Navigation Links
FERMENTATION BASICS

y of the solution.


2.5.2. Gas exchange and mass transfer
One of the most critical factors in the operation of a fermentor is the provision of adequate gas exchange. Oxygen is the most important gaseous substrate for microbial metabolism, and carbon dioxide is the most important gaseous metabolic product.

When oxygen is required as a microbial substrate, it is frequently a limiting factor in fermentation. Because of its low solubility, only 0.3 mM O2, equivalent to 9 mg/l, dissolves in one liter of water at 20 degrees Celsius in an air/water mixture. This amount of oxygen will be depleted in a few seconds by an active and concentrated microbial population unless oxygen is supplied continuously. In contrast, during the same period the amount of other nutrients used is negligible compared to the bulk of concentrations. Therefor most aerobic microbial processes are oxygen limited. This is the reason why the concept of gas-liquid mass transfer in bioprocesses is centered on oxygen transfer even if other gasses such as carbon dioxide, hydrogen, methane and ammonia can also be involved.

Due to the influence of the culture nutrients, the maximal oxygen content is actually lower than it would be in pure water.

The solubility of gasses follows Henry's law in the gas pressure range over which fermentors are operated. This means that if the oxygen concentration in the gas phase increases, the O2 proportion of the nutrient solution increases. Consequently the highest O2 partial pressure are attained during aeration with pure oxygen. Compared to the value in air (9 mg O2/l), 43 mg O2/l dissolves in water when pure oxygen is considered.

As temperature rises, the O2 solubility decreases. For example the solubility at 33 degrees Celcius is
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FERMENTATION BASICS

(Date:7/22/2014)... July 22, 2014 OHAUS Corporation, ... and mechanical balances and scales, proudly announces its ... won Laboratory Equipment magazine’s 2014 “Readers' Choice” Award ... consecutive year the Explorer was voted the best ... Readers’ Choice Awards celebrates “excellence in product design ...
(Date:7/22/2014)... SHENZHEN, China , July 22, ... (BGI Tech), a subsidiary of BGI, the world,s ... of a new human whole exome sequencing service ... offered service includes in-depth bioinformatics analysis and SNP ... December 31, 2014. Complete Genomics, ...
(Date:7/22/2014)... Quorum Review IRB, the industry leader in central ... called Quick Step™ . The offering went live on ... industry’s need for seamless study start-up with a large volume ... planning 50 or more North American investigator sites, as well ... count of 80 or more. Qualifying studies may also enroll ...
(Date:7/22/2014)... Sunnyvale, CA (PRWEB) July 22, 2014 ... begin on September 9th, 2014 and will comprise of ... and Application’ classes. The TechTrainings is a series of ... professionals, designed to help them reach the next step ... SAS class is taught by Jennifer Kang, Senior ...
Breaking Biology Technology:OHAUS Explorer Voted “Best Balance” in Laboratory Equipment’s 2014 Readers’ Choice Awards 2BGI Tech Launches Human Whole Exome Sequencing Service on Complete Genomics' Advanced Platform 2BGI Tech Launches Human Whole Exome Sequencing Service on Complete Genomics' Advanced Platform 3Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 2Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 3Clinovo TechTrainings: Next Session of SAS and CDISC Classes Start 9th September, 2014 2Clinovo TechTrainings: Next Session of SAS and CDISC Classes Start 9th September, 2014 3
... IRVINE, Calif., March 14 Masimo Corporation,(Nasdaq: MASI ), ... pulse oximetry, today announced that it is scheduled,to present at ... The Four Seasons Hotel in Las Vegas, NV on Wednesday, ... and CEO, and Mark P. de,Raad, Executive Vice President and ...
... FRANCISCO, Calif., March 14 ,Renovis, Inc. (Nasdaq: RNVS ... of drugs for major medical needs in the areas ... fourth quarter and year ended December 31, 2007., ... December 31, 2007 was,$1.3 million and $9.8 million, respectively, ...
... Quest Diagnostics,Incorporated (NYSE: DGX ), the ... announced that it is scheduled to speak,at the ... Conference on,Tuesday, March 18, 2008, at the Marriott ... scheduled to begin at,8:45 a.m. Eastern Time., ...
Cached Biology Technology:Masimo to Present at Citi Investment Research 5th Annual Small & Mid-Cap Conference 2Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 2Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 3Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 4Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 5Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 6Renovis Reports Fourth Quarter and Full Year 2007 Financial Results 7Quest Diagnostics to Speak at Cowen and Company 28th Annual Health Care Conference 2
(Date:7/22/2014)... Freezing and thawing might not be good for the ... each fall as they prepare to survive Alaska,s winter ... and thawing outside than a steak does in your ... the spring in better shape than the steak," said ... lead author on a recent paper demonstrating that freeze ...
(Date:7/22/2014)... for Health Policy and Economics have linked tighter ... rates for schizophrenic individuals. , The study comes ... health actually save money, when other costs are ... an extra approval step before tests or treatments ... called prior authorization is intended to encourage ...
(Date:7/22/2014)... light with uncanny precision are key parts of ... manipulate light instead of electricity. But producing these ... with a precise beam of electrons, is often ... Princeton and Columbia universities have proposed a new ... grow these specialized materials, known as photonic crystals, ...
Breaking Biology News(10 mins):Alaska frogs reach record lows in extreme temperature survival 2Alaska frogs reach record lows in extreme temperature survival 3Are state Medicaid policies sentencing people with mental illnesses to prison? 2Technique simplifies the creation of high-tech crystals 2Technique simplifies the creation of high-tech crystals 3
... federal state of Saxony-Anhalt most often suffers from ... by Brandenburg. Hans Hauner of Munich Technical University ... the regional differences in prevalence in general medical ... edition of Deutsches rzteblatt International ( ...
... critical of its life. Having run the gauntlet of air and ... must also evade hungry fish patrolling the beaches in its bid ... cays the risks are high: as many as 30% perish as ... headlong dash through the waves cost the intrepid hatchlings? Curious to ...
... solution to a sticky scientific problem in basic fluid ... even rate, but instead forms what look like fingers ... rivulets "gravity fingers," and the explanation for their formation ... wateror any liquidmeets the soil (or other medium). Knowing ...
Cached Biology News:Cost of hatchling turtles' dash for freedom 2MIT researchers explain mystery of gravity fingers 2
HSV-2 gD (0191)...
...
...
RayBio Human Angiogenesis Antibody Array 2.1 (8 array membranes) with Accessory, detects 23 angiogenic factors (for lysate) Class: Antibody Array Products Product Group: Antibody Array...
Biology Products: