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FERMENTATION BASICS

roorganisms display an optimum pH for growth at around 7, with the majority favoring the pH range 5-8. However, there are exceptions including acetic acid bacteria, thiobacilli and urea decomposing bacteria. In addition numerous algae live in natural waters above pH 10.


2.3. Microbial kinetics
BATCH FERMENTATION

A batch fermentation can be considered to be a closed system. At time t=0 the sterilized nutrient solution in the fermentor is inoculated with microorganisms and incubation is allowed to proceed. In the course of the entire fermentation, nothing is added, except oxygen (in case of aerobic microorganisms), an antifoam agent, and acid or base to control the pH. The composition of the culture medium, the biomass concentration, and the metabolite concentration generally change constantly as a result of the metabolism of the cells.

After the inoculation of a sterile nutrient solution with microorganisms and cultivation under physiological conditions, four typical phases of growth are observed as indicated in Fig.2.


Lag phase
Physicochemical equilibration between microorganism and the environment following inoculation with very little growth.

Log phase
By the end of the lag phase cells have adapted to the new conditions of growth. Growth of the cell mass can now be described quantitatively as a doubling of cell number per unit time for bacteria and yeasts, or a doubling of biomass per unit time for filamentous organisms as fungi. By plotting the number of cells or biomass against time on a semilogarithmic graph, a straight line results, hence the term log phase. Although the cells alter the medium through uptake of substrates a
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