Navigation Links
Isolate and Analyze Total RNA from Cells Harvested by Laser Capture ,,, Microdissection



StrataPrep total RNA microprep kit for very small samples

Karen Dolter Sylvia Norman Jeff Braman

Stratagene

Isolating total RNA from laser capture microdissection samples can be challenging due to the typically small size of the samples. The StrataPrep total RNA microprep kit provides an efficient method to isolate high-quality total RNA from small cell samples. The protocol includes binding of RNA to a solid support, which eliminates the need for organic extraction and alcohol precipitation. The isolated RNA is ready for a variety of quantitative and conventional RT-PCR experiments.

Laser capture microdissection (LCM) enables researchers to collect individual cells from tissue sections.1 This method facilitates the separation of histologically different cells so that proteins, DNA, or RNA can be analyzed from the cells of choice, but isolation of total RNA from very small samples by traditional methods can be difficult and inefficient. Stratagenes StrataPrep total RNA microprep kit is designed for quick and easy isolation of total RNA from 1 to 5 x 105 cells2 and is particularly useful for isolation of RNA from LCM samples. The resulting RNA can be used for both quantitative and conventional RT-PCR analysis.

RT-PCR Analysis of Varying-Abundance Messages

We isolated total RNA from LCM samples from the core of a human brain tumor section. We used this RNA to synthesize cDNA with the prostar first strand RT-PCR kit. PCR was performed using Stratag
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
2. A Better Way to Isolate RNA AND Protein From the Same Sample
3. Isolate It All: siRNA miRNA Total RNA Native Protein
4. Isolate Total RNA and Protein From the Same Sample
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
8. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
9. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
10. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
11. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
Post Your Comments:
(Date:10/17/2014)... Germany , October 17, 2014 /PRNewswire/ ... research in cancer and other diseases will benefit ... Society of Human Genetics annual meeting   ... Ingenuity ®  Knowledge Base greatly exceeds any comparable ... Ingenuity ®  Variant Analysis™ adds fully integrated BIOBASE ...
(Date:10/17/2014)... The Europe Fuel Additives Market report ... with analysis and forecast of revenue. , Browse through ... to get an idea of the in-depth analysis provided. ... the European fuel additives market, and is supported by ... , Fuel additives are chemicals that are used to ...
(Date:10/17/2014)... The report “Feed Antioxidants Market by Type (BHA, ... Aquatic Animals & Others), by Geography - Global Trends ... feed antioxidant market with analyses and projection of the ... feed antioxidants. , The global market for feed antioxidants, ... around $191.1 million in 2013 and is projected to ...
(Date:10/17/2014)... The report "Thermocouples and High End ... Petrochemical, Consumer Utility, Glass, and Aerospace & Defense) ... Analysis & Forecast to 2010 - 2020", defines ... pyrometer market into various sub-segments with an in-depth ... the drivers and restraints for this market with ...
Breaking Biology Technology:QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 2QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 3QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 4QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 5QIAGEN Demonstrates Expanded Bioinformatics Workflows at ASHG; Strong Market Adoption as Researchers Analyze Genomic Data on More Than a Quarter-Million Samples in Secure Private Cloud 6The Europe Fuel Additives Market is estimated to grow to $1,401.4 million by 2018 - New Report by MicroMarket Monitor 2The Europe Fuel Additives Market is estimated to grow to $1,401.4 million by 2018 - New Report by MicroMarket Monitor 3The Europe Fuel Additives Market is estimated to grow to $1,401.4 million by 2018 - New Report by MicroMarket Monitor 4Feed Antioxidants Market Projected To Reach $238.5 Million by 2018 - New Report by MarketsandMarkets 2Feed Antioxidants Market Projected To Reach $238.5 Million by 2018 - New Report by MarketsandMarkets 3Feed Antioxidants Market Projected To Reach $238.5 Million by 2018 - New Report by MarketsandMarkets 4Thermocouples Market Growing at CAGR of 3.70% & High End Optical Pyrometers Market Growing at CAGR of 13.81% - New Report 2Thermocouples Market Growing at CAGR of 3.70% & High End Optical Pyrometers Market Growing at CAGR of 13.81% - New Report 3Thermocouples Market Growing at CAGR of 3.70% & High End Optical Pyrometers Market Growing at CAGR of 13.81% - New Report 4
... the Project on Emerging Nanotechnologies (PEN) at the ... variety of voluntary options available for the oversight ... Voluntary Initiatives, Regulation, and Nanotechnology Oversight: Charting a ... Center for Environmental Policy at American University, provides ...
... content="bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics. Co-development of ... launch in 2012,bioMerieux gains exclusive rights in microbiology,bioMerieux takes equity stake ... in Molecular... -- MARCY L,ETOILE, France and LAUSANNE, Switzerland, Nov. 4, ... ...
... Novavax, Inc. (Nasdaq: NVAX ) announced ... grants under the Internal Revenue Service,s Qualifying Therapeutic Discovery ... Protection and Affordable Care Act of 2010 to provide ... of eligible qualified investments in therapeutic discovery projects during ...
Cached Biology Technology:bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 2bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 3bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 4bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 5bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 6bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 7bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 8bioMerieux and Biocartis Sign Strategic Partnership in Molecular Diagnostics 9
(Date:10/14/2014)... JOLLA, CA – October 14, 2014 – Scientists from ... million from the National Institutes of Health (NIH) to ... prevalent virus-induced hemorrhagic fever disease in Africa. The study ... and why some patients die, while others survive the ... to understand the basic mechanism of how Lassa fever ...
(Date:10/14/2014)... an international team of researchers, led by the Chinese ... journal Nature Genetics a brief genomic history ... of the tomato plant. , The C.M. Rick Tomato ... role in this study by providing seed of both ... study, which builds on the first tomato genome sequence ...
(Date:10/14/2014)... – Over the past decades, we have seen numerous ... the needs of infants for social contact and stimulation ... thrive. , Infancy and childhood are critical ... A generation of research suggests that enriched environments, full ... cognitive function. In contrast, deprivation and stress may compromise ...
Breaking Biology News(10 mins):Scripps Research Institute team receives $6.6 million to investigate deadly Lassa virus 2Institutional rearing may increase risk attention-deficit disorder 2
... the Keck School of Medicine of the University of ... estrogen, the female sex hormone, activates genes in breast-cancer ... to eventually lead to new treatments for the disease. ... of the Keck School,s Department of Biochemistry and Molecular ...
... and PARIS Nov. 15, 2011   ... authentication and endpoint protection solutions, today announced at ... France, that the company has open sourced its ... Open Source Edition (OSE), is free, portable software ...
... A $7.5 million award will help researchers harness ... create new types of antidotes for exposure to pesticides ... Institutes of Health (NIH), extends a previous grant and ... University, where chemists will collaborate with the U.S. Army ...
Cached Biology News:USC researchers discover key aspect of process that activates breast cancer genes 2DigitalPersona Open Sources New FingerJetFX Biometrics Technology for Mobile Devices, PCs and Servers 2DigitalPersona Open Sources New FingerJetFX Biometrics Technology for Mobile Devices, PCs and Servers 3Researchers get $7.5M grant to combat nerve agents 2Researchers get $7.5M grant to combat nerve agents 3
...
... Detection Kit is intended for quantitative detection ... peptides, which can result from the gradual, ... aspartic acid residues during storage or handling. ... the monitoring of charge differences for detection, ...
...
...
Biology Products: