Navigation Links
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

upplementation profile of a P. pastoris culture showing the increased oxygen requirement of the culture.


Table 2:
MEDIUM COMPONENTS AND FEED SOLUTIONS

H3PO4 - 27 ml/L
CaSO4. 2 H2O - 0.9 g/L
K2SO4 - 18 g/L
MgSO4. H2O -15 g/L
KOH - 4.13 g/L
Trace Metals Solution - 4.4 ml/L
Glycerol - 40 g/L

Trace Metals Solution
Cupric sulfate . 5 H2O - 6.0 g/L
Sodium iodide - 0.08 g/L
Manganese sulfate . H2O - 3.0 g/L
Sodium molybdate - 0.2 g/L
Boric acid - 0.02 g/L
Cobalt chloride - 0.5 g/L
Zinc chloride - 20 g/L
Ferrous sulfate . 7 H2O - 65.0 g/L
Biotin - 0.2 g/L
Sulfuric acid - 5.0 ml
Water - to 1.0 liter

Feed Solutions
Glycerol Feed Solution:
50% glycerol with 12 ml/L trace metals solution

Methanol Feed Solution:
100% glycerol with 12 ml/L trace metals solution


Dr. Cino is Product Manager, New Brunswick Scientific, PO Box 4005, Edison, NJ 08818-4005. Phone 800-631-5417. Fax: 732-287-4222. Web: www.nbsc.com. E-mail: cino@nbsc.com. The author acknowledges Yinliang Chen, Jeffrey Krol, Victor Sterkin of New Brunswick Scientific


References
1. Cregg, J.M., J. F. Tschopp, C. Stillman, R. Siegel, M. Akong, W. S.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Yeast Protein Production System Features High Yields and One-Step Purification
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Increase Protein Activity and Yield
6. High Yield Transcription for Every Application
7. Maximize Viral RNA Yield from Biological Fluid
8. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
9. Synthesize High Yields of Biotinylated aRNA
10. Translate High Yields of Active Protein
11. Translate High Yields of Biologically Active Proteins
Post Your Comments:
(Date:7/30/2014)... , July 30, 2014 Regulus Therapeutics ... company leading the discovery and development of innovative medicines ... results and highlights for the quarter ended June 30, ... financial markets close. Regulus will host ... 2014, at 5:00 pm Eastern Daylight Time to discuss ...
(Date:7/30/2014)... N.Y. and PARIS , ... and Sanofi (EURONEXT: SAN and NYSE: SNY) today announced ... and Drug Administration (FDA) rare pediatric disease priority review ... submission for alirocumab.  The priority review voucher entitles the ... provides for an expedited 6-month review from the filing ...
(Date:7/30/2014)... 2014 W. R. Grace & ... the REVELERIS® Prep purification system , a ... and other researchers to perform both flash and ... , With the REVELERIS® Prep purification system, researchers ... modes with a simple touch of the screen, ...
(Date:7/30/2014)... , July 30, 2014  Decision Resources ... and medical directors in the United ... of biosimilar market penetration will be the level ... (MCOs) and the expected lower cost to patients. ... biosimilars that meet their pricing expectations in order ...
Breaking Biology Technology:Regulus Announces Timing for Second Quarter 2014 Financial Results Webcast and Conference Call 2Regulus Announces Timing for Second Quarter 2014 Financial Results Webcast and Conference Call 3Regeneron and Sanofi Announce Plan to Use Priority Review Voucher For Alirocumab U.S. FDA Submission 2Regeneron and Sanofi Announce Plan to Use Priority Review Voucher For Alirocumab U.S. FDA Submission 3Regeneron and Sanofi Announce Plan to Use Priority Review Voucher For Alirocumab U.S. FDA Submission 4Regeneron and Sanofi Announce Plan to Use Priority Review Voucher For Alirocumab U.S. FDA Submission 5Regeneron and Sanofi Announce Plan to Use Priority Review Voucher For Alirocumab U.S. FDA Submission 6Grace Launches New REVELERIS® Prep Purification System 2Surveyed U.S. Payers Expect Cost Savings to MCOs and Patients to be the Strongest Drivers of Biosimilar Uptake 2Surveyed U.S. Payers Expect Cost Savings to MCOs and Patients to be the Strongest Drivers of Biosimilar Uptake 3
... March 17 The following was issued,today by the ... president & CEO, Greater Baltimore Committee, 140 company ... WHAT:, The third annual Greater Baltimore Region Bioscience ... 8:00 a.m. / Breakfast & Registration, 8:45 - ...
... 17 Mercury Therapeutics,Inc. (MTI), a company ... targeted drugs, announces that it has retained ... on its strategic and partnering,opportunities, with the ... This appointment follows a significant amount ...
... ST. LOUIS, March 17 (Nasdaq: SIAL ) ... holding its 2008 Annual,Business Review on Wednesday, March 19, ... at 2909 Laclede, St. Louis, Mo. 63103, The ... by 12:30 p.m. CDT,and will also be webcast over ...
Cached Biology Technology:Greater Baltimore Committee to Announce Winners of Third Baltimore Region Bioscience Awards at Ceremonies on March 18 2Mercury Therapeutics, Inc: Marquant Appointed to Advise on Strategic and Partnering Opportunities 2Mercury Therapeutics, Inc: Marquant Appointed to Advise on Strategic and Partnering Opportunities 3
(Date:7/30/2014)... Queen in Lewis Carroll,s Through the Looking Glass, ... University of Singapore improved a 35-year-old ecology model ... to millions of years. , The new model, ... the "Red Queen Effect," an evolutionary hypothesis introduced ... suggests that organisms must constantly increase their fitness ...
(Date:7/30/2014)... A deep-sea octopus protected and tended her eggs until ... published July 30, 2014 in the open-access journal ... Aquarium Research Institute and colleagues. , Octopuses typically have ... females protect and take care of their fertilized eggs ... eggs for 1 to 3 months, but little is ...
(Date:7/30/2014)... Chemical Society (ACS) announced today that David L. Kaplan, ... Biomaterials Science & Engineering as editor-in-chief. With the ... new journal will feature high-quality research in the rapidly ... naturally derived materials that interact with living or biological ... reflects the tremendous growth in the field of biomaterials ...
Breaking Biology News(10 mins):Classic Lewis Carroll character inspires new ecological model 2Classic Lewis Carroll character inspires new ecological model 3Deep-sea octopus has longest-known egg-brooding period 2ACS Biomaterials Science & Engineering: Brand-new journal launches in 2015, names editor 2
... is also available in Chinese on EurekAlert! ... A new laser-beam steering system that aims and focuses bursts ... has been demonstrated by collaborating researchers from Duke University and ... Applied Physics Letters , published by the American Institute of ...
... the RMS Titanic, which is contributing to its deterioration. ... the exterior of ships and underwater metal structures such ... findings in the latest issue of the International ... 8 December, isolated the micro-organisms from a ,rusticle, collected ...
... book, Illinois Birds: A Century of Change, literally took 100 ... Illinois was conducted from 1906-1909. It was repeated from 1956-1958 ... When the 100-year anniversary of that first survey was approaching, ... seized the window of opportunity to do it again and ...
Cached Biology News:Tiny laser light show illuminates quantum computing 2New microscopic life aboard the RMS Titanic 2New book on 100 years of Illinois birds 2New book on 100 years of Illinois birds 3New book on 100 years of Illinois birds 4
...
... Rabbit Antibody to CRF ... with the ovine CRF sequence ... Cells and fibres are found ... of rat brain in colchicine ...
...
Alexa Fluor 647 anti-mouse Qa-2...
Biology Products: