Navigation Links
Escherichia coli K12

e for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 x g, 2-4 C). Resuspend pellet in 5 ml ice-cold water. Keep the cells cold during the entire procedure. 3. Wash twice with the original culture volume of ice-cold water. Centrifuge as above. Resuspend pellet by swirling in remaining liquid. 4a. If using the cells immediately, place suspension in a prechilled tube and centrifuge (10 minutes, 5,000 x g, 2-4 C). Resuspend the cells in ice-cold water to a final concentration of approximately 2 x 1011 cells/ml. Aliquote 40-300 l cells into prechilled centrifuge tubes. 4b. If freezing the cells for later use, add 40 ml of ice-cold 10% glycerol, mix and centrifuge for 10 minutes, at 5,000 x g and 2-4 C. Resuspend the cells in ice-cold 10% glycerol to a final concentration of approximately, 2 x 1011 cells/ml. Aliquote 40-300 l cells into prechilled centrifuge tubes. quick freeze on dry ice and store at 80 C.

Electroporation of cells:

  1. Add 1 l DNA (10 pg in water) to tubes containing 40 l electrocompetent cells. Homogenize by gently mixing with
    pipette several times.
  2. Transfer mixture to a prechilled cuvette. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O V
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Escherichia coli C600
2. Escherichia coli DH10B
3. Escherichia coli DH5
4. Escherichia coli C600
5. Escherichia coli K12
6. Escherichia coli DH10B
7. Escherichia coli DH5
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Escherichia coli

(Date:9/19/2014)... 2014 Biotech companies make announcements ... drug systems and advanced Laboratory Instrumentation. Companies in focus ... BDX ), Thermo Fisher Scientific Inc. (NYSE: ... RGDO ), Sangamo Biosciences Inc. (NASDAQ: ... Pressure BioSciences, Inc. (OTCQB: PBIO) ("PBI" or the "Company") ...
(Date:9/19/2014)... Barnhardt Manufacturing Company has ... Organic Cotton . The USDA Certified Biobased Product ... ingredients meets or exceeds levels set by USDA. Biobased ... or in significant part of agricultural, forestry, or marine ... cotton to be immediately identified as a USDA certified ...
(Date:9/19/2014)... The new , Healthcare and Life Sciences Review ... PharmaBoardroom.com for free download , looks beyond ... economy in 2009, and the austerity measures that followed, to ... times of trouble. "Despite the crisis, ... and the general conditions to invest are favorable to the ...
(Date:9/18/2014)... -- DeepResearchReports.com adds "2014 Deep Research Report on ... Deep Research Report on Global and China Sodium ... Photo - http://photos.prnewswire.com/prnh/20140918/147058 ... China Acetic Acid Industry" is a professional and ... and Global Acetic Acid Market. The report ...
Breaking Biology Technology:Medical Instrumentation Automation Advances for Biological Sample Preparation - Company Expects Q4 Revenue Impact from Newest Instrument Systems 2Medical Instrumentation Automation Advances for Biological Sample Preparation - Company Expects Q4 Revenue Impact from Newest Instrument Systems 3Medical Instrumentation Automation Advances for Biological Sample Preparation - Company Expects Q4 Revenue Impact from Newest Instrument Systems 4Medical Instrumentation Automation Advances for Biological Sample Preparation - Company Expects Q4 Revenue Impact from Newest Instrument Systems 5Medical Instrumentation Automation Advances for Biological Sample Preparation - Company Expects Q4 Revenue Impact from Newest Instrument Systems 6Medical Instrumentation Automation Advances for Biological Sample Preparation - Company Expects Q4 Revenue Impact from Newest Instrument Systems 7Barnhardt Manufacturing Company Earns USDA Certified Biobased Product Certification and Label for Organic Cotton 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 3Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 2Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 3Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 4
... , , , ... of medical research management software for the Life Sciences industry, today ... Internet application of its Protocols for Animal Studies which is focused ... Use Committees (IACUCs). , , Developed to address ...
... , , CARLSBAD, Calif., ... water and microbial nucleic acid purification, announces the launch of an ... total RNA from a variety of filtered water samples. PowerWater(R) ... the developing fields of water safety testing, microbial community analysis and ...
... , , , ... Octapharma AG recently submitted its Biological License Application ... liquid) to the U.S. Food and Drug Administration ... immune globulin therapy portfolio by early 2010. The application was submitted ...
Cached Biology Technology:TOPAZ Technologies Protocols for Animal Studies Software Drives Customer Conversions 2MO BIO Laboratories, Inc. Launches PowerWater(R) RNA Isolation Kit 2Octapharma Aims to Expand U.S. Immune Globulin Therapy Portfolio 2Octapharma Aims to Expand U.S. Immune Globulin Therapy Portfolio 3
(Date:9/21/2014)... to what was previously thought, newborn immune T cells ... to bacteria, according to a new study led by ... differently to that of adults, babies may still be ... study published in the journal Nature Medicine . ... different types of immune cells, including neutrophils which play ...
(Date:9/21/2014)... has developed a protein therapy that disrupts the process ... tumor sites, travel through the blood stream and start ... process, known as metastasis, can cause cancer to spread ... who succumb to cancer fall prey to metastatic forms ... of bioengineering who describes a new therapeutic approach in ...
(Date:9/19/2014)... from several Harvard University labs in collaboration with ... researchers with the intellectual raw materials needed to ... flexible materials. , With the advent of low-cost ... manufacturing technology, soft robotics is emerging as an ... rigid robot design, but working with pliable materials, ...
Breaking Biology News(10 mins):Immune system of newborn babies is stronger than previously thought 2Stanford researchers create 'evolved' protein that may stop cancer from spreading 2Stanford researchers create 'evolved' protein that may stop cancer from spreading 3Stanford researchers create 'evolved' protein that may stop cancer from spreading 4Soft robotics 'toolkit' features everything a robot-maker needs 2
... drug to fight bird flu that will not suffer from ... begin clinical trials within the next three years, thanks to ... University of Bath (UK) and Dr Jennifer McKimm-Breschkin from CSIRO ... Council (MRC) to develop a new class of inhibitor they ...
... air pollution don't take atmospheric chlorine into account, but ... more of daily ozone production in local air, research ... nearly nonstop in the Irvine area over a two-month ... five parts per trillion or less, but occasionally reached ...
... early days of X-ray crystallography obtaining a three-dimensional ... bolts and years of tedious calculations by hand. ... to sophisticated software and in particular a package ... Hamburg Outstation of the European Molecular Biology Laboratory ...
Cached Biology News:New bird flu drug promises to beat the problem of resistance 2New bird flu drug promises to beat the problem of resistance 3UCI scientists find chlorine may contribute to ozone formation 2Mapping the protein world 2
... Ascent is the first nephelometer ... microplates. Nepheloskan measures light scattering ... for particle quantification. This makes ... in drug discovery, immunoprecipitation, and ...
Anti-VDAC (Voltage-Dependent Anion Channel) AVOID FREEZE/THAW CYCLES. Recognizes the porin 31HL protein. Corresponds to mAb 1 in the literature....
6 x Blend of rTth DNA Polymerase and Tli to provide optimized amounts of both 5-3 and 3-5 proofreading activity...
... Rotarods are used to assess the effect of ... on mice and rats using one unit in ... the animals are placed on textured drums to ... individual sensing platforms below, its test results are ...
Biology Products: