Navigation Links
General Considerations for Successful Transfection Experiments

establishment of the line is unknown. Different culture conditions could lead to clonal selection. Cell lines with the same name could thus significantly differ with respect to physiology and morphology (and transfectability). Generally, cells are more difficult to transfect the first passage or two out of the freezer. Thereafter, with some cell lines the transfection efficacy remains relatively constant for over 100 passages. With other cell lines, transfection efficacy may either increase or decrease with passage.

Cell Number (Grade of Confluency)
Cell lines divide exponentially when there is space on the substrate (tissue culture dish). The growth rate is negatively affected by cell density (contact inhibition), depletion of nutrients, and metabolic end products (e.g., pH). The extent of reporter gene expression is directly correlated with the cell number at the beginning of transfection and the growth rate prior to cell lysis.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. General Notes on Primer Design in PCR*
2. Real-Time PCR: General Considerations, Rev A
3. Keys to Successful Densitometry
4. Successful PCR amplification and subcloning of a GC-rich DNA fragment
5. Designing a Successful qRT-PCR Experiment
6. Precursor miRNAs for Successful miRNA Functional Studies
7. Recommendations for Successful siRNA Library Screens
8. Setting up Successful siRNA Library Screens
9. Successful stabilization of gene expression profiles
10. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
11. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
Post Your Comments:
(Date:5/26/2015)... Texas , May 26, 2015  A simple ... Health Science Center to detect early Alzheimer,s disease is ... adults. Detailed standardized guidelines that are needed ... have been published in Alzheimer,s & Dementia . ... of an international working group that includes UNT Health ...
(Date:5/26/2015)... 2015 Leading Regenerative Veterinary Medicine® ... long-term relationship with Veterinary Growth Partners, a membership ... to provide access to vital services to veterinary ... VGP as a dedicated partner in bringing stem ... Concentrating and using stem cells natural abilities to ...
(Date:5/26/2015)... ANGELES , May 26, 2015 Mr. ... his family, thanks President Barack Obama and President ... during the time of loss of Dr. Rongxiang Xu ... humanitarian and a leader ahead of his time, Dr. Xu,s ... the community leave a legacy that will not be forgotten. ...
(Date:5/26/2015)... 26, 2015 Oramed Pharmaceuticals Inc. ... pharmaceutical company focused on the development of oral drug ... study protocol for the company,s Phase IIb trial of ... and Drug Administration (FDA). The submission was made under ... The Phase IIb study of ORMD-0801 for type ...
Breaking Biology Technology:Guidelines bring UNT Health Science Center's blood test for Alzheimer's one step closer 2VetStem Biopharma & Veterinary Growth Partners Join Forces to Increase Member Veterinary Hospital Revenue Through Cutting Edge Medical Offering 2VetStem Biopharma & Veterinary Growth Partners Join Forces to Increase Member Veterinary Hospital Revenue Through Cutting Edge Medical Offering 3Dr. Rongxiang Xu, Beyond A Legend 2Oramed Submits Protocol to U.S. FDA for Its Phase IIb Oral Insulin Study 2Oramed Submits Protocol to U.S. FDA for Its Phase IIb Oral Insulin Study 3
... Open Label Extension Results Support Efficacy and Safety ... Savient,Pharmaceuticals, Inc. (Nasdaq: SVNT ) today announced ... treatment-failure gout (TFG),patients was presented at scientific sessions ... Rheumatology (ACR). Four presentations focused on,results from the ...
... 27 GeneThera, Inc.,(Pink Sheets: GTHR), announced today that ... arm of the University of New Mexico,(UNM), giving GeneThera ... rights to a vaccine developed at UNM,s Health Sciences ... shedding of the,E. coli bacteria in cattle., The ...
... (Nasdaq: NKTR ) announced that it will host ... Hotel in New York on,Wednesday, November 12, 2008, beginning ... event will conclude at approximately 3:30 p.m., Nektar,s ... and Q&A,related to the Company,s clinical development programs, key ...
Cached Biology Technology:Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 2Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 3Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 4Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 5Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 6Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 7Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 8Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 9Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 10Savient's Pegloticase Data in Treatment-Failure Gout Patients Presented at 72nd Annual Meeting of the American College of Rheumatology Conference 11STC.UNM Signs License Option Agreement With GeneThera for E. coli O157:H7 Vaccine 2STC.UNM Signs License Option Agreement With GeneThera for E. coli O157:H7 Vaccine 3STC.UNM Signs License Option Agreement With GeneThera for E. coli O157:H7 Vaccine 4
(Date:5/18/2015)... -- Fingerprint Cards (FPC) has received an order ... FPC1035 from one if its module partners. Deliveries are planned ... and the sensors will be used by smartphone manufacturers in ... hereby amount to 740 MSEK to date. In addition to ... a number of smaller orders not separately communicated amounting to ...
(Date:5/11/2015)... , May 11, 2015  Synaptics Incorporated ... human interface solutions, today announced the appointment of ... and Chief Financial Officer, reporting to Rick ... Synaptics, current Chief Financial Officer, Kathleen Bayless ... Mr. Ali brings extensive financial ...
(Date:5/10/2015)... Fingerprint Cards (FPC) has received an ... the distributor World Peace Industrial Group (WPI), part of WPG ... Asia . Deliveries are planned to take place during ... manufacturers in China . The order value ... of + 1 000 MSEK for 2015.   Jörgen ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 235 Million 2
... even those caused by antibiotic-sensitive bacteria, resist treatment. This ... impossible to cure. A key cause of this ... Starved bacteria resist killing by nearly every type of ... What produces starvation-induced antibiotic resistance, and how ...
... While shorter days and colder weather move many ... spent their summers in fieldwork are more likely to ... samples and crunching data from their summer,s labors. ... spent a month last summer in Mexico,s Sea of ...
... Although scientists have known for centuries that many bacteria produce ... toxic by-product of cellular activity. Now, researchers at NYU ... major role in protecting bacteria from the effects of numerous ... Nudler, PhD, the Julie Wilson Anderson Professor of Biochemistry at ...
Cached Biology News:What bacteria don't know can hurt them 2What bacteria don't know can hurt them 3Squid mystery in Mexican waters unraveled by Stanford biologist and a class of students 2Squid mystery in Mexican waters unraveled by Stanford biologist and a class of students 3Squid mystery in Mexican waters unraveled by Stanford biologist and a class of students 4Targeting bacterial gas defenses allow for increased efficacy of numerous antibiotics 2
... The Microarray Gene Frame® has been ... These frames feature the same dual ... Gene Frame® range for easy removal ... uniformity and there are three sizes ...
Improves spot morphology and DNA binding to the slide resulting in increased hybridization signal. ID Clarifier: Optimized spotting solution for use with SigmaScreen APS Coated Slides for Microarrays...
... easy-to-use solution that enables fully automated hybridization ... Hybridization Station has been designed to offer ... throughput necessary to perform a wide range ... in the range 485C; denaturations of up ...
... The ULS cDNA Synthesis and Labeling Kit ... hybridization experiments. First unmodified cDNA is made ... cDNA using an Oligo(dT) primer. Unlike other ... used in the reverse transcription reaction. This ...
Biology Products: