Navigation Links
Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

ptide by MRM drives the acquisition of MS/MS to confirm the peptide sequence and thus definitively identity of the detected peptide (Figure 3). Because of the high specificity and sensitivity of MRM, these experiments possess better signal/noise than full scan MS signals and can be used to dig deeper into a sample. The hybrid triple quadrupole linear ion trap technology of the 4000 Q TRAP system is the only platform that enables this combination of specific MRM detection with high sensitivity ion trap MS/MS. The peptide MRM transitions can be determined either experimentally from previously obtained proteomics data or in silico by using the MIDAS workflow software designer. From the protein sequence, theoretical tryptic peptides are determined and the fragmentation pattern of the peptide by MS/MS is predicted (Figure 4). This strategy is key to developing MRMs with good S/N to low abundance proteins that are often not observed in regular LC/MS/MS methods. In addition, putative biomarkers identified from a genomics based discovery platform can also be included in this validation strategy as all that is required is the sequence of the protein. No peptide or protein standards are required for method development.

High Quantitative Reproducibility:

A key component to the early and late stage validation of biomarkers in any body fluid is the ability to prepare and analyze many samples in parallel in a highly reproducible manner. The reproducibility of the LC-MRM method was assessed by measuring 10 LC-MRM replicates on the same sample. With no internal standard correction, the reproducibility of the peak areas was better than 10% for the majority of the MRM transitions (Figure 5, right). The average %CV for all MRM peaks was 8.6% for depleted plasma (loading equivalent of 10 nL of plasma) and 11.7% for undepleted plasma (loading 1 nL equivalent).

The reproducibility of both the depletion and dig
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
2. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
3. Mx4000 Multiplex Quantitative PCR System*,PPP
4. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
5. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
8. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
9. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
10. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
11. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
Post Your Comments:
(Date:8/28/2015)... IRVINE, CA (PRWEB) , ... August 28, 2015 ... ... leader in Personalized Medicine, presented two studies at the International Spine Intervention ... on July 28th, 2015 through to August 1st, 2015. With a focus on ...
(Date:8/28/2015)... ... 2015 , ... Since the initial launch of FireflySci Inc. (FFS) in early 2015, the company ... Images such as the Fonz in a lab coat and Large Marge have been ... spectroscopy field. , FireflySci is proud to be the only cuvette manufacturer is ...
(Date:8/27/2015)... 2015 /PRNewswire/ - Portage Biotech Inc. ("Portage") (OTC ... Pharmaceutical Holding Company Limited (Biohaven), announced today that ... of BHV-0223, a glutamate modulating agent. Biohaven filed ... recently obtained clearance from the U.S. Food and ... Portage holds 54% equity interest in Biohaven, a ...
(Date:8/27/2015)... 27, 2015 HUYA Bioscience International (HUYA) today announced ... Japan Society of HTLV-1, held this week at the ... of the Society is to promote research on HTLV-1 ... medical technology and research related to these fields to ... "HUYA is proud to support this prestigious conference," said ...
Breaking Biology Technology:Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 2Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 3Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 4Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 4HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 2HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 3HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 4
... Imaging,Diagnostic Systems, Inc., (OTC Bulletin Board: ... systems, will exhibit CT Laser Mammography (CTLM(R)),technology ... Cancer,Conference, March 1-5, in Las Vegas, Nevada. ... (Logo: http://www.newscom.com/cgi-bin/prnh/20040412/IMDSLOGO ), "Attending ...
... SAN DIEGO, Feb. 27 NuVasive, Inc. (Nasdaq:,NUVA), a ... surgical treatments for the spine, announced today,that Kevin O,Boyle, ... present at the Canaccord Adams, Small-Cap Orthopedics,Conference at The ... on Tuesday, March 4, 2008 at 8:10 a.m. PT., ...
... Ocera Therapeutics, a,biopharmaceutical company focused on the ... a broad range of,gastrointestinal and liver diseases, announced ... company as Chief Medical Officer. Prior to joining,Ocera, ... Officer and Vice,President of Clinical Affairs at Napo ...
Cached Biology Technology:CT Laser Mammography Technology to be Featured at Interdisciplinary Breast Cancer Conference 2NuVasive to Present at Canaccord Adams' Small-Cap Orthopedics Conference 2NuVasive to Present at Canaccord Adams' Small-Cap Orthopedics Conference 3M. Scott Harris, M.D., Joins Ocera Therapeutics, Inc., as Chief Medical Officer 2
(Date:8/5/2015)... According to a new market research report, ... (BFSI, Airport, IT & Telecom, Utilities, Education, Defense & ... published by MarketsandMarkets, defines and segments the Physical Identity ... global PIAM Market is estimated to grow from $272.2 ... a CAGR of 14.9 % from 2014 to 2019. ...
(Date:8/3/2015)... 2015 Synaptics, Inc. (Nasdaq: SYNA ), ... that members of the executive management team will present ... Technology Leadership Forum on Tuesday, August 11, 2015 at ... at the Sonnenalp Resort in Vail, CO. ... forward-looking information. An audio webcast of the event will ...
(Date:7/31/2015)... 31 de julio de 2015 La 10 th ... celebrará por medio de BGI del 22 al 25 de octubre ... Este año, la conferencia celebra su décimo aniversario. ... convertido en una de las reuniones anuales más influyentes del ... las reuniones más dinámicas, entusiastas y mejores a nivel científico. ...
Breaking Biology News(10 mins):Physical Identity and Access Management Market Worth $546.2 Million by 2019 2Physical Identity and Access Management Market Worth $546.2 Million by 2019 3Physical Identity and Access Management Market Worth $546.2 Million by 2019 4La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3
... major research findings at the 11th International Coral Reef Symposium ... African Dust Poses Threat to Coral Reefs and Human ... Caribbean and the Americas may be a threat to marine ... study by researchers with the U.S. Geological Survey, Oregon State ...
... Researchers from Harvard Medical School and Brandeis University have ... what types of genes are necessary for brain development. ... are published July 4th in the open-access journal PLoS ... sequencing of entire genomes and the discovery of RNA-interference ...
... the biggest challenges facing humanity is to halt the destruction ... task that must be urgently addressed and in which we ... is our duty to do all we can for the ... researchers Harold Mooney and Peter Raven, regarded as the architects ...
Cached Biology News:The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 2The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 3The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 4The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 5The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 6The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 7The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 8The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium 9Researchers reveal types of genes necessary for brain development 2BBVA Foundation Awards for Biodiversity Conservation 2BBVA Foundation Awards for Biodiversity Conservation 3BBVA Foundation Awards for Biodiversity Conservation 4
NATIVE, ENZYME PORCINE TRYPSIN...
G-Protein Coupled Receptor Assay, For 500 assays. SPA. Category: Drug Screening & Cellular Assays, Assays ....
EDTA, disodium salt, 1 kg. Ethylene diaminetetraacetic acid.Dihydrate. Assay: 99.0-101.0%, nuclease-free.Risks: 36, 37, 38 ; Safety Precautions: 22, 36, 37. Category: Nucleotides & Enzymes & Biochemi...
Alkaline Phosphatase Stabilizer Solution, 1 L...
Biology Products: