Navigation Links
High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

erforming closely to Agencourt's internal procedures. Figure 1 is a sequence trace from a sample purified on the Sciclone. Read lengths are comparable at 789 versus 779 bp for the DNATrack and the Sciclone, respectively. Each system gave a 94% pass rate out of 384 samplings (4 x 96 well plates). Nucleic acid yield for the two systems are roughly 16 ng/μL by PicoGreen binding analysis. Results from electrophoretic separation of the purified nucleic acid are presented in Figure 2.

Discussion

The Agencourt CosMCPrep Kit, Agencourt ring magnet plate, and Sciclone ALH 3000 can easily purify plasmids. The system does not require external hardware such as a centrifuge or vacuum pump/filter. The ring magnet locator easily allows complete removal of supernatant. Selective removal of lysate was accomplished by blowing air into the wells of the culture plate at the 12 o' clock position. Bubbles push residual cell material to the top of the well while leaving clean lysate at the bottom for easy transfer.

PicoGreen and sequencing data demonstrates that the CaliperLS Sciclone ALH performs comparably to Agencourt's procedures and hardware. Sequencing lengths and total yield were comparable on the two systems.


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. RNAi for High Throughput Analysis
Post Your Comments:
(Date:10/18/2014)... (PRWEB) October 18, 2014 The Asian Electronic ... market in Asian with analysis and forecast of revenue. The ... to grow to $2,328.0 million by 2018, at a CAGR ... TOC of the Asian Electronic Medical Record (EMR) market, to ... provides a glimpse of the segmentation of the market in ...
(Date:10/18/2014)... The report " Cloud Analytics Market [Cloud ... Machine; Video; Predictive Analytics: Global Advancements, Delivery Models, ... (2013 - 2018) ", defines and segments the ... with an in-depth analysis and forecasting of market ... this market, various restraints, and opportunities impacting it ...
(Date:10/17/2014)... 16—As in Alice,s journey through the looking-glass to Wonderland, ... surprising and unexpected ways, including a new class of ... reported today in The Optical Society,s (OSA) new high-impact ... for the first time, a new type of mirror ... reflects infrared light by using an unusual magnetic property ...
(Date:10/17/2014)... 2014 Bioquell, provider of germ-eliminating ... health facilities around the world to decontaminate locations ... Among the many places the hydrogen peroxide vapor ... Hospital Dallas and at the National Institutes of ... have been utilized to decontaminate patient rooms, equipment, ...
Breaking Biology Technology:The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 2The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 3The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 4The Asian Electronic Medical Record (EMR) market is estimated to grow to $2,328.0 million by 2018 - New Report by MicroMarket Monitor 5Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 2Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 3Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 4Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 5Magnetic mirrors enable new technologies by reflecting light in uncanny ways 2Magnetic mirrors enable new technologies by reflecting light in uncanny ways 3Magnetic mirrors enable new technologies by reflecting light in uncanny ways 4Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 2Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 3Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 4Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 5Bioquell’s Vapor Producing Robots Selected to Combat Ebola in Hospitals Around the World 6
... , New Vitality hrGFP expression vectors , ... Expression Vectors Employ Stable, High-Level Fluorescence , Humanized Renilla GFP Reporter , ... , ... , Stratagene , , ...
... of FLAG -Tagged Fusion Proteins ,,,,, Detection of expressed ... of FLAG -Tagged,Fusion Proteins , Qiyi ... Weiping Yang ,Stratagene , Stratagenes TagDetect FLAG ... an easy-to-use assay kit for detecting FLAG epitope-tagged fusion,proteins. To ...
... assists in detecting SNPs,quickly and easily , ... University of Colorado Health Sciences Center, ... custom molecular beacons ff, ,to detect a single ... clear discrimination among the different genotypes carried in,family members. This ...
Cached Biology Technology:New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence , Humanized Renilla GFP Reporter 2New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence , Humanized Renilla GFP Reporter 3New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence , Humanized Renilla GFP Reporter 4New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence , Humanized Renilla GFP Reporter 5New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence , Humanized Renilla GFP Reporter 6New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence , Humanized Renilla GFP Reporter 7Simple, Sensitive, and Rapid Detection of FLAG -Tagged,Fusion Proteins 2Simple, Sensitive, and Rapid Detection of FLAG -Tagged,Fusion Proteins 3Simple, Sensitive, and Rapid Detection of FLAG -Tagged,Fusion Proteins 4Genotyping Single Nucleotide Polymorphisms with Molecular Beacons 2Genotyping Single Nucleotide Polymorphisms with Molecular Beacons 3Genotyping Single Nucleotide Polymorphisms with Molecular Beacons 4Genotyping Single Nucleotide Polymorphisms with Molecular Beacons 5Genotyping Single Nucleotide Polymorphisms with Molecular Beacons 6
(Date:10/15/2014)... to an unexpected extent. The outbreak does not follow ... shows a new disease dynamic in regions, where it ... German National Academy of Sciences Leopoldina, acatech – the ... of the German Academies of Sciences and Humanities have ... In the statement the academies call for the following ...
(Date:10/15/2014)... , Oct. 15, 2014   Neurotechnology , ... announced the availability of the VeriLook Surveillance ... 3.0 provides real-time biometric face identification using live ... surveillance cameras. The new version not only identifies ... people from objects while they are moving through ...
(Date:10/14/2014)... fly genome for the first time, revealing robust immune ... thrives in pathogen-rich dung piles and garbage heaps. , ... Genome Biology , will increase understanding of house fly ... to resist insecticides, which could lead to novel control ... transmit more than 100 human and animal diseases, including ...
Breaking Biology News(10 mins):Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3House fly genome reveals expanded immune system 2
... September 9, 2013 Cytos Biotechnology Ltd (SIX:CYTN) ... with CYT003, a first-in-class immune modulator in clinical development as ... of data published in the March issue of The ... today at the European Respiratory Society Annual Congress in ...
... East Anglia shows that rising ocean temperatures will upset natural ... an important role in the ocean,s carbon cycle by removing ... storing it deep under the sea isolated from the ... Nature Climate Change reveal that water temperature has ...
... more accurate and efficient DNA sequencing is the main focus ... grants nearly $17 million to eight research teams ... Institute (NHGRI),s Advanced DNA Sequencing Technology program, which was launched ... technology shows great promise, but it is still a new ...
Cached Biology News:Cytos Biotechnology Presents Additional Results From Phase 2a Study of CYT003 for the Treatment of Allergic Asthma 2Cytos Biotechnology Presents Additional Results From Phase 2a Study of CYT003 for the Treatment of Allergic Asthma 3Cytos Biotechnology Presents Additional Results From Phase 2a Study of CYT003 for the Treatment of Allergic Asthma 4Cytos Biotechnology Presents Additional Results From Phase 2a Study of CYT003 for the Treatment of Allergic Asthma 5Climate change will upset vital ocean chemical cycles 2New NIH awards focus on nanopore technology for DNA sequencing 2New NIH awards focus on nanopore technology for DNA sequencing 3New NIH awards focus on nanopore technology for DNA sequencing 4
... LC Sciences provides a unique new tool, ... synthesis serving diverse needs in genomics, proteomics, ... OligoMix is a MIXTURE of a large ... delivered in a single microtube. OligoMix is ...
... 2D is an automated, two-dimensional fractionation system ... Part of Beckman Coulters ProteomeLab family ... in just hours. The system effectively ... cell lysates, addressing a key challenge in ...
... The Excalibur™ mid-, near- and far-infrared ... truly research-grade performance in their class. Yet ... price. Features: Highest-energy throughput: Having ... the source compared to other conventional FT-IRs ...
... Rapid-Screen cDNA Library Panels were designed for ... sets of PCRs to identify the desired ... performed in a 96-well "Master Plate," where ... clones. Having identified the positive well(s) by ...
Biology Products: