Navigation Links
High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

arting Materials:

- DH10B Cells
- 1 Rack of 100 μL tips per sample plate
- 1 Rack of 200 μL tips per sample plate

Sciclone Procedure

Methodology

1. Attach 100 μL tips
2 . Add 95 μL Resuspension Solution and shake @ 1200-1500 rpm for 4 minutes
3. Add 100 μL Lysis Solution and shake @ 800-1000 rpm for 5 minutes
4. Add 100 μL Neutralization Solution and shake @ 800-1000 rpm for 10 minutes
5. Slowly dispense 150 μL of air to gently bubble material away from the tips. This is accomplished by lowering the tips to a height just below the top of the well, then moving back to the 12 o'clock position and lowering the tips to the bottom of the well and dispensing the air
6. After a 3 second pause, aspirate 110 μL of Lysate and transfer to a 96-well round bottom microplate
7. Replace 100 μL tips with 200 μL tips
8. Transfer 80 μL Isopropyl Alcohol, 10 μL air gap, and 10 μL Microparticle Solution to the Lysate plate
9. Mix the solution using 10 aspirate/dispense cycles
10.Place the round bottom plate on the magnet plate and allow the microparticles to settle for 10 minutes
11. Transfer all of the solution to waste taking care to not disturb the ring of microparticles
12.Add 170 μL 70% Ethanol allowing to rest for 30 seconds and then transfer to waste
13.Repeat step 12 two more times

Results

The plasmids prepared on the Sciclone were compared with plasmids prepared on Agencourt's DNATrack System. Table 1 shows the sequencing data for each system. The devices compare very closely to each other, indicating the Sciclone procedure is p
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. RNAi for High Throughput Analysis
Post Your Comments:
(Date:12/19/2014)... 18, 2014 With only 2 weeks ... special on Sartorius Biohit products . The product specials ... Picus Electronic Pipette Trade-in Program, and free pipette tips. ... officers around the world. They are ideal for lab ... include:, ,     Full volume range of 0.1 ...
(Date:12/19/2014)... DALLAS , Dec. 18, 2014   ISN ... Health & Safety Performance Indicators publication series. ... hours of activity reported in 2013 within its online ... health and safety key performance indicator (KPI) statistics. ... Hiring Clients, U.S. publication is available in ...
(Date:12/17/2014)... Based on the revenues gained from surgical ... dominated by four major players contributing to approximately ... include GE Healthcare (U.K.), Siemens AG (Germany), Philips ... Report Copy at http://www.marketsandmarkets.com/Market-Reports/surgical-imaging-market-210534462.html , The ... to its strong product portfolio, which includes its ...
(Date:12/17/2014)... 17, 2014 Ipsen Biopharmaceuticals, Inc., ... ADR: IPSEY), today announced that Somatuline® ... Somatuline®) was approved by the U.S. Food and ... neuroendocrine tumors (GEP-NETs) in adult patients with unresectable, ... disease to improve progression-free survival (PFS). , “The ...
Breaking Biology Technology:Major Pipette Distributor Pipette.com Announces Sartorius Year End Specials 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 8
... to return capital to stockholders through liquidationA decisive ... Biotechnology Value Fund, L.P. ("BVF"), today announced that ... voted overwhelmingly to remove the existing Board of ... nominees. The vote took place earlier today at ...
... Revenue To $5.6 Million; 34% Increase in Gross ProfitIDAHO ... Isotopes Inc. (OTC Bulletin Board: INIS) announces the financial ... Total Revenue . Total revenue in ... represents an increase of $911,885 or 19.4%. The ...
... the company,s Annual Report 2008. The report is available ... Bavarian Nordic,s revenue for the year 2008 ... DKK 183 million, which was in line with the ... cash equivalents stood,at DKK 796 million. For 2009, Bavarian ...
Cached Biology Technology:Biotechnology Value Fund, L.P. Announces Overwhelming Support to Remove the Board of Directors of Avigen, Inc. at Special Meeting of Stockholders 2International Isotopes Inc. Announces Year End Financial Results 2International Isotopes Inc. Announces Year End Financial Results 3International Isotopes Inc. Announces Year End Financial Results 4Bavarian Nordic Publishes its Annual Results 2008 2Bavarian Nordic Publishes its Annual Results 2008 3Bavarian Nordic Publishes its Annual Results 2008 4Bavarian Nordic Publishes its Annual Results 2008 5Bavarian Nordic Publishes its Annual Results 2008 6Bavarian Nordic Publishes its Annual Results 2008 7
(Date:12/5/2014)... Utah , Dec. 4, 2014  Tute Genomics, ... raised $2.3 million in Series A1 funding led by ... investors also participated in the investment round. ... the healthcare community adopts next-generation sequencing and seeks new ... said Reid Robison , MD MBA, and CEO ...
(Date:11/21/2014)... YORK , Nov. 19, 2014  Earlier this ... at New York College, and one of the most ... biosensor signals that are transmitted from Smartphones to third ... were Smartphones and has one of the earliest known ... discussing its usage in the military, child care, elder ...
(Date:11/18/2014)... --  News Highlights: , ... Partners Data Lake, an agile data and analytics ... allow researcher and clinicians to explore and develop ... of patients , The Partners Data Lake ... Partners system, breaking down physical barriers for collaboration ...
Breaking Biology News(10 mins):Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 2Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 3Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 4Partners HealthCare and EMC Unite to Solve Health Care's Toughest Challenges Using Big Data 5
... have a soft spot for Botswana, developed while reading ... have had a chance to do any sleuthing of ... when University of Illinois scientist Karen Chapman-Novakofski acquired a ... about the health and nutrition of that country,s elderly. ...
... the University of Leeds have made a breakthrough in ... global disease threats to wild carnivores including lions, African ... discovery of how canine distemper Virus (CDV) jumps across ... a more effective monitoring and control of the virus. ...
... A brightly coloured tropical frog under threat of extinction is ... understand how environment and diet influence its development and behaviour. ... with experts at Chester Zoo in the hope that their ... Cruziohyla calcarifer from extinction in the wild but provide clues ...
Cached Biology News:U of I scientist does nutritional detective work in Botswana 2U of I scientist does nutritional detective work in Botswana 3Research sheds new light on how diseases jump across species 2Frog study takes leaf out of nature's book 2
... yield technology is fast - 2 hour ... gene array analysis , Efficiently ... Kits specific for templates with T7, T3 ... , All kits contain SUPERaseIn Ribonuclease Inhibitor ...
Rad 51C Immunogen: His-tagged human Rad 51C, over-expressed in E. coli Storage: 4 C...
...
...
Biology Products: