Navigation Links
Lambda DNA Extraction Protocol


This method produces good quality LambdaDNA with yields of 1520 g lgt10 DNA per 450 l of supernatant processed. PLG tubes also can be used in conjunction with other LambdaDNA purification methods.(1)
  1. Grow a 10 ml stock of suitable plating bacteria in L-broth plus Mg2+ (1% Tryptone, 0.5% yeast extract, 0.5% NaCl, 1 mM MgSO4, pH 7.5) overnight at 37C.
  2. Dilute lambda phage stock to l05 pfu per 0.1 ml in SM buffer (0.1 M NaCI, 8 mM MgSO4, 50 mM Tris-Cl, pH 7.5, 0.01% Gelatin), then mix 0.1 ml of the lambda dilution with 0.1 ml plating bacteria in a sterile 13 x 100 mm culture tube and allow phage to adsorb to bacteria for 20 minutes at 37C.
  3. Add 2.5 ml melted top agarose (L-broth plus Mg2+ and 0.6% agarose, 4550C) to the tube, mix well and pour onto a 100 mm L-broth plus Mg2+ plate. Allow top agarose to harden and incubate at 37C for 8 hours. Plaques should be evident at this time.
  4. Chill plate for 15 minutes at 4C, add 3 ml lamda diluent (10 mM Tris-CI, pH 7.5, 8 mM MgSO4) to the plate and incubate overnight at 4C with gentle rocking.
  5. Collect phage-containing lambda diluent to a centrifuge tube and centrifuge at 4,000 x g for 10 minutes, 4C, to pellet debris.
  6. Transfer 450 l of the resultant supernatant to a pre-spun (1500 x g for 12 minutes) PLG 15 ml tube (Light or Heavy), add 4.5 l 1 mg/ml DNase I and 2.0 l 12.5 mg/ml RNase, mix and incubate 30 minutes at 37C.
  7. Add 11.5 l 20% SDS and 4.5 l 10 mg/ml Proteinase K to the sample, mix, and incubate 30 minutes at 37C.
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. A New Lambda Vector for Mammalian Expression
2. Highly Active and Removable AffinityTagged Lambda Phosphatase
3. Lambda DNA Extraction Protocol
4. M13/Phagemid DNA Extraction Protocol
5. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
6. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
7. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
8. M13/Phagemid DNA Extraction Protocol
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. S.E.T.S. Swab Extraction Tube System
11. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Lambda DNA Extraction Protocol

(Date:8/29/2014)... 29, 2014 Local veterinarian, Dr. Keith ... are seeking candidates to participate in an investigational study ... The ultimate goal of this study is to determine ... one or two arthritically affected joints can help reduce ... Candidates for the current investigational study must be older ...
(Date:8/28/2014)... DIEGO, California, USA (PRWEB) August 28, 2014 ... the Department of Physics and Optical Science and Director ... the University of North Carolina at Charlotte, and co-founder ... been elected to serve as the 2015 Vice President ... , SPIE 2014 President Philip Stahl announced recent election ...
(Date:8/28/2014)... converters are important devices in spintronics, an electronic ... of electrons but also on their spin and ... of electric into magnetic signals and vice versa. ... from the Institute of Physics at Johannes Gutenberg ... UK, Prague, and Japan, has for the first ...
(Date:8/28/2014)... August 28, 2014 Sterlitech is proud ... membranes to its stable of products . ... weight cut-offs and include membrane filters with surface charges. ... in customer demand for our membrane process testing equipment, ... of flat sheet membranes,” explains Sterlitech President Mark Spatz. ...
Breaking Biology Technology:Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2A new, tunable device for spintronics 2A new, tunable device for spintronics 3Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2
... NEW YORK, October 14 , - Site ... to,Both FDA and EMEA Drug Approval Documents, ... for authoritative drug safety data, has significantly expanded ... European Medicines,Agency,s (EMEA) European Public Assessment Reports (EPARs). ...
... CARLSBAD, Calif., Oct. 14 GenVault Corporation today,announces ... its Senior Vice,President of Business Development. Mr. Sutherland ... opportunities for partnering,and licensing the company,s products and ... alliances., Mr. Sutherland has more than 25 ...
... Former Director of the Office of National Drug ... Educational Webinar: When Good Medicines Become Bad Drugs, ... (Nasdaq:,CEPH) announced today that it is embarking on ... among patients, caregivers,and the public about the importance ...
Cached Biology Technology:Elsevier's PharmaPendium(TM) Adds European Medicines Agency (EMEA) Documents 2Elsevier's PharmaPendium(TM) Adds European Medicines Agency (EMEA) Documents 3Mark Sutherland Joins GenVault as Senior Vice President, Business Development 2Cephalon Launches National Consumer Awareness Initiative on Appropriate Use of Prescription Opioid Medications 2
(Date:8/28/2014)... news release is available in German . ... water fish, owes its name to a striking pattern of ... types, black cells, reflective silvery cells, and yellow cells emerge ... and arrange as a multilayered mosaic to compose the characteristic ... three cell types have to interact to form proper stripes, ...
(Date:8/28/2014)... MINNEAPOLIS/ST. PAUL (August 28, 2014) Researchers at the ... for facioscapulohumeral muscular dystrophy (FSHD) to be used for ... effectiveness of potential therapies for FSHD. , The research ... Cell Reports . , There is no treatment ... the most common type of muscular dystrophy. FSHD is ...
(Date:8/28/2014)... Aug. 28, 2014  Privacy Advocate and Senior Staff ... Lynch , joins the lineup of biometric and mobile ... Biometrics UnPlugged Executive Summit in Tampa, Florida ... Previously announced speakers include Steven Rahman, Director, Technology and Strategy ... at Experian. The theme of this year,s event is ...
Breaking Biology News(10 mins):How the zebrafish gets its stripes 2How the zebrafish gets its stripes 3UMN researchers find animal model for understudied type of muscular dystrophy 2Biometrics UnPlugged Welcomes the Electronic Frontier Foundation's Jennifer Lynch to the Mobility at the Crossroads of Commerce and Privacy Summit 2
... endometrium (uterine lining) and transplanted into the brains of ... of brain cells damaged by the disease, according to ... The findings are published in the ... are preliminary results, the findings increase the likelihood that ...
... NEW YORK (May 6, 2010) The Wildlife ... of a large, "landscape-wide" conservation approach to protect globally ... study looked at wildlife populations in northern Republic of ... national park, a community-managed reserve, and various logging concessions. ...
... produced the first whole genome sequence of the 3 billion ... that up to 2 percent of the DNA in the ... or in Neanderthals, ancestors. The international research ... Institute (NHGRI), part of the National Institutes of Health, reports ...
Cached Biology News:Endometrial stem cells could repair brain cells damaged by Parkinson's disease 2Study looks at gorillas, elephants and logging in Congo 2Study looks at gorillas, elephants and logging in Congo 3Complete Neanderthal genome sequenced 2Complete Neanderthal genome sequenced 3Complete Neanderthal genome sequenced 4Complete Neanderthal genome sequenced 5
...
LabFax: Immunochemistry...
Recombinant Ovine (Sheep) Growth Hormone, Ultra Pure...
albumin from bovine serum (BSA), 2,4-dinitrophenylated (DNP-BSA)...
Biology Products: