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Imaging and Analysis of DIGE Product Information Sheet, Rev B

the targeted Analysis Set spots using Bio-Rads ProteomeWorks Plus spot cutter. PDQuest software will direct automatic excision of the targeted proteins, track the excised spots through microtiter plate digestion and mass spectrometry protein identification, and allow automatic annotation of the gel image with the resulting protein identifications.


Reference
Unlu M et al., Difference gel electrophoresis: a single gel method for detecting changes in protein extracts, Electrophoresis 18, 20712077 (1997)

Cy is a trademark of Amersham Biosciences. SYPRO is a trademark of Molecular Probes, Inc.


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... III digests duplex DNA in a 3'-> ... or nick, producing stretches of single-stranded DNA. ... Exonuclease III proceeds at a uniform rate ... rate of exonucleolytic excision of deoxyribonucleotides by ...
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