Navigation Links
Gene Expression Arrays:,,,Highly Sensitive Detection of Expression Patterns,,,with Improved Tools for Target Amplification

seconds, and 72 C for 15 seconds. Fluorescence was monitored at the end of each 55 C incubation. The fluorescence detected in channel F2/F1 was analyzed using the LightCycler Analysis Software. The crossing point for each reaction was determined using the second derivative maximum algorithm and the arithmetic baseline adjustment. For quantification, a titration curve of neomycin mRNA was used.




Results and Discussion
Correlation of results obtained with and without amplification
Total RNA from Saccharomyces cerevisiae grown in rich or minimal medium was labeled with Cy3 and Cy5 by three different methods (Figure 1):
    100 g total RNA by reverse transcription (cDNA labeling/no amplification)
    10 g total RNA by linear amplification (cDNA synthesis followed by in vitro transcription)
    50 ng total RNA by random amplification (random PCR amplification followed by in vitro transcription).
Two hybridizations for each set of probes were performed on the MWG PAN YEAST Array, including a dye swap to minimize normalization and hybridization artifacts [7]. A threshold for low signal intensities was calculated based on the average signal intensities of 15 Arabidopsis-specific negative control spots. The average value of those 15 spots, plus the standard deviation multiplied by 1.96, gives a 95 % confidence that signals above this value are based on specific hybridization. Among a total of 6,250 spots, 75% (4,690) displayed signal intensities above the threshold. For those yeast ORFs, the average ratio of hybridization signals obtained from yeast grown in rich or minimal medium was determined. Within the 4,690 yeast ORFs, 562 ORFs displayed a ratio of ≥2, and 496 ORFs displayed a ratio o
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
(Date:5/22/2015)... 22, 2015 According to a ... & 2D Gel Electrophoresis, Agarose & Polyacrylamide Gel, Capillary ... by Application & by End User - Global Forecast ... expected to reach around $1.98 Billion by 2020 with ... to 2020. Browse 226 ma ...
(Date:5/22/2015)... DALLAS , May 22, 2015 Baylor ... and the Translational Genomics Research Institute (TGen) in ... that will focus on accelerating early detection and treatments for ... science of both institutions will lead to new clinical trials ... therapies – bridging the gap from bench to bedside across ...
(Date:5/21/2015)... May 21, 2015 Specialty Pharmacy ... and been admitted to BPA Worldwide as a ... will track audience data for Specialty Pharmacy Times ... , “By becoming a member of BPA Worldwide, ... providing our clients with the most reliable, unequaled ...
(Date:5/21/2015)... CA (PRWEB) May 21, 2015 ... has announced a partnership with PicnicHealth, a healthcare ... patients. Participants diagnosed with Inflammatory Bowel Disease (IBD) ... as a complementary uBiome research kit. Both companies ... , For more information on ...
Breaking Biology Technology:Electrophoresis Market Worth $1.98 Billion by 2020 2Electrophoresis Market Worth $1.98 Billion by 2020 3Electrophoresis Market Worth $1.98 Billion by 2020 4Precision Medicine Collaboration to Increase Options for Cancer Patients 2Precision Medicine Collaboration to Increase Options for Cancer Patients 3Precision Medicine Collaboration to Increase Options for Cancer Patients 4Specialty Pharmacy Times Joins BPA Worldwide 2uBiome Partners with PicnicHealth 2uBiome Partners with PicnicHealth 3
... 2011 UCB, a leading biopharmaceutical company at the ... 2011 American College of Rheumatology (ACR) meeting by reinforcing ... and their healthcare providers. At this year,s ACR meeting, ... data with new findings on patient-reported quality of life ...
... SQNM ), a life sciences company providing innovative genetic ... third quarter of 2011, an increase of 16% compared to revenues ... for the third quarter of 2011 was $18.4 million or $0.19 ... $0.30 per share for the same period in 2010. ...
... NEW YORK, Nov. 3, 2011 Reportlinker.com announces ... in its catalogue: Global ... http://www.reportlinker.com/p0619270/Global-Biochip-Markets-Microarrays-and-Lab-on-a-Chip.html#utm_source=prnewswire&utm_medium=pr&utm_campaign=NoCategory INTRODUCTION  ... BCC,s goal for this study ...
Cached Biology Technology:UCB Reinforces Long-Standing Commitment to Rheumatology at American College of Rheumatology 2011 Annual Scientific Meeting 2UCB Reinforces Long-Standing Commitment to Rheumatology at American College of Rheumatology 2011 Annual Scientific Meeting 3UCB Reinforces Long-Standing Commitment to Rheumatology at American College of Rheumatology 2011 Annual Scientific Meeting 4Sequenom, Inc. Reports Financial Results for the Third Quarter of 2011 2Sequenom, Inc. Reports Financial Results for the Third Quarter of 2011 3Sequenom, Inc. Reports Financial Results for the Third Quarter of 2011 4Sequenom, Inc. Reports Financial Results for the Third Quarter of 2011 5Sequenom, Inc. Reports Financial Results for the Third Quarter of 2011 6Sequenom, Inc. Reports Financial Results for the Third Quarter of 2011 7Sequenom, Inc. Reports Financial Results for the Third Quarter of 2011 8Global Biochip Markets: Microarrays and Lab-on-a-Chip 2Global Biochip Markets: Microarrays and Lab-on-a-Chip 3Global Biochip Markets: Microarrays and Lab-on-a-Chip 4Global Biochip Markets: Microarrays and Lab-on-a-Chip 5
(Date:5/19/2015)... 19, 2015 Research ... addition of the  "Genetic Testing Market Outlook ... http://photos.prnewswire.com/prnh/20130307/600769) , ,A recent report, Genetic Testing ... of the current and future genetic testing ... their working principles and types are covered ...
(Date:5/14/2015)... 14, 2015 Research and Markets ( ... "Fingerprint Sensors Market in Smart Mobile Devices " ... that 2014 was a watershed year for fingerprint sensors ... ,Apple Pay,. Apple gave fingerprint sensors a raison d,etre ... Fingerprint sensors are a must-have feature in flagship smartphones. ...
(Date:5/11/2015)... 2015  Synaptics Incorporated (NASDAQ: SYNA ), ... announced the appointment of Wajid Ali ... reporting to Rick Bergman , President and ... Officer, Kathleen Bayless , who announced her ... Ali brings extensive financial management expertise to Synaptics, ...
Breaking Biology News(10 mins):Global Genetic Testing Market Outlook 2018 2Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2Synaptics Appoints Wajid Ali as Senior Vice President and Chief Financial Officer 2
... than a decade to understand how tiny molecules called ... that microRNA actually moves between cells to help them ... of cells that grow and develop. This discovery ... including medical gene therapy and bioengineering of crop plants. ...
... simplify lab-on-a-chip devices that could offer quicker, cheaper ... researchers have created microfluidic integrated circuits. Just ... electricity on computer chips without external controls, these ... their devices without instructions from outside systems. ...
... is available in <A HREF=",http://chinese.eurekalert.org/zh/pub_releases/2010-04/sfeb-phr042210.php,">Chinese . ... with extraordinarily complex fluctuations across a wide range of ... both clinical and dynamical significance it may provide ... of the heart,s pacemaking system. In work published ...
Cached Biology News:Production of biofuels could benefit by controlling the types of cells that develop in plants 2Microfluidic integrated circuit could help enable home diagnostic tests 2Microfluidic integrated circuit could help enable home diagnostic tests 3Periodic heart rate decelerations in premature infants 2Periodic heart rate decelerations in premature infants 3
...
Troponin I Immunogen: Full length native protein (purified) (Human). Storage: -20 C, Avoid Freeze/Thaw Cycles...
Anti-SDF-1β/PBSF affinity isolated antibody lyophilized powder Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline recombinant human SDF-1β/PBSF expressed in E....
Immulon 4 HBX 1x12 Strip, flat bottom; high binding extra surface with special resin and irradiation, offers maximum protein uptake....
Biology Products: