Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B

nt amount of saturated pixels, select Total Density in Gel Image instead.

Compare the Cy3 and Cy5 images with the Scatter Plot view (Analyze>Scatter Plot). Activate and inactivate normalization to view the effect of normalization.

Multi-Channel Viewer
The multi-channel viewer (View>Multi-Channel Viewer) gives an initial visualization of matched and unique spots in the three Cy-labeled images, but it is not necessary for the analysis. Overlay the desired images and select the Auto-Scale Image When Assigned checkbox to adjust the images to similar brightness and contrast.

The following color scheme is suggested: blue for the Cy2 image, green for the Cy3 image, and red for the Cy5 image.

Analysis
Use Analysis Set Manager to create analysis sets that contain spots exhibiting significant protein regulation between the Cy3 and Cy5 samples (Analyze>Analysis Set Manager>Create). The Spot Review tool (Analyze>Spot Review) can be used to review these analysis sets and to verify that all the spots in each analysis set are defined as desired. A subset of these spots can be selected by clicking on their graphs and then selecting Create Analysis Set to establish the desired DIGE analysis set.

Preparative Gels
Carry out a separate spot detection on the SYPRO Ruby stained gel. Use the Automated Detection and Matching dialog box, and disable steps 3 and 4. It is not necessary for preparative gels to match perfectly to the corresponding DIGE gels in most cases the preparative and analytical gel images will appear very different. However, it should be possible to locate the proteins of interest that were targeted in the analytical gels in the
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:7/1/2015)... InferMed , s ... will augment ... s olutions suite   Elsevier , ... and services, announced today the acquisition of  InferMed , a ... company. InferMed,s Arezzo technology supports clinicians in choosing ...
(Date:7/1/2015)... 2015  UCB, a multinational biopharmaceutical company, and ... in designing, transforming, and running intelligent business operations, ... of their close collaboration in driving transformation that ... services (GBS) finance organization. The Aecus ... work together effectively to embrace the innovative promise ...
(Date:6/30/2015)... June 30, 2015 /PRNewswire/ - Resverlogix Corp. ("Resverlogix") ... shares of Resverlogix have approved the resolutions relating ... Limited of 5,600,000 units at a price of ... unit being comprised of one common share and ... of approximately CAD$15 million; and (ii) the amendment ...
(Date:6/30/2015)... 2015  Juniper Pharmaceuticals, Inc. (Nasdaq: JNP ), ... that address unmet medical needs in women,s health, ... will present at the Cantor Fitzgerald Inaugural Healthcare ... , 4:45 PM EDTLocation: , Le Parker Meridien ... www.juniperpharma.com, under  ,Investor, or  click here ...
Breaking Biology Technology:Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 2Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 3Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 2UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 5Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 2Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 3Juniper Pharmaceuticals to Present at Cantor Fitzgerald Inaugural Healthcare Conference on July 8, 2015 2
... Troy, N.Y. A new research discovery at Rensselaer ... frames for aircraft, watercraft, and automobiles. , Epoxy composites ... jets, planes, and other vehicles. Composite material frames are ... vehicle and boosts fuel efficiency. The downside is that ...
... NATURAL FUELS INDUSTRIES, INC. (Pink Sheets: VGPR) today announced ... the Company to "Fully Compliant", its highest tier for ... over-the-counter into easily identifiable tiers that inform investors whether ... a timely manner. Subsequent to NFI,s recent filing, ...
... ACF Manufacturing Drives Aggressive Conversion ProgramST. LOUIS, March 26 ... materials and services for upstream and downstream processes in ... a member of the Sigma-Aldrich Group (Nasdaq: ... of its dry powder cell culture media continuous milling ...
Cached Biology Technology:Fitter frames: Nanotubes boost structural integrity of composites 2Pink OTC Markets Moves Natural Fuels Industries to Top Tier 2SAFC Biosciences(TM) Completes Conversion of Lenexa Media Milling Facility to Animal Component Free 2SAFC Biosciences(TM) Completes Conversion of Lenexa Media Milling Facility to Animal Component Free 3SAFC Biosciences(TM) Completes Conversion of Lenexa Media Milling Facility to Animal Component Free 4
(Date:6/25/2015)... SALT LAKE CITY , June 25, 2015 /PRNewswire/ ... the first steps toward a healthier, happier life. That,s ... app is designed to do. The cutting-edge, portable health ... and offers real-life solutions to help improve your lifestyle ... Sellers Association awards dinner and gala Wednesday night, USANA,s ...
(Date:6/24/2015)... , June 24, 2015  Synaptics Inc. ... human interface solutions, today announced that Sharp has ... provide secure authentication for its latest flagship smartphone ... more than 200 million shipments of its fingerprint ... reinforces Synaptics, strength, scalability and leadership in the ...
(Date:6/23/2015)... 22, 2015 Research ... of the "Body-Worn Temperature Sensors Market - ... Forecast 2014 - 2020" report to their ... of the global body-worn temperature sensors market. The ... on the basis of types, care setting, patient ...
Breaking Biology News(10 mins):@USANAinc's Personalized Nutrition Program Wins Industry Innovation Award 2Synaptics Brings Natural ID Technology to Sharp's Newest Flagship Smartphone 2Body-Worn Temperature Sensors Market - Global Industry Analysis 2
... 19, 2012 Research!America Chair John Edward Porter says federal ... fiscal environment. "The rancorous budget debates between ... nation,s leadership in science and innovation is tenuous at best ... proposals in the House and Senate would put funding for ...
... The boundary between electronics and biology is blurring with ... Oak Ridge National Laboratory of ferroelectric properties in an ... team led by Andrei Kholkin of the University of ... to identify and explain the presence of ferroelectricity, a ...
... Barcelona, Spain, Thursday 19 April 2012: New data presented ... the interferon-free (IFN) revolution in HCV treatment. The much ... that combinations of antivirals offer the hope of shorter, ... following new studies cover the treatment of HCV patients ...
Cached Biology News:ORNL microscopy yields first proof of ferroelectricity in simplest amino acid 2New data suggests interferon-free therapy around the corner for HCV patients 2New data suggests interferon-free therapy around the corner for HCV patients 3