Navigation Links
Isolate and Analyze Total RNA from Cells Harvested by Laser Capture ,,, Microdissection



StrataPrep total RNA microprep kit for very small samples

Karen Dolter Sylvia Norman Jeff Braman

Stratagene

Isolating total RNA from laser capture microdissection samples can be challenging due to the typically small size of the samples. The StrataPrep total RNA microprep kit provides an efficient method to isolate high-quality total RNA from small cell samples. The protocol includes binding of RNA to a solid support, which eliminates the need for organic extraction and alcohol precipitation. The isolated RNA is ready for a variety of quantitative and conventional RT-PCR experiments.

Laser capture microdissection (LCM) enables researchers to collect individual cells from tissue sections.1 This method facilitates the separation of histologically different cells so that proteins, DNA, or RNA can be analyzed from the cells of choice, but isolation of total RNA from very small samples by traditional methods can be difficult and inefficient. Stratagenes StrataPrep total RNA microprep kit is designed for quick and easy isolation of total RNA from 1 to 5 x 105 cells2 and is particularly useful for isolation of RNA from LCM samples. The resulting RNA can be used for both quantitative and conventional RT-PCR analysis.

RT-PCR Analysis of Varying-Abundance Messages

We isolated total RNA from LCM samples from the core of a human brain tumor section. We used this RNA to synthesize cDNA with the prostar first strand RT-PCR kit. PCR was performed using Stratag
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
2. A Better Way to Isolate RNA AND Protein From the Same Sample
3. Isolate It All: siRNA miRNA Total RNA Native Protein
4. Isolate Total RNA and Protein From the Same Sample
5. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
6. Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Analysis of in vitro micronucleus assays using the IN Cell Analyzer 3000 Micronuclei Formation Analysis Module
8. Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000
9. The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies
10. Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000
11. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
Post Your Comments:
(Date:1/22/2015)... 22, 2015 Pipette.com has added the ... their comprehensive portfolio of Eppendorf products. , The Eppendorf ... a Centrifuge 5424/5424 R and receive the following:, ... Research plus or Eppendorf Reference 2 , ...
(Date:1/22/2015)... 2015 Diagenode, Inc., a leading ... Bioruptor® and complete solutions for epigenetics research, recently ... alleviating the need for manual processing. The new ... ChIP of histones or transcription factors and a ...
(Date:1/22/2015)... 22, 2015  Derma Sciences, Inc. (Nasdaq: ... advanced wound care, announces that AMNIOEXCEL® and AMNIOMATRIX®, ... added to the Premier, Inc. Regenerative Skin Grafting ... the AMNIOEXCEL® and AMNIOMATRIX® product lines, which continue ...
(Date:12/24/2014)... Wash. , Dec. 24, 2014   BioLife Solutions , ... and marketer of proprietary clinical grade hypothermic storage ... shipping products for cells and tissues  ("BioLife" or the ... Meeting of Stockholders on May 4, 2015 (the "Annual Meeting"). ...
Breaking Biology Technology:Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 2Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 3Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 4Derma Sciences Expands Access of its AMNIOTIC TISSUE Product Line with New Premier, Inc. Agreement 5BioLife Solutions Sets Date for Annual Meeting of Stockholders 2
... Inc. (OTC Bulletin Board Symbol: CCEL) (the "Company"), ... cord blood banks, today,announced results for fiscal 2007., ... November 30, 2007 were,approximately $17.5 million, up 2% ... November 30, 2006. The Company reported a net ...
... 11 AGA Medical Corporation ("AGA"),announced today it received ... ("ADO II"). AGA also announced the immediate availability,and launch ... the AGA family of,occlusion devices designed to occlude or ... heart defect that occurs when a blood vessel,called the ...
... Feb. 11 Genta,Incorporated (Nasdaq: GNTA ) ... with institutional investors to sell 6 million shares,of ... proceeds totaling,approximately $3.1 million before fees and expenses. ... about February 13, 2008, subject to the,satisfaction of ...
Cached Biology Technology:Cryo-Cell International, Inc. Reports Results for Fiscal 2007 2Cryo-Cell International, Inc. Reports Results for Fiscal 2007 3Cryo-Cell International, Inc. Reports Results for Fiscal 2007 4Cryo-Cell International, Inc. Reports Results for Fiscal 2007 5Cryo-Cell International, Inc. Reports Results for Fiscal 2007 6AGA Medical Corporation Receives European CE Mark Approval for the AMPLATZER(R) Duct Occluder II 2Genta Announces Common Stock Offering of Approximately $3.1 Million 2Genta Announces Common Stock Offering of Approximately $3.1 Million 3
(Date:1/22/2015)... , Jan. 21, 2015  Analyst Report Issued by Small ... Brands report showed that planet-wide, transactions at merchants on the ... the total number of credit, debit, and prepaid cards reached ... were victims of fraudulent card usage in 2012, and credit ...
(Date:12/24/2014)... 2014  Since its launch in December 2014, the 1U™ ... pain of trying to remember their usernames and passwords through ... their smartphones. To assist people who have struggled to remember ... created 1U and focuses on redefining identity, announced today that ...
(Date:12/19/2014)... 18, 2014 Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ) ... North America Perimeter Security Systems Market" report to their ... The North American perimeter security market is estimated to grow ... the U.S. market holds a larger share in this market, ...
Breaking Biology News(10 mins):$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 21U Offers Best Solution to the Username / Password Dilemma: For FREE! 3Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3
... to know if your child had a fever or ... thoroughly cooked, you would, of course, use a thermometer ... temperature within its range. However, it isn,t that simple ... systemstiny, channel-lined devices used in medical diagnostics, DNA forensics ...
... Since most parents in the US are employed, there ... choices for themselves and their children. How parents cope with ... choice coping strategies are the subjects of a study in ... and Behavior . Findings suggest that better work conditions may ...
... What about tree power? It turns out that it,s ... in trees for University of Washington researchers to run ... in an upcoming issue of the Institute of ... "As far as we know this is the first ...
Cached Biology News:NIST calculations may improve temperature measures for microfluidics 2Work conditions impact parents' food choices 2Electrical circuit runs entirely off power in trees 2Electrical circuit runs entirely off power in trees 3