Navigation Links
Genomic DNA Isolation Protocol(1,2,3)

ge tube.
  • Pellet cells by centrifugation at 250 x g for 5 minutes at 4C.
  • Decant supernatant and drain any residual supernatant by inverting the tube on a paper towel for 2 minutes.
  • Suspend cells in 1 ml 1x TBS by repeated up and down pipetting, then add 4 ml 1x TBS and 5 ml 2x Lysis Buffer to the cell suspension and mix by inversion.
  • After a 5 minute incubation on ice, pellet nuclei by centrifugation at 1100 x g for 12 minutes at 4C.
  • Decant supernatant and drain any residual supernatant by inverting the tube on a paper towel for 2 minutes.
    Note: The nuclei may be stored at 70C for several weeks at this point, however, higher molecular weight DNA is obtained from freshly prepared nuclei.

    Proceed to Section II.

  • II Lysis of nuclei and deproteinization of DNA
    1. Suspend pelleted nuclei in 2 ml Saline/EDTA Solution (75 mM NaCl, 24 mM EDTA) by repeated up and down pipetting.
    2. Carefully transfer suspended nuclei to a pre-spun (1500 x g for 23 minutes) PLG 15 ml Light tube.
    3. Add 50 L 20 mg/ml Proteinase K (in 10 mM Tris-Cl, pH 8.0, 1.0 mM CaCl2, 30% Glycerol) and 200 l 10% SDS to the suspended nuclei and mix by gentle inversion.
    4. Incubate for 2 hours at 37C with occasional gentle mixing. Note: This incubation may be extended to 1618 hours if that is more convenient.

      Proceed to Section III.

    III Extraction of protein
    1. Add 4 ml water-saturated Phenol to the PLG 15 ml Light
      '"/>


    Source:


    Page: All 1 2 3 4

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    7. Mouse Tail Genomic DNA Isolation Protocol(1)
    8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    11. Genomic DNA Isolation Protocol(1,2,3)
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Genomic DNA Isolation Protocol

    (Date:1/22/2015)... 22, 2015 GEA Niro Soavi the ... laboratory homogenizer, the PandaPLUS 2000, which is ideal for ... and cell disruption . This compact laboratory homogenizer ... juices, liquid food, food additives and ingredients as well ...
    (Date:1/22/2015)... 22, 2015 Protocol Networks brings independent ... team of brand-neutral, independent consultants, Protocol Networks has recently ... that over the years, his company has attracted several ... of Plainfield, as well as others. With the success ...
    (Date:1/22/2015)... Madison, WI (PRWEB) January 22, 2015 Dr. ... at the 12th annual Scripps Natural Supplements Pre-Conference seminar on ... Supplements Conference is an annual continuing education conference for health ... 15th and included the topic of probiotics in health. Dr. ...
    (Date:1/22/2015)... 22 de enero de 2015  El Dr. Paul ... llamada a nominaciones para 2015. Este prestigioso premio reconoce ... tiene el potencial para hacer, contribuciones destacadas para la ... hasta el 15 de marzo de 2015 a través ...
    Breaking Biology Technology:GEA Niro Soavi Announces the PandaPLUS 2000 Homogenizer for Processing New Applications for Nanoemulsions and Cell Disruption 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4
    ... ANN ARBOR, Mich., Oct. 15 Velcura Therapeutics, ... executive with,more than 20 years of successful regulatory ... Therapeutics President and CEO Michael W. Long, PhD, ... management team. He will be,responsible for our clinical ...
    ... Oct. 15 Luminex Corporation,(Nasdaq: LMNX ) today ... Based Medicine, Inc ("RBM"). As part of the settlement, ... payment was made by RBM,in exchange for resolution of ... as the retirement of Luminex,s stock,ownership in RBM and ...
    ... Oct. 15 The U.S. Food and ... injection) as,a new treatment for complicated intra-abdominal ... has demonstrated,activity against a wide range of ... that cause these serious infections. DORIBAX ...
    Cached Biology Technology:Velcura Therapeutics, Inc. Appoints Industry Executive to Lead Regulatory Affairs 2FDA Approves DORIBAX(TM) for the Treatment of Complicated Intra-Abdominal and Complicated Urinary Tract Infections 2FDA Approves DORIBAX(TM) for the Treatment of Complicated Intra-Abdominal and Complicated Urinary Tract Infections 3FDA Approves DORIBAX(TM) for the Treatment of Complicated Intra-Abdominal and Complicated Urinary Tract Infections 4FDA Approves DORIBAX(TM) for the Treatment of Complicated Intra-Abdominal and Complicated Urinary Tract Infections 5
    (Date:1/22/2015)... N.C. , Jan. 16, 2015  A man-made form ... memory and other mental capabilities in adults with mild ... pilot study led by researchers at Wake Forest Baptist ... adults diagnosed with amnesic mild cognitive impairment (MCI) or ...
    (Date:1/22/2015)... and POINT ROBERTS, Washington , ... source covering leading sectors including technology and tech stocks, releases ... marketplace featuring master pickpocket and security consultant Apollo Robbins ... stolen and talks about the Wocket™ biometric smart wallet, a ...
    (Date:1/22/2015)... , Europe,s most prestigious ... Office to present a video retrospective of the most exciting and ... Christofer Toumazou and Sophie Wilson   A ... Award   Starting on 22 January 2015 on   http://www.epo.org/european-inventor ...
    Breaking Biology News(10 mins):Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3
    ... MCLEAN, Va., Nov. 29, 2012  Northrop Grumman Corporation (NYSE: ... proof of concept for an automated biometric information system ... will be operated for a trial period to enable ... enabled intelligence and to help determine the requirements for ...
    ... William Cooke, a professor in the UTSA College of Education ... selected to serve as the speaker of the Texas Chapter ... Tour. Over the course of a week, Cooke will deliver ... of ACSM,s Texas Chapter. "This is a big honor ...
    ... For the first time in its history, the American Society ... opening session of the ASCB,s Annual Meeting, for "An Evening ... Francisco, on Saturday, Dec. 15. Members of the ... will receive complimentary registration to the ASCB keynote addresses ...
    Cached Biology News:Northrop Grumman Delivers Australian Automated Biometric Information System Trial Proof of Concept 2UTSA physiologist William Cooke named 2013 Lecture Series Speaker by Texas Sports Medicine Chapter 2Public invited to 'An Evening of Science & Art' at ASCB Annual Meeting 2
    ... Many differences exist between the various commercial ... glass slide used, the length and GC ... and the manufacturing protocol employed all influence ... analysis. With all the different variables ...
    ... Purity is >95%NEN Radiolabeled Ligands\n\nReceptor-related research has ... of new radiolabeled ligands selected to keep ... wide range of products and services for ... 400 state-of-the-art radioligands. If you do not ...
    ... The ICS-1500 system provides excellent performance ... pumping, electrolytic suppression, and column heater ... offers outstanding sensitivity and stability. The ... baselines for trace-level work. Operate in ...
    ... SMZ line is a Greenough stereomicroscope designed for ... is available with a choice of a large ... base (N2GG). This microscope can be easily connected ... at the same time giving you ultimate flexibility. ...
    Biology Products: