Navigation Links
Genomic DNA Isolation Protocol(1,2,3)

tube containing the deproteinized DNA. Cap the tube tightly.
  • Mix by shaking vigorously enough to form a homogeneous suspension. Do not vortex.
  • Centrifuge at 1500 x g for 5 minutes to isolate the upper, DNA-containing aqueous phase away from the lower, organic solvent phase.
  • Carefully decant upper phase containing the DNA into a fresh, pre-spun, PLG 15 ml Light tube.
  • Repeat steps 1 through 3 but this time extracting with 4 ml water-saturated Phenol-Chloroform (PC, 1:1).
  • Carefully decant upper phase containing the DNA into a clean 15 ml polypropylene centrifuge tube.

    Proceed to Section IV.

  • IV Precipitation of DNA

    At this point, the DNA should have been extracted with both Phenol and Phenol-Chloroform and should be in a clean 15 ml polypropylene screw cap centrifuge tube.

    1. Add 100 l 2 M KCI and mix by gentle inversion.
    2. Overlay DNA solution with 5 ml 95% Ethanol by slowly pipetting the Ethanol down the side of the tube.
    3. Place a Pasteur pipet tip at the interface of the DNA-Ethanol solution and spool the DNA onto the pipet tip by swirling the pipet, keeping the tip at the interface, until the 2 phases are completely mixed.
    4. Place pipet tip with the spooled DNA in 1 ml 70% Ethanol for about 2 minutes.
    5. Remove pipet from the 70% Ethanol and hold upright (tip up) for a few seconds to allow the excess Ethanol to drain away. Do not allow the DNA to dry.
    6. Set pipet tip in a microcentrifuge tube containing 200 l TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0) and incubate f
      '"/>


    Source:


    Page: All 1 2 3 4

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    7. Mouse Tail Genomic DNA Isolation Protocol(1)
    8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    11. Genomic DNA Isolation Protocol(1,2,3)
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Genomic DNA Isolation Protocol

    (Date:9/16/2014)... According to Jeff Howell, Partner of ... Global, new development in Downtown Toronto is expanding at ... another 755 storeys of new development last week (including ... be dominating the Toronto skyline for the foreseeable future ... thirst for new development. , As the Toronto city ...
    (Date:9/16/2014)... September 16, 2014 RENU 28, the world's ... became available for purchase in Australia and New Zealand ... tour beginning September 16th. The tour will make stops in ... Bundaberg on the 18th, Sydney on the 20th, and Melbourne ... Australia and New Zealand and to educate people about the ...
    (Date:9/15/2014)... transitionsthink solid, liquid, and gasbut much stranger ... If phase transitions occur at the coldest ... fluctuations can dramatically transform a material. ... Energy,s Brookhaven National Laboratory and Stony Brook ... absolute zero to isolate and probe these ...
    (Date:9/15/2014)... Md. , Sept. 15, 2014   ... leading innovator of scientific products and services for ... One Acquisition Solution for Integrated Services (OASIS) from ... (FAS). GST was selected to provide the government ... the Small Business (SB) category in Pool 4. ...
    Breaking Biology Technology:ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 2ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 3Global Wellness Company Hosts ASEA Australia Tour September 16-22 2Elusive quantum transformations found near absolute zero 2Elusive quantum transformations found near absolute zero 3Global Science & Technology, Inc. Awarded GSA OASIS Small Business Contract 2Global Science & Technology, Inc. Awarded GSA OASIS Small Business Contract 3
    ... 11:26 a.m. EDT, the space shuttle Atlantis will streak skyward ... last mission. While the STS-135 flight marks the end of ... a new era of research into infectious diseases, thanks to ... Curtiss III, along with their colleagues at Arizona State University,s ...
    ... 2011 By applying a coating to individual silicon ... the materials, efficiency and sensitivity. The findings, published ... Letters , suggest that the coated wires hold promise ... Due to a large surface-to-volume ratio, nanowires typically ...
    ... Phalanx Biotech Group, a global provider of genomic ... expression microarray family – the Rice Genome OneArray®. ... to meet specific isothermal requirements for superb hybridization ... and demonstrates high correlation with qPCR. As opposed ...
    Cached Biology Technology:Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 2Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 3Final countdown: Atlantis to carry next-generation vaccine candidate on last space voyage 4With a simple coating, nanowires show a dramatic increase in efficiency and sensitivity 2Phalanx Biotech Group Launches Rice Genome OneArray® 2
    (Date:9/15/2014)... from digestion to disease resistance. Despite the fact that ... the planet, more is known about belly-button bacteria than ... colleagues working on Panama,s Barro Colorado Island discovered that ... to more than 400 different kinds of bacteria. The ... 7,000 different kinds. , Bacteria in tropical forests may ...
    (Date:9/15/2014)... CAMBRIDGE, Mass. (September 15, 2014) The so-called ... which makes proteinhas long provided a simplified explanation ... in living organisms. , In reality, of ... the schema first articulated nearly 60 years ago ... DNA,s double-helix structure. For one, there are multiple ...
    (Date:9/15/2014)... lowland tapir populations may continue to drop in French ... the Office National de la Chasse et de la ... Global reviewed data retrieved from camera traps in the ... compared this data to current harvest rates in the ... in French Guiana," said Matthias Tobler, a scientist with ...
    Breaking Biology News(10 mins):Smithsonian scientists discover tropical tree microbiome in Panama 2Scientists discover RNA modifications in some unexpected places 2
    ... and fertilizer technology used around the world today were ... scientists and engineers at the Tennessee Valley Authority (TVA) ... TVA official. Shields is now Interim Director of IFDC, ... based in Muscle Shoals, Alabama. "An investment ...
    ... bizarre qualities that improve a creature,s chances of ... separation of populations and the evolution of new ... In the September 2008 issue of Evolution ... examine males from four geographically separated populations of ...
    ... Davis M.I.N.D. Institute and The Children,s Hospital in Denver have ... psychological characteristics of a rare genetic disorder in which males ... normal one of each. The study, published in the June ... Genetics Part A, also offers treatment recommendations for men ...
    Cached Biology News:TVA fertilizer technology used worldwide -- but few new products since 1970s 2TVA fertilizer technology used worldwide -- but few new products since 1970s 3TVA fertilizer technology used worldwide -- but few new products since 1970s 4How 'secondary' sex characters can drive the origin of species 2How 'secondary' sex characters can drive the origin of species 3UC Davis researchers define characteristics, treatment options for XXYY syndrome 2UC Davis researchers define characteristics, treatment options for XXYY syndrome 3
    Goat anti-VAV2 Class: Antibody Product Group: Miscellaneous Antibody...
    ... Mouse monoclonal [F30 P7 D7 ... for all tested applications). ... conjugated to Ovalbumin, corresponding to amino ... Entrez Gene ID: ...
    DAP12 Market Segment: Protein Kinases...
    G-protein coupled purinergic receptor P2Y8...
    Biology Products: