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FERMENTATION BASICS

* Monitoring contamination and process hygiene



Since many bioprocesses, particularly those involving fermentation, are operated batch wise they represent initial-value problems. Consequently process measurement is concerned with setting up the operation accurately and reproducibly. Process control is concerned with making adjustments to the process consequent upon the measurement of one or more of the variables that change as a result of the action of the process. This concerns measuring dependent variables as indicated in Table 5.






Other chemical parameters of interest are substrate concentration and product concentration. These parameters have to be analyzed off-line in the laboratory.

The biological parameters of interest like doubling time, total cell count, cell viability, cell vitality and cell mass dry weight must all be measured outside the fermentor.



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