Navigation Links
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

otic expression systems, Pichia offers many advantages, because it does not have the endotoxin problem associated with bacteria nor the viral contamination problem of proteins produced in animal cell culture. Furthermore, P. pastoris can utilize methanol as a carbon source in the absence of glucose. The P. pastoris expression system uses the methanol-induced alcohol oxidase (AOX1) promoter, which controls the gene that codes for the expression of alcohol oxidase, the enzyme which catalyzes the first step in the metabolism of methanol. This promoter has been characterized and incorporated into a series of P. pastoris expression vectors. Since the proteins produced in P. pastoris are typically folded correctly and secreted into the medium, the fermentation of genetically engineered P. pastoris provides an excellent alternative to E. coli expression systems. A number of proteins have been produced using this system, including tetanus toxin fragment, Bordatella pertussis pertactin, human serum albumin and lysozyme. (3 - 7).


Minimizing Growth Limiting Factors
Another advantage of Pichia pastoris is a prolific growth rate. Therefore, it would seem easy enough to culture it in a shake flask. This seeming advantage, however, can pose a host of problems, including pH control, oxygen limitation, nutrient limitation and temperature fluctuation. Researchers at New Brunswick Scientific (Edison, NJ) found that by switching from a shaker to a fermentor, protein production in Pichia could be increased by over 140% (3). The fermentor enables dissolved oxygen (DO) levels to be raised, not just by increasing agitation, but by increasing air flow, by supplementing the air stream with pure oxygen, or by doing all three either in series or in parallel. Nutrient limitation can also be minimized, since fermentors can be run in fed-batch mode, where fresh media or grow
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Yeast Protein Production System Features High Yields and One-Step Purification
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Increase Protein Activity and Yield
6. High Yield Transcription for Every Application
7. Maximize Viral RNA Yield from Biological Fluid
8. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
9. Synthesize High Yields of Biotinylated aRNA
10. Translate High Yields of Active Protein
11. Translate High Yields of Biologically Active Proteins
Post Your Comments:
(Date:7/6/2015)... July 6, 2015  Emdeon Inc. today ... definitive agreement to acquire Altegra Health, a ... that combine data aggregation and analytics with ... achieve actionable insights and improved management for ... Health,s risk adjustment and quality analytics and ...
(Date:7/6/2015)... June 29, 2015 ... the addition of the "Biotechnology for the ... Biotechnology for the Non-Biotechnologist, ideal for non-Scientists and ... techniques and potential of biotechnology. Ideal ... basic theory, principles, techniques, and potential of biotechnology ...
(Date:7/6/2015)... N.J. , July 6, 2015 Navitas, a leading Regulatory ... Latin America hub in Bogota, Colombia . ... of the many benefits provided by Colombia , the ... Logo - http://photos.prnewswire.com/prnh/20150703/228239LOGO ... location, allows US based clients to communicate with Navitas regulatory professionals ...
(Date:7/5/2015)... Brooklyn, NY (PRWEB) , ... July 06, 2015 , ... ... spectrophotometers. If a user had a high-end machine such as a Varian Cary 4000-7000, ... charm. So widespread was this design that even NIST used them for their SRM-2031 ...
Breaking Biology Technology:Emdeon to Acquire Altegra Health 2Emdeon to Acquire Altegra Health 3Emdeon to Acquire Altegra Health 4Navitas Launches Latin American Regulatory Operations Hub in Bogota, Colombia 2Navitas Launches Latin American Regulatory Operations Hub in Bogota, Colombia 3FireflySci Simplifies the Calibration of UV Spectrophotometers with FUV Photometric Accuracy Standards 2FireflySci Simplifies the Calibration of UV Spectrophotometers with FUV Photometric Accuracy Standards 3
... Inc. announced that the launch of a new ... of cancer treatments.  BioSpeciFx® is composed of carefully ... biomarker tests that identify critical molecular targets within ... by BioSpeciFx® in combination with Precision,s sophisticated drug ...
... Texas, April 7, 2011 Wound Management Technologies, Inc., ... leader in advanced wound care solutions, today announced that ... care products within key international markets, has received new ... gel products.  In Lebanon, the subsidiary just completed a ...
... Reportlinker.com announces that a new market research report ... Industrial Biotechnology China News 1103 ... government was reported to continue intensifying the demand ... (2011-2015) Plan. This act is expected to help ...
Cached Biology Technology:Precision Therapeutics Announces New Tumor Profiling Product to Augment Utility of ChemoFx® and Further Personalize Cancer Treatment 2WNDM Secures Additional International Sales; $150 K in New Orders for 2011 2WNDM Secures Additional International Sales; $150 K in New Orders for 2011 3Reportlinker Adds Industrial Biotechnology China News 1103 2Reportlinker Adds Industrial Biotechnology China News 1103 3
(Date:6/24/2015)... 2015 The biologics safety testing market is ... the market include growth of the pharmaceutical and biological ... Over the years, the number of drug approvals in ... the FDA approved 225 drug applications and Health Canada ... Europe accounted for 3,822 of the pharmaceutical ...
(Date:6/24/2015)... , June 24, 2015 Biometry authentication ... emerging biometric password solution one face in. ... one face in, entering the expanding ... and affordable multifactor-authentication biometric answer to the password ... provided in partnership with KeyLemon, one face in ...
(Date:6/23/2015)... PALM BEACH GARDENS, Fla. , June ... of multi-factor identity management and authentication solutions, today ... Altus strong, multi-factor authentication solution.  The ... ease of use of the DigitalPersona Altus ... and biometric reader compatibility. In ...
Breaking Biology News(10 mins):Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 2Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 3Biological Safety Testing Market by Application, Product, & Test - Global Forecast to 2019 4KeyLemon Announces Partnership With Biometric Password Solution onefacein 2Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2
... out of the University of Alberta for those who may have ... medical researchers have found a way to reduce the amount of ... from food the body metabolizes, a key discovery that could lead ... 2 diabetes and heart disease related to obesity. In ...
... A Web site is a better source of information ... to a study of low-income mothers reported in the January ... http://www.jneb.org/) . Led by Kami J. Silk, Ph.D., of ... mothers with nutrition information in different formats. One group ...
... cause of sudden cardiac death even when it isnt overdosed ... chronic pain or drug addiction withdrawal, a new study by ... findings, described in the January 2008 issue of The American ... sudden cardiac deaths in the greater Portland, Ore., metropolitan area ...
Cached Biology News:University of Alberta researchers report breakthrough in lowering bad cholesterol, fatty acid levels 2For nutrition info, moms like the Web best 2Methadone even at therapeutic levels can kill 2Methadone even at therapeutic levels can kill 3