Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B

nt amount of saturated pixels, select Total Density in Gel Image instead.

Compare the Cy3 and Cy5 images with the Scatter Plot view (Analyze>Scatter Plot). Activate and inactivate normalization to view the effect of normalization.

Multi-Channel Viewer
The multi-channel viewer (View>Multi-Channel Viewer) gives an initial visualization of matched and unique spots in the three Cy-labeled images, but it is not necessary for the analysis. Overlay the desired images and select the Auto-Scale Image When Assigned checkbox to adjust the images to similar brightness and contrast.

The following color scheme is suggested: blue for the Cy2 image, green for the Cy3 image, and red for the Cy5 image.

Analysis
Use Analysis Set Manager to create analysis sets that contain spots exhibiting significant protein regulation between the Cy3 and Cy5 samples (Analyze>Analysis Set Manager>Create). The Spot Review tool (Analyze>Spot Review) can be used to review these analysis sets and to verify that all the spots in each analysis set are defined as desired. A subset of these spots can be selected by clicking on their graphs and then selecting Create Analysis Set to establish the desired DIGE analysis set.

Preparative Gels
Carry out a separate spot detection on the SYPRO Ruby stained gel. Use the Automated Detection and Matching dialog box, and disable steps 3 and 4. It is not necessary for preparative gels to match perfectly to the corresponding DIGE gels in most cases the preparative and analytical gel images will appear very different. However, it should be possible to locate the proteins of interest that were targeted in the analytical gels in the
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:9/19/2014)... , Sept. 19, 2014 Pfenex Inc. (NYSE ... development of high-value and difficult to manufacture proteins including ... at the 21 st Annual NewsMakers in the ... . Bertrand Liang , chief executive officer of ... programs and business strategy on Friday, September 26, 2014 ...
(Date:9/19/2014)... (PRWEB) September 19, 2014 Cereal ... food industries will gather October 5–8, 2014, for ... Rhode Island Convention Center in Providence, Rhode Island. ... the work of food scientists, chemists, microbiologists, nutritionists, ... and food production management. To date, 1,000 attendees ...
(Date:9/19/2014)... The new , Healthcare and Life ... today on PharmaBoardroom.com for free download , ... the Spanish economy in 2009, and the austerity measures that ... pragmatic in times of trouble. "Despite the crisis, ... European market, and the general conditions to invest are favorable ...
(Date:9/18/2014)... About POCT POCT, ... clinical laboratory. It helps in making fast clinical ... medication. POCT is gaining popularity due to the ... as diabetes, heart disease, and obesity. It is ... to minimize errors during the diagnosis of patients. ...
Breaking Biology Technology:Pfenex to Present at Upcoming Industry Conference on September 26 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3PharmaBoardroom Releases New Spain Pharmaceuticals Report 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 3POCT Market in China 2014-2018 2
... - Third Wave Technologies, Inc ., has reported a ... thanks in part to higher clinical molecular diagnostic revenue during the ... quarter compared favorably to a net loss of $7.4 million, or ... stock compensation costs factored in, the company's third-quarter net loss improved ...
... Wis. - Virent Energy Systems, Inc. has ... Department of Agriculture and the Department of Energy ... of biodiesel production, into a renewable chemical called propylene glycol. ... USDA-DOE Biomass Research and Development Initiative , which was ...
... place in the telecom industry. Not an evolution - ... is being driven by technological advances that, in turn, drive ... in turn, create hyper-competition among long established and brand new ... with others in the value chain. , ,Consumers ...
Cached Biology Technology:Third Wave reports $5.2M third quarter loss 2Virent lands $2M grant for chemical production 2No time for evolution in telecommunications 2No time for evolution in telecommunications 3No time for evolution in telecommunications 4
(Date:9/18/2014)... to acquire and creatively manipulate spoken language is unique ... past 6 million years of human evolution to make ... best candidate gene we now have," says Wolfgang Enard, ... his efforts to understand the molecular biological basis of ... The results of his latest study, undertaken in collaboration ...
(Date:9/18/2014)... built the first smartphone app that automatically reveals ... In other words, your smartphone knows your state ... and how that affects you. , The StudentLife ... loneliness to their academic performance, also may be ... to monitor mental health, trigger intervention and improve ...
(Date:9/18/2014)... ALEXANDRIA, Va.Sept. 18, 2014Many Americans across racial and ... the greatest impact on their day-to-day life, more ... memory, hearing and speech (57% of African-Americans, 49% ... of Hispanics). When asked which disease or ailment ... blindness ranked first among African-Americans followed by AIDS/HIV. ...
Breaking Biology News(10 mins):Language evolution: Quicker on the uptake 2Language evolution: Quicker on the uptake 3New Dartmouth smartphone app reveals users' mental health, performance, behavior 2New Dartmouth smartphone app reveals users' mental health, performance, behavior 3New Dartmouth smartphone app reveals users' mental health, performance, behavior 4Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 3
... A team of researchers at the University of ... boardroom in an effort to build a business based ... The work of Claus Tittiger, professor of ... a highly competitive and intense National Science Foundation business-validation ...
... years of research at the Institute of Food Research, ... by food and drug companies worldwide. , IFR and ... secured funding from the Biotechnology and Biological Sciences Research ... to validate and improve how well it simulates the ...
... Pompeu Fabra University (Spain) have created a high resolution atlas ... The study demonstrates that an average image of an organ ... of comparing individual cases and differentiating healthy forms from pathologies. ... heart and its components such as the ventricles and ...
Cached Biology News:Insect research heads down path to start-up company with NSF I-Corps program 2Insect research heads down path to start-up company with NSF I-Corps program 3Commercial future for Model Gut 2An atlas of the human heart is drawn using statistics 2
... Immunogen: purified recombinant human ... coli . Physical form: ... filtered solution in phosphate buffered ... (c). WB. Titertest ...
CKB Antibody (N-term) Antigen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected within aa 1~100 of human CKB. Molecular Weight: 42644 Da...
...
The antibody recognizes the peptide sequence ESESQGPK (aa 441-448) of human class III beta-tubulin specific for neurones....
Biology Products: