Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B

d scanning, set three of the channel tabs to conform to the different Cy dyes (e.g., set Channel 1 for Cy3, Channel 2 for Cy5, and Channel 3 for Cy2). The order does not matter. The Enable Channel box must be selected in each channel to automate the acquisition. A green check mark appears for each enabled channel.

Optimizing Imaging
To save time by avoiding scanning areas outside the gel, a quick initial scan at 800 m resolution should be done to find the location of the gel and the approximate intensity of the spots. Select the Highlight Saturated Pixels box to aid in interpreting sample intensity. No more than a few of the brightest spots should contain saturated pixels. If an image contains too many saturated pixels, reduce the photomultiplier tube (PMT) voltage. Change the PMT voltage through the Select Application pop-up window by selecting either a different preset sample intensity setting (e.g., high, medium, or low) or a custom voltage for more refined control. Keep in mind that high sample intensity (saturated pixels) requires a lower PMT voltage, and a low sample intensity requires a higher PMT voltage.

Preparative Gels and Protein Identification
For optimal mass spectrometry results, it is recommended that you use SYPRO Ruby stained preparative gels to obtain enough protein for identification.

Load the SYPRO Ruby stained gel into the Molecular Imager FX as described earlier. In the Molecular Imager FX acquisition window, select the SYPRO Ruby preset application and scan as before for the Cy-dyed gels.


Analysis
Image Analysis With PDQuest Software
PDQuest analyzes DIGE experiments with standard gel m
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:3/5/2015)... IDAHO (PRWEB) March 05, 2015 ... Ultralight Histology’s paraffin and stain additive. The improvement ... observe the binary features present in all daughter ... FFPE protocol, chromatin packaging was analyzed at 1000-1600x ... indicated that chromatin patterning in all daughter cells ...
(Date:3/5/2015)... Research and Markets ( http://www.researchandmarkets.com/research/4ld5nw/mature_biotech ) ... Outlook 2015: New Therapy Ventures Pave the Way for ... All biotech companies - Gilead (GILD), Amgen (AMGN), Biogen ... given a positive return and hence increased the interest ... select therapy/niche indication where the high treatment cost justifies ...
(Date:3/5/2015)... (PRWEB) March 05, 2015 ... acute and chronic orthopedic conditions such as meniscal ... tennis elbow, and joint pain due to degenerative ... administered during orthopedic surgeries to promote better post-surgical ... advances in nearly pain-free bone marrow harvesting with ...
(Date:3/4/2015)... March 4, 2015  Brooklyn residents now have a ... chronic pain and injuries. Nunzio Saulle , M.D., ... opening of his NJS Physical Medicine and Rehabilitation (PM&R) ... Thursday, March 5, 2015. Health Plus Management, LLC is ... located at 1178 Flatbush Avenue. This new PM&R practice ...
Breaking Biology Technology:Mature Biotech Outlook 2015: New Therapy Ventures Pave the Way for Success 2Mature Biotech Outlook 2015: New Therapy Ventures Pave the Way for Success 3Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 2Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 3Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 4Riordan-McKenna Institute of Regenerative Orthopedics and Stem Cell Therapy Announces Open House in Southlake, Texas on Friday, March 6th from 5:30 pm to 7:30 pm. 5NJS Physical Medicine & Rehabilitation Opening on Flatbush Avenue, Brooklyn, NY 2
... Sigma-Aldrich (Nasdaq:,SIAL) today announced the publication of its ... of Visualized Experiments (JoVE).,This makes Sigma-Aldrich the first ... the traditional scientific publication process. Peer-reviewed,video protocols are ... the,efficiency of scientific communication and with it the ...
... CRY ),a biomaterials, medical device and tissue processing company, ... upcoming UBS Global Life,Sciences Conference at the Grand Hyatt ... Lee, executive vice president, chief operating officer and,chief financial ... 2:30 p.m., Eastern Time., CryoLife,s live presentation may ...
... BAX ), will host a quarterly conference call to ... 16, 2008, at,8:30 a.m. Eastern Time (7:30 a.m. Central ... or 703-639-1156 (international). Please dial,into the call at least ... for the,operator to connect you., This call is ...
Cached Biology Technology:Sigma-Aldrich(R) Becomes First Life Science Company to Publish in PubMed's Peer-Reviewed Video Journal JoVE 2Sigma-Aldrich(R) Becomes First Life Science Company to Publish in PubMed's Peer-Reviewed Video Journal JoVE 3CryoLife to Present at UBS Global Life Sciences Conference 2Baxter International Third Quarter 2008 Financial Results Conference Call 2
(Date:1/22/2015)... Jan. 13, 2015 /PRNewswire/ - Today, FindBiometrics, the leading source of ... of its new website design. "When we launched ... says Peter O,Neill , founder and CEO of FindBiometrics. ... needs involvement from the key players on a very broad ...
(Date:1/22/2015)... Hall E -   EyeLock Inc. , a market leader ... its EyeLock ID technology integrated in a 3D printed automobile ... Ridge National Laboratory (ORNL) at the 2015 North ... is being used to validate the driver and authorize the ...
(Date:1/22/2015)... Jan. 16, 2015  A man-made form of insulin delivered ... mental capabilities in adults with mild cognitive impairment and ... by researchers at Wake Forest Baptist Medical Center. ... amnesic mild cognitive impairment (MCI) or mild to moderate ...
Breaking Biology News(10 mins):Identity Industry Leading Publication Debuts New Look As Biometrics Hit Mainstream 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3
... 13, 2008.-Unlike most scientific and technological advances, which tend ... finds itself the center of public debate and regulatory ... many of its applications. , In this second ... first was in 2003), the sample has been enlarged ...
... for humans in medical research, thanks to genomes that ... differently in mouse and man, a study by University ... reveals. Their results, which have implications for the ... in the current issue of the journal Proceedings of ...
... Md. (May 14, 2008) A study investigating aging in mice ... significantly less may help explain an already established phenomenon: a ... benefit that even regular exercise does not achieve. We ... many diseases, but key rodent studies tell us that being ...
Cached Biology News:BBVA Foundation international study on attitudes to stem cell research 2BBVA Foundation international study on attitudes to stem cell research 3BBVA Foundation international study on attitudes to stem cell research 4BBVA Foundation international study on attitudes to stem cell research 5BBVA Foundation international study on attitudes to stem cell research 6Mouse can do without man's most treasured genes 2Mouse can do without man's most treasured genes 3Mouse study: When it comes to living longer, it's better to go hungry than go running 2Mouse study: When it comes to living longer, it's better to go hungry than go running 3Mouse study: When it comes to living longer, it's better to go hungry than go running 4
... Signet offers the most comprehensive, ... requirements. We have incorporated unprecedented quality ... capabilities. This includes proven detection chemistries ... to the TechMate protocol. It also ...
Functionally tested for consistent G-banding of chromosomes....
... Cell Medium is optimized for serum-free growth ... In this protein-free medium, Sf9 cultures routinely ... cells/ml with viabilities above 95%. This medium ... color from X-gluc staining of pBACgus recombinants ...
... Insect Medium is formuatled for the ... Sf9 and Sf21 cells. Grace's Insect ... because it does not contain lactalbumin ... with transfection. Grace's Insect Medium, Supplemented ...
Biology Products: