Navigation Links
Gene Expression Arrays:,,,Highly Sensitive Detection of Expression Patterns,,,with Improved Tools for Target Amplification

tissue) are required for expression profiling.

To investigate the bias of these amplification methods, we used yeast as a model eukaryotic organism due to its low level of splicing variants. The experiments included two different cell populations grown in rich and minimal media.

Material and Methods

Sample material
Saccharomyces cerevisiae was grown in YPD medium (50 g/l, rich) and SD base medium (26.7 g/l, minimal). Two percent D+glucose (carbon source) was added to both media. Cells were grown to an OD of 0.4 in a 1 : 4 dilution and collected from 40 ml of cell suspension. Total RNA isolation was performed as described in [5].

Target preparation /amplification

Direct cDNA labeling was performed according to the MWG PAN YEAST Array Application Guide. One hundred microgram of total RNA was labeled in a 40-l labeling reaction by using moloney murine leukemia virus (MMLV) reverse transcriptase and Cy3- and Cy5-dCTP (Amersham Biosciences). Linear amplification was performed with 10 g of starting total yeast RNA according to the package inserts of the cDNA Synthesis System, Microarray Target Purification Kit, and Microarray RNA Target Synthesis Kit (T7), using Cy3- or Cy5-UTP for labeling. Random PCR amplification was performed with 50 ng total yeast RNA according to the package inserts of the Microarray Target Amplification Kit, Microarray Target Purification Kit and Microarray RNA Target Synthesis Kit (T7), using Cy3 or Cy5 for labeling.


Hybridizati
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
(Date:9/19/2014)... 2014 Cereal grain scientists, quality ... gather October 5–8, 2014, for the AACC International ... Center in Providence, Rhode Island. The comprehensive scientific ... food scientists, chemists, microbiologists, nutritionists, and those interested ... management. To date, 1,000 attendees have registered for ...
(Date:9/18/2014)... 2014 Bipartisan passage this week by ... to stimulate development and commercialization of new technologies and ... leaders of SPIE, the international society for optics ... and Innovation (RAMI) Act, now moves to the full ... they are optimistic that it will win passage there ...
(Date:9/18/2014)... 18, 2014 A novel robotic system ... scanner is currently being tested as part of a ... in Boston with the aim of determining if the ... prostate cancer biopsies faster, more accurate, less costly, and ... has the potential to deliver prostate cancer therapies with ...
(Date:9/18/2014)... 2014 On Tuesday, Secretary of Energy ... Risch for National Lab Day on the Hill. The ... national laboratory system. Durbin and Risch also formally launched ... awareness of the reach of the national labs as ... address some of our nation's most pressing challenges. , ...
Breaking Biology Technology:Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 2Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 4Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 5Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 6Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 2Lab Day Highlight Labs' Contribution to U.S. Competitiveness and Innovation 3
... Reportlinker.com announces that a new ... its catalogue. , Worldwide ... Electron Microscope Market Shares, Strategies, and ... , http://www.reportlinker.com/p0153912/Worldwide-Optical-Transmission-TEM-and-Scanning-SEM-Electron-Microscope-Market-Shares-Strategies-and-Forecasts-2009-to-2015.html#utm_source=prnewswire&utm_medium=pr&utm_campaign=prnewswire ...
... (Nasdaq: DNDN ) today announced that management will present at the following ... New York City, Tuesday, November 10, 2009, ... Phoenix, Arizona, Friday, November 13, 2009, ... Healthcare Conference, New York City, Tuesday, November ...
... NeurogesX, Inc. (Nasdaq: NGSX ), a biopharmaceutical ... therapies, announced today that Anthony DiTonno, President and Chief ... conferences in New York City: the Oppenheimer & Co. ... 2009 and the Lazard Capital Markets 6th Annual Healthcare ...
Cached Biology Technology:Reportlinker Adds Worldwide Optical, Transmission TEM, and Scanning SEM Electron Microscope Market Shares, Strategies, and Forecasts, 2009 to 2015 2Reportlinker Adds Worldwide Optical, Transmission TEM, and Scanning SEM Electron Microscope Market Shares, Strategies, and Forecasts, 2009 to 2015 3Reportlinker Adds Worldwide Optical, Transmission TEM, and Scanning SEM Electron Microscope Market Shares, Strategies, and Forecasts, 2009 to 2015 4Reportlinker Adds Worldwide Optical, Transmission TEM, and Scanning SEM Electron Microscope Market Shares, Strategies, and Forecasts, 2009 to 2015 5NeurogesX to Present at Two November Conferences 2NeurogesX to Present at Two November Conferences 3
(Date:9/18/2014)... BUFFALO, N.Y. A sleep-promoting circuit located deep in ... deep sleep. Discovered by researchers at Harvard School of ... and Biomedical Sciences, this is only the second "sleep ... to be both necessary and sufficient to produce deep ... Neuroscience , the study demonstrates that fully half of ...
(Date:9/18/2014)... "frequent flyers" program, fruit flies surely would be eligible ... increasing numbers of fruit fly research studies. One fruit ... and another is scheduled launch to the station Sept. ... December. , Fruit flies are biomedical research models studied ... organisms can reveal the basis for health and disease ...
(Date:9/18/2014)... built the first smartphone app that automatically reveals ... In other words, your smartphone knows your state ... and how that affects you. , The StudentLife ... loneliness to their academic performance, also may be ... to monitor mental health, trigger intervention and improve ...
Breaking Biology News(10 mins):No sedative necessary: Scientists discover new 'sleep node' in the brain 2Buzzing with activity: Fruit flies orbit Earth for science 2Buzzing with activity: Fruit flies orbit Earth for science 3Buzzing with activity: Fruit flies orbit Earth for science 4New Dartmouth smartphone app reveals users' mental health, performance, behavior 2New Dartmouth smartphone app reveals users' mental health, performance, behavior 3New Dartmouth smartphone app reveals users' mental health, performance, behavior 4
... , JUPITER, FL Sathyanaryanan Puthanveettil, an assistant professor ... has been awarded a pair of notable grants to ... Puthanveettil will receive $225,000 over three years from ... long-term memory of a motor protein called kinesin. In ...
... didn,t have enough to worry about from dodging poachers, ... a population of endangered markhora majestic wild goat specieshas ... Wildlife Conservation Society and partners in Tajikistan and France. ... that can reach nearly five feet in length, and ...
... melanoma, the most aggressive skin cancer, is critical because ... of Missouri researchers are one step closer to melanoma ... have a chance to form. Commercial production of a ... will soon be available to scientists and academia for ...
Cached Biology News:Scripps Research Scientist wins pair of grants to study critical component of memory 2Wildlife Conservation Society documents pneumonia outbreak in endangered markhor 2MU researcher's photoacoustic device finds cancer cells before they become tumors 2
Request Info...
... LXQ linear ion trap mass spectrometer enables ... laboratory., The LXQ linear ion trap mass ... proteomic applications, delivering the fast cycle time ... ion trap at an attractive price. ...
... gel stain was developed specifically for reduced mutagenicity ... staining DNA in agarose or acrylamide gels. SYBR ... ethidium bromide but SYBR Safe stain's detection sensitivity ... Safe stain comes as a premixed solution that ...
... are just some the things youll be telling ... Interactions, edited by Erica Golemis, One look at ... compilation of topics in protein interaction will tell ... must be a part of your personal protein ...
Biology Products: