Navigation Links
Gene Expression Arrays:,,,Highly Sensitive Detection of Expression Patterns,,,with Improved Tools for Target Amplification

tissue) are required for expression profiling.

To investigate the bias of these amplification methods, we used yeast as a model eukaryotic organism due to its low level of splicing variants. The experiments included two different cell populations grown in rich and minimal media.

Material and Methods

Sample material
Saccharomyces cerevisiae was grown in YPD medium (50 g/l, rich) and SD base medium (26.7 g/l, minimal). Two percent D+glucose (carbon source) was added to both media. Cells were grown to an OD of 0.4 in a 1 : 4 dilution and collected from 40 ml of cell suspension. Total RNA isolation was performed as described in [5].

Target preparation /amplification

Direct cDNA labeling was performed according to the MWG PAN YEAST Array Application Guide. One hundred microgram of total RNA was labeled in a 40-l labeling reaction by using moloney murine leukemia virus (MMLV) reverse transcriptase and Cy3- and Cy5-dCTP (Amersham Biosciences). Linear amplification was performed with 10 g of starting total yeast RNA according to the package inserts of the cDNA Synthesis System, Microarray Target Purification Kit, and Microarray RNA Target Synthesis Kit (T7), using Cy3- or Cy5-UTP for labeling. Random PCR amplification was performed with 50 ng total yeast RNA according to the package inserts of the Microarray Target Amplification Kit, Microarray Target Purification Kit and Microarray RNA Target Synthesis Kit (T7), using Cy3 or Cy5 for labeling.


Hybridizati
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
(Date:5/26/2015)... May 26, 2015 WORMS, GERMANY ... & Co. (NYSE: GRA) announces that its manufacturing ... practice (GMP) certification from EXCiPACT™, an independent organization ... excipients. , All three Grace facilities ... grade excipient silica gels have now received GMP ...
(Date:5/26/2015)... und HILDEN, Deutschland, May 26, 2015 /PRNewswire/ ... ®   ermöglicht klinischen ... Erstellung dazugehöriger Berichte; Vorstellung auf den Konferenzen ... QIAGEN N.V. (NASDAQ: QGEN ; Frankfurt ... von QIAGEN Clinical Insight ® (QCI™) ...
(Date:5/26/2015)... BioSciences, Inc. (NASDAQ: SGMO ) announced today that ... update on the progress of Sangamo,s ZFP Therapeutic ® ... at 1:30pm ET on Monday, June 1, 2015, at the ... in New York, NY . ... via a link on the Sangamo BioSciences website in the ...
(Date:5/26/2015)... Mass. , May 26, 2015  Alere Inc. (NYSE: ... result of its failure to timely file its Quarterly Report ... 2015 (the "Form 10-Q"), it has received a notice from ... is not in compliance with the NYSE,s continued listing requirements ... the NYSE Listed Company Manual. The reason ...
Breaking Biology Technology:CORRECTION: Grace European Facility Receives GMP Excipient Certification for SYLOID® FP Silica Gel 2QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 2QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 3QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 4QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 5QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 6Sangamo BioSciences Announces Presentation At The Jefferies 2015 Global Healthcare Conference 2
... ISBT 128-compliant label,design and printing software solutions to healthcare providers, blood ... ... 28 Niceware(R) International, LLC is,excited to announce that ICCBBA, Inc. ... bag labeling,software solutions that support ISBT 128-compliant data structures and bar,codes. ...
... With 3.8 Million Patients Under Treatment, -- Diabetes ... ... Shareholders in ... 2008, FRANKLIN LAKES, N.J. and WAKEFIELD, Mass., Aug. 28 ,Medco Health Solutions, Inc. (NYSE: ...
... received in September 2007 for successful completion of ... Phase 2b clinical ... (Nasdaq: HGSI ) today announced that it will receive a ... completion of a Phase 2b trial of Albuferon(R) (albinterferon,alfa-2b) in treatment-naive ...
Cached Biology Technology:Niceware International Announces Licensed Vendor Status with ICCBBA, Inc. to Provide ISBT 128-compliant Software 2Medco Announces Agreement to Acquire Diabetes Care Leader PolyMedica in Transaction Valued at $1.5 Billion 2Medco Announces Agreement to Acquire Diabetes Care Leader PolyMedica in Transaction Valued at $1.5 Billion 3Medco Announces Agreement to Acquire Diabetes Care Leader PolyMedica in Transaction Valued at $1.5 Billion 4Medco Announces Agreement to Acquire Diabetes Care Leader PolyMedica in Transaction Valued at $1.5 Billion 5Medco Announces Agreement to Acquire Diabetes Care Leader PolyMedica in Transaction Valued at $1.5 Billion 6Medco Announces Agreement to Acquire Diabetes Care Leader PolyMedica in Transaction Valued at $1.5 Billion 7Human Genome Sciences Announces $40 Million Milestone Payment Related to Albuferon(R) Development 2Human Genome Sciences Announces $40 Million Milestone Payment Related to Albuferon(R) Development 3Human Genome Sciences Announces $40 Million Milestone Payment Related to Albuferon(R) Development 4
(Date:5/8/2015)... May 8, 2015 Synaptics Inc. (NASDAQ: SYNA ... that members of the executive management team will present at ... Technology, Media and Telecom Conference Date: May 18, 2015 ... Boston, MA Cowen and Company ... 2:45pm ET Location: The New York Palace Hotel, ...
(Date:4/27/2015)... more than four decades, AUVSI,s Unmanned Systems ... event for the unmanned land, sea, and air industries. ... local, national, or trade news organization interested in gathering ... applications of unmanned technologies. The conference will explore the ... and how it will soon impact the lives of ...
(Date:4/20/2015)... 2015 Huntington Memorial Hospital is the first ... new miniaturized, wireless monitoring sensor to manage heart failure ... only FDA-approved heart failure monitoring device that has been ... physicians to manage heart failure. The CardioMEMS ... the pulmonary artery (PA) during a non-surgical procedure to ...
Breaking Biology News(10 mins):Synaptics to Present at Upcoming Investor Conferences 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3
... shows numerous characteristics that are common with a recently identified ... between the ages of 6 and 8. These results ... . Bisphenol A (BPA) is a chemical compound used ... for example to manufacture food containers such as bottles or ...
... dairy organizations from around the world are joining ... dairy at a symposium on "Maintaining health with ... prevention of metabolic syndrome, cardiovascular disease (CVD), obesity ... 20th International Union of Nutritional Science (IUNS) Congress ...
... 10, 2013   EyeLock Inc. , a market leader of iris-based ... Fast IDentity Online Alliance (FIDO) , to spearhead an ... for consumers.  EyeLock will collaborate with Alliance ... and Validity, among others, to identify and set a ...
Cached Biology News:Early exposure to bisphenol A might damage the enamel of teeth 2First international collaboration to showcase latest body of evidence of dairy health benefits 2Iris Biometrics Leader EyeLock Joins the FIDO Alliance to Help Identify More Secure Replacement for Usernames and Passwords 2Iris Biometrics Leader EyeLock Joins the FIDO Alliance to Help Identify More Secure Replacement for Usernames and Passwords 3
... Unique, compact measuring instrument for calibrating single-channel ... design and the robust carrying case, the ... in the laboratory and on-site at the ... to use because the individual channels are ...
... DTT White solid.. PACKAGED UNDER INERT ... so reaction is "driven" to completion. A protective ... surfactant found in many other preparations. Blocks the ... ≥97% by titration. Contaminants: Heavy metals: ...
... Whole Genome ChIP-on-chip Microarray is specifically ... (S. cerevisiae) DNA binding proteins by ... DNA microarrays. This set delivers robust ... greater true binding events and fewer ...
... Basically, most ``special`` dye stains ... a few other non-routine) stains are ... of a paraffin block, staining, coverslipping ... Stain (for senile plaques) ,Bodian`s Stain ...
Biology Products: