Navigation Links
Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

estion step of the plasma preparation was explored by taking one sample, splitting it in two and performing parallel depletions, followed by a further split of each to perform parallel digestions. All samples were then assayed and correlated (Figure 5, left). Initial results suggest that these types of sample preparation techniques can be performed in a highly reproducible manner.

MIDAS TRAQ System optimized for the MIDAS Workflow

To enable the MIDAS workflow at optimum performance levels, Applied Biosystems/MDS SCIEX has introduced the MIDAS TRAQ LC/MS/MS system. The system combines the reproducibility and ease-of-use of the new Tempo nano MDLC system with the high sensitivity and dynamic range of the 4000 Q TRAP hybrid triple quadrupole linear ion trap system. The system is also fully compatible with iTRAQ reagents for protein biomarker discovery.

Conclusions

A single period multiplexed MRM assay consisting of 137 MRM experiments representing 53 plasma proteins (including 38 cardiovascular disease biomarkers) has been developed and tested in digested human plasma.2 Very good reproducibility between replicates and across sample preparations was obtained, highlighting the potential of this method for higher throughput quantitative biomarker early stage validation.

A key strategy to the design of these methods was the use of the MIDAS workflow for developing the MRM transitions to previously undetected proteins and improving MRM selection and sensitivity to others. With this straightforward strategy, proteins in the top 5 orders of the protein abundance curve in human plasma can be quantitatively profiled in a high throughput manner with minimal sample and minimal sample preparation.

This same strategy can be applied to proteins of lower abundance, using additional upfront sample preparation.

The MIDAS workflow prov
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
2. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
3. Mx4000 Multiplex Quantitative PCR System*,PPP
4. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
5. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
8. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
9. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
10. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
11. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
Post Your Comments:
(Date:9/16/2014)... phones to cars and flashlights, batteries play an ... companies constantly are seeking ways to improve battery ... using a water-based solution, researchers at the University ... efficient nuclear battery that could be used for ... in automobiles and also in complicated applications such ...
(Date:9/16/2014)... September 16, 2014 BlueInGreen® ... most efficient method for delivering dissolved gases into ... Inc. as a manufacturer’s representative firm to ... dissolution products for municipal water and wastewater applications ... , H2Flow was founded in 1992 and serves ...
(Date:9/16/2014)... MILLBROOK, N.Y. , Sept. 16, 2014  Donald Fresne, ... the appointment of Stephan Vagner , PhD to its ... the University of Toulouse, France , performed ... Germany , and at Inserm, the French institute of ... Inserm and works both at the Curie Institute, Orsay, ...
(Date:9/16/2014)...  The Council for Entrepreneurial Development (CED), the ... country, today released a mid-year update to its ... It showed that in the first half of ... in the technology, life science, advanced manufacturing and ... variety of sources. Significant investment in technology companies and ...
Breaking Biology Technology:BlueInGreen® Expands into Canada – Signs Representative Agreement with H2Flow Equipment Inc. 2New Appointment to the Scientific Advisory Committee at Egenix, Inc. 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 2CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 3CED's Mid-Year Update to its Innovators Report Shows NC Startups Attracting Investment From Across the Globe with Several High-Profile Investment Deals, IPOs and Acquisitions Signaling Strength of Entrepreneurial Sector 4
... KNSY ) is scheduled to participate in a ... at the 2007 RBC Capital Markets,Healthcare Conference., Douglas ... panel,discussion on Thursday, December 13th at 8:00 a.m. ET ... York. The conference is by invitation only,however individuals can ...
... Promoting Advanced Technology,Industries Careers, HARRISBURG, Pa., ... from the Harrisburg area will continue learning ... special collaboration,between several local schools and the ... by the Harrisburg University of Science and,Technology, ...
... Dendreon Corporation (Nasdaq:,DNDN) today announced that the Company ... senior vice president of clinical affairs and chief ... a decade of oncology,drug development and patient treatment ... to the filing of the company,s Biologics License,Application ...
Cached Biology Technology:Capital Area Biotechnology Partnership Receives $160,000 State Investment 2Dendreon Promotes Dr. Mark Frohlich to Senior Vice President of Clinical Affairs and Chief Medical Officer 2Dendreon Promotes Dr. Mark Frohlich to Senior Vice President of Clinical Affairs and Chief Medical Officer 3
(Date:9/16/2014)... Proteins are responsible for practically all vital functions ... reactions, forward signals, transport particular substances and control ... that proteins do not function independently of each ... you examine the protein networks, you find many ... Falter-Braun from TUM,s Chair of Plant Systems Biology. ...
(Date:9/16/2014)... , Sept. 16, 2014  Cross Match ... management solutions, announced today the launch of its ... authenticate the identity of an individual using their ... Verifier Sentry rapidly reads credential documents with embedded ... by matching the biometric to a live scan ...
(Date:9/16/2014)... season of bounty. Fungi of dizzying variety erupt from ... search of elusive but delectable wild mushrooms. Part of ... hunt, and their mysterious not-quite-meat, not-quite-vegetable qualities that belie ... you are eating known to science? , The Fungi ... some estimates range up to 10 million species, only ...
Breaking Biology News(10 mins):Good networkers make prime targets 2Cross Match Launches New Identity Management Handheld Solution 2What's for dinner? Rapidly identifying undescribed species in a commercial fungi packet 2What's for dinner? Rapidly identifying undescribed species in a commercial fungi packet 3
... Slugs are every gardener,s enemy: They can destroy overnight, ... don,t like all plants in the same way they ... posed by the botanist and founder of Chemical Ecology, Ernst ... More than a hundred years later chemists from Friedrich-Schiller-University Jena ...
... hands and arms through innovative robotic and virtual reality-based video ... Sergei Adamovich, a biomedical engineer. Thanks to a ... Adamovich is developing better ways to rehabilitate people who have ... Preliminary laboratory results appear promising: ...
... 2010) Acidity is increasing in some regions of the ... ocean, where it is now recognized that increased levels of ... more acidic. These more acidic conditions in key parts of ... to new research published in the journal Estuaries and ...
Cached Biology News:Self-defense strategies of moss 2NJIT engineer helps stroke patients regain motor functions in hands, arms 2Changing Chesapeake Bay acidity impacting oyster shell growth 2Changing Chesapeake Bay acidity impacting oyster shell growth 3
... a new technology for sensitive detection ... amounts of DNA (5). This process ... modify the DNA, followed by PCR ... distinguish methylated from unmethylated DNA. The ...
... m7G(5')ppp(5')G (Cap Analog) is used for the ... in vitro transcription reactions. Substitution of cap ... GTP in an in vitro transcription reaction ... structure into a large fraction of the ...
... The iEMS Incubator/Shaker HT ... Thus, the iEMS Incubator/Shaker HT ... as DNA hybridization and primer ... is available as a one-cabinet ...
The iEMS Incubator/Shaker is a high performance microplate incubator and orbital shaker. Its superior temperature control and efficient orbital shaking reduce incubation times. -...
Biology Products: