Navigation Links
Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

quality MRM methods to obtain the best sensitivity and dynamic range for peptides from a panel of putative protein biomarkers for cardiovascular disease. For candidate proteins previously detected in proteomics experiments, MRMs were designed based on peptide MS/MS spectra. For the remaining candidates, MRMs were developed either by in silico design, based on gene or protein sequences, or by targeted, direct detection in plasma using the MIDAS workflow.

The MIDAS workflow provides a straight-forward link between the genomics/proteomics biomarker discovery experiments and high throughput, quantitative MS validation, because no previous experimental data on the individual proteins of interest is required to develop an assay. This quantitative LC-MRM method profiles 53 high and medium abundant proteins in human plasma (Figure 1). Proteins down to ~1 μg/mL concentration in plasma can be reliably detected in both digested and depleted/digested human plasma, producing a dynamic range of ~5 orders of magnitude in this single method (Figure 1, inset). Peptide MRM measurements in plasma digests thus provide a robust platform for quantitative protein biomarker early stage validation.

MIDAS Workflow for Method Development:

During an MRM experiment, the triple quadrupole capability of the mass spectrometer transmits the parent ion and subsequent fragment ion with high sensitivity and selectivity. The first mass analyzer is set to transmit only the mass of the peptide parent ion (Q1 at unit resolution) into the collision cell. Only one of the sequence ions of the peptide, generated by fragmentation in the collision cell, is passed through the second mass analyzer (Q3 at unit resolution) to the detector (Figure 2).

This MRM experiment can be used as a survey scan in a MIDAS workflow to selectively detect low level peptides in complex mixtures. The detection of the pe
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
2. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
3. Mx4000 Multiplex Quantitative PCR System*,PPP
4. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
5. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
8. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
9. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
10. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
11. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
Post Your Comments:
(Date:12/17/2014)... York, NY (PRWEB) December 16, 2014 ... significant changes since 2013, which is why IBISWorld updated ... this industry continues to benefit from an intensified focus ... the proposal for new emission standards for power plants ... industry operators. According to IBISWorld Industry Analyst Sarah Kahn, ...
(Date:12/17/2014)... Bellingham, Washington, USA (PRWEB) December 16, 2014 ... Kirstin Baum, manager at Philipps- Universität Marburg’s Department ... optics and photonics, in the newly released ... 2015-2016. “And never be too shy to ask ... STEM (science, technology, engineering, and mathematics) occupations ranging ...
(Date:12/17/2014)... RIDGE, N.J. (PRWEB) December 17, 2014 ... (Euronext: IPN; ADR: IPSEY), today ... mg (referred to as Somatuline®) was approved by ... the treatment of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) in ... locally advanced or metastatic disease to improve progression-free ...
(Date:12/17/2014)... 17, 2014 This report is ... Tubing industry. For an overview analysis, the report ... industry chain structure, industry overview, policy analysis, and ... industry has witnessed rapid development with key ... acquisitions. This report mentions quartz tubing upstream raw ...
Breaking Biology Technology:Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 2Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 3Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 2Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 82020 Quartz Tubing Market: Global Development & Forecasts Analysis Now at DeepResearchReports.com 2
... 28, 2011 , Spherix Incorporated (NASDAQ: ... for therapy in diabetes, metabolic syndrome and atherosclerosis, and ... supplement, biotechnology and pharmaceutical companies – today announced the ... of shares of its common stock together with warrants ...
... Bioceuticals, a wholly owned subsidiary of Mistral Ventures (MILV.pk), ... pulmonary disease market (COPD) with the completed acquisition of ... medicine was developed for the treatment of COPD, the ... Chronic Bronchitis. We believe that there is no other ...
... of Colorado Boulder study shows that huge amounts of ... promote healthy heart growth, results that may have implications ... Leinwand and her research team found the amount of ... oils -- in the blood of Burmese pythons one ...
Cached Biology Technology:Spherix Announces Closing of Private Placement of $1.25 Million, Discusses Use of Proceeds 2Spherix Announces Closing of Private Placement of $1.25 Million, Discusses Use of Proceeds 3Nature's Bioceuticals Enters Chronic Obstructive Pulmonary Disease Market After Acquiring Breakthrough Breathing Formula 2Nature's Bioceuticals Enters Chronic Obstructive Pulmonary Disease Market After Acquiring Breakthrough Breathing Formula 3CU-Boulder python study may have implications for human heart health 2CU-Boulder python study may have implications for human heart health 3CU-Boulder python study may have implications for human heart health 4
(Date:12/17/2014)... , Dec. 15, 2014  HITLAB SM ... Practices (GCP) audit to confirm its adherence to ... This accomplishment enables HITLAB to conduct regulated smart ... highest principles for patient safety and research quality. ... healthcare access, quality, and delivery with innovative technology," ...
(Date:12/17/2014)... , Dec. 16, 2014 Research and ... the addition of the "Global Chemical Sensor ... http://photos.prnewswire.com/prnh/20130307/600769 ... the increasing demand for medical sensors in biomedical ... data for quick and correct diagnosis during surgical ...
(Date:12/15/2014)... 2014 Research and Markets ( ... "Global Facial Recognition Market 2015-2019" report ... http://photos.prnewswire.com/prnh/20130307/600769 Facial recognition is a ... recognition system measures the overall facial feature of ... and the distance between eyes. Facial recognition is ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3
... ( Caretta caretta ) and fishing gear such as longline ... effects of climate change on these animals. In order to ... for fishermen, the scientists are proposing that fishing in the ... areas more than 35 nautical miles from land. Populations ...
... colon cancer cells, may help identify patients at risk ... by Vanderbilt-Ingram Cancer Center researchers. The findings, published ... help personalize treatments for colon cancer the second ... by identifying patients most likely to benefit from ...
... MADISON A team of University of ... friendly process that selectively converts gamma-valerolactone, a biomass ... The simple process preserves about 95 percent ... little hydrogen input, and captures carbon dioxide under ...
Cached Biology News:How can accidental captures of loggerhead turtles be reduced? 2Gene signature may improve colon cancer treatment 2Gene signature may improve colon cancer treatment 3New process yields high-energy-density, plant-based transportation fuel 2
RABBIT ANTI ISOCITRATE DEHYDROGENASE...
Recombinant Feline IL-5...
dithiothreitol (DTT) Reactive Dyes and Chemicals Thiol-Reactive Dyes, Haptens and Quenchers Reducing Agents...
Recombinant Feline IL-2, CF...
Biology Products: