Navigation Links
Imaging and Analysis of DIGE Product Information Sheet, Rev B


Difference gel electrophoresis (DIGE) is a relatively straightforward application of differential labeling of protein samples using fluorescent dyes. The technique, originally published by Unlu et al. (1997), uses cyanine (Cy) dyes to differentially label proteins separated by 2-D gel electrophoresis. Following labeling and electrophoresis, excellent results can be achieved by scanning the multiplex gels with the Molecular Imager FX multiimager system and analyzing them with PDQuest 2-D analysis software.

The DIGE method is designed to compare two complex protein samples derived from two different growth conditions. One sample is labeled with Cy3 and the other with Cy5. A third dye, Cy2, is used to label a 50:50 mixture of the two samples, which becomes a standard for registering the images obtained with the other two dyes. Equal amounts of the three labeled protein mixtures are combined and run on the same 2-D polyacrylamide gel, eliminating any gel-to-gel variation.


Imaging
Image Acquisition With the Molecular Imager FX System
The Molecular Imager FX multiimager systems are well suited for image acquisition from gels containing Cy-dyed proteins. The laser and filter combinations provided ensure minimal cross-talk between the different fluors.

Lasers
The lasers required for the Cy dyes are the 488 nm external laser for Cy2, the 532 nm internal laser for Cy3, and the 635 nm external laser for Cy5.

Emission Filters
The required emission filters are 530BP30 for Cy2, 605DF50 for Cy3, and 695DF55 for Cy5.

Loading the Gels Into the Molecula
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
3. Imaging Chemiluminescent Western Blots
4. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
5. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
6. Performance of the AMARA HitHunter Chemiluminescence Kinase Assay with the LEADseeker Multimodality Imaging System
7. The development of a melanocortin MC4 receptor binding assay using the LEADseeker Multimodality Imaging System
8. The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System
9. The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System
10. Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells
11. Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Imaging and Analysis DIGE Product Information Sheet Rev

(Date:11/26/2014)... 2014 Whitehouse Laboratories is pleased ... Morris County (New Jersey) Chamber of Commerce as ... business organization in the North Jersey area, offers ... some key clients in the pharmaceutical and biotechnology ... area. Membership enables Whitehouse Laboratories a higher level ...
(Date:11/26/2014)... (PRWEB) November 25, 2014 Continuing its ... products, earned three prestigious MarCom Awards for outstanding creative ... received a Platinum MarCom Award, the organization’s top honor, ... in St. Louis held in April of this year. ... than 14,000 attendees. Additionally, Nerium was awarded a Gold ...
(Date:11/26/2014)... West Chester, OH (PRWEB) November 25, 2014 ... International Holdings, has joined the Graphel Carbon Products team ... experience in sales, marketing, and customer service for the ... to add Miles to our team,” stated Dave Trinkley, ... Products. “His wide range of experience in the ...
(Date:11/26/2014)... 2014 Deep Research Report on ... report on the Global Palmitic Acid industry. For ... basic information, including definition, classification, application, industry chain ... etc. , For international and China market ... in China and other countries or regions ...
Breaking Biology Technology:Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 2Miles Holder Joins Graphel Corporation as Sales/Marketing Manager 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 3
... EORTC-NCI-AACR Symposium -, SOUTH SAN FRANCISCO, ... Pharmaceuticals, Inc. (Nasdaq: PARD ), a ... incremental data from,its ongoing Phase 2 clinical ... prednisone as first-line therapy for metastatic,hormone-refractory prostate ...
... National Institutes of Health, SAN DIEGO, Oct. ... of a $28 million contract from the National ... of the National,Institutes of Health, for the development ... Under the five-year,contract, Trius will develop novel drugs ...
... Cadence Pharmaceuticals,Inc. (Nasdaq: CADX ), a biopharmaceutical ... candidates principally,for use in the hospital setting, announced ... will present the company,s corporate,overview on Tuesday, October ... during the BIOCOM Investor Conference at the Hyatt ...
Cached Biology Technology:Poniard Announces Positive Incremental Efficacy and Safety Data From Ongoing Phase 2 Picoplatin Trial in Hormone-Refractory Prostate Cancer 2Poniard Announces Positive Incremental Efficacy and Safety Data From Ongoing Phase 2 Picoplatin Trial in Hormone-Refractory Prostate Cancer 3Poniard Announces Positive Incremental Efficacy and Safety Data From Ongoing Phase 2 Picoplatin Trial in Hormone-Refractory Prostate Cancer 4Poniard Announces Positive Incremental Efficacy and Safety Data From Ongoing Phase 2 Picoplatin Trial in Hormone-Refractory Prostate Cancer 5Trius Awarded $28 Million Contract to Develop Novel Antibiotics 2Cadence Pharmaceuticals' CEO Theodore Schroeder to Present at the BIOCOM Investor Conference 2
(Date:11/12/2014)... Nov. 12, 2014 Crossmatch™, a leading provider ... U.are.U ® fingerprint readers have been deployed throughout ... Central Mexico . The bakery chain implemented ... payroll issues caused by employees clocking in for each ... fingerprint readers, Montparnasse relied on paper timecards and a ...
(Date:11/7/2014)... Florida , November 6, 2014 ... authentication for better mobile security revolutionizing online transactions.  Companies in ... Alibaba Holdings Ltd. (NYSE: BABA ), Google Inc. ... MSFT ), eBay Inc. (NASDAQ: EBAY ) and ... NXTD and NXTDW), a biometric authentication company focused ...
(Date:11/5/2014)... of biology in the UTSA College of Sciences, ... selected to receive a two-year $300,00 National Science ... (EAGER). The funding supports President Obama,s BRAIN Initiative, ... new technology that will demystify complex brain processes. ... neuroscience are broken into the interactions of multiple ...
Breaking Biology News(10 mins):Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6UTSA biology professor awarded $300,000 NSF grant for brain research 2UTSA biology professor awarded $300,000 NSF grant for brain research 3
... international collaboration effort in Mongolia to be used in ... collected from wild birds in Mongolia by field veterinarians ... have been selected by the World Health Organization to ... currently in development. The samples, collected in ...
... of the body's primary strategies for regulating its genome ... small molecules called microRNAs, or miRNAs. First observed only ... messenger RNA, itself responsible for translating genes into proteins. ... clearly important players on the genetic field despite having ...
... a study in Botswana, Harvard School of Public Health ... in resource-poor settings, who receive antiretroviral (ARV) therapy, can ... A fully supported health care delivery system and infrastructure ... November 2005 issue of the Journal of Acquired Immune ...
Cached Biology News:Wild birds help to create human flu vaccine 2Wild birds help to create human flu vaccine 3Twin molecular scissors link creation of microRNAs with gene-silencing 2Largest computational biology simulation mimics life’s most essential nanomachine 2
... DNA Polymerase is ideal for high-fidelity ... applications such as cloning and mutagenesis. ... a proprietary enzyme preparation containing recombinant ... proofreading (3'Cut Site5' exonuclease) activity. ...
... BV-10 offers precision beveling of ... 50m. The unique abrasive plate ... for greater control of the ... accomplished very rapidly and produces ...
... 2 mM sodium phosphate buffer, pH 6.5. ... is a major lysosomal aspartyl protease in ... proenzyme. Overexpression of cathepsin D in human ... of relapse and metastasis. Cathepsin D degrades ...
Rat Hepatocytes (RH) (>3,000,000 cells)...
Biology Products: