Navigation Links
Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

ptide by MRM drives the acquisition of MS/MS to confirm the peptide sequence and thus definitively identity of the detected peptide (Figure 3). Because of the high specificity and sensitivity of MRM, these experiments possess better signal/noise than full scan MS signals and can be used to dig deeper into a sample. The hybrid triple quadrupole linear ion trap technology of the 4000 Q TRAP system is the only platform that enables this combination of specific MRM detection with high sensitivity ion trap MS/MS. The peptide MRM transitions can be determined either experimentally from previously obtained proteomics data or in silico by using the MIDAS workflow software designer. From the protein sequence, theoretical tryptic peptides are determined and the fragmentation pattern of the peptide by MS/MS is predicted (Figure 4). This strategy is key to developing MRMs with good S/N to low abundance proteins that are often not observed in regular LC/MS/MS methods. In addition, putative biomarkers identified from a genomics based discovery platform can also be included in this validation strategy as all that is required is the sequence of the protein. No peptide or protein standards are required for method development.

High Quantitative Reproducibility:

A key component to the early and late stage validation of biomarkers in any body fluid is the ability to prepare and analyze many samples in parallel in a highly reproducible manner. The reproducibility of the LC-MRM method was assessed by measuring 10 LC-MRM replicates on the same sample. With no internal standard correction, the reproducibility of the peak areas was better than 10% for the majority of the MRM transitions (Figure 5, right). The average %CV for all MRM peaks was 8.6% for depleted plasma (loading equivalent of 10 nL of plasma) and 11.7% for undepleted plasma (loading 1 nL equivalent).

The reproducibility of both the depletion and dig
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
2. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
3. Mx4000 Multiplex Quantitative PCR System*,PPP
4. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
5. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
8. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
9. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
10. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
11. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
Post Your Comments:
(Date:1/22/2015)... Dr. Greg Leyer of UAS Labs recently was ... Pre-Conference seminar on probiotics in San Diego, CA. , ... conference for health care professionals. This year’s pre-conference seminar was ... in health. Dr. Leyer spoke about the emerging topics and ...
(Date:1/22/2015)... 22, 2015 Diagenode, Inc., a ... the Bioruptor® and complete solutions for epigenetics research, ... immunoprecipitation, alleviating the need for manual processing. The ... for ChIP of histones or transcription factors and ...
(Date:1/22/2015)... , Jan. 22. 2015  Varian Medical Systems (NYSE: ... has been honored for its commitment to sustainability with inclusion ... Varian is the highest ranked healthcare equipment company among the ... during the World Economic Forum at Davos, Switzerland ...
(Date:12/25/2014)... report “Service Quality Management and Telco Customer Experience Management ... Advancements, Worldwide Forecasts & Analysis (2014-2019)” defines and segments ... revenues. , Browse 152 market data tables, 19 ... “Service Quality Management (SQM) and Telco Customer Experience Management ...
Breaking Biology Technology:Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Varian Honored Among World's 100 Most Sustainable Corporations 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 2Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 3Service Quality Management Market worth $2.1 Billion & Telco Customer Experience Management Market worth $2.3 Billion by 2019 – New Report by MarketsandMarkets 4
... April 11 Galderma Pharma S.A., a,global ... that its U.S. holding company, Galderma Laboratories, ... Inc. (Nasdaq:,CGPI). As a result of the ... of Galderma Laboratories., "We are pleased ...
... venture capitalists, investment bankers, international start-up, CEOs, business development ... ... the globe, MASSAPEQUA, ... national investment conference organizer,and BioEnterprise Corporation, the Cleveland-based bioscience business,formation, ...
... April 11 Powersafe,Technology Corp. (OTC Bulletin Board: ... Technologies, Inc., a privately held Delaware,corporation. Pursuant to ... with a wholly-owned subsidiary of Powersafe in exchange ... issued to,shareholders of Amplification and reserved for the ...
Cached Biology Technology:Galderma Completes Acquisition of CollaGenex 2Galderma Completes Acquisition of CollaGenex 3Galderma Completes Acquisition of CollaGenex 4IBF Conferences Inc*International Business Forum and BioEnterprise Corporation present the 2nd Annual Global Healthcare Investing Conference 2IBF Conferences Inc*International Business Forum and BioEnterprise Corporation present the 2nd Annual Global Healthcare Investing Conference 3Powersafe Technology Corp. Recapitalizes, Executes Merger Agreement with Amplification Technologies, and Invests in Amplification 2
(Date:12/19/2014)... 2014 Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) has ... America Perimeter Security Systems Market" report to their offering. ... North American perimeter security market is estimated to grow at ... U.S. market holds a larger share in this market, ...
(Date:12/19/2014)... Dec. 18, 2014   LaunchKey , the first decentralized ... and Internet of Things era, today announced the close ... round was led by Metamorphic Ventures with participation from ... VegasTechFund, and others.  LaunchKey has raised $4 million to ...
(Date:12/17/2014)... The Defense Logistics Agency is insourcing its efforts ... microcircuits from entering into its supply chain. ... initiative dubbed DNA marking. The capability will validate the ... the supply chain. The new quality control measures will ...
Breaking Biology News(10 mins):Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 2Defense Logistics Agency Launches DNA Marking Capability to Strengthen Microcircuit Supply Chain 3
... conducted in the context of the Swiss National Research Programme ... for the first time that internal exposure of humans to ... of the Summer and Fall 2004, 2005 and 2006 (3 ... birth at the University Women,s Hospital in Basel. The participants ...
... November 1, 2010 School meal programs play ... are "the anchor" of comprehensive school nutrition services ... performance, according to an updated joint position paper ... Dietetic Association (ADA) and the School Nutrition Association ...
... Publishers Association (OPA) today released a new study, "Biometric Evaluation: ... that 90 percent of participants notice OPA Ad Units in ... users who fixated(1) on the OPA Ad Units during the ... with the rest of the webpage content. The study further ...
Cached Biology News:Exposure of humans to cosmetic UV filters is widespread 2Exposure of humans to cosmetic UV filters is widespread 3Comprehensive nutrition services vital to children's health 2Online Publishers Association Unveils Biometric and Eye Tracking Research to Assess the Effectiveness of New OPA Ad Units 2Online Publishers Association Unveils Biometric and Eye Tracking Research to Assess the Effectiveness of New OPA Ad Units 3
Arginine vasopressin receptor (AVP-V2), rat. The peptide used for immunization has no significant similarity with AVP V1a or V1b receptors....
... to WinList come straight from the folks ... like automatic region positioning, N-Stat regions, and ... suggestions, thoughtful conversations, and careful design. Of ... on are still there, too. WinList leads ...
Recognizes the VZV nucleocapsid by indirect immunofluorescence and immunoprecipitation. Shows cross-reactivity to HSV....
Adenylate kinase isoenzyme 6...
Biology Products: