Navigation Links
FERMENTATION BASICS

. On the average, outdoor air has 10-100,000 particles per m3 and 5-2,000 microorganisms per m3. Of these, 50% are fungus spores and 40% are Gram-negative bacteria.

Fermentors generally work with aeration rates of 0.5-2 vvm (air volume/liquid volume per minute). The methods available for sterilizing gases include filtration, gas injection (ozone), gas scrubbing, radiation (UV) and heat. Of these, only filtration and heat are practical.

APPROPRIATE CONSTRUCTION OF THE FERMENTOR
There should be a minimum number of openings in the fermentor to favor maintenance of sterility. Small openings must be made leakproof with O-rings, larger openings with flat gaskets. Whenever a movable shaft penetrates the fermentor wall, special problems of sterility maintenance should be solved.


2.7. Fermentation processes
An overall scheme of a fermentation process can be described as follows:
* Stage 1: inoculum preservation
* Stage 2: inoculum build-up
* Stage 3: fermentor culture

STAGE 1: INOCULUM PRESERVATION
The objective of preservation is to maintain strains as long as possible without cell division. The optimal method of preservation must be worked out for each strain. The following three techniques are most commonly used:
* Storage at low temperatures (2-6 degrees Celcius)
* Frozen storage (-18, -80 or -196 degrees Celcius)
* Lyophilization

Storage at 2-6 degrees Celcius is the least secure, there is a relatively high risk of contamination and reverse mutation through frequent transfer. The frozen storage is the most common and frozen cultures may be kept for several years. The proportion of survivors
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FERMENTATION BASICS

(Date:7/6/2015)... Wellesley, Mass. (PRWEB) , ... July 06, 2015 ... ... development of simpler and cheaper molecular diagnostic tests are key drivers of the ... projected growth of personalized medicine and the introduction of new companion diagnostic tests ...
(Date:7/6/2015)... 6, 2015 Navitas, a leading Regulatory Service provider, today announced ... hub in Bogota, Colombia . Navitas, expansion into ... provided by Colombia , the third largest economy in ... Logo - http://photos.prnewswire.com/prnh/20150703/228239LOGO ... based clients to communicate with Navitas regulatory professionals in close to or ...
(Date:7/3/2015)... ... July 03, 2015 , ... ... for use in applications such as animal waste reduction, bioremediation, wastewater treatment, ... restaurant kitchen settings, announced today that it will be showcasing, ManureMagicâ„¢, its ...
(Date:7/3/2015)... ... 03, 2015 , ... The global failure analysis market is expected to reach ... bioscience market is expected to grow at the highest CAGR of 8.46%; its growth ... the research labs and academic institutions for root cause analysis of failure. The market ...
Breaking Biology Technology:Demand for Personalized Medicine, Innovative Molecular Diagnostic Tests Spurring Big Growth, According to BCC Research 2Demand for Personalized Medicine, Innovative Molecular Diagnostic Tests Spurring Big Growth, According to BCC Research 3Navitas Launches Latin American Regulatory Operations Hub in Bogota, Colombia 2Navitas Launches Latin American Regulatory Operations Hub in Bogota, Colombia 3DryLet to Highlight Benefits of Patented ManureMagicâ„¢ at North American Manure Expo in Chambersburg, PA July 14-15, 2015 2Failure Analysis Market to Grow at 7.52% CAGR to 2020 2Failure Analysis Market to Grow at 7.52% CAGR to 2020 3Failure Analysis Market to Grow at 7.52% CAGR to 2020 4Failure Analysis Market to Grow at 7.52% CAGR to 2020 5
... March 28, 2012  Regado Biosciences, Inc., a privately held ... control agents, announced that David J. Mazzo, PhD, President ... 11th Annual Healthcare Conference at 10 a.m. EDT on ... in New York City. The presentation will ...
... little dry ice and a simple industrial process cheaply ... Case Western Reserve University report. Graphene, which ... in pencils, has been hailed as the most important ... than copper, heat better than any material known, are ...
... March 26, 2012 Aviir, a biotechnology company dedicated ... tests and Guard A Heart, a non-profit foundation committed ... Storyland, a Red Carpet Charity Extravaganza in Hollywood on ... whimsical Dr. Seuss theme, as homage to the children ...
Cached Biology Technology:Regado Biosciences, Inc. to Present at Needham & Company's 11th Annual Healthcare Conference in New York, NY 2Regado Biosciences, Inc. to Present at Needham & Company's 11th Annual Healthcare Conference in New York, NY 3Simple, cheap way to mass-produce graphene nanosheets 2Aviir Diagnostics Laboratory in Collaboration with Cause;ology Sponsors "Storyland," a Red Carpet Charity Extravaganza in Hollywood 2Aviir Diagnostics Laboratory in Collaboration with Cause;ology Sponsors "Storyland," a Red Carpet Charity Extravaganza in Hollywood 3
(Date:6/25/2015)... 25, 2015 According to ... (Swipe & Area), Technology, Material (Optical Prism, Piezoelectric, ... Security & Others) & Geography - Global Forecast ... said market is expected to reach $14,500.07 Million ... Browse 76 market Tables and 109 ...
(Date:6/25/2015)... , June 25, 2015  TAKE Solutions Ltd., ... patent by the United States Patent and Trademark Office ... This process leverages TAKE Solutions, Clinical Accelerators to reduce ... 50% (when compared to standardization without the accelerators), thus ... At the heart of the ...
(Date:6/24/2015)... 24, 2015 This report provides market analysis ... six years. It contains an analysis of the drivers, ... their impact from the short, medium, and long term ... industry, market, and technology trends that currently, prevail in ... concerned authorities to efficiently manage the increasing marine traffic ...
Breaking Biology News(10 mins):Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4TAKE Solutions Awarded Patent By USPTO 2Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2
... new species of bird in Gabon, Africa, that was, ... were published in the international science journal Zootaxa today, ... (Stiphrornis pyrrholaemus) was named by the scientists for its ... inches in length and average 18 grams in weight. ...
... A new systematic analysis of the relationship between ... of trends in cancer gene expression. The research, ... Genome Biology, describes how cancers can be divided ... Isaac S Kohane from Children,s Hospital Boston ...
... 14 L-1 Identity,Solutions, Inc. (NYSE: ID ), ... that it closed its tender offer for all of ... and completed its,acquisition of Digimarc,s ID systems business., ... expired at,5:00 p.m. (ET) on August 13, 2008. Through ...
Cached Biology News:Smithsonian scientists discover new bird species 2Smithsonian scientists discover new bird species 3Cancer signatures uncovered 2L-1 Identity Solutions Completes Acquisition of the ID Systems Business from Digimarc Corporation 2L-1 Identity Solutions Completes Acquisition of the ID Systems Business from Digimarc Corporation 3
... The iEMS Incubator/Shaker HT ... Thus, the iEMS Incubator/Shaker HT ... as DNA hybridization and primer ... is available as a one-cabinet ...
The iEMS Incubator/Shaker is a high performance microplate incubator and orbital shaker. Its superior temperature control and efficient orbital shaking reduce incubation times. It can incubate up to ...
... nucleotide structure called a ?cap? is ... naturally-occurring eukaryotic mRNAs and many viral ... a portion of the GTP present ... results in the synthesis of transcripts ...
... nucleotide structure called a ?cap? is ... naturally-occurring eukaryotic mRNAs and many viral ... a portion of the GTP present ... results in the synthesis of transcripts ...
Biology Products: