Navigation Links
Gene Expression Arrays:,,,Highly Sensitive Detection of Expression Patterns,,,with Improved Tools for Target Amplification

seconds, and 72 C for 15 seconds. Fluorescence was monitored at the end of each 55 C incubation. The fluorescence detected in channel F2/F1 was analyzed using the LightCycler Analysis Software. The crossing point for each reaction was determined using the second derivative maximum algorithm and the arithmetic baseline adjustment. For quantification, a titration curve of neomycin mRNA was used.




Results and Discussion
Correlation of results obtained with and without amplification
Total RNA from Saccharomyces cerevisiae grown in rich or minimal medium was labeled with Cy3 and Cy5 by three different methods (Figure 1):
    100 g total RNA by reverse transcription (cDNA labeling/no amplification)
    10 g total RNA by linear amplification (cDNA synthesis followed by in vitro transcription)
    50 ng total RNA by random amplification (random PCR amplification followed by in vitro transcription).
Two hybridizations for each set of probes were performed on the MWG PAN YEAST Array, including a dye swap to minimize normalization and hybridization artifacts [7]. A threshold for low signal intensities was calculated based on the average signal intensities of 15 Arabidopsis-specific negative control spots. The average value of those 15 spots, plus the standard deviation multiplied by 1.96, gives a 95 % confidence that signals above this value are based on specific hybridization. Among a total of 6,250 spots, 75% (4,690) displayed signal intensities above the threshold. For those yeast ORFs, the average ratio of hybridization signals obtained from yeast grown in rich or minimal medium was determined. Within the 4,690 yeast ORFs, 562 ORFs displayed a ratio of ≥2, and 496 ORFs displayed a ratio o
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
(Date:3/27/2015)... 2015  Peter Walter wurde als Gewinner ... seine bahnbrechende Arbeit ausgewählt, die sich damit ... sowie für die Entschlüsselung der Komponenten eines ... nutzen, der mit der Ansammlung von deformierten ... Biochemie an der University of California, San ...
(Date:3/27/2015)... 2015 Working in collaboration with the ... London , Richmond Pharmacology is the first centre ... study for an investigational RNAi therapeutic being developed for the ... nerves and heart. Read press release ... Based at St Georges University of London ...
(Date:3/26/2015)... WARREN, N.J., March 26, 2015  Roka Bioscience, ... company focused on providing advanced testing solutions for ... financial results for the three months and full ... Quarter 2014 Financial Results:Revenue for the quarter ended ... $689,000 for the fourth quarter of 2013 and ...
(Date:3/26/2015)... March 26, 2015  BioNano Genomics, Inc., the leader ... Salyer as chief commercial officer. Salyer has more ... sales teams in life sciences. At BioNano, Salyer will ... a genome-mapping platform to assemble a comprehensive view of ... To date, 32 institutions located in the ...
Breaking Biology Technology:Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 2Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 3Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 4Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 2Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 3Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 4Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 5Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 6Roka Bioscience Reports Fourth Quarter and Full Year 2014 Financial Results 7BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 2BioNano Genomics Appoints Terry Salyer as Chief Commercial Officer 3
... , FREISING-WEIHENSTEPHAN, Germany, September 15 ... a collaboration agreement with,Allergan, Inc. (NYSE: AGN ... in drug delivery and ophthalmic drug,development with a goal ... , Under the terms of ...
... ... use an electric breast pump in public with her new patented device that allows a ... changer for breastfeeding mothers in the workplace, as anchorwoman attests, "It works!" , ... Sacramento, CA (PRWEB) September 15, 2009 -- ...
... , , BALTIMORE, Sept. 14 ... engaged in the development of advanced preclinical platforms and tumor specific ... financial results for the first quarter ended July 31, 2009. Full ... Form 10-Q at www.championsbiotechnology.com . , , ...
Cached Biology Technology:Pieris AG Enters into Anticalin Collaboration With Allergan, Inc. 2ER Doctor Demonstrates Breast Pump on Live TV with Patented New Freemie Hands-Free System 2ER Doctor Demonstrates Breast Pump on Live TV with Patented New Freemie Hands-Free System 3ER Doctor Demonstrates Breast Pump on Live TV with Patented New Freemie Hands-Free System 4Champions Biotechnology Reports Fiscal 2010 First Quarter Financial Results 2Champions Biotechnology Reports Fiscal 2010 First Quarter Financial Results 3Champions Biotechnology Reports Fiscal 2010 First Quarter Financial Results 4Champions Biotechnology Reports Fiscal 2010 First Quarter Financial Results 5
(Date:3/23/2015)... --NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company"), ... market, announces its biometric payment technology, the Wocket® smart wallet, ... March 30 th . The new ... York markets. Gino Pereira ... new ad campaign following the recent initial shipment of Wocket ...
(Date:3/20/2015)... Markets ( http://www.researchandmarkets.com/research/rswjb4/hand_geometry ) has announced the addition ... report to their offering. This report analyzes ... report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... Annual estimates and forecasts are provided for the period 2013 ...
(Date:3/20/2015)... Markets ( http://www.researchandmarkets.com/research/vkzvcs/iris_biometrics ) has announced the addition ... report to their offering. This report analyzes ... report provides separate comprehensive analytics for the US, ... Europe , Asia-Pacific , ... . Annual estimates and forecasts are provided for ...
Breaking Biology News(10 mins):NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 2Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 3Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 4Iris Biometrics - Global Strategic Business Report 2015: System & Network Access Control - A Robust Market Opportunity in the Offing 5
... Prevention and Treatment of Cardiovascular Disease , ... the occasion, the Journal of the American Dietetic ... factors for treating and preventing cardiovascular disease. The ... and other articles provide scientific rationale for food ...
... worlds poorest regions could face severe crop losses in the ... new study by researchers at Stanford Universitys Program on Food ... published in the Feb. 1 issue of the journal Science. ... on agriculture for their livelihoods, said lead author David Lobell, ...
... blood vessels is by creating too much competition for an ... That amino acid, L-arginine, is broken down by the enzyme ... from the proteins we eat. Diabetics have a lot of ... says Dr. Maritza Romero, postdoctoral fellow at the Medical College ...
Cached Biology News:Highlights from the February 2008 Journal of the American Dietetic Association 2Highlights from the February 2008 Journal of the American Dietetic Association 3Impoverished areas of Africa and Asia face severe crop losses from climate change in 20 years 2Impoverished areas of Africa and Asia face severe crop losses from climate change in 20 years 3Impoverished areas of Africa and Asia face severe crop losses from climate change in 20 years 4Diabetes makes it hard for blood vessels to relax 2
... Monoclonal Antibody, Unconjugated Applications: ... ICC: Use at an assay dependent dilution. WB: ... ug/ml, this concentration is determined using a whole ... kDa. Not tested in other applications. Optimal ...
Monoclonal Anti-Gα o purified immunoglobulin buffered aqueous solution Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide. partially purified bovine brain Gα O ...
SHP-2 Antibody...
Anti human PPAR gamma2 mouse monoclonal antibody...
Biology Products: