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Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

ides a straight-forward link between the genomics/proteomics biomarker discovery experiments and high throughput, quantitative MS validation. Low cost, rapid method development allows for the MRM validation of many candidate biomarkers without the need for protein or peptide standards, relieving a key bottleneck. This strategy can be employed to narrow candidates to a smaller set to which labeled synthetic peptides are made as internal standards for further quantitative MS analysis. Results from this MS assay should be further validated using biological assays.

References

1. Anderson, N.L. and Anderson, N.G., Molecular & Cellular Proteomics, 2003, 2(1): 50.

2. Anderson, N.L. and Hunter C.L., Molecular and Cellular Proteomics, published December 6, 2005.


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