Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B

r Imager FX System
Since proteins are covalently labeled with the Cy dyes, post-run staining is not necessary for analysis. Bio-Rads Ready Gel, Criterion, and PROTEAN II precast gels must be removed from their cassettes and placed on the standard glass sample tray. Gels in glass cassettes may be scanned while in the cassettes as long as the glass has low fluorescence and the plates are not thicker than 11.5 mm. Removing at least the top glass plate prior to scanning is recommended. Scans obtained through top glass plates are at least 2-fold less intense than those obtained with the plates removed. Gels removed from their cassettes should be placed on the standard glass sample tray. For gels between thick glass plates, the multi-sample tray II should be used with an appropriate spacer to bring the sample into the proper focal plane for imaging.

Setting Up the Imaging Software
The Molecular Imager FX acquisition screen contains preset applications to automatically select the correct laser and filter combinations. Select Cy2, Cy3, and Cy5 from the preset applications located in the PDQuest acquisition window.

Automated Scanning
Once the applications are created, images can be acquired sequentially or automatically. Make sure that the acquisition frame is tightly wrapped around the gel image, and do not change the frame between successive scans. Since all three images are obtained from the same gel, it is essential that the gel not be moved between scans and that the acquisition frame be the same for all scans.

For sequential scanning, simply select the Cy3 setting in Channel 1 and scan. Repeat for Cy5 and Cy2. For automate
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:9/22/2014)... 2014 Follow us on ... young has groomed and nurtured anti-aging products and ... emphasis on appearance maintenance is benefitting the market ... protein obtained from the bacterium, Clostridium Botulinum, which ... relaxation. With cosmetic indications for botulinum toxin ranging ...
(Date:9/22/2014)... 22, 2014 FierceBiotech today announced its ... biotechnology companies of 2014. FierceBiotech editors John Carroll ... this year’s winners. Those selected are identified as ... the industry. , "It's always a lot ... Fierce 15 companies," said Carroll. "This year in particular ...
(Date:9/22/2014)... Sept. 22, 2014  Spherix Incorporated (SPEX) -- an intellectual ... intellectual property, today announced that the United States Patent & ... in the month of September that is part of a ... patent is: USRE45,121 , Application number: US 13/728,867 ... date: Dec 27, 2012 The listed inventors ...
(Date:9/20/2014)... 2014 HealthTronics, Inc. , ... radiology products and services, has signed a distribution ... Clinicon Corporation for its high precision laser ... minimally invasive, mobile, surgical technology—offering more choices for ... agreement, HealthTronics will make available Clinicon’s FDA-cleared SureLase™ ...
Breaking Biology Technology:Strong Demand for Non-Invasive Cosmetic Procedures Drives the Global Botulinum Toxin Market, According to New Report by Global Industry Analysts, Inc. 2Strong Demand for Non-Invasive Cosmetic Procedures Drives the Global Botulinum Toxin Market, According to New Report by Global Industry Analysts, Inc. 3FierceBiotech Announces Annual “Fierce 15” Winners of 2014 2Spherix Notified by United States Patent & Trademark Office of New Standard Essential Patent Issued to Spherix 2Spherix Notified by United States Patent & Trademark Office of New Standard Essential Patent Issued to Spherix 3HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 2HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 3
... on Thursday announced that three Wisconsin businesses would receive ... Commerce Technology Assistance Grant (TAG) and Technology Venture Fund ... , Functional Biosciences, Inc. , is a ... It will receive a $250,000 TVF loan for work ...
... ads on their round, shiny stomachs as part of "tummy ... To some, this is "desperate branding" in action. , ... spot on the list of branding failures. These projects are ... weird processes. , ,Roy Disney said, "You need branding when ...
... summer. Business slows. Indeed, in Europe, where I find myself ... animation that will last until the first of September. People ... grasp weakens. , ,Or does it? , ,After a rare ... mountain in my inbox and a to-do list that had ...
Cached Biology Technology:A final word on branding 2A final word on branding 3A final word on branding 4A final word on branding 5Leaving it all behind 2
(Date:9/21/2014)... the University of California, San Diego, have developed a ... control the activity of genes based on sequences of ... its application to human embryonic cells in a paper ... , "All of our cells have the same blueprint, ... John Whitaker, lead author of the report. "Skin cells ...
(Date:9/21/2014)... -- Shellfish such as mussels and barnacles secrete very ... ship hulls, even underwater. Inspired by these natural adhesives, ... that could be used to repair ships or help ... new waterproof adhesives, the MIT researchers engineered bacteria to ... proteins as well as a bacterial protein found in ...
(Date:9/19/2014)... today by researchers from several Harvard University labs ... experienced and aspiring researchers with the intellectual raw ... made from soft, flexible materials. , With the ... other advances in manufacturing technology, soft robotics is ... drawn from conventional rigid robot design, but working ...
Breaking Biology News(10 mins):Program predicts placement of chemical tags that control gene activity 2Engineered proteins stick like glue -- even in water 2Engineered proteins stick like glue -- even in water 3Soft robotics 'toolkit' features everything a robot-maker needs 2
... Not only land birds, but also some seabirds, ... example, circumnavigates the earth one and a half times ... about the migratory behaviour of seabirds as compared with ... Planck Institute for Ornithology have studied the migratory behaviour ...
... In a paradigm changing discovery, Plasmodium vivax ( ... historically thought to be resistant to the disease, those who ... red blood cells, according to researchers from Case Western Reserve ... of Health. In a study of more than 600 individuals ...
... experimental oral drug has lowered blood sugar levels and inflammation ... could someday be added to the arsenal of drugs used ... research. The drug consists of a synthetic molecule that ... inhibitory factor, or MIF. This protein is implicated in a ...
Cached Biology News:To the Antarctic or Brazil for new feathers 2To the Antarctic or Brazil for new feathers 3Duffy-negative blood types no longer protected from P. Vivax malaria 2Duffy-negative blood types no longer protected from P. Vivax malaria 3Study points to potential new drug for type 2 diabetes 2Study points to potential new drug for type 2 diabetes 3Study points to potential new drug for type 2 diabetes 4
... The Holten MaxiSafe 2010 Class II ... ergonomical working condition of comfort while ... efficiency and performance.,Refined operator, environment and ... both exhausted and recirculated air.,Bottom filters ...
... Jouan MSC series of Class II ... conditions and certification to the world's ... ensures that the cabinets cannot be ... Natural lighting, low noise and arm ...
... Stand-96 is designed for paramagnetic bead precipitation ... ml PCR plates with no additional accessories. ... in a standard SBS 96-well microplate footprint. ... magnets guarantees easy and fast (as little ...
... circular clear acrylic chamber for complete visibility ... features microprocessor operation for precise mixing capability. ... Carousel rotates at 11 rpm; manufactured ... , Bottle Capacity - four 35 ...
Biology Products: