Navigation Links
General Considerations for Successful Transfection Experiments

establishment of the line is unknown. Different culture conditions could lead to clonal selection. Cell lines with the same name could thus significantly differ with respect to physiology and morphology (and transfectability). Generally, cells are more difficult to transfect the first passage or two out of the freezer. Thereafter, with some cell lines the transfection efficacy remains relatively constant for over 100 passages. With other cell lines, transfection efficacy may either increase or decrease with passage.

Cell Number (Grade of Confluency)
Cell lines divide exponentially when there is space on the substrate (tissue culture dish). The growth rate is negatively affected by cell density (contact inhibition), depletion of nutrients, and metabolic end products (e.g., pH). The extent of reporter gene expression is directly correlated with the cell number at the beginning of transfection and the growth rate prior to cell lysis.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. General Notes on Primer Design in PCR*
2. Real-Time PCR: General Considerations, Rev A
3. Keys to Successful Densitometry
4. Successful PCR amplification and subcloning of a GC-rich DNA fragment
5. Designing a Successful qRT-PCR Experiment
6. Precursor miRNAs for Successful miRNA Functional Studies
7. Recommendations for Successful siRNA Library Screens
8. Setting up Successful siRNA Library Screens
9. Successful stabilization of gene expression profiles
10. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
11. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
Post Your Comments:
(Date:2/27/2015)... The fully automated supercritical fluidic chromatography-based ... analyze up to 48 samples utilizing automatic extraction ... targets by mass spectrometry. , The Nexera ... requirements of a wide range of applications, including ... search for disease biomarkers, additives in forming polymers, ...
(Date:2/27/2015)... Brighton, Illinois (PRWEB) February 27, 2015 ... Matt Sims, General Manager of State Line Farms, received ... Award recognizes a producer, under the age of 35, ... , "It is an honor to win the Tomorrow's ... to discovering who won the Top Producer Award and ...
(Date:2/27/2015)... , Feb. 27, 2015   PureTech , ... inventing and commercializing disruptive products and technologies in the ... Viehbacher , former CEO and Member of the Board ... "It,s has been a pleasure to ... excited that we will be working together more closely ...
(Date:2/26/2015)... 2015  23andMe, the leading personal genetics company, today ... Privacy Officer and Corporate Counsel. Black brings a strong ... well as health care regulations. As a member of ... for reviewing, updating and enhancing the company,s privacy and ... She joined the company January 5, 2015 and reports ...
Breaking Biology Technology:Shimadzu Corporation Launches Nexera UC - The World’s Foremost Unified Chromatography System 2Shimadzu Corporation Launches Nexera UC - The World’s Foremost Unified Chromatography System 3Matt Sims Receives the 2015 Tomorrow’s Top Producer Horizon Award 2Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 2Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 3Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 423andMe Appoints Kate Black as Privacy Officer and Corporate Counsel 2
... University of Wisconsin-Madison Lidar (Light Detection and Ranging) group was ... Land Elevation Satellite) laser device. Lidars Arctic HSRL ... because it operates on the same wavelength as the ICESat. ... climate and changing polar ice sheets in the Arctic. ...
... business is turning around a sick company before all of ... into bankruptcy. Realizing the painful reality of how bad things ... senior executives to deal with truthfully. They grasp at straws ... close that big outstanding proposal, and things will be back ...
... at a double-digit pace in 2003, but there are indications ... year, according to global HR outsourcing and consulting firm Hewitt ... percent average increase for employers, which is lower than 2003's ... absorb the majority of next year's increase, but many are ...
Cached Biology Technology:UW-Madison Laser Device to Study Polar Ice Caps 2Turning Around a Sick Company 2Health Care Costs Continue Double-Digit Pace, But May Start Moderating in 2004 2Health Care Costs Continue Double-Digit Pace, But May Start Moderating in 2004 3
(Date:2/5/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD and ... focused on the growing mobile commerce market, announces it ... Conference on January 29 th at 10:30 a.m. ... of NXT-ID will present and host a live Q&A ... and its full suite of biometric technologies. Investors can ...
(Date:1/22/2015)... S.A. ("Company") (BM&FBOVESPA: VLID3 ON), is pleased to inform its ... of Washington,s Department of Licensing, in the United ... system for driver,s licenses and identification cards to its subsidiary, ... will start in January 2015, with enrollment and card production ...
(Date:1/22/2015)... N.C. , Jan. 16, 2015  A man-made form ... memory and other mental capabilities in adults with mild ... pilot study led by researchers at Wake Forest Baptist ... adults diagnosed with amnesic mild cognitive impairment (MCI) or ...
Breaking Biology News(10 mins):CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 2CEO of NXT-ID, Inc. to Discuss Company's Biometric Technologies at RedChip Online Conference January 29th 3Valid USA Signs Contract For Washington Driver's License Issuance 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3
... the extraction of water for irrigation and overfishing all threaten ... sensitive to changes in its environment and, according to the ... the year 2040 and will have completely disappeared from Iberian ... Global warming is threatening the existence of many fish species, ...
... -- What if you could shut down several emergency ... hacking a pacemaker and reprogramming it to cause a ... an espionage film, they are also some of the ... working to prevent. Eugene Vasserman, assistant professor; John ...
... The Scripps Research Institute have designed a compound that shows ... closely linked to fragile X syndrome, a genetic condition that ... only known single-gene cause of autism. The study, published ... Biology September 4, 2012, focuses on tremor ataxia syndrome, ...
Cached Biology News:Trout will become extinct in the Iberian Peninsula in less than 100 years 2Trout will become extinct in the Iberian Peninsula in less than 100 years 3Internal relations 2Internal relations 3Internal relations 4Scripps Florida scientists design molecule that reverses some fragile X syndrome defects 2
albumin from bovine serum (BSA), tetramethylrhodamine conjugate...
Superior homology search software - in all the flavours of blast, with increased sensitivity and speed...
... Blot Processing mini Tray (3 per ... gel staining and blot processing.Automated staining ... for proteins and nucleic acid analysis.Prepares ... and chromogenic detection.Programmable control of protocol, ...
75 x 25 mm; sterile, recommended for immunohistochemistry of cell lines growing in suspension, Poly-D-Lysine coated, glass slides with polystyrene vessel, lid and safety removal tool...
Biology Products: