Navigation Links
Highly Active and Removable AffinityTagged Lambda Phosphatase

Dualspecificity Lambda Phosphatase useful for dephosphorylation serine, threonine or tyrosine residues

Danny Q. Hoang * Mary Ellen Simcox
Stratagene Cloning Systems, Inc.

Stratagene's recombinant Lambda Phosphatase is a dualspecificity protein phosphatase that has been produced as a fusion to Stratagene's novel affinity tag, the calmodulinbinding peptide. The tag simplifies purification of the enzyme and allows the enzyme to be removed subsequent to in vitro dephosphorylation of substrate proteins. The activity of the target protein can then be assayed in the absence of phosphatase activity. This feature is an essential advantage when the substrate protein itself is a protein phosphatase or protein kinase.

Protein phosphorylation governs many biological processes, including metabolism, gene expression, cytoskeletal architecture, cell adhesion and the cell cycle.1 The level of protein phosphorylation in cells is modulated by two large families of enzymes, the protein kinases and the protein phosphatases. Kinases and phosphatases are classified according to their substrate specificities as tyrosine, serine/ threonine or dualspecificity enzymes.2,3

Lambda phosphatase is a dualspecificity phosphatase4 that is useful for dephosphorylating proteins phosphorylated on serine, threonine or tyrosine residues. The enzyme can be used to determine whether a protein of interest is phosphorylated by analyzing the protein by SDSPAGE, plus and minus phosphatase treatment. Dephosphorylation results in a significant shift in electrophoretic mobility. In addition to this general application, Stratagene's novel Lambda Phosphatase is readily removable; binding to calmodulin affinity resin completely eliminates lambda phosphatase activity. By using Stratagene's Lambda Phosphatase, investigators are able to assay the activity of a dephosphoryl
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
2. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
3. Highly Sensitive microRNA Array Performance
4. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
5. Production of Full-Length, Biologically Active MEK1WT and MEK1CA
6. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
7. Translate High Yields of Active Protein
8. Translate High Yields of Biologically Active Proteins
9. A New Lambda Vector for Mammalian Expression
10. Lambda DNA Extraction Protocol
11. Lambda DNA Extraction Protocol
Post Your Comments:
(Date:7/24/2014)... July 24, 2014   Cypher Genomics, Inc., ... appointment of Adam Simpson as president ... rapid and comprehensive annotation and interpretation of individual ... "Cypher,s technology has the potential to ... enhancing diagnostic accuracy, optimizing therapeutic approaches and reducing ...
(Date:7/23/2014)... NEW YORK , July 24, 2014 /PRNewswire/ ... and Immune Pharmaceuticals Inc. (OTCQX and NASDAQ OMX ... "IMNP."; "Immune" or "the Company"), announced a collaboration ... bullous pemphigoid last night at an event attended ... other interested parties. Lyfebulb is a health and ...
(Date:7/23/2014)... efforts have made great strides in saving China,s endangered ... are over 65 giant panda reserves that have been ... efforts, just 1596 pandas remain in the wild. ... to save the panda by improving genetic diversity, avoid ... Just how are these high-profile programs doing so far? ...
(Date:7/23/2014)... , July 23, 2014 /PRNewswire-iReach/ -- Think Drinks ... performance for the long term. They are the first ... of sustained energy as an alternative to the temporary ... Drinks on Indiegogo on July 29, 2014, with hopes ... campaign. Photo - http://photos.prnewswire.com/prnh/20140723/129697 ...
Breaking Biology Technology:Cypher Genomics Announces the Appointment of Adam Simpson as President and COO to Advance Transformative Genome Interpretation Technology 2Cypher Genomics Announces the Appointment of Adam Simpson as President and COO to Advance Transformative Genome Interpretation Technology 3Innovative Collaboration To Increase Awareness Of Bullous Pemphigoid, A Rare Autoimmune Disease Of The Skin 2Innovative Collaboration To Increase Awareness Of Bullous Pemphigoid, A Rare Autoimmune Disease Of The Skin 3Innovative Collaboration To Increase Awareness Of Bullous Pemphigoid, A Rare Autoimmune Disease Of The Skin 4Innovative Collaboration To Increase Awareness Of Bullous Pemphigoid, A Rare Autoimmune Disease Of The Skin 5truBrain Neuroscientists To Launch More Effective, Healthier Alternative To Energy Drinks On Indiegogo 2
... Madison, Wis. - The WiCell Research Institute , ... controlling all 21 federally funded stem cell lines in ... director. , ,Donley, who led litigation and patent-drafting efforts as ... and strategic direction at a time when the race to ...
... Green has announced a plan to allocate $25 million ... to fund peer-reviewed research into methods of deriving stem cells ... from Gov. Jim Doyle was swift, with the ... faulty research technique. , ,Appearing at the home of Tom ...
... say it's still summer, but for workers the lazy, hazy, ... physically and mentally. So now that we are all rested ... in the workplace? , ,Well, actually neither workers or the ... signs of outright conflict and strife between management and workers, ...
Cached Biology Technology:Donley has growth plans for stem cell bank 2Donley has growth plans for stem cell bank 3Green unveils $25 million stem cell plan 2Green unveils $25 million stem cell plan 3Workplace flexibility: Give and take or tug of war? 2Workplace flexibility: Give and take or tug of war? 3Workplace flexibility: Give and take or tug of war? 4Workplace flexibility: Give and take or tug of war? 5
(Date:7/24/2014)... Environmental cleanup experts gathered at NJIT this week for ... by the U.S. Environmental Protection Agency (EPA) to dredge ... Passaic River. , The EPA has called the plan, ... sediments and capping sections of the riverbed, one of ... material would be dredged and disposed off-site. , "We ...
(Date:7/24/2014)... conducting research into the potato genes that best adapt ... reduction in rainfall and increased extremes of hot and ... genes in order to create new potato varieties that ... also seeking to find out how the current potato ... and higher and lower temperatures. , This research is ...
(Date:7/24/2014)... drug that successfully treated lupus in mice, a biomedical engineer ... to expand his research to a new version of the ... autoimmune diseases., Chandra Mohan, Hugh Roy and Lillie Cranz Cullen ... study in Arthritis Research & Therapy outlining the use of ... reduced the number of cases of lupus-related kidney disease., He ...
Breaking Biology News(10 mins):Experts Weigh the Pros and Cons of a $1.7 Billion EPA Cleanup Plan for the Passaic River at an NJIT 2Neiker-Tecnalia is researching the potato genes that best adapt to climate change 2Biomedical engineer looks at new applications for novel lupus drug 2Biomedical engineer looks at new applications for novel lupus drug 3
... health care issues facing women in eastern Burma (also ... surveys by researchers at the Johns Hopkins Bloomberg School ... 88 percent of women had a home delivery during ... 5 time as likely to receive no antenatal care. ...
... a computer to both store information and process that information. ... hard disk stores information, the central processing unit (CPU) does ... these functions in the same cells neurons leading ... serve these different functions. In a discovery that ...
... Glycomics in collaboration with colleagues at the University ... identifying a broad spectrum treatment for the dreaded ... highly-infectious viruses are the leading cause of severe ... hospitalisations in the developed world, and hundreds of ...
Cached Biology News:Maternal health problems in Myanmar widespread 2SUNY Downstate researchers find that memory storage molecule preserves complex memories 2Researchers find chink in the armor of viral 'tummy bug' 2
Goat polyclonal to CHMP5 ( Abpromise for all tested applications). Antigen: Synthetic peptide: C-NKDGVLVDEFGLPQ, corresponding to C terminal amino acids 202-215 of Human CHMP5 Entrez GeneID: ...
Rabbit polyclonal to Reptin/TIP49B/RUVB2 ( Abpromise for all tested applications). Antigen: Synthetic peptide (Human) Entrez Gene ID: 10856 Swiss Protein ID: Q6FIB9...
Goat polyclonal to ORC2L ( Abpromise for all tested applications). entrezGeneID: 4999 SwissProtID: Q13416...
14-3-3 tau Antibody Shipping Temperature: HOT Storage Temperature: -20C...
Biology Products: