Navigation Links
FERMENTATION BASICS

of spores, temperatures of 121 degrees Celcius are needed during 15 minutes.

During heat sterilization there is always the possibility of destroying ingredients in the medium. Apart from the degradation of heat-labile components, also contributes to the loss of nutrient quality during sterilization. A common phenomenon is the occurance of the Maillard-type browing reactions which cause discoloration of the medium as well as loss of nutrient quality. These reactions are normally caused by carbonyl groups, usually from reducing sugars, interacting with amino groups from amino acids and proteins. Separate sterilization of the carbohydrate component of the medium may be necessary to prevent such reactions.

Filter sterilization is often used for all components of nutrient solutions which are heat sensitive. Sugars, vitamins, antibiotics or blood components are examples of heat-labile components which must be sterilized by filtration.

Most nutrient media are presently sterilized in batch volumes in the bioreactor at 121 degrees Celcius. Approximate sterilization times can be calculated from the nature of the medium and the size of the fermentor. Not only the nutrient media, but also the fittings, valves and electrodes of the fermentor itself must be sterilized. Therefor, actual sterilization times are significantly longer than calculated ones and must be empirically determined for the specific nutrient solutions in the fermentor. Smaller fermentors are sterilized in an autoclave while larger fermentors are sterilized by indirect or direct steam injection.

STERILIZATION OF FERMENTATION AIR
Most fermentations are operated under high aeration and the air supplied to the fermentor must be sterilized. The number of particels and microorganisms in air varies greatly depending on the location, air movement, and previous treatment of the air
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FERMENTATION BASICS

(Date:11/26/2014)... 2014 The announced sales of three healthcare ... looking to expand in non-healthcare areas, says Kalorama Information. ... research firm says that  Siemens is the third largest ... of the IVD market , with 5.5 billion in ... composition would likely affect IVD market shares.  ...
(Date:11/26/2014)... (PRWEB) November 26, 2014 On January, ... the Baltimore and Washington D.C. science community to the ... The event, sponsored by the Protein Society, will take ... University Center, Room 312. Through discussion, presentations and ... structural importance of proteins, their role in the industry ...
(Date:11/26/2014)... November 25, 2014 Whitehouse Laboratories ... joined the Morris County (New Jersey) Chamber of ... a leading business organization in the North Jersey ... further engage some key clients in the pharmaceutical ... the geographic area. Membership enables Whitehouse Laboratories a ...
(Date:11/26/2014)... November 25, 2014 Continuing its award-winning ... earned three prestigious MarCom Awards for outstanding creative achievement ... a Platinum MarCom Award, the organization’s top honor, in ... St. Louis held in April of this year. The ... 14,000 attendees. Additionally, Nerium was awarded a Gold MarCom ...
Breaking Biology Technology:Kalorama: Sale of Three Siemens Healthcare Units Raises Questions About Firm's IVD Future 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 2
... New Analysis of the 104 ABPM Study Presented at the American Heart ... Association ... http://www.nicox.com , ... a new analysis of the data,from the 104 Ambulatory Blood Pressure Monitoring (ABPM) study ...
... "Horror In The Wind," has been banned by Allen Theaters of ... screen the locally produced comedy because it is too political. ... reverses the whole world,s sexual orientation. , ... Alamogordo, ...
... LOUIS, Nov. 11 At the Board of ... cash dividend of,$.13 per share. The dividend is ... on December 1, 2008., About Sigma-Aldrich: Sigma-Aldrich ... Our biochemical and organic chemical products and kits,are ...
Cached Biology Technology:NicOx' Naproxcinod Shows Highly Significant Reduction in Daytime Blood Pressure Versus Naproxen 2NicOx' Naproxcinod Shows Highly Significant Reduction in Daytime Blood Pressure Versus Naproxen 3Comedy 'Horror In The Wind' Banned In New Mexico 2Comedy 'Horror In The Wind' Banned In New Mexico 3
(Date:11/3/2014)... Researchers from Australia report that low birth weight and ... (OA)-related hip replacements in adulthood. Findings published in the ... & Research , indicate that low birth weight and ... arthroplasty due to OA as adults. , According ... of 25 are diagnosed with clinical OA. Symptoms of ...
(Date:11/2/2014)... November 2014)—In support of a bold quest to rid ... Gates Foundation today announced an award of US$156 million ... (MVI) in building new vaccines that will interrupt the ... "accelerating to zero" agenda. Such vaccines would ensure that ... called an "immunological bed net." , This approach ...
(Date:11/2/2014)... Nate Silver and Richard Feynman walk into a bar and ... may sound like the setup to some late-night nerd sketch, ... increasingly cumbersome problem in modern biology, namely, finding meaning in ... instance, the data comprisesreams of cancer mutations that genome-wide studies ... new and efficient ways to parse the signal from the ...
Breaking Biology News(10 mins):Preterm, low birth-weight babies may need new hips in adulthood 2New malaria vaccines to prevent infection and block transmission get a shot in the arm 2New malaria vaccines to prevent infection and block transmission get a shot in the arm 3New malaria vaccines to prevent infection and block transmission get a shot in the arm 4Mutant models 2Mutant models 3Mutant models 4
... thousands of years on tiny islands surrounding Australia have evolved ... their mainland cousins. Now, new research in The American ... driven by the need to have big-mouthed babies. Mainland ... cm) long, patrol swampy areas in search of frogs, their ...
... PORTLAND, Ore. (May 15, 2012)Twenty-six scientists have received a ... Psoriasis Foundation to study psoriasisthe most common autoimmune disease ... Americansand psoriatic arthritis, an inflammatory joint and tendon disease. ... $50,000 Discovery Grant, for a total of $400,000, for ...
... in gardens, burned for heat and energy, and converted into ... that includes corn, sorghum and sugarcane. Two new, independently produced ... that likely is a parent of Miscanthus giganteus , ... M. sinensis genome. The studies reveal how a ...
Cached Biology News:Big-mouthed babies drove the evolution of giant island snakes 2Big-mouthed babies drove the evolution of giant island snakes 3National Psoriasis Foundation awards more than $2 million for research 2Maps of Miscanthus genome offer insight into grass evolution 2Maps of Miscanthus genome offer insight into grass evolution 3
Immunogen: Extract of pigmented melanoma metastases from lymph nodes. Storage: -20 C, Avoid Freeze/Thaw Cycles...
with GlutaMAXIL-glutamine...
alpha N-catenin (C-19)...
This STAT antibody sampler pack contains 8 monoclonal ,STAT antibodies....
Biology Products: