Navigation Links
Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

quality MRM methods to obtain the best sensitivity and dynamic range for peptides from a panel of putative protein biomarkers for cardiovascular disease. For candidate proteins previously detected in proteomics experiments, MRMs were designed based on peptide MS/MS spectra. For the remaining candidates, MRMs were developed either by in silico design, based on gene or protein sequences, or by targeted, direct detection in plasma using the MIDAS workflow.

The MIDAS workflow provides a straight-forward link between the genomics/proteomics biomarker discovery experiments and high throughput, quantitative MS validation, because no previous experimental data on the individual proteins of interest is required to develop an assay. This quantitative LC-MRM method profiles 53 high and medium abundant proteins in human plasma (Figure 1). Proteins down to ~1 μg/mL concentration in plasma can be reliably detected in both digested and depleted/digested human plasma, producing a dynamic range of ~5 orders of magnitude in this single method (Figure 1, inset). Peptide MRM measurements in plasma digests thus provide a robust platform for quantitative protein biomarker early stage validation.

MIDAS Workflow for Method Development:

During an MRM experiment, the triple quadrupole capability of the mass spectrometer transmits the parent ion and subsequent fragment ion with high sensitivity and selectivity. The first mass analyzer is set to transmit only the mass of the peptide parent ion (Q1 at unit resolution) into the collision cell. Only one of the sequence ions of the peptide, generated by fragmentation in the collision cell, is passed through the second mass analyzer (Q3 at unit resolution) to the detector (Figure 2).

This MRM experiment can be used as a survey scan in a MIDAS workflow to selectively detect low level peptides in complex mixtures. The detection of the pe
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
2. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
3. Mx4000 Multiplex Quantitative PCR System*,PPP
4. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
5. The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!
6. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
7. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
8. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
9. Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
10. Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
11. Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
Post Your Comments:
(Date:5/28/2015)... 2015 Celsion Corporation (the "Company") (NASDAQ: ... from two institutional healthcare investors to purchase an aggregate ... in an at-the-market registered direct offering and a concurrent ... The Company entered into a definitive purchase agreement ... to sell an aggregate of 3,000,000 shares of its ...
(Date:5/28/2015)... 28, 2015 BioNorth Texas (BioNorthTX), ... representing the life sciences industry in northern region ... meeting at The Foundry in Dallas on April ... brought together a broad spectrum of biotech professionals ... organization's early successes in partnering with key life ...
(Date:5/28/2015)... , May 28, 2015 Biscayne ... anti-cancer potential of its growth hormone-releasing hormone (GHRH) technology ... the 2015 ASCO Annual Meeting. The data show that ... Biscayne,s anti-cancer GHRH blockers, is present on many primary ... findings suggest that GHRH antagonists could have broad anti-cancer ...
(Date:5/28/2015)... The Fertility Centers of New England is proud ... taken on a new and expanded role as Medical ... leadership, oversight and quality assurance for the practice. , ... team of experienced reproductive endocrinologists,” said Fertility Centers of ... “Her expertise and compassionate, individualized care have helped set ...
Breaking Biology Technology:Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 2Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 3BioNorthTX Holds Inaugural Board of Directors Meeting 2BioNorthTX Holds Inaugural Board of Directors Meeting 3Data Presented At 2015 ASCO Annual Meeting Shows Biscayne Pharmaceuticals' GHRH Antagonist Target Is Expressed Across All Breast Cancer Subtypes 2Data Presented At 2015 ASCO Annual Meeting Shows Biscayne Pharmaceuticals' GHRH Antagonist Target Is Expressed Across All Breast Cancer Subtypes 3Danielle Vitiello, MD of Fertility Centers of New England, Named New Medical Director 2
... the Chicago Bar Association entitled "Communication Rights-of-Way: Municipal Revenue Generators ... many who attended thought it was a novel idea but ... was that the horse-and-buggy taxes that focused on gasoline, cigarettes, ... more creative to seek out revenue streams that could pay ...
... Wis. - Two southeastern Wisconsin medical informatics ... health records. , ,The agreement between TeraMedica Healthcare ... . of Germantown will merge technology products with the ... images and other patient information. , ,TeraMedica, a privately ...
... Wis. - The state Department of Commerce ... credits under the state's Angel Investor and Venture Fund ... designed to encourage investment in technology and biotechnology companies, ... investors in seed-stage venture capital funds. People that invest ...
Cached Biology Technology:Creativity, innovation extend to municipalities 2Creativity, innovation extend to municipalities 3Creativity, innovation extend to municipalities 4Commerce qualifies seven for investor tax credits 2
(Date:5/5/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or ... mobile commerce market, reminds investors and media that  Mr. ...  at CARTES SECURE CONNEXIONS AMERICA 2015, held in ... The three-day conference is organized into a series of nine ... Global Fraud: Where is the Trust in Cyberspace? ...
(Date:4/27/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... on the growing mobile commerce market, announces that its ... the first week of May, 2015 and will be ... Gino Pereira , Chief Executive ... company as Wocket® enters the consumer market. We would ...
(Date:4/21/2015)... , April 21, 2015 ... evolving government policies are boosting access control systems market ... According to a recently published report by TechSci ... 2020 ", the access control systems market in ... USD1.2 billion by 2020.The access control systems market in ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3
... cells (Teff cells) are involved in activating and directing ... to curb the over-aggressive responses of the T cell ... to mobilize anti-tumor Teff cells in order to better ... and colleagues from Memorial Sloan-Kettering Cancer Center, New York, ...
... Georgia researchers shows that the common practice of killing ... matters worse in some cases. , In a study ... of the Royal Society B: Biological Sciences, post-doctoral researcher ... and UGA Biomedical and Health Sciences Institute researcher, create ...
... exclusively on blood, including human blood, recognize their prey by ... in the open access journal BMC Biology, vampire bats of ... much better than human participants could. Vampire bats feed on ... the study propose that the bats use breathing sounds to ...
Cached Biology News:UGA researchers find that hunting can increase the severity of wildlife disease epidemics 2
... a complete assay system for the measurement ... serum or plasma. The assay incorporates the ... IDS 1,25D RIA followed by quantitation by ... to use reagents, no organic or solvent ...
Rabbit polyclonal to Nck alpha ( Abpromise for all tested applications). entrezGeneID: 4690 SwissProtID: P16333...
anti-achaete...
ATP-binding cassette sub-family D member 4 (Peroxisomal membrane protein 69) (PMP69) (Peroxisomal membrane protein 1-like) (PXMP1-L) (P70R). [Source:Uniprot/SWISSPROT;Acc:O14678] Antigen: Recombi...
Biology Products: