Navigation Links
FERMENTATION BASICS

catabolized to provide energy for cell maintenance, all the enthalpy associated with the oxidation of the substrate is released as heat. If a biochemical product is formed, the heat evolved lies between the heat released during maintenance and that evolved during active growth. The amount of heat evolved is related to the stoichiometry for growth and product formation, whereas the rate of heat evolution is related to the rate of microbial activity.


2.6. Sterilization
cultures at all stages, from the preliminary culture to the fermentor. A fermentor can be sterilized either by destroying the microorganisms with some lethal agent such as heat, radiation, or a chemical, or by removing the viable microorganisms by a physical procedure such as filtration.

During fermentation the following points must be observed to ensure sterility:
sterility of the culture media
sterility of incoming and outgoing air
appropriate construction of the bioreactor for sterilization and for prevention of contamination during fermentation

STERILIZATION OF THE CULTURE MEDIA
Nutrient media as initially prepared contain a variety of different vegetative cells and spores, derived from the constituents of the culture medium, the water and the vessel. These must be eliminated by a suitable means before inoculation. A number of means are available for sterilization, but in practice heat is the most often used mechanism.

A number of factors influence the success of heat sterilization: the number and type of microorganisms present, the composition of the culture medium, the pH value, and the size of the suspended particles. Vegetative cells are rapidly eliminated at relatively low temperatures such as 60 degrees Celcius for 5-10 minutes, but for destruction
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FERMENTATION BASICS

(Date:9/3/2015)... (PRWEB) , ... September 03, 2015 , ... ... release its non-invasive hydration monitoring wearable early 2016. , The device, called ... using proprietary optical and electrical sensing technology. These sensors track trending interstitial ...
(Date:9/2/2015)... (PRWEB) , ... September 02, ... ... provider of regulatory compliance, quality systems, analytical testing, education and consulting services ... with expertise in medical device products/processes development, design control, validation, quality assurance ...
(Date:9/2/2015)... , Sep. 02, 2015 ... announced the addition of Jain PharmaBiotech,s new report ... to their offering. This report follows ... characteristic that can be objectively measured and evaluated ... processes as well as pharmacological responses to a ...
(Date:9/2/2015)... ... September 02, 2015 , ... ... botanical and plant-derived compounds. BCC Research reveals in its new report that ... this market defines the category and generates the most market demand. , ...
Breaking Biology Technology:Technology Startup Halo Wearables to Unveil Non-Invasive Hydration Monitoring Device Early 2016 2NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 2NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 3NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 4NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 5NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 6NSF International Strengthens Medical Device Consulting With Acquisition of Avarent LLC 7Updated 2015 Biomarkers Report - Latest Technologies, Markets and Companies Insights to 2024 with over 1,000 References 2Updated 2015 Biomarkers Report - Latest Technologies, Markets and Companies Insights to 2024 with over 1,000 References 3Plant-derived Compounds Poised to Assume Greater Role in Global Drug Markets, According to BCC Research 2Plant-derived Compounds Poised to Assume Greater Role in Global Drug Markets, According to BCC Research 3
... BARBARA, Calif. , May 21 Abviva, Inc. (Pink ... of breast cancer related applications of Mammastatin, today provided further details ...   , , , ... Company previously announced that the Japanese Patent Office issued two patents ...
... Waters® Corporation (NYSE: WAT ) and the ... a groundbreaking partnership to establish the first facility in the ... the state-of-the-art methods of analysis that will help them meet ... International Food Safety Training Laboratory (IFSTL) will be operated by ...
... KYOTO, Japan and MARCY L,ETOILE, France ... announce today that they have entered into a partnership ... in microbiology laboratories. bioMerieux will work with Shimadzu and ... mass spectrometry technology for optimized integration into the microbiology ...
Cached Biology Technology:Abviva Details Patent Information for its Breast Cancer Risk Assessment Technology 2Abviva Details Patent Information for its Breast Cancer Risk Assessment Technology 3Groundbreaking International Training Lab to Strengthen Food Safety in U.S. and Globally 2Groundbreaking International Training Lab to Strengthen Food Safety in U.S. and Globally 3Groundbreaking International Training Lab to Strengthen Food Safety in U.S. and Globally 4Shimadzu and bioMerieux Enter into Partnership for Mass Spectrometry Applications in Microbiology 2Shimadzu and bioMerieux Enter into Partnership for Mass Spectrometry Applications in Microbiology 3Shimadzu and bioMerieux Enter into Partnership for Mass Spectrometry Applications in Microbiology 4
(Date:8/5/2015)... Calif. , Aug. 5, 2015 The ... continuous growth in applications, penetration into newer sectors, and ... year. The global biosensors space has seen the entry ... the market so far. (Photo - ... & Sullivan, Analysis of the Global Biosensors Market ...
(Date:8/4/2015)... AMRI ) today reported financial and operating results for the ... Second quarter contract revenue of $ 85.2 ... contract margins of 26 % , Second ... EPS from royalties in the current quarter , ... very pleased to present another strong financial quarter, with all our ...
(Date:7/31/2015)... , Kina, 31. juli 2015 Den 10. ... afholdt af BGI fra den 22. - 25. oktober i ... Konferencen fejrer sin 10-års fødselsdag i år. Siden starten ... indflydelsesrige årlige møder på ,omik,-feltet, og er en af ... ICG-10 fokuserer på nylige gennembrud og fremskridt ...
Breaking Biology News(10 mins):Innovative Biosensors Incite Use in Non-Traditional Applications 2Innovative Biosensors Incite Use in Non-Traditional Applications 3Innovative Biosensors Incite Use in Non-Traditional Applications 4AMRI Announces Second Quarter 2015 Results 2AMRI Announces Second Quarter 2015 Results 3AMRI Announces Second Quarter 2015 Results 4AMRI Announces Second Quarter 2015 Results 5AMRI Announces Second Quarter 2015 Results 6AMRI Announces Second Quarter 2015 Results 7AMRI Announces Second Quarter 2015 Results 8AMRI Announces Second Quarter 2015 Results 9AMRI Announces Second Quarter 2015 Results 10AMRI Announces Second Quarter 2015 Results 11AMRI Announces Second Quarter 2015 Results 12AMRI Announces Second Quarter 2015 Results 13AMRI Announces Second Quarter 2015 Results 14AMRI Announces Second Quarter 2015 Results 15AMRI Announces Second Quarter 2015 Results 16AMRI Announces Second Quarter 2015 Results 17AMRI Announces Second Quarter 2015 Results 18AMRI Announces Second Quarter 2015 Results 19AMRI Announces Second Quarter 2015 Results 20AMRI Announces Second Quarter 2015 Results 21AMRI Announces Second Quarter 2015 Results 22AMRI Announces Second Quarter 2015 Results 23AMRI Announces Second Quarter 2015 Results 24Den 10. internationale konference om Genomics (ICG-10) åbner i oktober 2
... the obesity epidemic is that it has resulted in ... also tend to have higher birth weights and more ... and chronic diseases later in life. However, the nutritional ... may influence child obesity remain uncertain. A recent publication ...
... have their way, biological research may soon be transformed by ... injected into individual cells without harm to the host. Welcome ... and medicine in which light-based devices lasers and light-emitting ... study and influence of living cells. The team ...
... ramp up your romance this year by sharing a candlelight dinner, ... perfume, but will that help you find your perfect mate? For ... love potion to know whether he,s "Mr. Right." Unlike humans, ... a mate. Scientists have found that sex pheromones play an important ...
Cached Biology News:Preventing obesity transmission during pregnancy 2Light-emitting bioprobe fits in a single cell 2Light-emitting bioprobe fits in a single cell 3Light-emitting bioprobe fits in a single cell 4Finding 'Mr. Right,' how insects sniff out the perfect mate 2Finding 'Mr. Right,' how insects sniff out the perfect mate 3
High-throughput DNA sequencing of any library. Just give us your transformation mixture, and we'll do the rest. ABI 3730xl's yield the longest read lengths available. LIMS tracking utilizing Geospiza...
... pass sequencing of plasmids and PCR ... our high performance sequencing pipeline which ... per day. The key benefits we ... are rapid turnaround, high sequencing pass ...
... high quality whole genome sequencing service. Our ... allow even the smallest of laboratories to ... features which set our whole genome sequencing ... strategies, proprietary assembly software, rapid project turnaround ...
Shotgun library construction and high-throughput DNA sequencing of microbial genomes, BACs, adenoviruses, phosmids and cosmids. LIMS tracking utilizing Geospiza's Finch system....
Biology Products: