Navigation Links
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

th limiting nutrients can be pumped into the vessel at a rate that is capable of replenishing the nutrients that are depleted. Shakers can only run in a batch mode, meaning that the growth of the cells is limited by the nutrients present in the medium at the time of inoculation. The fermentors fed-batch mode further enables methanol flow rates to be controlled to condition the cells to the presence of the methanol, as well as provide methanol at the proper rate to allow addition of just enough methanol for protein synthesis while preventing excess methanol addition which can cause toxicity.

Researchers have found that optimum protein production in P. pastoris occurs at 30C, and that all protein expression ceases at 32C. However, high heat loads occur when P. pastoris is actively growing or expressing high levels of protein. In actively growing shake flask cultures, it is not uncommon for the temperature to increase 25C if left uncontrolled. Therefore, it is imperative that all aspects of the fermentor, including temperature controller, heat exchanger, vessel and piping, must be designed to regulate temperature for optimum protein production. In addition to the fermentors internal controller, an external bioprocessing software (BioCommand, New Brunswick Scientific) is routinely used to supervise the process, as well as to provide optimal nutrient feed rates, based on either the current status of the culture or to actuate pre-determined control scenarios.


Fermentation Protocol
Research was conducted in BioFlo 3000 benchtop fermentors (New Brunswick Scientific) (Figure 1) with interchangeable, autoclavable vessels of 1.25 to 10 L working volume, as well as in a BioFlo 4500 fermentors with sterilizable-in-place vessels of 15 L and 20 L working volume, (New Brunswick Scientific
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Yeast Protein Production System Features High Yields and One-Step Purification
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Increase Protein Activity and Yield
6. High Yield Transcription for Every Application
7. Maximize Viral RNA Yield from Biological Fluid
8. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
9. Synthesize High Yields of Biotinylated aRNA
10. Translate High Yields of Active Protein
11. Translate High Yields of Biologically Active Proteins
Post Your Comments:
(Date:1/15/2014)... Jan. 15, 2014  Bill Jacobs Automotive, a group of car ... up with the Heartland Blood Center and offering free oil ... drive takes place Saturday, Jan. 18 at the Bill Jacobs ... (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The ...
(Date:1/15/2014)... 2014 Look inside the new Preferred ... the lab, from fluid handling to instruments to supplies. ... when you order. , Preferred Solutions features a ... the L/S® model for precise flow control and dispensing ...
(Date:1/15/2014)... RedBrick Health , a fast-growing leader in consumer health ... EmblemHealth , the New York -based ... RedBrick Compass health assessment, combined with the RedBrick Journeys ... EmblemHealth is among the first health plans in the ...
(Date:1/15/2014)... January 15, 2014 More than 5 ... about 1 in 3 seniors will die with Alzheimer’s ... These jaw-dropping figures have shocked many Americans into looking ... help prevent these tragic age-related cognitive disorders. Jonathan Weisman, ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2
... it not been for the war in Iraq, 2003 might ... forgot. Certainly, in the world of information technology, that may ... happened slowly. Customers took their time. But for some activity ... then, must explain why the predictions I so carefully made ...
... robotic rover Spirit, which bounced to a near pinpoint ... 3) after a seven-month flight, promises a trove of ... , ,John Valley, a UW-Madison professor of geology and ... to be from Mars, expects the latest mission to ...
... - Fiserv Health, an operating group of Fiserv, ... a care management company targeting the self-funded health care ... health plan management, claims processing and administration services for ... addition of Avidyn Health, Fiserv Health now has an ...
Cached Biology Technology:The Year Ahead in IT 2The Year Ahead in IT 3The Year Ahead in IT 4The Year Ahead in IT 5Fiserv Launches Avidyn Health to Target Healthcare Costs 2
(Date:4/17/2014)... In the most densely forested and most densely populated ... of human needs, values and practices. Disturbances associated with ... agriculture and development, have set the stage for management ... study reports. , The report Five anthropogenic factors ... in the Northern United States was published ...
(Date:4/17/2014)... of stem cell therapies to cure a variety of ... populations based on cell surface markers. Researchers from the ... is highly expressed in a type of stem cells ... in an article in BioResearch Open Access , ... The article is available free on the ...
(Date:4/17/2014)... detection cartridge developed at Sandia National Laboratories and recently ... faster and cheaper. , Bacillus anthracis , ... soils all over the world and can cause serious, ... The bacteria can survive in harsh conditions for decades. ... occur through skin contact, inhalation of spores or eating ...
Breaking Biology News(10 mins):Five anthropogenic factors that will radically alter northern forests in 50 years 2Five anthropogenic factors that will radically alter northern forests in 50 years 3Pocket-sized anthrax detector aids global agriculture 2Pocket-sized anthrax detector aids global agriculture 3
... men prefer beer when they drink alcohol, taking in at ... of the beverage. Far fewer (around five percent) prefer wine. ... beverages and a riskier approach to alcohol or other substances? ... Preventive Medicine at the University of Zurich and Lausanne University ...
... has shown for the first time evidence of ... bipolar disorder, major depressive disorder and schizophrenia and ... attention deficit-hyperactivity disorder (ADHD), according to a study ... Advance Online publication. The root cause of ...
... NEW YORK CITY Morning sickness, shiny hair, and ... what expectations do pregnant women impose on their ... perspectives on pregnancy? Danielle Bessett, an assistant ... present her research on this issue at the 108th ...
Cached Biology News:Young beer-drinkers binge-drink more frequently 2New patterns found in the genetic relationship of 5 major psychiatric disorders 2Cultural mythologies strongly influence women's expectations about being pregnant 2Cultural mythologies strongly influence women's expectations about being pregnant 3
... The Freedom EVO series offers versatile liquid ... successfully automate a wide variety of life ... four different base sizes (75, 100, 150 ... access to a platform that will advance ...
... and conveniently controls the temperature at your lab ... levels up to 99 C*, with the "plus" ... as 5 C. The thermoblocks for 0.2 ml**, ... microplates enable a high degree of flexibility for ...
384 Well PCR Plate Carrier for Thermo-Sealer...
... Cell Cultures (ECACC) has been offering training ... government institutes for over seven years. We ... introduce candidates to cell culture and a ... specialist areas such as planning and management ...
Biology Products: