Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B

nt amount of saturated pixels, select Total Density in Gel Image instead.

Compare the Cy3 and Cy5 images with the Scatter Plot view (Analyze>Scatter Plot). Activate and inactivate normalization to view the effect of normalization.

Multi-Channel Viewer
The multi-channel viewer (View>Multi-Channel Viewer) gives an initial visualization of matched and unique spots in the three Cy-labeled images, but it is not necessary for the analysis. Overlay the desired images and select the Auto-Scale Image When Assigned checkbox to adjust the images to similar brightness and contrast.

The following color scheme is suggested: blue for the Cy2 image, green for the Cy3 image, and red for the Cy5 image.

Analysis
Use Analysis Set Manager to create analysis sets that contain spots exhibiting significant protein regulation between the Cy3 and Cy5 samples (Analyze>Analysis Set Manager>Create). The Spot Review tool (Analyze>Spot Review) can be used to review these analysis sets and to verify that all the spots in each analysis set are defined as desired. A subset of these spots can be selected by clicking on their graphs and then selecting Create Analysis Set to establish the desired DIGE analysis set.

Preparative Gels
Carry out a separate spot detection on the SYPRO Ruby stained gel. Use the Automated Detection and Matching dialog box, and disable steps 3 and 4. It is not necessary for preparative gels to match perfectly to the corresponding DIGE gels in most cases the preparative and analytical gel images will appear very different. However, it should be possible to locate the proteins of interest that were targeted in the analytical gels in the
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:2/27/2015)... 2015 A paper published today, " ... " in Science Translational Medicine, demonstrates ... reduce the risk associated with investing in the ... levels of funding for developing so-called "orphan" drugs. ... collaboration between scientists at the National Center for ...
(Date:2/27/2015)... 2015 Bionomics Limited (ASX:BNO, ADR:BMICY) is to ... BNC105 in patients with metastatic renal cancer at the ... . The data will be presented by Dr. ... Comprehensive Cancer Center in California ... data identifies Ferritin and IL-8 as two baseline biomarkers ...
(Date:2/27/2015)... , Feb. 27, 2015  Steep Hill, the industry ... and internationally, intends to open a full service medical ... Mexico , bringing advanced scientific tools and methodology ... Steep Hill is currently the only laboratory licensed ... potency and contaminant testing in order to meet the ...
(Date:2/26/2015)... , Feb. 26, 2015  23andMe, the leading personal ... Black as Privacy Officer and Corporate Counsel. Black ... privacy laws as well as health care regulations. As ... will be responsible for reviewing, updating and enhancing the ... U.S. and abroad. She joined the company January 5, ...
Breaking Biology Technology:New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 2New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 3New Financing Technique May Hold Key to Unlocking Funding for Rare Diseases 4New BNC105 Biomarker Data to be Presented at US Cancer Conference 2New BNC105 Biomarker Data to be Presented at US Cancer Conference 3New BNC105 Biomarker Data to be Presented at US Cancer Conference 4New BNC105 Biomarker Data to be Presented at US Cancer Conference 5Steep Hill First to Receive Provisional Approval to Establish Laboratory Services for Cannabis Testing in State of New Mexico 223andMe Appoints Kate Black as Privacy Officer and Corporate Counsel 2
... equipment manufacturer Gehl Co. has developed a new ... functions using a compact, wireless remote control pendant from outside ... hand-held pendant can be used to raise, lower, extend and ... , ,"Our goal was to add a new dimension of ...
... years of living on government pay, I'm finally banking a few bucks. ... few others, and getting paid by a law firm and an accounting ... to speak, which might surprise those folks who poked fun at "the ... I didn't admit it: I miss the flags, fanfare and fun of ...
... The Medical College of Wisconsin has been awarded ... Medical Countermeasures against Radiation, an effort to take the college's ... radiation. , ,The institute is one of seven centers being ... Diseases (NIAID) to establish a plan for identifying and ...
Cached Biology Technology:Reading Tommy Thompson's diary: Imagining unspoken thoughts about 2008 2Reading Tommy Thompson's diary: Imagining unspoken thoughts about 2008 3Medical College getting $18.1 million to study radiation damage treatments 2
(Date:2/24/2015)... YORK , Feb. 24, 2015 This report ... The report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... . Annual estimates and forecasts are provided for the ... provided for these markets. Market data and analytics are derived ...
(Date:2/24/2015)... , Feb. 24, 2015 Research and ... the addition of the "Global 2D Gesture ... The analysts forecast the Global ... CAGR of 32.12% over the period 2014-2019 ... in non-traditional sectors is one of the major ...
(Date:2/19/2015)... , Feb .19, 2015 Research and ... the addition of the "Military Electro-Optical / ... Sensor Technology, by Platform - Forecast to 2020" ... The military electro-optical/infrared systems market is expected to ... CAGR of 7.71%. This report segments ...
Breaking Biology News(10 mins):World Iris Biometrics Industry 2World Iris Biometrics Industry 3World Iris Biometrics Industry 4World Iris Biometrics Industry 5World Iris Biometrics Industry 6World Iris Biometrics Industry 7World Iris Biometrics Industry 8World Iris Biometrics Industry 9World Iris Biometrics Industry 10World Iris Biometrics Industry 11Global 2D Gesture Recognition Market 2015-2019 with Cognitec Systems, CogniVue, eyeSight Mobile Technologies & PointGrab Dominating 2Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 2Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 3
... study calling for levels of obesity among South Asians to ... of Leicester. A team from the University,s Departments ... for the point at which South Asians should be classed ... published in PLoS One , a journal of the ...
... Management Institute ( http://cmi.vt.edu/ ), a research center ... Environment, has provided technical expertise for the world,s ... Forest Degradation (REDD) project to receive certification under ... ( http://www.v-c-s.org/ ). The UN-REDD Programme ( http://www.un-redd.org/ ...
... to scientists, carbon monoxide (CO), a tasteless, colorless and odorless ... also highly toxic to human beings. Found in the exhaust ... killer" because excessive inhalation is lethal, poisoning the nervous system ... Itzhak Schnell of Tel Aviv University,s Department of Geography and ...
Cached Biology News:New research calls for lower limit to be set for South Asian obesity level 2First UN carbon offset project certified 2Carbon monoxide -- the silent calmer? 2
... Sophions newly introduced QPatch HT is taking automated ... powerful level. With its ability to generate thousands ... is enabling new screening paradigms in ion channel ... with the same high quality afforded by conventional ...
... mouse embryonic stem cells to dopaminergic neurons. ... where they are mainly located in the ... the retrorubral field. These dopaminergic (DA) neurons ... ( e.g.projections to the caudate nucleus, putamen ...
... Microarrays designed for RNA aptamer screening and ... and powerful Paraflo microfluidic on-chip synthesis platform. ... be synthesized on-chip and LC Sciences can ... microarrays are available as part of our ...
... the C-terminal end of the mouse retinoic acid receptor beta (RAR*). ... of RAR*. The sequence of PEP-005 is (amino to carboxy terminus): ... S - S - V - E - N - S - ... L - L - Q (The N-terminal cysteine has been added ...
Biology Products: