Navigation Links
Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood ,,, Mini Kit

  • Centrifuge vial at 1,500 x g for 10 minutes in a clinical centrifuge.
  • Pipette 200 l of buffy coat for each sample to be processed into a fresh microcentrifuge tube.
    The buffy coat is seen as a milky white layer between the clear upper plasma layer and the lower red blood cell layer. The buffy coat layer will be very viscous. Take care when pipetting. Pipetting the buffy coat may be easier if some of the clear plasma layer is removed from the top first.
  • Add 20 l of Proteinase K solution to the microcentrifuge tube. Add 350 l Solution G1 to the same tube.
  • Vortex vigorously for 5 seconds. This step is very important due to the presence of high numbers of white blood cells.
  • Incubate samples in a Thermomixer at 70C, 900 rpm, for 10 minutes. A water bath may be used with intermittent vortexing of the samples if a Thermomixer is not available.
  • Add 200 l Solution G2 to the tube. Vortex vigorously for 5 seconds. Place a spin column in a fresh microcentrifuge tube. Transfer the sample to the spin column assembly by pouring or pipetting. Incubate the sample at room temperature for 1 minute.
  • Centrifuge the sample for 2 minutes at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 600 l Diluted Wash Buffer to the spin column. Centrifuge for 1 minute at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 400 l Diluted Wash Buffer to the spin column. Centrifuge for 3 minutes at
    '"/>


  • Source:


    Page: All 1 2 3 4 5 6 7 8

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    6. Mouse Tail Genomic DNA Isolation Protocol(1)
    7. Genomic DNA Isolation Protocol(1,2,3)
    8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    11. Genomic DNA Isolation Protocol(1,2,3)
    Post Your Comments:
    (Date:11/21/2014)... England , Nov. 21, 2014   TRU-D SmartUVC ... will feature their superbug-killing UVC automated disinfection robot, TRU-D SmartUVC, ... this week from Nov. 23 to 26 at Stand 23. ... is the United Kingdom,s largest infection-related ... infection prevention societies to one of the leading events of ...
    (Date:11/21/2014)... 20, 2014 The California ... research organization representing leading California academic institutions, biotechnology, ... PwC US today released a report revealing ... industry growth. The trend shows a 4 percent ... California Biomedical Industry Report indicates that, while ...
    (Date:11/21/2014)... CA (PRWEB) November 21, 2014 Why ... that makes Albert Einstein so instantly recognizable? Why have ... physicist, mathematician, astronomer and author Hilton Ratcliffe seeks out ... have nothing at all to do with science. In ... Harbor Publishing , November 21, 2014), Ratcliffe puts it ...
    (Date:11/21/2014)... - Aequus Pharmaceuticals Inc. (the "Company" or "Aequus") ... a brokered private placement offering (the "Brokered Offering") ... Securities Inc. and Clarus Securities Inc. acted as ... syndicate of agents that also included Wolverton Securities ... Concurrently with the Brokered Offering, the Company also ...
    Breaking Biology Technology:TRU-D SmartUVC to Showcase Superbug-Killing Robot at FIS 2014 2TRU-D SmartUVC to Showcase Superbug-Killing Robot at FIS 2014 3California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 2California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 3California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 4“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 2“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 3“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 4Aequus Pharmaceuticals closes $4.2 million private placement financing 2Aequus Pharmaceuticals closes $4.2 million private placement financing 3
    ... SAFC Pharma(TM), a focus,area within SAFC(R), a member ... announced that a new reactor at its Arklow ... capacity for large-scale,active pharmaceutical ingredient (API) manufacturing by ... a series of investments designed to,enhance production capacity ...
    ... JOSE, Calif., Sept. 26 Dr. Qi Lu, former EVP ... and serve as the president of HYSTA, USA. HYSTA is ... officially left Yahoo! at end of August, he had served ... both personally and professionally,but one thing that has not changed ...
    ... PIP ), a biodefense company developing medical ... the National,Institute of Allergy and Infectious Diseases (NIAID), ... awarded the Company a multi-year contract,for up to ... generation,recombinant protective antigen (rPA) anthrax vaccine., The ...
    Cached Biology Technology:SAFC Pharma Completes Large-Scale API Manufacturing Expansion in Ireland 2SAFC Pharma Completes Large-Scale API Manufacturing Expansion in Ireland 3Update: HYSTA Announces Dr. Qi Lu to Continue on as President of HYSTA, USA 2PharmAthene Awarded NIAID Contract For Up to $83.9 Million For Third Generation rPA Anthrax Vaccine Program 2PharmAthene Awarded NIAID Contract For Up to $83.9 Million For Third Generation rPA Anthrax Vaccine Program 3PharmAthene Awarded NIAID Contract For Up to $83.9 Million For Third Generation rPA Anthrax Vaccine Program 4PharmAthene Awarded NIAID Contract For Up to $83.9 Million For Third Generation rPA Anthrax Vaccine Program 5PharmAthene Awarded NIAID Contract For Up to $83.9 Million For Third Generation rPA Anthrax Vaccine Program 6
    (Date:11/21/2014)... SAN JOSE, Calif. , Nov. 20, 2014 /PRNewswire/ ... a global leader in microcontroller (MCU) and touch technology ... digital temperature sensors with the widest V ... family delivers higher temperature accuracy and faster I 2 ... nonvolatile registers and serial EEPROM memory making them ideal ...
    (Date:11/18/2014)... , Nov. 18, 2014   EyeLock Inc. ... solutions, and MorphoTrust USA (Safran), a ... today announced a strategic partnership to offer comprehensive ... vehicle administration (MVA), airport screening and financial services ... solutions, MorphoTrust serves consumers through a nationwide network ...
    (Date:11/10/2014)... Laundry detergent pods began appearing on U.S. store shelves ... growing numbers ever since. The small packets can be ... measure out a liquid or powder. The convenience, though, ... new study from researchers at Nationwide Children,s Hospital found ... received reports of 17,230 children younger than 6 years ...
    Breaking Biology News(10 mins):Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 2EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 3Study finds laundry detergent pods, serious poisoning risk for children 2
    ... 12, 2009) German scientist Otto H. Warburg,s theory ... Prize in 1931, but the biochemical basis for his ... from irreversible injury to cellular respiration eventually fell out ... the cause of uncontrolled cell growth. Seventy-eight years ...
    ... Fla. A Mayo Clinic-led international consortium has ... and other neurological disorders. Studying just ... have discovered a genetic defect that results in ... as Perry syndrome. Although this syndrome is exceedingly ...
    ... 12 Industry Leaders Chosen for BOSS-UROCKVILLE, Md., Jan. 8 ... named Lockheed Martin (NYSE: LMT ) an industry ... Operations and Support Services - Unrestricted (BOSS-U) vehicle. As ... selected to participate in this indefinite delivery, indefinite quantity (ID/IQ) ...
    Cached Biology News:Nearly a century later, new findings support Warburg theory of cancer 2Mayo Clinic: Brain disorder suggests common mechanism may underlie many neurodegenerative diseases 2Mayo Clinic: Brain disorder suggests common mechanism may underlie many neurodegenerative diseases 3Mayo Clinic: Brain disorder suggests common mechanism may underlie many neurodegenerative diseases 4Lockheed Martin Team Wins Role on Key Department of Defense Biometrics Contract Vehicle 2
    ... weight determinations for petroleum-based applications. Multiple solvents can be used, such as water, benzene, formamide, ... ... ... ...
    Fast, sensitive quantitation of total MEK 1/2 concentration....
    ... For the ultimate in utility and ... to meet the needs of the researcher. ... interior dimensions or as sheet material that ... enclosures. These isolators are autoclavable and non-cytotoxic ...
    ... Automatic High Sensitivity Laboratory Osmometer for 50 L samples. Operating head automatically rises at the end of ... ... ... ...
    Biology Products: