Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B


Difference gel electrophoresis (DIGE) is a relatively straightforward application of differential labeling of protein samples using fluorescent dyes. The technique, originally published by Unlu et al. (1997), uses cyanine (Cy) dyes to differentially label proteins separated by 2-D gel electrophoresis. Following labeling and electrophoresis, excellent results can be achieved by scanning the multiplex gels with the Molecular Imager FX multiimager system and analyzing them with PDQuest 2-D analysis software.

The DIGE method is designed to compare two complex protein samples derived from two different growth conditions. One sample is labeled with Cy3 and the other with Cy5. A third dye, Cy2, is used to label a 50:50 mixture of the two samples, which becomes a standard for registering the images obtained with the other two dyes. Equal amounts of the three labeled protein mixtures are combined and run on the same 2-D polyacrylamide gel, eliminating any gel-to-gel variation.


Imaging
Image Acquisition With the Molecular Imager FX System
The Molecular Imager FX multiimager systems are well suited for image acquisition from gels containing Cy-dyed proteins. The laser and filter combinations provided ensure minimal cross-talk between the different fluors.

Lasers
The lasers required for the Cy dyes are the 488 nm external laser for Cy2, the 532 nm internal laser for Cy3, and the 635 nm external laser for Cy5.

Emission Filters
The required emission filters are 530BP30 for Cy2, 605DF50 for Cy3, and 695DF55 for Cy5.

Loading the Gels Into the Molecula
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:9/19/2014)... September 19, 2014 ... http://www.researchandmarkets.com/research/b2jxhs/micro_market ) has announced the addition of the  ...  report to their offering.       (Logo: ... one of the fastest-growing segments in the chromatography ... in 2013, and expected to reach $2.0 billion ...
(Date:9/18/2014)... TX (PRWEB) September 18, 2014 ... integrated urological and interventional radiology products and services, ... known as cryotherapy and cryoablation) versus external beam ... Section of the American Urological Association (MAAUA) Annual ... , "Comparing Relative Effectiveness of Cryosurgery and External ...
(Date:9/18/2014)... Sept. 18, 2014  Health Enhancement Products, Inc. (OTCQB: HEPI), ... algae bioactive compounds and metabolic processes, is pleased to announce ... of directors. Ms. Nola E. Masterson , a ... the board effective September 17, 2014. "We,re ... join our board," states Andrew Dahl , President and ...
(Date:9/18/2014)... Research and Markets has announced the addition ... Report, 2014-2017" report to their offering. ... differentiate into specialized cells and can divide (through mitosis) ... be applied to treatment of cardiovascular diseases, leukemia (a ... or lesion of liver, kidney and other parenchymal organs, ...
Breaking Biology Technology:Micro Market Monitor : Global Pre-packed Chromatography Columns 2HealthTronics Announces Data Comparing Cryotherapy Versus Radiation for Treating Prostate Cancer Poster Presented at the American Urological Association Regional Meeting 2Health Enhancement Announces New Board Member In Advance Of Annual Shareholder Meeting 2Global and Chinese Stem Cell Industry Report, 2014-2017 2Global and Chinese Stem Cell Industry Report, 2014-2017 3
... Should the periodic table bear a warning label in the ... supply and demand? If so, that lesson could start ... growing endangered, like the Asiatic dhole phosphorus. Why ... plants and animals. It is a key ingredient in fertilizer, ...
... Sangamo BioSciences, Inc. (Nasdaq: SGMO ) announced today ... update on the progress of Sangamo,s ZFP Therapeutic® development programs ... am ET on Friday, October 21, 2011 at the 18th ... held in New York City. The presentation will ...
... Research Council (BBSRC), among others, have taken the first step ... against coccidiosis, the most important parasite of poultry globally. ... from the coccidiosis bug rather than being derived from a ... than is currently possible so could be used to provide ...
Cached Biology Technology:Endangered species? Should cheap phosphorus be first on an elemental 'Red List?' 2Endangered species? Should cheap phosphorus be first on an elemental 'Red List?' 3Endangered species? Should cheap phosphorus be first on an elemental 'Red List?' 4Sangamo BioSciences Announces Presentation at the 18th Annual Newsmakers in the Biotech Industry Conference 2A step towards new vaccines for most important chicken parasite 2A step towards new vaccines for most important chicken parasite 3
(Date:9/18/2014)... a world-leading provider of scientific, technical and medical ... Management Society (ARMS), recognized the academic research achievements ... Young Researcher of the Year Awards. , The ... Convention Centre and in conjunction with the ARMS ... Australia,s and New Zealand,s scientific community. Guest of ...
(Date:9/18/2014)... the ultimate form of camouflage: you don,t just blend ... is not as uncommon as you might think. Kathryn ... explains that the larval life stages of many marine ... the anatomy that most creatures cannot make transparent. Feller ... shield each individual eye unit with an opaque pigment ...
(Date:9/17/2014)... and affordable health care to reproductive technologies, the ... the ability of people to identify key issues, ... respectfully, and find the most defensible ways forward. ... support these societal conversations?, The Hastings Center and ... Issues have teamed up to publish a series ...
Breaking Biology News(10 mins):Elsevier and Australasian Research Management Society award scientists at Scopus ceremony 2Transparent larvae hide opaque eyes behind reflections 2Why bioethics literacy matters 2Why bioethics literacy matters 3
... probes those that can see inside single living cells ... areas of the body. NewYork-Presbyterian Hospital/Weill Cornell Medical ... the narrow bile ducts that connect the liver to the ... device to minutely explore the pancreatic duct as one of ...
... adopt the first free textbooks from upstart publisher OpenStax ... save students at least $1 million this fall after ... or downloaded in their first 10 weeks on the ... more money in one semester than they cost to ...
... Biofuels and Texas AgriLife Research, part of The Texas ... develop and commercialize cellulosic feedstocks for the production of ... diverse high biomass energy crop breeding program and BP ... companies growing commercial-scale biomass crops for liquid fuels. ...
Cached Biology News:Deep inside the body, tiny mechanical microscope 2Deep inside the body, tiny mechanical microscope 3OpenStax College's free textbooks will save students $1 million this fall 2BP Biofuels, Texas AgriLife Research sign agreement to advance biofuel feedstock development 2
...
... versatile enclosures are designed ... or other process gas ... environment. HEPA filtration, heating/cooling, ... modules also available, along ...
... The new Hummingbird has the ability to quickly ... plate makes it a perfect candidate for inclusion ... simple OCX interface supplied with all the Cartesian ... store. Hummingbirds ability to copy plates in ...
Sigmas E-TOXATE (Limulus Amebocyte Lysate) test kits are intended for the detection and semiquantitation of endotoxins for research purposes. sensitivity 0.05-0.1 EU/mL...
Biology Products: