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Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood ,,, Mini Kit

fficiency of downstream applications such as PCR (see Fig. 2).

Preservative Average
A260/280
Average Concentration
(g/ml)
Average Total Yield (g) 200 l Buffy Coat EDTA 1.77 105.9 19.26 Heparin 1.68 87.88 17.35 Sodium Citrate 1.35 46.9 9.38 200 l Whole Blood EDTA 1.68 31.6 6.32 Heparin 1.63 33.5 6.69 Sodium Citrate 1.6 16.3 5.26 Table 1: Yield and purity data comparing buffy coat and whole blood Fig. 1: gDNA isolated from 200 l of Buffy Coat using the Perfect gDNA Blood Mini Kit.
200 ng DNA on 0.6 % agarose gel in 1x TBE

Lane 1: Lambda Hind III Marker (NEB) Lanes 210: gDNA from buffy coat separated from whole blood preserved with EDTA L
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{3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide}. ,250 ug/ml Propidium Iodide (PI) in PBS....
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