Navigation Links
Epitope-Tagging Vectors for Functional Analysis in Yeast


Epitope-Tagging Vectors for Functional Analysis in Yeast

Regulation of gene expression in yeast

Quinn Lu Tanya Hosfield
Stratagene

Stratagene has developed a series of epitope-tagging vectors to express and functionally analyze eukaryotic genes in the budding yeast Saccharomyces cerevisiae. Each of these pESC vectors contains one of four different yeast selective markers in the same vector backbone, which allows expression and epitope-tagging analysis of two different genes in a single yeast cell.

Characterizing the structure and function of every eukaryotic gene is a necessary task in the post genome era. Results of the yeast genome sequencing project indicate that S. cerevisiae shows homologous sequences for one-third of the human disease genes discovered to date.1 Hence, the yeast makes an attractive model for studying these genes.

We constructed a series of epitope-tagging vectors to facilitate the in vivo study of eukaryotic genes in S. cerevisiae. With these vectors, any cloned gene can be introduced into yeast under the control of a repressible promoter, two different genes can be coexpressed in yeast, protein-protein interactions can be confirmed by immunoprecipitation analysis, and protein localization can be studied. Together with the use of other vectors, the pESC vectors can be used to characterize functional domains of an essential gene and genetically screen for suppressors and inhibitors of certain types of genes in yeast.

pESC Vectors

Figure 1

The pESC vectors (Figure 1) are specifically designed to express high levels of eukaryotic proteins in S. cerevisiae. They are derived from the pRS400 series vectors.2 Each pESC vector contains the following seque
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
4. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
5. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
6. Versatile Reporter Vectors for Monitoring Viral Transduction
7. High-Titer Retroviral Vectors for Gene Delivery
8. Improved Vectors for PathDetect Trans-Reporting Systems
9. Mammalian Expression Vectors with Interchangeable Selectable Markers
10. Delivering siRNA Using Adenoviral Vectors
11. Drive Long-Term Silencing with CMV Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Epitope Tagging Vectors for Functional Analysis Yeast

(Date:1/15/2014)... "The interface is the device," Nobel laureate Herbert ... to be found at the junctures where layers ... of nanotechnology, the interfaces between layers of metal ... such high-tech favorites as spintronics, high-temperature superconductors, ferroelectrics ...
(Date:1/15/2014)... 15, 2014 Cynvenio, a cancer diagnostics ... the genomic analysis of tumor cells in the bloodstream, ... web-based cancer decision support application powered by CollabRx, Inc. ... ClearID™ genomic test, Therapy Finder will provide breast cancer ...
(Date:1/15/2014)... More than 5 million Americans are currently ... seniors will die with Alzheimer’s or another dementia, according ... shocked many Americans into looking for ways to improve ... age-related cognitive disorders. Jonathan Weisman, president of Biohack Pure, ...
(Date:1/15/2014)... Freeslate, Inc ., the leading ... Lupin Limited, one of India’s top five pharmaceutical companies, ... System for high throughput solid form screening. ... a wide range of quality, affordable generic and branded ...
Breaking Biology Technology:A deeper look at interfaces 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3
... OMAHA, Neb., April 1, 2011 Transgenomic, ... scientists have completed studies employing the company,s high-sensitivity ... COLD-PCR and BLOCker™-Sequencing.  Results from these studies will ... Research (AACR) meeting in Orlando, Florida, April 2-6, ...
... National Institute of Standards and Technology (NIST) have developed a ... probes used to study nanoscale structures in situ and as ... improve the accuracy of the most precise and delicate nanoscale ... trying to measure the contours of a surface with a ...
... /PRNewswire-Asia-FirstCall/ -- China-Biotics, Inc. (Nasdaq: CHBT ) ... distributor of probiotics products in China, today announced that ... its newly signed U.S. customer. The U.S. ... and formally entered into its agreement with China-Biotics on ...
Cached Biology Technology:Transgenomic, Inc. Presentations to Describe High-Sensitivity Detection of Cancer Biomarkers at the 2011 American Association for Cancer Research Meeting 2Transgenomic, Inc. Presentations to Describe High-Sensitivity Detection of Cancer Biomarkers at the 2011 American Association for Cancer Research Meeting 3Transgenomic, Inc. Presentations to Describe High-Sensitivity Detection of Cancer Biomarkers at the 2011 American Association for Cancer Research Meeting 4Getting the point: Real-time monitoring of atomic-microscope probes adjusts for wear 2China-Biotics Receives First U.S. Sales Order 2China-Biotics Receives First U.S. Sales Order 3
(Date:4/23/2014)... in French and German . ... humans and other mammals, the difference between sexes depends on one ... only in males, where the two sexual chromosomes are X and ... ultimately responsible for all the morphological and physiological differences between males ... A very long time ago, the X and Y were identical, ...
(Date:4/23/2014)... Scarborough research shows that male black widow spiders prefer ... rare example of mate preference by male spiders. ... MacLeod and Maydianne Andrade, a professor in UTSC,s Department ... and the wild that males overwhelmingly chose to mate ... widows can tell whether a potential mate is well-fed ...
(Date:4/23/2014)... (4/23/14) -- Researchers have found evidence of an ... that increase aggressive behavior in children, especially in ... all children exposed to prenatal smoking will have ... not," said Brian Boutwell, Assistant Professor at Sam ... senior author on the study. "One possible ...
Breaking Biology News(10 mins):Male or female? 2Male or female? 3Picky male black widow spiders prefer well-fed virgins 2Genetics risk, prenatal smoking may predict behavioral problems 2
... Clonorchis sinensis affects more than 35 million people ... by this parasite causes clonorchiasis. Repeated or chronic infection can ... bile ducts, including the frequently fatal bile duct cancer - ... C. sinensis , published in BioMed Central,s open access journal ...
... researchers found a way of identifying ideal drug combinations ... occurring. The findings, published in Nature Chemical ... development of new drug combinations to combat severe diseases ... stroke and Alzheimer,s are worsened by inflammation, which is ...
... genetically engineered clotting factor that controlled hemophilia in an ... hemophilia and a broad range of other bleeding problems. ... Xa (FXa), a protein active in blood clotting, and ... bleeding in mouse models of hemophilia. "Our designed variant ...
Cached Biology News:Liver parasite lacks key genes for fatty acid synthesis: Genome sequencing of Clonorchis sinensis 2Manchester's 'first step' to perfect drug combinations 2Bioengineered protein shows preliminary promise as new therapy for hemophilia 2
... a software package that provides crystallographers ... custom buffer creation, set-up of experimental ... trials. The software helps crystallographers through ... protein crystallography: Design ...
The PROTEAN II slab gel casting stand is a component of the PROTEAN II XL and xi casting apparatus. It is supplied with gaskets....
Mouse monoclonal [H4B4] to LAMP2 - Lysosome Marker (Phycoerythrin) ( Abpromise for all tested applications). entrezGeneID: 3920 SwissProtID: P13473...
... imaging software for the microscopy market ... cameras, components and peripherals with powerful ... Its intuitive interface simplifies workflow and ... providing powerful features such as image ...
Biology Products: