Navigation Links
High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

erforming closely to Agencourt's internal procedures. Figure 1 is a sequence trace from a sample purified on the Sciclone. Read lengths are comparable at 789 versus 779 bp for the DNATrack and the Sciclone, respectively. Each system gave a 94% pass rate out of 384 samplings (4 x 96 well plates). Nucleic acid yield for the two systems are roughly 16 ng/μL by PicoGreen binding analysis. Results from electrophoretic separation of the purified nucleic acid are presented in Figure 2.

Discussion

The Agencourt CosMCPrep Kit, Agencourt ring magnet plate, and Sciclone ALH 3000 can easily purify plasmids. The system does not require external hardware such as a centrifuge or vacuum pump/filter. The ring magnet locator easily allows complete removal of supernatant. Selective removal of lysate was accomplished by blowing air into the wells of the culture plate at the 12 o' clock position. Bubbles push residual cell material to the top of the well while leaving clean lysate at the bottom for easy transfer.

PicoGreen and sequencing data demonstrates that the CaliperLS Sciclone ALH performs comparably to Agencourt's procedures and hardware. Sequencing lengths and total yield were comparable on the two systems.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. RNAi for High Throughput Analysis
Post Your Comments:
(Date:9/21/2014)... first time, scientists have discovered how to produce ultra-thin ... stiffness greater than that of today,s strongest nanotubes and ... team led by John V. Badding, a professor of ... the 21 September 2014 issue of the journal ... view, our discovery is intriguing because the threads we ...
(Date:9/19/2014)... (Nasdaq: ITMN ) today announced that it ... litigation in the Delaware Court of Chancery relating to ... August 22, 2014, among Roche Holdings, Inc., Klee Acquisition ... Memorandum of Understanding, the Company agreed to make certain ... the amendment to the Company,s Schedule 14D-9 to be ...
(Date:9/19/2014)... NEW YORK , Sept. 19, 2014  An ... common forms of inherited neuropathies – Charcot-Marie-Tooth disease (CMT) ... feet and legs, causing extreme muscle atrophy, weakness, and ... in the US and millions worldwide. CMT is a ... use of their legs, requiring mobility devices such as ...
(Date:9/19/2014)... Barnhardt Manufacturing Company has earned ... Organic Cotton . The USDA Certified Biobased Product Label ... meets or exceeds levels set by USDA. Biobased products ... in significant part of agricultural, forestry, or marine ingredients. ... to be immediately identified as a USDA certified BioPreferred ...
Breaking Biology Technology:Smallest possible diamonds form ultra-thin nanothreads 2Smallest possible diamonds form ultra-thin nanothreads 3Smallest possible diamonds form ultra-thin nanothreads 4InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 2InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 3InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 4InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 5InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 6InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 7InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 8InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 9InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 10InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 11InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 12InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 13InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 14InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 15InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 16InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 17InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 18InterMune Reports Memorandum of Understanding in Connection with Consolidated Delaware Action 19September is Charcot-Marie-Tooth (CMT) Awareness Month - Scheduled events, activities and important partnerships announced throughout the month! 2Barnhardt Manufacturing Company Earns USDA Certified Biobased Product Certification and Label for Organic Cotton 2
... , a biopharmaceutical company developing cancer therapies, has announced ... been selected for preclinical testing and human clinical trials. ... safety assessment, it would submit to the U.S. ... application in late 2007, with human clinical trials expected ...
... Milwaukee, Wis - Understanding our genetic makeup ... to the point where DNA testing services, including ... shaping their own health management. Patients, however, will ... knowledge because most healthcare facilities are not yet ...
... The drive to make healthcare information technology interoperable ... applications, and a medical information technology company headquartered ... TeraMedica is lending its expertise to developing ... for the healthcare industry. Specifically, the expertise of ...
Cached Biology Technology:Healthcare slow to adopt genetic advances 2Healthcare slow to adopt genetic advances 3TeraMedica embraces open source project 2
(Date:9/19/2014)... will study multifunctional and specialized spinal cord nerve ... Science Foundation grant in the amount of $680,000 ... in the Department of Biology, OU College of ... between the multifunctional and specialized spinal cord nerve ... what connections they make. Berkowitz will collaborate ...
(Date:9/19/2014)... years has adapted to the arctic cold, and then you ... for you. , This is happening to the small white ... called nonylphenol, comes from the use of certain detergents, pesticides ... disruptor, but when entering the worm it has another dangerous ... in its body from cold damage. , Enchytraeus albidus ...
(Date:9/19/2014)... new study shows that legume plants regulate their ... molecules that are transmitted through the plant structure ... number of bacteria-holding nodules in the roots. This ... National Institute for Basic Biology, the Graduate University ... for Sustainable Resource Science in Japan. , ...
Breaking Biology News(10 mins):Environmental pollutants make worms susceptible to cold 2Environmental pollutants make worms susceptible to cold 3For legume plants, a new route from shoot to root 2
... has found that a combination of methods that help ... their physical health and lessen symptoms of depression. Published ... , the study indicates that clinicians should consider using ... poor nutrition and signs of depression. In patients ...
... areas from fishing increases density and biomass of fish ... systematic review published in BioMed Central,s open access journal ... area was also dependent on its size and on ... significant ecological benefits. Marine ecosystems worldwide are suffering ...
... new study, biologists will investigate the connection between amphibians, ... record number of frogs, toads and salamanders worldwide., This ... grant from the National Science Foundation (NSF) to explore ... called Batrachochytrium dendrobatidis (Bd). This harmful fungus ...
Cached Biology News:Helping dementia patients remember to eat well improves physical and mental health 2How much protection is enough? 2Biologists explore link between amphibian behavior and deadly disease 2Biologists explore link between amphibian behavior and deadly disease 3
... Microsphere/Microarray Stabilizer is an aqueous solution that ... non-toxic chemicals in a PBS buffer (phosphate ... This product contains a combination of 0.02% ... StabilCoat Plus Stabilizer provides optimum performance ...
... The mini incubation trays are ... to screen antigens that have ... Each tray has eight 10.5 ... that accommodate strips cut from ...
... Protein Purification Plates are designed for quick ... The 96-well plates feature two 0.25mm holes ... each well. The wells remain liquid-tight during ... during which time the protein diffuses from ...
... 20 Plasma Immunodepletion Kit specifically removes 20 ... plasma or serum in preparation for further ... into the plasma proteome whether you use ... chromatography or go straight to mass spectrometry. ...
Biology Products: