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Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH

act of the cannulas with the plates is required when transferring sub-microliter volumes to dry plates, potentially resulting in variable %CV's (unpublished data). For the above results, we used a shorter, finer 384/1536 Cannula Array designed for accessing 384 well plates or 1536 plates (by dispensing into four quadrants). This type of a cannula array can fully access standard microplates, or half height deep well plates. The main benefit of the smaller cannulas is that uniform droplets can form on the ends of the finer cannulas, allowing the dispense of the droplet onto the plate without contacting the plate.

The success of dry dispensing depends on assuring the Cannula Array and destination plates are parallel. Therefore, it is necessary to level any position in which sub-microliter dry dispensing is attempted. The deck of the Sciclone ALH is designed to be adjustable at each corner. The individual plate locators must also be level. It is also necessary to determine a precise dispense height for any non-contact dispense operation.

Physical factors are an important determinant of overall dispensing performance. Such factors include microplate composition, microplate surface treatments, solvent concentrations and compositions, solute (compound) characteristics and solute concentration. The current study represents 2.8g/L tartrazine dissolved with DMSO and dispensed into untreated, flat-bottomed, polystyrene plates only. Under these conditions we demonstrated that it is feasible to dispense sub microliter volumes to dry plates with very good precision.


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