Navigation Links
Gene Expression Arrays:,,,Highly Sensitive Detection of Expression Patterns,,,with Improved Tools for Target Amplification

on One microgram of Cy3- and Cy5-labeled cDNA or 10 g cRNA from the respective yeast population was used for hybridization. Hybridizations were performed overnight at 42 C using a buffer containing 50% formamide.

Yeast microarrays
Using a contact printing principle, 5-modified 40-bp oligonucleotides were covalently attached to the solid surface [6]. Each array was comprised of 6,250 oligonucleotides that detected different yeast open reading frames (ORFs).

Image analysis
Scanning for signal intensities was performed with a confocal laser scan microscope. Each slide was scanned six times per channel with an increasing photomultiplier setting to expand the dynamic range of the measurement. Resulting images were analyzed with the Imagene 4.0 Software; the output files were corrected between both channels via total signal intensities and local background subtraction using the MWG MAVI Software.

LightCycler RT-PCR
Quantitative RT-PCR was performed on the LightCycler Instrument using the DNA Hybridization Probe format. cDNA was produced from yeast total RNA in a 20-l reaction, including 20 g total RNA and a defined copy number of neomycin mRNA (spike-in control), oligo dT primer and avian myeloblastosis virus (AMV) reverse transcriptase. RNA and primers were first denaturated at 70 C for 10 minutes, then incubated at 42 C for 60 minutes, followed by a 5-minute denaturation at 94 C. Two hundred picograms and 20 pg of cDNA, primers and hybridization probes, and LightCycler FastStart DNA Master (as described in the protocol) were used in a 20-l reaction. The reaction conditions included: 10 minutes denaturation at 95 C, 45 cycles at 95 C for 10 seconds, 55 C for 15
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
(Date:1/22/2015)... Jan. 22, 2015   Cypher Genomics, Inc., ... (NASDAQ: SQNM ), the leading molecular ... next generation noninvasive prenatal tests (NIPT). Through this ... called Mantis™, to advance analysis of clinically-relevant fetal ...
(Date:12/24/2014)... SILVER SPRING, Md. , Dec. 24, 2014  United ... that Medtronic, Inc. (NYSE: MDT ) has ... and Drug Administration (FDA) for the use of Medtronic,s ... a newly developed catheter) for use with United Therapeutics, ...
(Date:12/24/2014)... GMO corn cases filed across the United States, ... of being consolidated in a Kansas federal court for pretrial ... 162 Corn Litigation, MDL No. 2591 in the U.S. District ... Syngenta GMO corn multidistrict litigation (MDL) has been handed over ...
(Date:12/24/2014)... December 23, 2014 The report ... its definition, classification, application and industry overview. This ... product cost structure. Production is separated by regions, ... raw materials, equipment, downstream client survey, marketing channels, ...
Breaking Biology Technology:Cypher Genomics and Sequenom Announce Development Agreement 2Cypher Genomics and Sequenom Announce Development Agreement 3Cypher Genomics and Sequenom Announce Development Agreement 4United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4Carey Danis & Lowe Reports on Syngenta GMO Corn Transfer Order 2Worldwide Methyl Mercaptan Market 2015-2020 Forecasts on Development & Trends Now Available at ReportsnReports.com 2
... ... 20 Years of Experience to Biochemical Innovator, , ... Houston, TX (PRWEB) February 2, 2010 ... microbiology innovations that produce green chemicals, today announced the appointment of Daniel J. ...
... , EXTON, Pa. , ... Board: FCSC), a biotechnology company focused on the development ... applications, announced today that David Pernock , Chairman ... Executive Officer (CEO), effective immediately. Pernock most recently served ...
... , , ... February 2 Elbit Imaging,(NASDAQ: EMITF ) ("Elbit ... medical holdings (InSightec - 60%, and Gamida,Cell - 28%; both on ... offer shares of such subsidiary in a public offering on the,Tel ...
Cached Biology Technology:Glycos Biotechnologies Expands Management Team with Appointment of Vice President of Research and Development 2Glycos Biotechnologies Expands Management Team with Appointment of Vice President of Research and Development 3Fibrocell Science, Inc. Names David Pernock Chief Executive Officer 2Fibrocell Science, Inc. Names David Pernock Chief Executive Officer 3Elbit Imaging Evaluating Establishment of a Subsidiary to Hold its Bio-Tech and Medical Investments 2Elbit Imaging Evaluating Establishment of a Subsidiary to Hold its Bio-Tech and Medical Investments 3Elbit Imaging Evaluating Establishment of a Subsidiary to Hold its Bio-Tech and Medical Investments 4Elbit Imaging Evaluating Establishment of a Subsidiary to Hold its Bio-Tech and Medical Investments 5
(Date:12/19/2014)... Calif. , Dec. 18, 2014  23andMe, Inc., the leading ... that pinpoints fine-scale differences in genetic ancestry of individuals from ... Since immigrants first arrived more than four hundred years ago, ... a meeting place for peoples from different continents. This study ...
(Date:12/17/2014)... 16, 2014 Valencell, a leader in performance biometric ... to industry leaders such as Intel, Jabra, Atlas, and ... wearable products. These products will be showcased at CES ... Vegas . "Our partners choose ...
(Date:12/11/2014)... DUBLIN , Dec. 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the addition ... report to their offering. One major ... multimodal biometric systems. Multimodal biometric systems utilize more than ...
Breaking Biology News(10 mins):23andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... Dr. Ann F. Jacobson, associate professor in Kent States ... initially choose to postpone but ultimately undergo total knee ... education before and after the procedure. ... post operative experiences with total knee replacement surgery, says ...
... 13, 2008.-Unlike most scientific and technological advances, which tend ... finds itself the center of public debate and regulatory ... many of its applications. , In this second ... first was in 2003), the sample has been enlarged ...
... . , A scientific group of the Universities of ... have deciphered for the first time fruit genomic sequence, in this ... issue of the prestigious journal Nature . One of the ... of the Department of Genetics of the ...
Cached Biology News:Research examines factors in delaying or declining total knee replacement surgery 2BBVA Foundation international study on attitudes to stem cell research 2BBVA Foundation international study on attitudes to stem cell research 3BBVA Foundation international study on attitudes to stem cell research 4BBVA Foundation international study on attitudes to stem cell research 5BBVA Foundation international study on attitudes to stem cell research 6Scientists decipher fruit tree genome for the first time 2Scientists decipher fruit tree genome for the first time 3
Monoclonal Antibody to GLP Immunogen: Ni-NTA purified truncated recombinant GLP expressed in E. Coli strain BL21 (DE3)...
... In Vitro Transcription Kits synthesize RNA probes ... 9 cpm/g. High specific activity, single-stranded ... assays, in situ hybridization, and blot hybridizations. ... the synthesis of modest amounts of unlabeled ...
RAB32, member RAS oncogene family, mRNA (cDNA clone MGC:13500 IMAGE:4280407), complete cds...
Request Info...
Biology Products: