Navigation Links
High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

arting Materials:

- DH10B Cells
- 1 Rack of 100 μL tips per sample plate
- 1 Rack of 200 μL tips per sample plate

Sciclone Procedure

Methodology

1. Attach 100 μL tips
2 . Add 95 μL Resuspension Solution and shake @ 1200-1500 rpm for 4 minutes
3. Add 100 μL Lysis Solution and shake @ 800-1000 rpm for 5 minutes
4. Add 100 μL Neutralization Solution and shake @ 800-1000 rpm for 10 minutes
5. Slowly dispense 150 μL of air to gently bubble material away from the tips. This is accomplished by lowering the tips to a height just below the top of the well, then moving back to the 12 o'clock position and lowering the tips to the bottom of the well and dispensing the air
6. After a 3 second pause, aspirate 110 μL of Lysate and transfer to a 96-well round bottom microplate
7. Replace 100 μL tips with 200 μL tips
8. Transfer 80 μL Isopropyl Alcohol, 10 μL air gap, and 10 μL Microparticle Solution to the Lysate plate
9. Mix the solution using 10 aspirate/dispense cycles
10.Place the round bottom plate on the magnet plate and allow the microparticles to settle for 10 minutes
11. Transfer all of the solution to waste taking care to not disturb the ring of microparticles
12.Add 170 μL 70% Ethanol allowing to rest for 30 seconds and then transfer to waste
13.Repeat step 12 two more times

Results

The plasmids prepared on the Sciclone were compared with plasmids prepared on Agencourt's DNATrack System. Table 1 shows the sequencing data for each system. The devices compare very closely to each other, indicating the Sciclone procedure is p
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Highest Possible Transformation Efficiencies for High-Throughput Applications
3. High-Throughput Cultivation of Bacterial Clones
4. High-Throughput Isolation of Total RNA
5. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
6. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
7. High Throughput aRNA Amplification
8. High Throughput siRNA Electroporation
9. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
10. High Throughput RNA Isolation for High Performance Microarray Analysis
11. RNAi for High Throughput Analysis
Post Your Comments:
(Date:7/3/2015)... July 3, 2015 ... verkossa osoitteessa  http://www.openinnovationinscience.at Wienissä ... in Science" -jatkokoulutusohjelmaan 22.7.2015 asti.  ... (LBG) tekemän, kansainvälisiä tutkijoita ja ... kaksi suurinta haastetta ovat kannustimien ...
(Date:7/3/2015)... 3, 2015 Forskare ... 2015 på  http://www.openinnovationinscience.at till det återkommande ... som hålls i Wien ... med internationella forskare och vetenskapsmän som genomfördes ... två största utmaningar bristen på incitament för ...
(Date:7/3/2015)... Forskere kan ansøge ... http://www.openinnovationinscience.at om efteruddannelsesprogrammet "Lab for Open ... Wien .  ... og videnskabsmænd foretaget af Ludwig Boltzmann Gesellschaft ... sundhedsvidenskaben manglen på incitamenter til at undersøge ...
(Date:7/2/2015)... 2015  Spherix Incorporated (Nasdaq: SPEX ) -- an intellectual ... of intellectual property, today announced a litigation update. ... Verizon Services Corp. et al. , Case No. 1:14-cv-00721-GBL-TCB, ... District of Virginia , the Court ... certain key claims in favor of the Defendants on ...
Breaking Biology Technology:Maailman ensimmäinen tiedemiesten koulutukseen suunniteltu avoimen innovaation ohjelma käynnistyy 2Maailman ensimmäinen tiedemiesten koulutukseen suunniteltu avoimen innovaation ohjelma käynnistyy 3Lansering av världens första program för öppen innovation för att utbilda forskare 2Lansering av världens första program för öppen innovation för att utbilda forskare 3Lancering af verdens første åbne innovationsprogram til uddannelse af forskere 2Lancering af verdens første åbne innovationsprogram til uddannelse af forskere 3Spherix Issues A Litigation Update 2Spherix Issues A Litigation Update 3
... Osteotech, Inc. (Nasdaq: OSTE ), a ... regenerative healing, announced today that it has received notification ... granted early termination of the Hart-Scott-Rodino waiting period for ... the merger remains subject to antitrust clearance in foreign ...
... Into New North American... -- SOUDERTON, Pennsylvania, October 13, 2010 ... ... ... var first_result; // Results are keyed by longUrl, so we need to grab the first one. for ...
... ... if (typeof(lpUnit)==,undefined,) var lpUnit=,PRN,; --> ... var shortURL = ""; BitlyCB.alertResponse = function(data) { var s = ... for (var r in data.results) { first_result = data.results[r]; break; } for (var key in first_result) { ...
Cached Biology Technology:Osteotech Announces Early Termination of HSR Waiting Period 2Almac Successfully Moves its Clinical Technologies Business Unit Into New North American Headquarters 2Almac Successfully Moves its Clinical Technologies Business Unit Into New North American Headquarters 3Almac Successfully Moves its Clinical Technologies Business Unit Into New North American Headquarters 4Almac Successfully Moves its Clinical Technologies Business Unit Into New North American Headquarters 5Almac Successfully Moves its Clinical Technologies Business Unit Into New North American Headquarters 6Almac Successfully Moves its Clinical Technologies Business Unit Into New North American Headquarters 7Almac Successfully Moves its Clinical Technologies Business Unit Into New North American Headquarters 8Enerkem Named A Top Global Cleantech Company 2Enerkem Named A Top Global Cleantech Company 3Enerkem Named A Top Global Cleantech Company 4Enerkem Named A Top Global Cleantech Company 5Enerkem Named A Top Global Cleantech Company 6Enerkem Named A Top Global Cleantech Company 7
(Date:6/23/2015)... GARDENS, Fla. , June 23, 2015 ... identity management and authentication solutions, today announced enhanced ... strong, multi-factor authentication solution.  The enhancements build ... use of the DigitalPersona Altus platform and ... reader compatibility. In today,s environment ...
(Date:6/17/2015)... GERMANTOWN, Maryland , June 17, 2015 /PRNewswire/ ...   Produktlinie mit DNA-Tests verbessert den Analyseprozess und senkt ... ; Frankfurt Prime Standard: QIA) hat heute in den ... Investigator ® für die ... neuen Kits zur Erstellung genetischer Fingerabdrücke bieten eine integrierte ...
(Date:6/17/2015)... BEACH GARDENS, Fla. , June 17, 2015 ... management solutions, today announced that its U.are.U ® ... Togo,s Eateries, Inc., a ... fast casual sandwich chain, to increase security, improve ... Crossmatch fingerprint readers enable instant, non-repudiable identity confirmation ...
Breaking Biology News(10 mins):Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3
... demographics likely put you at risk for salt-sensitive hypertension, ... you. The concept is that free radicals in ... rather than eliminate excess through urination. Excess sodium, in ... hypertension researcher in the Section of Experimental Medicine at ...
... and San Diego, Calif. Glioblastoma, the most common and ... treat because the tumors rapidly become resistant to therapy. As ... cell growth and drug resistance, they are discovering molecular pathways ... this deadly cancer. Scientists at the Ludwig Institute for ...
... Oceanography Centre (NOC) and the University of Southampton is ... pressures and low temperatures needed to create hydrate in ... Professors Tim Leighton and Paul White from the University ... have been awarded a grant of 0,8 million by ...
Cached Biology News:Sodium transporter appears likely target for treating salt-sensitive hypertension 2Novel combination therapy shuts down escape route, killing glioblastoma tumor cells 2New tool for measuring frozen gas in ocean floor sediments 2
Goat polyclonal to Duffy / FY / DARC...
... The Centra-CL5R multi-purpose centrifuge offers ... a large assortment of rotor options, ... of laboratory applications., Maximum Versatility , ... 21,000 xg) angle rotors or high-volume (4 ...
... a fully licensed, high throughput, water bath thermal ... to 24 microplates. This equates to 9,216 reactions ... A robotic arm rapidly transfers a basket of ... ramping times that are considerably faster than those ...
Proliferin (C-14)...
Biology Products: