Navigation Links
Gene Expression Arrays:,,,Highly Sensitive Detection of Expression Patterns,,,with Improved Tools for Target Amplification

Introduction

As the human genome project is completed, microarray technology offers the potential to study the genomes complexity. This technology facilitates the direct extraction of functional information from nucleic acids by measuring the RNA levels of a complete organism or its associated subsets.

After isolation of total RNA, various methods can be applied to prepare targets for microarray screening (Figure 1). The most common procedure involves direct cDNA labeling by reverse transcription in which fluorescencelabeled nucleotides are incorporated. A clear limitation of this technology is the large amount of RNA required per hybridization. Some of the most important applications in medical research involve studying very small amounts of tissues (e.g., microdissected tissue [LCM] or biopsy material from tumors). Therefore, target amplification methods have been developed to overcome this limitation:

    The linear cRNA amplification procedure, based on reverse transcription with an oligodT primer connected to a T7 promoter and in vitro transcription of resulting DNA with T7 RNA polymerase [1, 2], amplifies a RNA target approximately 100-fold. This method does not significantly distort the relative abundance of individual mRNA sequences within an RNA population [3, 4].
    A PCR amplification method based on reverse transcription, followed by random PCR amplification of the cDNA and in vitro transcription of the resulting PCR product with T7 RNA polymerase, is illustrated in Figure 2. With this procedure as little as 50 ng total RNA (from 1,000 cells or 0.1 mg of
    '"/>


Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
3. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
7. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
8. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
9. A New Lambda Vector for Mammalian Expression
10. Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence
11. Codon Bias-Adjusted BL21 Derivatives for Protein Expression
Post Your Comments:
(Date:10/1/2014)... 2014 The participants committed to not ... spending their own time adjusting, tweaking and testing virtual ... were ready for judging. , “I enjoyed being involved ... leader. “The entries showed a commendable range exploring ... of the print medium and the creativity of the ...
(Date:10/1/2014)... 30, 2014 New applications of structures ... have evolved in nature over millennia are featured in ... for applications in biophotonics in the October issue ... journal is published by SPIE, the international society for ... of the peer-reviewed articles are accessible via open access. ...
(Date:10/1/2014)... 2014 TeselaGen Biotechnology has ... to expand their bioCAD/CAM software suite, which uses ... The Small Business Innovation Research Phase II grant will ... exclusive license from the Lawrence Berkeley National Laboratory. j5 ... an LBNL laboratory that develops alternative fuel technologies for ...
(Date:10/1/2014)... , October 1, 2014 ... software invited to engage around the theme, ,Commercial Opportunities ...   Elsevier , a ... and services, will host a gathering of some of ... and trends impacting healthcare today and in the future. ...
Breaking Biology Technology:Array Architects Challenges Staff to Create with 3D Printer 2Biomimetic and bioinspired materials enable new healthcare options, reports Journal of Biomedical Optics 2Biomimetic and bioinspired materials enable new healthcare options, reports Journal of Biomedical Optics 3TeselaGen Receives $750k NSF SBIR Phase II Award 2TeselaGen Receives $750k NSF SBIR Phase II Award 3Elsevier Healthcare and Pharmaceutical Economics Conference 2014 To Convene Industry Leaders to Discuss Top Healthcare Business Issues 2Elsevier Healthcare and Pharmaceutical Economics Conference 2014 To Convene Industry Leaders to Discuss Top Healthcare Business Issues 3
... Conference Call to be held on May 15, 2008 ... 8:00 am ... April 29 /Xinhua-PRNewswire-FirstCall/ -- 3SBio Inc.,(Nasdaq: SSRX ), ... and marketing biopharmaceutical,products, today announced that it will release ...
... real steps towards building a microscopic device that can ... Writing in the peer-reviewed publication, International Journal of Nanomanufacturing ... a nanoassembler. , In his 1986 book, The Engines ... aim of nanotechnology - to create an assembler, a ...
... Emisphere,Technologies, Inc. (Nasdaq: EMIS ) announced that ... the NASDAQ Stock Market (the "NASDAQ Letter"),advising that, ... Company,s,market value of listed securities had been below ... NASDAQ Global Market pursuant to,NASDAQ Marketplace Rule 4450(b)(1)(A). ...
Cached Biology Technology:3SBio Inc. Schedules 2008 First Quarter Earnings Release on Wednesday, May 14, 2008 2Are nanobots on their way? 2Are nanobots on their way? 3Emisphere Reports Receipt of Notification from NASDAQ Regarding Non-Compliance with Continued Listing Requirements 2Emisphere Reports Receipt of Notification from NASDAQ Regarding Non-Compliance with Continued Listing Requirements 3
(Date:9/30/2014)... the environmental effects of moorland burning is published today ... with the aim of relieving tensions on both sides ... EMBER (Effects of Moorland Burning on the Ecohydrology of ... moorland, which is practised predominantly to support red grouse ... peat hydrology, peat chemistry and physical properties, river water ...
(Date:9/30/2014)... have in common? Unlike most eukaryotic multicellular organisms ... these organisms are all polyploid, meaning they have three ... 3 and 4 sets of chromosomes, respectively, and strawberries ... fact most plant species are polyploid. Polyploidy, or genome ... only recently, with the development of molecular tools, has ...
(Date:9/30/2014)... team of environmental scientists and engineers, led by ... a three-year, $5.25 million grant from the U.S. ... Research to make a direct positive impact on ... and disposal of radioactive contaminants. , "Understanding the ... dealing with environmental aspects of nuclear technologies is ...
Breaking Biology News(10 mins):Grouse moor burning causes widespread environmental changes 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 2Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 3Gene doubling shapes the world: Instant speciation, biodiversity, and the root of our existence 4Researchers get $5.25 million to advance nuclear technologies in South Carolina 2
... image of fossil gliding reptile Mecistrotrachelos apeoros. Curved foot ... of a previously unknown, 220 million-year-old long-necked, gliding reptile ... an industrial-size CT scanner at Penn State's Center for ... small creatures are now known. , The new ...
... at the University of Missouri-Columbia are studying the status ... in the state during the 18th and 19th centuries, ... the late 19th century. The results of the MU ... Conservation to help manage Missouri’s black bear population. , ...
... enough to keep an avian marriage together for the Nazca ... the coast of Ecuador. , Many Nazca booby females switch ... Forest University study scheduled for publication in the journal Proceedings ... , This is surprising because there is an advantage to ...
Cached Biology News:CT scan reveals ancient long-necked gliding reptile 2MU researchers to study the status of black bears in Missouri 2Study investigates 'divorce' among Galapagos seabirds 2
For microscopy...
... that enables fully automated hybridization of microarrays ... has been designed to offer researchers improved ... to perform a wide range of applications. ... range 485C; denaturations of up to 99C. ...
... microarray slide processing offering reproducibility, reliability ... to miniaturization, automation and massive parallel ... tool with many applications in both ... range from gene expression profiling, gene ...
... The user-friendly Eppendorf Thermomixer R offers ... that require shaking, heating, and cooling. ... expanded its application and temperature control ... each accommodate 24 micro test tubes, ...
Biology Products: