Navigation Links
Epitope-Tagging Vectors for Functional Analysis in Yeast


Epitope-Tagging Vectors for Functional Analysis in Yeast

Regulation of gene expression in yeast

Quinn Lu Tanya Hosfield
Stratagene

Stratagene has developed a series of epitope-tagging vectors to express and functionally analyze eukaryotic genes in the budding yeast Saccharomyces cerevisiae. Each of these pESC vectors contains one of four different yeast selective markers in the same vector backbone, which allows expression and epitope-tagging analysis of two different genes in a single yeast cell.

Characterizing the structure and function of every eukaryotic gene is a necessary task in the post genome era. Results of the yeast genome sequencing project indicate that S. cerevisiae shows homologous sequences for one-third of the human disease genes discovered to date.1 Hence, the yeast makes an attractive model for studying these genes.

We constructed a series of epitope-tagging vectors to facilitate the in vivo study of eukaryotic genes in S. cerevisiae. With these vectors, any cloned gene can be introduced into yeast under the control of a repressible promoter, two different genes can be coexpressed in yeast, protein-protein interactions can be confirmed by immunoprecipitation analysis, and protein localization can be studied. Together with the use of other vectors, the pESC vectors can be used to characterize functional domains of an essential gene and genetically screen for suppressors and inhibitors of certain types of genes in yeast.

pESC Vectors

Figure 1

The pESC vectors (Figure 1) are specifically designed to express high levels of eukaryotic proteins in S. cerevisiae. They are derived from the pRS400 series vectors.2 Each pESC vector contains the following seque
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
4. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. Versatile Reporter Vectors for Monitoring Viral Transduction
7. High-Titer Retroviral Vectors for Gene Delivery
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Mammalian Expression Vectors with Interchangeable Selectable Markers
10. Delivering siRNA Using Adenoviral Vectors
11. Drive Long-Term Silencing with CMV Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Epitope Tagging Vectors for Functional Analysis Yeast

(Date:7/5/2015)... ... July 06, 2015 , ... For many years, metal-on-quartz filters ... a high-end machine such as a Varian Cary 4000-7000, a Perkin Elmer 800-1100 or ... design that even NIST used them for their SRM-2031 series going back for many ...
(Date:7/3/2015)... (PRWEB) , ... July 03, 2015 , ... ... highly-regarded by Fortune 500 companies and professional service industries alike-- typically known for ... make clients laugh, honor a good cause and break from their day jobs. ...
(Date:7/3/2015)... Forskere kan søke ... Science", som finner sted i ... på  http://www.openinnovationinscience.at , og søknadsperioden varer frem ... to største utfordringene innen helsevitenskapen er mangelen ... og kompleksiteten av nåværende forskningsresultater, i følge ...
(Date:7/2/2015)... ... July 03, 2015 , ... Cuvettes have been used in countless labs for decades. ... the cuvette they needed. As expected, this was a daunting and tiring process looking ... because they were not able to navigate the complex catalog structure. , Now thanks to ...
Breaking Biology Technology:FireflySci Simplifies the Calibration of UV Spectrophotometers with FUV Photometric Accuracy Standards 2FireflySci Simplifies the Calibration of UV Spectrophotometers with FUV Photometric Accuracy Standards 3The Alexander Group Declares Their Independence with a Bit of Levity 2Lansering av verdens første Open Innovation-program for utdanning av forskere 2Lansering av verdens første Open Innovation-program for utdanning av forskere 3New Cuvette E-Commerce Website Launched by FireflySci 2
... devised by researchers from the National Institute of Standards ... flame retardants used in the polyurethane foam of upholstered ... perhaps by as much as 1,130 percent. The ... ,* suggest that significant fire-safety advantages can be gained ...
... Susan G. Komen for the Cure ® Los ... cancer advocacy organization, is proud to announce a partnership with ... advocate, Kelly Price .  Susan G. Komen for the ... and education. The Los Angeles County Affiliate ...
... 3, 2011 HumanTouch, LLC announced that it ... contract by the U.S. General Services Administration. 8(a) ... contract engineered to provide cutting-edge technology solutions from ... designation is designed to promote small business utilization ...
Cached Biology Technology:Novel coatings show great promise as flame retardants in polyurethane foam 2Kelly Price Joins Los Angeles County Affiliate of Susan G. Komen for the Cure in the Fight Against Breast Cancer 2Kelly Price Joins Los Angeles County Affiliate of Susan G. Komen for the Cure in the Fight Against Breast Cancer 3Kelly Price Joins Los Angeles County Affiliate of Susan G. Komen for the Cure in the Fight Against Breast Cancer 4HumanTouch Awarded 8(a) STARS II GWAC Contract 2
(Date:6/18/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company") ... market announces that its Wocket® smart wallet will be the ... scheduled to air on WABC Radio (am770) in ... The broadcast air- time for the extensive one ... Inc.,s CEO Gino Pereira will be discussing its ...
(Date:6/17/2015)... June 17, 2015  Crossmatch™, a leading provider ... its U.are.U ® 4500 fingerprint readers have ... Eateries, Inc., a San Jose, CA ... increase security, improve accountability and reduce fraud at ... non-repudiable identity confirmation for employee sign in and ...
(Date:6/16/2015)... 16, 2015 Fingerprint Cards (FPC) raises ... it will amount to at least 340 MSEK to that ... rapid increase in market growth and orders received the revenue ... that revenue for 2015 will exceed 1,500 MSEK to an ... 2,200 MSEK. Due to receipt of orders of ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 2Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 3
... factors that signal whether an athlete will experience concussive ... risk factors for prolonged concussion symptoms by examining a ... concussion studies were limited in scope, focusing only on ... to male and female athletes from a number of ...
... intensive care units have difficulty finding private, quiet places ... new study from the Frances Payne Bolton School of ... of privacyif it causes mothers to miss feedings or ... greater health risk for low-weight premature newborns, because milk ...
... Scientists at Singapore Eye Research Institute and A*STAR,s Genome ... central corneal thickness that may cause potentially blinding eye ... the progressive thinning of the cornea, which may eventually ... authors jointly led a multi-centre study involving 55 hospitals ...
Cached Biology News:Privacy a problem for mothers of newborns in neonatal intensive care units, CWRU study finds 2First in the world - Singapore scientists discover genes responsible for cornea blindness 2First in the world - Singapore scientists discover genes responsible for cornea blindness 3
Acrylamide, 500 g. Assay: > 99.9%, acrylic acid: < 0.001%. Conductivity (35%): < 2 umhos/cm.Risks: 45, 46, 48, 23, 24, 25 ; Safety Precautions: 53, 44. Category: Nucleotides & Enzymes & Biochemicals...
HRP Monoclonal-Goat Diluent - 5X Concentrate, 25 mL...
...
... the standard water based mounting medium for ... permanent preservation of peroxidase and alkaline phosphatase ... use and exhibits excellent optical characteristics. Crystal ... medium that permits the long-term storage of ...
Biology Products: