Navigation Links
Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood ,,, Mini Kit

eliminate the heme-containing red blood cells. The buffy coat layer is difficult to see and pipette. It is viscous and is present as a thin milky layer above the lower red blood cell layer. Although separating the blood into its components requires an additional step, the benefits of higher gDNA concentration and yield out-weigh the extra effort.

Materials
  • Perfect gDNA Blood Mini Kit
Additional items needed:
  • Ethanol (95100%); pipette tips; Eppendorf microcentrifuge; Eppendorf Thermomixer set at 900 rpm and 70C
  • Whole blood samples from three individuals were collected in Vacutainer (Becton Dickinson tubes) containing three different types of preservatives: EDTA, Sodium Heparin and Sodium Citrate
Method

Several experiments were carried out to validate the performance of the kit when using buffy coat. Volumes ranging from 20 l to 200 l of buffy coat were taken from an entire vial (3 ml) of blood and tested for handling, yield, concentration, and purity as well as downstream applications such as PCR1. The method outlined below provides a step-by-step procedure for separation of buffy coat and isolation of gDNA from either a whole vial of blood or an aliquot of blood.

Entire vial of blood:
The term vial refers to the tubes typically used for collection of the blood. Vacutainer (Becton Dickinson, USA) is an example of this type of vial. These vials are usually glass and may hold various amounts of blood with 1.5 ml being the minimum amount required for this procedure.


    '"/>


Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
6. Mouse Tail Genomic DNA Isolation Protocol(1)
7. Genomic DNA Isolation Protocol(1,2,3)
8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Genomic DNA Isolation Protocol(1,2,3)
Post Your Comments:
(Date:7/29/2015)... ... July 29, 2015 , ... ... supplier, announces the launch of their 2nd generation cell therapy POD® design. The ... CT, but it also represents a new POD® design. , “G-CON first ...
(Date:7/29/2015)... , July 29, 2015  Therapath Neuropathology ( ... increased partnerships with VA and Military Medical centers ... Fiber Density (ENFD) and Sweat Gland Nerve Fiber ... neuropathy ( https://therapath.com/skin ). Demonstration ... Density (ENFD) on punch skin biopsy is a ...
(Date:7/29/2015)... July 29, 2015 /PRNewswire/ - RepliCel Life Sciences Inc. ... medicine company, is pleased to announce that the final ... have been locked.  Prototypes for industrial and regulatory ... an application for CE mark clearance to market ... in 2016. (Photo: http://photos.prnewswire.com/prnh/20150729/248373 )  ...
(Date:7/29/2015)... (PRWEB) , ... July 29, 2015 , ... ... Phytocéane introduces CHRONO NIGHT Express Contouring Bi-Gel, a nighttime-specific product that targets ... amount of sugars that are able to enter fat cells, inhibiting the formation ...
Breaking Biology Technology:G-CON Manufacturing Launches Their 2nd Generation Cell Therapy Cleanroom POD® 2G-CON Manufacturing Launches Their 2nd Generation Cell Therapy Cleanroom POD® 3Neuropathology services for VA and Military health systems 2RepliCel Life Sciences Unveils Innovative Dermal Injector Prototype 2RepliCel Life Sciences Unveils Innovative Dermal Injector Prototype 3RepliCel Life Sciences Unveils Innovative Dermal Injector Prototype 4RepliCel Life Sciences Unveils Innovative Dermal Injector Prototype 5Phytocéane introduces CHRONO NIGHT Express Contouring Bi-Gel 2Phytocéane introduces CHRONO NIGHT Express Contouring Bi-Gel 3
... For 65 years, Mission Pharmacal Company has remained ... to provide the highest quality healthcare medications and treatments ... to be uncertain, this Texas-based company remains steadfast in ... to search for more and better prescription and consumer ...
... Fuels, Inc., (Pink Sheets: HRID ) announced ... CEO and President.  Mr. Cadena was the founder and ... Solutions, and will be instrumental in implementing the Company,s ... Mr. Cadena has over 25 years of extensive ...
... Md., Dec. 6, 2011 GenVec, Inc. (Nasdaq: ... the second milestone in its collaboration with Novartis (NYSE: ... treatments for hearing loss and balance disorders. The milestone ... development activities. "Completing this milestone reflects ...
Cached Biology Technology:Celebrating 65 Years of Providing Superior Healthcare Products, Mission Pharmacal Continues to Thrive 2Celebrating 65 Years of Providing Superior Healthcare Products, Mission Pharmacal Continues to Thrive 3Celebrating 65 Years of Providing Superior Healthcare Products, Mission Pharmacal Continues to Thrive 4Celebrating 65 Years of Providing Superior Healthcare Products, Mission Pharmacal Continues to Thrive 5Celebrating 65 Years of Providing Superior Healthcare Products, Mission Pharmacal Continues to Thrive 6Hybrid Fuels, Inc. Appoints Industry Expert as New CEO 2GenVec Achieves Second Milestone in Collaboration 2GenVec Achieves Second Milestone in Collaboration 3
(Date:7/7/2015)... 2015 Research and ... of the "Capacitive Fingerprint Sensors Patent Landscape" ... this date, fingerprint sensing technology is the most ... are well developed. This patent landscape focuses on ... The domain of capacitive fingerprint sensors is closely ...
(Date:7/2/2015)... 2015 Fingerprint Cards has received an ... FPC1155 from the distributor World Peace Industrial Group (WPI), part ... Asia . Deliveries are planned to take ... used by smartphone manufacturers in Asia . ... communicated revenue guidance of approximately 2,200 MSEK for 2015. ...
(Date:6/25/2015)... , June 25, 2015 ... Market by Type (Swipe & Area), Technology, Material (Optical ... Healthcare, Commercial Security & Others) & Geography - ... marketsandmarkets, the said market is expected to reach ... 17.1%. Browse 76 market Tables ...
Breaking Biology News(10 mins):Worldwide Capacitive Fingerprint Sensors Patent Landscape Market Report 2015-2020 - Main IP Holders include Seiko Epson, AuthenTec, Sony and STMicroelectronics 2FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4
... new type of electrical generator uses bacterial spores to ... research conducted at the Wyss Institute of Biologically Inspired ... driven by changes in humidity from sun-warmed ponds and ... movement of a sheet of rubber coated on one ...
... study by Yale School of Medicine researchers provides insight into ... know about reproductive health. Published in the Jan. 27 issue ... about 50% of reproductive-age women had never discussed their reproductive ... reproductive health provider less than once a year or never. ...
... January 27, 2014 Cancer is the leading ... about 30% of all deaths, according to the Canadian ... effective drugs, it remains extremely difficult to develop anti-cancer ... at how to leverage current experimental therapies, in different ...
Cached Biology News:Getting a charge from changes in humidity 2Getting a charge from changes in humidity 3The science of baby-making still a mystery for many women 2Ottawa researchers discover new combination therapy to kill cancer 2
Rab GDP dissociation inhibitor beta...
... 500V Programmable Power Supply are high-performance, ... handle virtually any application from high ... It is unique in offering both ... POWER Programmable Power Supply is completely ...
Handy 100V Power Supply is Low-cost, compact and lightweight. Easy to operate - either 50 or 100 VDC is selectable as the output voltage. These voltages are sufficient for ordinary electrophoresis....
Coating : Poly-D-Lysine ,Surface : ECM/attachment factors ,Surface area : 9.6 cm2 ,Dim nominal : 35 mm x 10 mm ,Vol working : 2.5-3.0 ml ...
Biology Products: