biology Products: