Navigation Links
FERMENTATION BASICS

is critical because upto 95% of the microorganisms are generally killed during freezing and subsequent thawing. The best method of strain preservation is Lyophilization (freeze-drying).

STAGE 2: GROWTH OF INOCULUM
The preserved culture is initially revived by growth in a erlenmeyer flask on a biological shaker or on a solid medium (if spore formation is needed). In order to obtain sufficient inoculum for small fermentors, a second series of shake cultures is usually made in more flasks. Out from lyophilized strains the growth of inoculum takes around 4-10 days, out from frozen cultures the growth of inoculum takes 4-48 hours for bacteria and 1-7 days for fungi. Finally out of refrigerated cultures the growth of inoculum takes 4-24 hours for bacteria and 1-5 days for fungi.

STAGE 3: FERMENTOR CULTURE
The nutrient media for production must be optimized not only in the ingredients used but also how the medium is prepared and sterilized, pH value before and after sterilization. The most important parameters during the fermentation are:
* Temperature
* Aeration
* Stirring


2.8. Process management
The process management is concerned with:
* Setting up the initial process conditions
* Monitoring to ascertain whether the process is following the required course
* Facilitating manual adjustments to the process variables
* Deciding when to terminate the process and/or to transfer or harvest the product
* Calculating the mass and thermal balances, rates of reaction, kinetics and yields (see Table 4)
* Providing information for statistical records on consistency and for archival purposes

'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FERMENTATION BASICS

(Date:4/21/2015)... April 21, 2015  Novogen Limited (ASX:NRT; NASDAQ: ... advises that it has entered into definitive agreements ... shares plus one attaching 6-month option and half ... share issued, to institutional investors in ... placement for aggregate gross proceeds of approximately AU$15,500,000 ...
(Date:4/21/2015)... PRC Clinical, a Clinical ... the ACRP 2015 Global Conference and Exhibition on April ... of Clinical Research Professionals (ACRP) Global Conference and Exhibition ... on the conduct of clinical trials. ACRP supports clinical ... recognized industry certifications. , The ACRP 2015 Global ...
(Date:4/21/2015)... RESEARCH TRIANGLE PARK, N.C. , April 21, 2015 ... announced today that it will release its first quarter ... 28, 2015. United Therapeutics will host a ... a.m. Eastern Time.  The teleconference is accessible by dialing ... the teleconference will be available for one week and ...
(Date:4/21/2015)... New York (PRWEB) April 21, 2015 ... and Fertility Authority, LLC, announced that it has secured ... Byers (KPCB), TPG Biotech, SR One and Merck Serono ... efforts to provide patients the best fertility outcomes by ... is the largest online marketplace for families seeking fertility ...
Breaking Biology Technology:Novogen Conducts Private Placement and Announces Rights Offering to Shareholders 2Novogen Conducts Private Placement and Announces Rights Offering to Shareholders 3Novogen Conducts Private Placement and Announces Rights Offering to Shareholders 4Novogen Conducts Private Placement and Announces Rights Offering to Shareholders 5Novogen Conducts Private Placement and Announces Rights Offering to Shareholders 6PRC Clinical Sponsors The ACRP 2015 Global Conference And Meets Biotech Companies in the Salt Lake City Area. 2Announcing Progyny: Combining Best-in-Class Clinical Solutions with Consumer Expertise 2
... Holdings, Inc. (Nasdaq: NHWK ), the leading ... United States, today announced that,Tim Mayleben, NightHawk,s President ... for personal reasons. Mayleben will remain with,NightHawk in ... and is,expected to resign from the board of ...
... Martek Biosciences,(Nasdaq: MATK ) announced today that ... eye and heart health, is now available in,two new ... Grapefruit Lychee and Marionberry Guava, join 10 existing flavors ... line is,available at natural food stores nationwide., "We ...
... Board of Directors, Ron ... Vice President Legal Affairs -, SEATTLE, Feb. 13 ... development of therapeutics for,autoimmune and inflammatory disorders, today announced ... of Dendreon Corporation,(Nasdaq: DNDN ), to the Company,s ...
Cached Biology Technology:NightHawk Radiology Holdings, Inc. Announces Senior Executive Departures 2NightHawk Radiology Holdings, Inc. Announces Senior Executive Departures 3Martek's life'sDHA(TM) Now Featured in Two New Flavors of Rachel's(R) Wickedly Delicious Yogurt from WhiteWave Foods 2Martek's life'sDHA(TM) Now Featured in Two New Flavors of Rachel's(R) Wickedly Delicious Yogurt from WhiteWave Foods 3VLST Corporation Announces Board and Executive Management Appointments 2VLST Corporation Announces Board and Executive Management Appointments 3
(Date:4/20/2015)... 2015 Huntington Memorial Hospital is the first ... new miniaturized, wireless monitoring sensor to manage heart failure ... only FDA-approved heart failure monitoring device that has been ... physicians to manage heart failure. The CardioMEMS ... the pulmonary artery (PA) during a non-surgical procedure to ...
(Date:4/20/2015)... 20, 2015 The announcement comes ... Glenbeigh Records Management (GRM), Ireland,s foremost ... Bank. Having built up an impressive track record of clients ... player within the records management sector in Dubai ... is GCC staffbase and employ a further eight staff members ...
(Date:4/17/2015)... , April 17, 2015 ... concerns, and technological advancement to drive biometric systems ... until 2020   According to a ... Biometric Systems Market Forecast &  Opportunities, 2020 ", biometric systems market in ... to register growth at CAGR of over 22% ...
Breaking Biology News(10 mins):Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3
... a room in the intensive care unit (ICU) ... may increase the odds of acquiring such bacteria, ... issue of Archives of Internal Medicine, one of ... significant illness and death in hospitals: methicillin-resistant Staphylococcus ...
... cold seeps--strange clams, mussels and sea snails that thrive in ... marine mollusk community as a whole, according to a new ... published by AAAS, the nonprofit science society. , On average, ... geological record is a full epoch earlier than that of ...
... Boston have demonstrated that it may be possible to ... the heart from scarring. Working with rats, they combined ... division with a naturally occurring growth factor that encourages ... heart-muscle cells to multiply and the heart to regain ...
Cached Biology News:Transmission of antibiotic-resistant bacteria linked to previous intensive care unit room occupants 2Ocean seep mollusks may share evolutionary history with other deep-sea creatures 2Ocean seep mollusks may share evolutionary history with other deep-sea creatures 3Rescuing injured hearts by enhancing regeneration 2Rescuing injured hearts by enhancing regeneration 3