Navigation Links
Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood ,,, Mini Kit

  • Centrifuge vial at 1,500 x g for 10 minutes in a clinical centrifuge.
  • Pipette 200 l of buffy coat for each sample to be processed into a fresh microcentrifuge tube.
    The buffy coat is seen as a milky white layer between the clear upper plasma layer and the lower red blood cell layer. The buffy coat layer will be very viscous. Take care when pipetting. Pipetting the buffy coat may be easier if some of the clear plasma layer is removed from the top first.
  • Add 20 l of Proteinase K solution to the microcentrifuge tube. Add 350 l Solution G1 to the same tube.
  • Vortex vigorously for 5 seconds. This step is very important due to the presence of high numbers of white blood cells.
  • Incubate samples in a Thermomixer at 70C, 900 rpm, for 10 minutes. A water bath may be used with intermittent vortexing of the samples if a Thermomixer is not available.
  • Add 200 l Solution G2 to the tube. Vortex vigorously for 5 seconds. Place a spin column in a fresh microcentrifuge tube. Transfer the sample to the spin column assembly by pouring or pipetting. Incubate the sample at room temperature for 1 minute.
  • Centrifuge the sample for 2 minutes at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 600 l Diluted Wash Buffer to the spin column. Centrifuge for 1 minute at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 400 l Diluted Wash Buffer to the spin column. Centrifuge for 3 minutes at
    '"/>


  • Source:


    Page: All 1 2 3 4 5 6 7 8

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    6. Mouse Tail Genomic DNA Isolation Protocol(1)
    7. Genomic DNA Isolation Protocol(1,2,3)
    8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    11. Genomic DNA Isolation Protocol(1,2,3)
    Post Your Comments:
    (Date:5/22/2015)... May 22, 2015 Knowledgent , ... paper that explores the potential of big data analytics ... paper was developed to help life sciences and healthcare ... data to gain critical business insights. , The life ... of data driven by patient profiling, compliance and regulatory ...
    (Date:5/21/2015)... 21, 2015 Seventh Wave Laboratories, a ... efficacy of pharmaceutical products and medical devices, announced the ... 63043, a 50,000 sq. ft. building more than three ... strategic growth. Facility renovations will begin immediately and the ... will occur in September. , “We are excited ...
    (Date:5/21/2015)...  Prima Biomed Ltd. (NASDAQ: PBMD ) a ... a leader in the development of immunotherapeutic products for ... CVac data from the Phase II CAN-003 ovarian cancer ... clinically meaningful improvement in Overall Survival ("OS") over standard ... In the group of second remission patients (n=20), the ...
    (Date:5/21/2015)... , May 21, 2015 ... inner feelings - and those of others. Being able ... senses. Analyze and read people to anticipate their thoughts ... your deepest emotions and feelings to create unique abstract ... of responsive games. Get inside a first-person-shooter as it ...
    Breaking Biology Technology:New White Paper Explores the Potential of Big Data Analytics in Life Sciences and Healthcare 2Seventh Wave Laboratories Purchases Building for Expansion, Upcoming Move 2Seventh Wave Laboratories Purchases Building for Expansion, Upcoming Move 3Correction Continues after Big Move - BrokerBank Securities, Inc. 25 Days to Meet ARGUS - The World's First Contactless Emotions Scanner 2
    ... March 3 Collexis Holdings Inc.,(OTC Bulletin Board: ... discovery software, announced today the launch of the ... profiles of 38 institutions and,their more than 60,000 ... clients who are also winners of the Clinical ...
    ... concerted, nationwide effort to,encourage and strengthen collaboration between ... a powerful team for improved patient safety,-- the ... Patient,Compact during Patient Safety Awareness Week, March 8-14, ... http://www.newscom.com/cgi-bin/prnh/20080307/AQF053LOGO ) , The ...
    ... Following the,completion of the Phase II clinical development of ... end of Phase,II meeting with the FDA to discuss ... there was an open and highly constructive discussion with ... the first ever formal discussions with the,agency to license ...
    Cached Biology Technology:Collexis Connects the NIH Clinical and Translational Science Award Programs with CTSA Expert Platform 2Collexis Connects the NIH Clinical and Translational Science Award Programs with CTSA Expert Platform 3NPSF Issues New Universal Patient Compact During Patient Safety Awareness Week 2NPSF Issues New Universal Patient Compact During Patient Safety Awareness Week 3Bavarian Nordic Has Essentially Agreed a Pathway for the Licensure of IMVAMUNE(R) With the FDA After Successful End of Phase II Meeting 2Bavarian Nordic Has Essentially Agreed a Pathway for the Licensure of IMVAMUNE(R) With the FDA After Successful End of Phase II Meeting 3
    (Date:5/20/2015)... DUBLIN , May 20, 2015 ... Markets ( http://www.researchandmarkets.com/research/h8t4x6/5year ) has announced the ... of the Global Iris Recognition Market" ... http://photos.prnewswire.com/prnh/20130307/600769) , ,This research service analyses ... across the government and commercial sectors, ...
    (Date:5/19/2015)... May 19, 2015  Technology is rapidly advancing, outpacing ... in the cloud. Passwords and their management are soon ... and standards-based specifications such as those developed by the ... of passwords presents for BYOD, COPE, IoT, and mobile ... identity protocol. In response to the ...
    (Date:5/14/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/5whpxg/fingerprint ... Sensors Market in Smart Mobile Devices " report ... 2014 was a watershed year for fingerprint sensors in ... Pay,. Apple gave fingerprint sensors a raison d,etre in ... sensors are a must-have feature in flagship smartphones. ...
    Breaking Biology News(10 mins):Global Iris Recognition Market Report 2015 - 5-Year Opportunity Analysis 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2
    ... of the public divided about climate change because they ... more basic science and were more proficient in technical ... study published today online in the journal Nature ... questions is no. Indeed, as members of the public ...
    ... it,s hard to see how a common house sparrow and a ... a bird that weighs less than an ounce, and the other ... and tipped the scales at more than eight tons. ... more closely related than many believed. A new study, led by ...
    ... was associated with higher mortality for eggs and hatchlings of ... worsened by continuing global climate change, according to research published ... . The El Nio Southern Oscillation brought dryer, warmer ... sites in the area. The researchers, led by Pilar Tomillo ...
    Cached Biology News:Yale study concludes public apathy over climate change unrelated to science literacy 2Timing is everything 2Timing is everything 3Timing is everything 4
    Dako Target Retrieval Solution, Citrate pH 6 (x 10) For more information, please visit Dako' web-site. EU regulatory status: CE US regulatory status: IVD...
    ... data acquisition interface. All the functionality of ... processor, increased memory and more available channels ... also inlcudes an option for programmable front-end ... 16 to 48 waveform inputs and from ...
    ... to off-white solid. Increases the expression of glutathione ... in H 2 O. Unstable in aqueous ... EK7713840, CAS 3211-76-5, M.W. 196.1. References: ... and Junod, A.F. 1995. Biochem. J. ...
    ... designed to provide a thin film-like barrier when a ... This barrier creates the proper surface tension to ... the slide. PAP Pen contains a special formulation ... can be removed, if desired, by xylene after the ...
    Biology Products: