Navigation Links
Highly Active and Removable AffinityTagged Lambda Phosphatase

Dualspecificity Lambda Phosphatase useful for dephosphorylation serine, threonine or tyrosine residues

Danny Q. Hoang * Mary Ellen Simcox
Stratagene Cloning Systems, Inc.

Stratagene's recombinant Lambda Phosphatase is a dualspecificity protein phosphatase that has been produced as a fusion to Stratagene's novel affinity tag, the calmodulinbinding peptide. The tag simplifies purification of the enzyme and allows the enzyme to be removed subsequent to in vitro dephosphorylation of substrate proteins. The activity of the target protein can then be assayed in the absence of phosphatase activity. This feature is an essential advantage when the substrate protein itself is a protein phosphatase or protein kinase.

Protein phosphorylation governs many biological processes, including metabolism, gene expression, cytoskeletal architecture, cell adhesion and the cell cycle.1 The level of protein phosphorylation in cells is modulated by two large families of enzymes, the protein kinases and the protein phosphatases. Kinases and phosphatases are classified according to their substrate specificities as tyrosine, serine/ threonine or dualspecificity enzymes.2,3

Lambda phosphatase is a dualspecificity phosphatase4 that is useful for dephosphorylating proteins phosphorylated on serine, threonine or tyrosine residues. The enzyme can be used to determine whether a protein of interest is phosphorylated by analyzing the protein by SDSPAGE, plus and minus phosphatase treatment. Dephosphorylation results in a significant shift in electrophoretic mobility. In addition to this general application, Stratagene's novel Lambda Phosphatase is readily removable; binding to calmodulin affinity resin completely eliminates lambda phosphatase activity. By using Stratagene's Lambda Phosphatase, investigators are able to assay the activity of a dephosphoryl
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
2. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
3. Highly Sensitive microRNA Array Performance
4. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
5. Production of Full-Length, Biologically Active MEK1WT and MEK1CA
6. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
7. Translate High Yields of Active Protein
8. Translate High Yields of Biologically Active Proteins
9. A New Lambda Vector for Mammalian Expression
10. Lambda DNA Extraction Protocol
11. Lambda DNA Extraction Protocol
Post Your Comments:
(Date:3/26/2015)... SAN DIEGO , March 26, 2015  BioNano ... the appointment of  Terry Salyer as chief commercial ... building highly effective, worldwide sales teams in life sciences. ... Irys ® System, a genome-mapping platform to assemble ... and investigate structural variations. To date, 32 institutions located ...
(Date:3/26/2015)... March 26, 2015 "In America," the popular ... United States that is hosted by legendary film actor James ... of botany in an upcoming segment. , Botany is ... reality it is actually quite a bit more complicated than ... different types of living organisms, from the smallest bacteria known ...
(Date:3/25/2015)... 2015 /PRNewswire/ - SQI Diagnostics Inc. ("SQI" or the "Company") ... of recent discussions with certain shareholders representing approximately 45% ... after dealing with certain matters including the appointment of ... on March 26, 2015 (the "Meeting") it intends to ... will take place when the Meeting reconvenes. The Meeting ...
(Date:3/25/2015)... March 25, 2015 Accelovance, a therapeutically focused ... for "Best Contract Research Organization" at the upcoming Vaccine ... Congress. This is the eighth consecutive ... the award, winning for "Best Contract Research Organization" in ... distinction in 2013. "We,re excited to be ...
Breaking Biology Technology:BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 2BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 3SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 2SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 3SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 4SQI Diagnostics Inc. Announces New Nominees to SQI Diagnostics Board and Adjournment of Meeting 5Accelovance Nominated for 2015 Vaccine Industry Excellence (ViE) Awards 2
... NEW BRUNSWICK, N.J., Dec. 5 Senesco Technologies, Inc. ... today that its Development and License Agreement with the ... mutual agreement because of Harris Moran,s recently announced corporate ... Harris Moran has reported that its parent company, Limagrain, ...
... BioCryst Pharmaceuticals (Nasdaq: BCRX ) announced today ... and BCX-4208, and clinical data on BCX-4208 at the 50th ... held in San Francisco, CA from December 6-9, 2008. ... inhibitors. , , The schedule ...
... YM BioSciences Inc. (NYSE Alternext US:YMI, TSX:YM, ... and commercializes differentiated products for patients worldwide, ... licensee of nimotuzumab from YM,s subsidiary, CIMYM ... the European Medicines Agency (EMEA) for marketing ...
Cached Biology Technology:Senesco and Harris Moran Mutually Agree to Terminate Development and License Agreement Due to Restructuring 2Senesco and Harris Moran Mutually Agree to Terminate Development and License Agreement Due to Restructuring 3BioCryst to Present Three Posters During the 50th American Society of Hematology Annual Meeting and Exposition 2BioCryst to Present Three Posters During the 50th American Society of Hematology Annual Meeting and Exposition 3YM BIOSCIENCES PROVIDES EU REGULATORY UPDATE 2YM BIOSCIENCES PROVIDES EU REGULATORY UPDATE 3
(Date:3/11/2015)... 11, 2015 Research and Markets ( ... "Access Control Market by Product, Application & By Geography ... to their offering. , This report predicts ... $10.4 billion by 2020, with an estimated CAGR of ... the products types such as contact cards & readers, ...
(Date:3/10/2015)...  NXT-ID, Inc. (NASDAQ: NXTD ... authentication company focused on the growing mobile commerce ... been named the "Number One Best Tech Gift ... Modern ( http://www.rethinkmodern.com/ ) is a web magazine focused ... that have a unique function or design.  ...
(Date:3/2/2015)... 2015  Businesses have a new option for ... Rankins Enterprises has announced the launch of a ... for businesses to protect their customers, personal data, ... Beconux is a biometric transaction processing system ... to an ATM machine, the new system fuses ...
Breaking Biology News(10 mins):Global Access Control (Cards & Readers, Biometrics, Controllers, Management Software) Market 2015-2020 - Analysis of the $10 Billion Market 2NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 2NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 3NXT-ID's Wocket Smart Wallet Named Number One Best Tech Gift for 2015 by Rethink Modern 4Personal Data Protection Company Launches New Product 2
... of California, Berkeley, virologist and an assistant professor in the ... M. Keck Foundation grant, an award given to innovative young ... which totals up to $1 million over five years, will ... herpesvirus as a tool to study the inner workings of ...
... WORCESTER, Mass.In recent years, a class of small molecules known ... in regulating gene products in most animal and plant species. ... development of alcohol tolerance, a hallmark of alcohol abuse and ... and Alcoholism (NIAAA) report the findings in the July 31 ...
... death but now a study of brain tissue collected during ... and suicide. The international research group, led by Dr. ... of Western Ontario and Dr. Hymie Anisman of the Neuroscience ... that proteins that modify DNA directly are more highly expressed ...
Cached Biology News:UC Berkeley virologist named Keck Distinguished Young Scholar 2MicroRNA implicated as molecular factor in alcohol tolerance 2Study identifies changes to DNA in major depression and suicide 2
Immunogen: Synthetic peptide (KLH conjugated): L(506) S K S S S Q I R M S G L P A D N L A T(525) Storage: -20 C, Avoid Freeze/Thaw Cycles...
...
This Universal 24" X 18" (61 X 45.7cm.) shaker platforms will accept any Thermo Forma shaker flask clip....
Disposable tips with floating filter (US Patent 6,702,990) for PDM1.5 OneTouch Plus spotpicker, 10 racks of 96, DNAse and RNAse free...
Biology Products: