Navigation Links
Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood ,,, Mini Kit

12,00016,000 x g. Carefully remove the spin column without splashing Wash Buffer onto the bottom of the column. Place the spin column into a fresh microcentrifuge tube.
  • Observe the filter membrane to make sure it is dry before proceeding. If the filter looks shiny, spin an additional 1 to 2 minutes before placing the spin column into the fresh microcentrifuge tube.
  • Add 200 l Elution Buffer to the center of the spin column, making sure that the buffer comes into contact with the spin column filter. Incubate the sample at 70C for 3 minutes. Centrifuge for 1 minute at 12,00016,000 x g to elute gDNA. Store purified gDNA at 4C.
  • 1.5 ml of blood:
    The following protocol is intended for the researcher who wants to use only an aliquot of a whole blood sample for isolation of gDNA. An aliquot of the whole blood sample may be removed from the vial using a syringe and placed in a microcentrifuge tube. The blood is usually drawn under vacuum conditions into the vial. It is important that the aliquot of blood removed from the vial is used right away to avoid changing the properties of the blood.
    1. Transfer 1.5 ml of whole blood to a microcentrifuge tube.
    2. Centrifuge tube at 3,000 x g for 10 minutes per microcentrifuge.
    3. Continue with step 3 from the protocol outlined above.
    Gel Electrophoresis:
    200 ng (based on OD reading) was run on a 0.6% agarose gel in 1x TBE. The gel was stained in ethidium bromide. Consistency of bands as well as band size from sample to sample was evaluated.

    PCR:
    g
    '"/>

    Source:


    Page: All 1 2 3 4 5 6 7 8

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    6. Mouse Tail Genomic DNA Isolation Protocol(1)
    7. Genomic DNA Isolation Protocol(1,2,3)
    8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    11. Genomic DNA Isolation Protocol(1,2,3)
    Post Your Comments:
    (Date:10/18/2014)... October 19, 2014 The “Human ... Acting, Intermediate Acting, Premixed), Modern Human Insulin (Rapid ... Levemir, NovoRapid, Novomix, Tresiba, Others) - Forecast to ... drivers, restraints, challenges, opportunities, current market trends, and ... with the estimates and forecasts of the revenue ...
    (Date:10/18/2014)... October 18, 2014 The Asia-Pacific hardware ... market in Asia-Pacific with analysis and forecast of revenue. ... is expected to reach $51,362.6 million by 2018, at ... Browse through the TOC of the Asia-Pacific hardware encryption ... analysis provided. This also provides a glimpse of the ...
    (Date:10/18/2014)... According to a new market research report ... Global Trends & Forecasts up till 2017”, hydraulic fracturing ... This value is expected to increase from $40 billion ... CAGR during the same period. North America is expected ... terms of hydraulic horse power supplied by the year ...
    (Date:10/17/2014)... Oct. 16—As in Alice,s journey through the looking-glass to ... in surprising and unexpected ways, including a new class ... As reported today in The Optical Society,s (OSA) new ... demonstrated, for the first time, a new type of ... instead reflects infrared light by using an unusual magnetic ...
    Breaking Biology Technology:Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 2Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 3Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 4Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 5The Asia-Pacific hardware encryption display market is expected to reach $51,362.6 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific hardware encryption display market is expected to reach $51,362.6 million by 2018 - New Report by MicroMarket Monitor 3Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 2Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 3Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 4Magnetic mirrors enable new technologies by reflecting light in uncanny ways 2Magnetic mirrors enable new technologies by reflecting light in uncanny ways 3Magnetic mirrors enable new technologies by reflecting light in uncanny ways 4
    ... ... PDSS Leadership Award at the 3rd Annual Pacific Drug Safety Summit, which took place last ... the moderator of the event and serves as Chairman of BioSoteria,s Board of Advisors. ... Emeryville, CA (PRWEB) September 30, ...
    ... TOKYO, September 30 Sosei Group Corporation,("Sosei"; TSE ... of the New Drug Application (NDA) for the emergency,contraceptive ... (MHLW) by Sosei Co. Ltd., its wholly owned Japanese ... oral emergency contraceptive "morning after pill" which,is used to ...
    ... N.C., Sept. 29 Oxygen Biotherapeutics, Inc. (OTC ... presentation for investors by company chairman and CEO, ... website. The link to the presentation can be ... Relations section of the website at www.oxybiomed.com. It ...
    Cached Biology Technology:Hugh Tilson Receives Leadership Award at Pacific Drug Safety Summit 2Hugh Tilson Receives Leadership Award at Pacific Drug Safety Summit 3
    (Date:10/14/2014)... 14, 2014 – Scientists from The Scripps Research Institute ... Institutes of Health (NIH) to lead an investigation of ... disease in Africa. The study aims to understand how ... die, while others survive the inflection. , "The ... mechanism of how Lassa fever virus causes disease and ...
    (Date:10/14/2014)... week, an international team of researchers, led by the ... the journal Nature Genetics a brief genomic ... varieties of the tomato plant. , The C.M. Rick ... important role in this study by providing seed of ... This study, which builds on the first tomato genome ...
    (Date:10/14/2014)... Philadelphia, PA, October 14, 2014 – Over the past ... failure of institutions to meet the needs of infants ... failure of these infants to thrive. , ... the development of the cortex. A generation of research ... explore, promote cortical development and cognitive function. In contrast, ...
    Breaking Biology News(10 mins):Scripps Research Institute team receives $6.6 million to investigate deadly Lassa virus 2Institutional rearing may increase risk attention-deficit disorder 2
    ... -- Can,t help molding some snow into a ball and ... lake as you can? New research from Indiana University and ... species on Earth, readily learn to throw long distances. This ... with language development in a way critical to our very ...
    ... discovered the last major element of the series of chemical ... from a protein on the surface of a plant cell ... were already known, new research from a team including Carnegie,s ... about the mechanism through which brassinosteroids cause plant genes to ...
    ... COLUMBIA, Mo. In an article in the Nov. ... National Academy of Science , researchers with Dow AgroSciences and ... when transformed into corn and soybeans, provide robust resistance to ... and soybean growers a solution to the growing problem of ...
    Cached Biology News:IU study: Humans' critical ability to throw long distances aided by an illusion 2IU study: Humans' critical ability to throw long distances aided by an illusion 3Nailing down a crucial plant signaling system 22 bacterial enzymes confer resistanceto common herbicide, say MU researchers 2
    Chicken polyclonal to QPRT ( Abpromise for all tested applications). Antigen: Synthetic peptide, corresponding to Internal sequence amino acids 123-237 of Human QPRT Entrez Gene ID: 23475 ...
    Antibody Diluent for IHC 125 ml...
    QPRT Antibody...
    Mouse monoclonal [4C4] to Y14 ( Abpromise for all tested applications). entrezGeneID: 9939 SwissProtID: Q9Y5S9...
    Biology Products: