Navigation Links
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

upplementation profile of a P. pastoris culture showing the increased oxygen requirement of the culture.


Table 2:
MEDIUM COMPONENTS AND FEED SOLUTIONS

H3PO4 - 27 ml/L
CaSO4. 2 H2O - 0.9 g/L
K2SO4 - 18 g/L
MgSO4. H2O -15 g/L
KOH - 4.13 g/L
Trace Metals Solution - 4.4 ml/L
Glycerol - 40 g/L

Trace Metals Solution
Cupric sulfate . 5 H2O - 6.0 g/L
Sodium iodide - 0.08 g/L
Manganese sulfate . H2O - 3.0 g/L
Sodium molybdate - 0.2 g/L
Boric acid - 0.02 g/L
Cobalt chloride - 0.5 g/L
Zinc chloride - 20 g/L
Ferrous sulfate . 7 H2O - 65.0 g/L
Biotin - 0.2 g/L
Sulfuric acid - 5.0 ml
Water - to 1.0 liter

Feed Solutions
Glycerol Feed Solution:
50% glycerol with 12 ml/L trace metals solution

Methanol Feed Solution:
100% glycerol with 12 ml/L trace metals solution


Dr. Cino is Product Manager, New Brunswick Scientific, PO Box 4005, Edison, NJ 08818-4005. Phone 800-631-5417. Fax: 732-287-4222. Web: www.nbsc.com. E-mail: cino@nbsc.com. The author acknowledges Yinliang Chen, Jeffrey Krol, Victor Sterkin of New Brunswick Scientific


References
1. Cregg, J.M., J. F. Tschopp, C. Stillman, R. Siegel, M. Akong, W. S.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Yeast Protein Production System Features High Yields and One-Step Purification
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Increase Protein Activity and Yield
6. High Yield Transcription for Every Application
7. Maximize Viral RNA Yield from Biological Fluid
8. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
9. Synthesize High Yields of Biotinylated aRNA
10. Translate High Yields of Active Protein
11. Translate High Yields of Biologically Active Proteins
Post Your Comments:
(Date:11/26/2014)... The North America Thermal Protective Clothing Market ... analysis of the growth trends and revenue forecasts. ... by 2018, growing at a CAGR of 7.1% ... TOC of the North America Thermal Protective Clothing ... in-depth analysis and industry segmentation supported by various ...
(Date:11/26/2014)... On January, 14, 2015, UMBC’s ... D.C. science community to the UMBC campus for a ... the Protein Society, will take place from 9 a.m. ... Through discussion, presentations and workshops, the free symposium ... their role in the industry and the future of ...
(Date:11/26/2014)... Silicon Valley Electronic Data Capture (EDC) ... Alliance , provider of clinical research and data management ... addition to its CRO Partnership Program, Clinovo expands ... India. , “We have found Clinovo’s ClinCapture to ... platform which is both user friendly and easy to ...
(Date:11/26/2014)... SoundConnect , an award winning unified communications ... Darren Suders has joined the team as Director of ... partner program through innovative offerings, consistent partner ... brings more than 10-years of channel telecommunication experience to ... from policy development, to partner education and to expansion. ...
Breaking Biology Technology:The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 2The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 3RPM Alliance Joins Clinovo’s CRO Partnership Program, Further Strengthening Its Presence in Southern California and India 2SoundConnect Appoints Director of Channel Development & Sales 2
... Eric Schmidt, then the CTO of Sun Microsystems, gave ... language called Java. A thoroughly modern development platform for ... Java promised to ease programming headaches, the biggest of ... another. , ,Today, we can debate whether Java ...
... believes in reincarnation - in the business sense. , ,What ... reiterations of his company, Paragon Development Systems, Inc. , ... with a very important streak intact - 19 consecutive years ... a memory chip broker that managed to survive 20 years ...
... Madison, Wis. - Gov. Jim Doyle ... million in state funds for the development of renewable ... - which includes tax credits, low-interest loans, and grants ... the sale of state bonds - would leverage an ...
Cached Biology Technology:Java's undeniable impact on the Web 2Java's undeniable impact on the Web 3PDS survives with technological reinvention 2PDS survives with technological reinvention 3PDS survives with technological reinvention 4PDS survives with technological reinvention 5Doyle announces $80M renewable energy strategy 2Doyle announces $80M renewable energy strategy 3Doyle announces $80M renewable energy strategy 4
(Date:11/7/2014)... associate professor, biomedical engineering, in the Grove School ... York, have identified a molecule that could lead ... aggressive forms of breast cancer. , Triple negative ... owing to aggressive proliferation and metastasis and a ... team, discovered the overexpression of intercellular adhesion molecule-1 ...
(Date:11/6/2014)... for a predator. Except when that predator runs so fast ... to its size, is the fastest creature on Earth. Some ... second (at about five miles per hour). The fastest human ... gold from the tiger beetle, a person would have to ... has a problem. At peak speeds, everything becomes a blur. ...
(Date:11/4/2014)... University of Utah engineers developed the first room-temperature fuel ... electricity without needing to ignite the fuel. These new ... off-grid power and sensors. , A study of the ... Society journal ACS Catalysis . , Fuel cells ... a fuel and an oxygen-rich source such as air. ...
Breaking Biology News(10 mins):The tiger beetle: Too fast to see 2Jet-fueled electricity at room temperature 2
... understand molecular mechanisms underlying various diseases, notably cancer, have ... of patients for the expression status of thousands of ... of each gene in the genome, measured as the ... Since each of the tens of thousands of genes ...
... essayist as we have."New York Times Book Review "Carl ... today. A Planet of Viruses is an important primer on ... other times shocking, and always accessible. Whether discussing the common ... oceans, or the world,s viral future as seen through our ...
... 5, 2011 In their eagerness to cut nitrogen ... of Mexico, people have often sought simple explanations for ... or farmers who apply too much fertilizer, which then ... research that examined phosphorus loading from all 1768 counties ...
Cached Biology News:Medusa-structure of gene regulatory network: Dominance of transcription factors in cancer subtypes 2Medusa-structure of gene regulatory network: Dominance of transcription factors in cancer subtypes 3Medusa-structure of gene regulatory network: Dominance of transcription factors in cancer subtypes 4A planet of viruses 2Study probes sources of Mississippi River phosphorus 2Study probes sources of Mississippi River phosphorus 3
Contained in EZ-Tn5™ Kits and are available separately for sequencing of an insertion site....
Request Info...
Rabbit polyclonal to Syntrophin alpha 1 ( Abpromise for all tested applications). entrezGeneID: 6640 SwissProtID: Q13424...
Mouse monoclonal [4i97] to HLA Class 1 A25 ( Abpromise for all tested applications). Antigen: Full length protein (Human): HLA Class 1 Antigen-A25. Swiss Protein ID: P18462...
Biology Products: