Navigation Links
Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

estion step of the plasma preparation was explored by taking one sample, splitting it in two and performing parallel depletions, followed by a further split of each to perform parallel digestions. All samples were then assayed and correlated (Figure 5, left). Initial results suggest that these types of sample preparation techniques can be performed in a highly reproducible manner.

MIDAS TRAQ System optimized for the MIDAS Workflow

To enable the MIDAS workflow at optimum performance levels, Applied Biosystems/MDS SCIEX has introduced the MIDAS TRAQ LC/MS/MS system. The system combines the reproducibility and ease-of-use of the new Tempo nano MDLC system with the high sensitivity and dynamic range of the 4000 Q TRAP hybrid triple quadrupole linear ion trap system. The system is also fully compatible with iTRAQ reagents for protein biomarker discovery.

Conclusions

A single period multiplexed MRM assay consisting of 137 MRM experiments representing 53 plasma proteins (including 38 cardiovascular disease biomarkers) has been developed and tested in digested human plasma.2 Very good reproducibility between replicates and across sample preparations was obtained, highlighting the potential of this method for higher throughput quantitative biomarker early stage validation.

A key strategy to the design of these methods was the use of the MIDAS workflow for developing the MRM transitions to previously undetected proteins and improving MRM selection and sensitivity to others. With this straightforward strategy, proteins in the top 5 orders of the protein abundance curve in human plasma can be quantitatively profiled in a high throughput manner with minimal sample and minimal sample preparation.

This same strategy can be applied to proteins of lower abundance, using additional upfront sample preparation.

The MIDAS workflow prov
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
2. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
3. Mx4000 Multiplex Quantitative PCR System*,PPP
4. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
5. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
8. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
9. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
10. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
11. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
Post Your Comments:
(Date:10/22/2014)... 22, 2014 Research and Markets ... "Global DNA Diagnostics Market (Product types, Application, Technology, ... Trends, Company Profiles, Demand, Insights, Analysis, Research, Report, ... report to their offering. This ... techniques, end users and Geography) Global Size, Industry ...
(Date:10/22/2014)... Massachusetts (PRWEB) October 22, 2014 ... announced the release of a new infographic, Understanding ... to create a beautifully designed, easy to follow informative ... transmission, areas of outbreak, symptoms and prevention. , "As ... discovered that the 2014 Ebola outbreak represents not only ...
(Date:10/22/2014)... According to new market research ... (Consulting, Integration, Deployment & Support), by Service Model ... Private, Others (Hybrid & Community)) - Global Forecast ... the Cloud Professional Services Market into various sub-segments ... It also identifies the drivers and restraints for ...
(Date:10/22/2014)... HONG KONG , Oct. 22, 2014  aTyr ... today that rare disease expert John C. McKew ... Dr. McKew brings more than two decades of expertise ... National Institutes of Health, Wyeth Research and Genetics Institute, ... lead aTyr,s efforts to expand and translate its novel ...
Breaking Biology Technology:Global DNA Diagnostics Market (Product types, Application, Technology, End User and Geography) - Forecast to 2020 2Global DNA Diagnostics Market (Product types, Application, Technology, End User and Geography) - Forecast to 2020 3Involution Studios' Ebola Infographic Provides Key Disease Information and Statistics at a Glance 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... Phlo Corporation,(Pink Sheets: PHCP) (the "Company") announced today ... vitality beverage, AQISS(TM),will be significantly expanded with the ... beverage distributors in the San Francisco Bay Area., ... in principal with Morris,Distributing providing for the distribution ...
... July 14 Cepheid (Nasdaq:,CPHD) today announced that it ... June 30, 2008, on Thursday, July 24, 2008 after ... a management presentation at 1:30 p.m. Pacific,Time on Thursday, ... the live,webcast, please visit Cepheid,s website at http://www.cepheid.com/investors ...
... Liquids) will be held in Göteborg, Sweden on October 13-14, 2008 ... discussing commercialization of BTL. Technological advancements & worldwide growth of BTL ... insights on biomass to liquid industry prospect , ... ...
Cached Biology Technology:Phlo Affiliate Announces Significant Distribution Expansion In California 2Cepheid Schedules Fiscal 2008 Second Quarter Financial Results Announcement and Webcast 2Will New Technological Advances Offer Fresh Capabilities for Commercialization and Enhance BTL Investments? 2Will New Technological Advances Offer Fresh Capabilities for Commercialization and Enhance BTL Investments? 3
(Date:10/16/2014)... challenged conventional thinking on how the bowel lining develops ... how bowel cancer starts. , The researchers produced evidence ... the ,crypts, that are a feature of the bowel ... bowel cancer development, a controversial finding as scientists are ... 3D imaging technologies, Dr Chin Wee Tan and Professor ...
(Date:10/16/2014)... -- NXT-ID, Inc. (NASDAQ: NXTD and ... focused on the growing mobile commerce market announces that ... Smart Wallet, at the 13 th annual Future ... conference on the intersection of technology and the economy. ... founder and publisher of the Strategic News Service™, the ...
(Date:10/15/2014)... German Gauß-Allianz has admitted Johannes Gutenberg University Mainz ... Rhineland-Palatinate science hub continues to maintain a significant ... , "The acceptance of Johannes Gutenberg University ... is a milestone in the national and international ... The state of Rhineland-Palatinate can be proud that ...
Breaking Biology News(10 mins):Cryptic clues drive new theory of bowel cancer development 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 3Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 3
... American Physical Society (APS) announces a new public access ... libraries in the United States full use of all ... to the first issue in 1893, a collection including ... this access at no cost to participating public libraries, ...
... D.C (July 29, 2010) While pundits write obituaries for ... already being felt across the world. We are losing species ... before seen. We need new tools and strategies ... and EcoAdapt are proud to announce the launch of their ...
... agriculture,s hardest workers as key pollinators for one-third of ... last half-century, with die-offs in recent years stumping scientists ... National Science Foundation (NSF) multi-institutional grant co-led by ... 10 natural history bee collections across the United States. ...
Cached Biology News:CAKE offers way forward for climate change adaptation 2New $1.5 million NSF grant to track bee declines and pollination 2
... Microarray Dot Blot Kits overcome this limitation ... over a wide range in low-density protein ... They are ideal for performing reverse-phase ... samples for the presence of low abundant ...
... easy-to-use kits for arraying proteins of ... common lab equipment. DiscoverLight Protein Array ... your protein, cell lysate or antibody ... kits include specially designed nitrocellulose membranes ...
Request Info...
... StabilZyme AP Conjugate Stabilizer is an ... bovine protein and other non-toxic stabilizing ... acid), pH 5.5 6.5. ... 0.02% methylisothiazolone and 0.02% bromonitrodioxane as ...
Biology Products: