Navigation Links
FERMENTATION BASICS

. On the average, outdoor air has 10-100,000 particles per m3 and 5-2,000 microorganisms per m3. Of these, 50% are fungus spores and 40% are Gram-negative bacteria.

Fermentors generally work with aeration rates of 0.5-2 vvm (air volume/liquid volume per minute). The methods available for sterilizing gases include filtration, gas injection (ozone), gas scrubbing, radiation (UV) and heat. Of these, only filtration and heat are practical.

APPROPRIATE CONSTRUCTION OF THE FERMENTOR
There should be a minimum number of openings in the fermentor to favor maintenance of sterility. Small openings must be made leakproof with O-rings, larger openings with flat gaskets. Whenever a movable shaft penetrates the fermentor wall, special problems of sterility maintenance should be solved.


2.7. Fermentation processes
An overall scheme of a fermentation process can be described as follows:
* Stage 1: inoculum preservation
* Stage 2: inoculum build-up
* Stage 3: fermentor culture

STAGE 1: INOCULUM PRESERVATION
The objective of preservation is to maintain strains as long as possible without cell division. The optimal method of preservation must be worked out for each strain. The following three techniques are most commonly used:
* Storage at low temperatures (2-6 degrees Celcius)
* Frozen storage (-18, -80 or -196 degrees Celcius)
* Lyophilization

Storage at 2-6 degrees Celcius is the least secure, there is a relatively high risk of contamination and reverse mutation through frequent transfer. The frozen storage is the most common and frozen cultures may be kept for several years. The proportion of survivors
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FERMENTATION BASICS

(Date:11/22/2014)... CannLabs, Inc. (OTCQB: CANL), the ... testing methodologies relating to cannabis, today announced that it ... credit from an existing stockholder of the Company. ... commitment from one of our existing stockholders,” stated ... will help accelerate our planned expansions into the Connecticut ...
(Date:11/22/2014)... element of surprise has been a major advantage of ... Until now, epidemiologists had nothing to measure that might ... suboptimal responses to both the H1N1 pandemic of 2009 ... http://photos.prnewswire.com/prnh/20141121/160242 That virus advantage ... structural changes have been found that invariably to date ...
(Date:11/21/2014)... CA (PRWEB) November 20, 2014 Offering ... their Sartorius Entris Balance promotion . The Sartorius ... seeking a durable, high accuracy, and easy-to-maintain balance entry ... in Germany. It was designed to help customer’s bridge ... and a balance that is very durable. Sartorius is ...
(Date:11/21/2014)... BlueInGreen, LLC ( BlueInGreen® ) and ... PCI, Inc., leading suppliers of ozone process technology ... to provide a new state-of-the-art ozone system for ... Wastewater Treatment. The new ozone disinfection system ... dissolution technology, Pinnacle Ozone’s industry leading Zenith™ ozone ...
Breaking Biology Technology:CannLabs Secures $750,000 Line Of Credit 2Viruses' Advantage of Surprise is Lost 2Pipette.com Announces an Extension on the Sartorius Entris Balance Promotion to the End of 2014 2City of Fayetteville Leads Nation with Innovative Ozone Technology 2City of Fayetteville Leads Nation with Innovative Ozone Technology 3City of Fayetteville Leads Nation with Innovative Ozone Technology 4
... May 18 ViroPharma Incorporated (Nasdaq: VPHM ... National Organization for Rare Disorders (NORD) Annual Gala for ... to market for a rare and devastating disorder -- ... genetic disorder caused by a deficiency of C1 inhibitor, ...
... Debt Facility; Support Profitable GrowthALISO VIEJO, Calif., May 18 ... CLRT ), a premier anatomic pathology and molecular ... industry, announced today that it has completed the second ... with Oak Investment Partners , a ...
... and Merieux Alliance,have signed a letter of intent for ... The Institut Pasteur and Merieux Alliance (which comprises the ... to,pool their resources and efforts to fight infectious diseases ... - Clinical diagnostics and theranostics (in ...
Cached Biology Technology:ViroPharma Incorporated Honored by the National Organization for Rare Disorders 2ViroPharma Incorporated Honored by the National Organization for Rare Disorders 3ViroPharma Incorporated Honored by the National Organization for Rare Disorders 4ViroPharma Incorporated Honored by the National Organization for Rare Disorders 5Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 2Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 3Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 4Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 5Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 6The Institut Pasteur and Merieux Alliance Take First Step Towards a Long-Term Research Partnership in the Field of Infectious Diseases 2The Institut Pasteur and Merieux Alliance Take First Step Towards a Long-Term Research Partnership in the Field of Infectious Diseases 3
(Date:11/5/2014)... diversity in their ability to identify scents and odors. ... their perceptual evaluation of odors, with women outperforming men ... Sex differences in olfactory detection may play a role ... one,s perception of smell, which is naturally linked to ... been suggested to be cognitive or emotional, rather than ...
(Date:11/4/2014)... the right amount of death at the right time ... to new research that could help in understanding animal ... , In a paper in the journal Trends ... and European colleagues conclude that the kind of positive ... of individuals, or mortality, depends on the size and ...
(Date:11/4/2014)... GABLES, Fla. (November 4, 2014) — Think about the ... ask: How do neighboring cells know that they are ... and how do these tissues find the correct place ... are answering these crucial questions. , In a new ... use to communicate with their surrounding neighbors, at the ...
Breaking Biology News(10 mins):The female nose always knows: Do women have more olfactory neurons? 2When less is more: Death in moderation boosts population density in nature 2The inside story: How the brain and skull stay together 2
... Larry Murdoch have received an $891,000 Environmental Protection Agency ... in geological formations located deep below the earth,s surface. ... believed to be the leading cause of global warming, ... most promising alternatives for reducing emissions using current technology. ...
... . The DFG has approved the establishment of ... decision was made recently by the relevant Grants Committee in Bonn. ... a total of 132 million. In addition, 20 percent overhead funding ... centres, research topics range from modern prostheses to political reforms to ...
... Journal of Experimental Biology are pleased to announce ... Scientifique (CNRS) in the Universit de Nice-Sophia-Antipolis is the winner ... prize is awarded in recognition of an outstanding achievement and ... JEB Editor-in-Chief, and adds that ,we truly appreciate ...
Cached Biology News:DFG approves funding for 17 new collaborative research centers 2DFG approves funding for 17 new collaborative research centers 3DFG approves funding for 17 new collaborative research centers 4DFG approves funding for 17 new collaborative research centers 5DFG approves funding for 17 new collaborative research centers 6DFG approves funding for 17 new collaborative research centers 7DFG approves funding for 17 new collaborative research centers 8DFG approves funding for 17 new collaborative research centers 9French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 2French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 3French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 4
Alexa Fluor 647 anti-mouse IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40 25 ug...
MAb to Transferrin Dry Ice: No Preservative: NaN3 Availability When Not in Inventory: 14-21 Business Days...
Rabbit polyclonal to IRS1 (phospho S636) ( Abpromise for all tested applications). entrezGeneID: 3667 SwissProtID: P35568...
Leucine-rich repeat-containing G-protein coupled receptor 4...
Biology Products: