Navigation Links
Fluorescence assay for determination of protease activity

In research and industrial work it is quite often necessary to test whether samples or solutions are free of protease activity. Using commercially available kits e.g. from Molecular Probes Inc., protease activity can be measured with the new fluorometer from PerkinElmer Life Sciences, VICTOR.

VICTOR is a new multitechnology and multilabel counter from Wallac that can measure fluorescent, photometric and luminescent labels as well as DELFIA research assays in microtitration plates. It is an epifluorometer that allows the use of both clear and coloured plates depending on the assay requirements.

DESCRIPTION OF ASSAY

In the reaction protease digests a highly quenched BODIPY FL-casein substrate releasing fluorescent peptides. The trypsin used as standard was diluted in 10 mmol/l Tris-HCl buffer, pH 7.8 of which 100 μl was added to the well.

After addition of 100 μl substrate, 10 μg/ml, diluted in 10 mmol/l Tris HCl buffer, pH 7.8, the plate was incubated overnight at room temperature and then measured.

DETECTION

The detection was done using the factory preset settings for fluorescein label. Excitation was at 485 nm and emission was measured at 535 nm.

RESULTS

Measurement with the new VICTOR fluorometer gives results comparable to the data given by the kit manufacturer.

BENEFITS

Fast and simple sample preparation.

PRODUCTS AVAILABLE

1420-011 VICTOR2 multilabel counter

1420-012 VICTOR2 multilabel counter with TR-fluorescence option

1420-014 VICTOR2 basic with stacker and robotic loading system

1420-015 VICTOR2 with TRF with stacker and robotic loading sy stem

1420-041 VICTOR2V with stacker

OTHER PRODUCTS NEEDED

Trypsin enzyme from bovine pancreas, activity 10700 units/mg (Sigma T-8253)

EnzCheck Protease Assay kit from Molecular Probes Inc. (E-6638) Labsystems black Combiplate (cat.no. 95029490)

REFERENCES

Insert of the EnzCheck Protease Assay Kit from Molecular Probes Inc. (E-6638).


'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
2. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
3. Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
4. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
5. Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer
6. Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers
7. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
8. cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
9. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
10. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
11. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... the release of its second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” ... and retention in this eBook by providing practical tips, tools, and strategies for ...
(Date:6/23/2016)... June 23, 2016 Houston Methodist Willowbrook ... Cy-Fair Sports Association to serve as their official ... Houston Methodist Willowbrook will provide sponsorship support, athletic ... with association coaches, volunteers, athletes and families. ... Cy-Fair Sports Association and to bring Houston Methodist ...
(Date:6/23/2016)... ... June 23, 2016 , ... Supplyframe, the Industry Network ... Supplyframe Design Lab . Located in Pasadena, Calif., the Design Lab’s mission is ... projects are designed, built and brought to market. , The Design Lab is ...
(Date:6/23/2016)... Andrew D Zelenetz ... Published recently in Oncology ... touchONCOLOGY, Andrew D Zelenetz , discusses the ... is placing an increasing burden on healthcare systems ... With the patents on many biologics expiring, interest ...
Breaking Biology Technology:
(Date:6/20/2016)... DALLAS , June 20, 2016 ... criminal justice technology solutions for public safety, investigation, ... by the prisons involved, it has secured the ... Corrections (DOC) facilities for Managed Access Systems (MAS) ... (4) additional facilities to be installed by October, ...
(Date:6/15/2016)... New York , June 15, 2016 /PRNewswire/ ... a new market report titled "Gesture Recognition Market by ... and Forecast, 2016 - 2024". According to the report, ... USD 11.60 billion in 2015 and is estimated ... reach USD 48.56 billion by 2024.  ...
(Date:6/9/2016)... , June 9, 2016 ... Police deploy Teleste,s video security solution to ensure the safety ... France during the major tournament Teleste, ... communications systems and services, announced today that its video security ... to back up public safety across the country. ...
Breaking Biology News(10 mins):