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High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

Craig, R. G. Buckholz, L. R. Madden, P. A. Kellaris, G. R. Davis, B. L. Smiley, J. Cruze, R. Torregrossa, G. Velicelebi and G. P. Thill. 1987. High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. BIO /TECHNOLOGY, Vol 5, 479-485

2. Brierley, R.A., C. Bussineau, R. Kosson, A. Melton and R. S. Siegel. 1992. in Fermentation Development of Recombinant Pichia pastoris Expression the Heterologous Gene: Bovine Lysozyme, Annals New York Academy of Sciences, 350-362

3. Chen, Y., Krol, J., Cino, J., Freedman, D., White, C., and Komives, E., 1996. Continuous Production of Thrombomodulin from a Pichia pastoris Fermentation . Journal Chem. Tech. Biotechnol. Vol. 67, 143-148

4. Clare, J.J., F.B. Rayment, S.P. Ballantine, K. Sreekrishna and M.A.Romanos, 1991. Highlevel expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. BIO/TECHNOLOGY, Vol 9, 455-460

5. Cregg, J.M., T.S. Vedvick and W.C. Raschke. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. BIO/TECHNOLOGY, Vol.11, 905

6. Digan, M. E., S. V. Lair, R. A. Brierley, R. S. Siegel, M. E. Williams, S. B. Ellis, P. A. Kellaris, S. A. Provow, W. S. Craig, G. Velicelebi and M. M. Harpold. 1989. Continuous production of a novel lysozyme via secretion from the yeast, Pichia pastoris. Vol 7, 160-164

7. Tschopp, J. F., G. Sverlow, R. Kosson, W. Craig and L. Grinna. 1987. High-level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris . BI0 /TECHNOLOGY, Vol 5, 1305


Additional Reading
Agrawal, P., G. Koshy and M. Ramseier. 1989. An algorithm for operation a fed-batch fermentor at optimum specific-growth rate. Biot
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