Navigation Links
Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH

ettor. Any air bubbles are removed, and the plate is read for absorbance. The average absorbance reading for these manual standards is used in a single point calibration routine.

Sciclone Protocols

Here a single aspiration step is performed, followed by priming dispenses, then incremental dispenses into dry plates as follows:

Data Acquisition and Processing

Absorbance data is acquired on a Wallac Victor2 reader using the same read protocol as for the calibration plate. The raw results are exported to ExcelTM and %CV is calculated. The volume dispensed is calculated by comparison of signal to a manually prepared calibration standard.

Results

Testing has been done for three and five incremental plates using the method described above. For each run, the volume dispensed and %CV for each plate is calculated and summarized (Table 1). The results for the two runs are represented graphically (Figure 3).

Discussion

In this preliminary work we have found that the Sciclone ALH equipped with a Low Volume Head is capable of delivering 0.5μL of liquid into dry microplates with a precision of less than 6%CV. This is performed with no direct contact between the cannulas and the plate. In the above results, a "first plate" effect is observed, in which the first plate in a series has a higher volume. We believe this is due to residual tartrazine solution of the external surfaces of the cannulas that is removed as the first plate is dispensed.

Initial dry plate work was carried out using a 384 Cannula Array. The benefits of this array are that it can access both deep well plates and standard microplates. However, the larger cannulas do not permit formation of well formed droplets. Without such droplets, physical cont
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting FlashPlates on the Wallac MicroBeta
3. Direct Counting of Millipore MultiScreen Filtration Plates.
4. Sciclone ALH Bulk Dispense Precision Testing
5. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
6. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
7. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
8. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
9. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
10. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Dispensing Dry Plates with the Sciclone ALH

(Date:5/28/2015)... NORTH BRUNSWICK, N.J. , May 28, 2015 ... that PDS0101, a cancer immunotherapy treatment targeting HPV-16, ... patients.  Currently, pre-cervical cancer is ... could offer a simple and effective non-surgical alternative. ... body,s defense mechanisms (T-cells) to recognize, target, and ...
(Date:5/28/2015)... Md. , May 28, 2015  GenVec, Inc. ... and CEO, Douglas J. Swirsky , will present ... Conference at 11 a.m. EDT on Thursday, June 4, ... of Mr. Swirsky,s presentation will be available at the ... be available on GenVec,s website.  To access, visit ...
(Date:5/28/2015)... Sunnyvale, Californiaa (PRWEB) May 28, 2015 ... on “ The Changing Landscape of EMR/EHR Clinical Data ... Annual Meeting taking place in Washington, DC on June ... the challenges of integrating EMR/EHR systems with EDC, and ... the opportunities brought by eSource and electronic health records/electronic ...
(Date:5/28/2015)... American Process Inc. (Atlanta, GA) and ... signed a joint agreement to improve ... nanocellulose, a rapidly emerging high performance, bioderived nanomaterial. ... limited number of feedstock materials available. Traditionally used ... parts for load bearing applications. , The ...
Breaking Biology Technology:PDS Biotechnology reports positive Phase I clinical trial results in proprietary HPV-Cancer Immunotherapy 2GenVec To Present At The Jefferies Healthcare Conference 2Clinovo Presents at the DIA 2015 51st Annual Meeting in Washington, DC on June 14th-18th 2American Process Inc. Announces Partnership with Oak Ridge National Laboratory to Make 3D Printing Plastic Resins Stronger and More Sustainable 2American Process Inc. Announces Partnership with Oak Ridge National Laboratory to Make 3D Printing Plastic Resins Stronger and More Sustainable 3
... SOUTH SAN FRANCISCO, Calif., May 5 VaxGen,Inc. ... it has completed the sale of all assets ... anthrax vaccine product candidate and,related technology to Emergent ... BioSolutions paid VaxGen $2 million upon execution of,the ...
... May 2 Biopure Corporation,(Nasdaq: BPUR ) ... Kingdom,s,Medicines and Healthcare Products Regulatory Agency (MHRA) is ... The purpose of the meeting is to clarify ... April 2008 from the United Kingdom,s Commission,on Human ...
... Roche is pleased to announce that,Grace Ju, Ph.D., research ... an "Outstanding Woman of Science" by the New Jersey,Association ... Thank You,Research Gala held May 1 at the Bridgewater ... at this year,s event, which is also,marking the 20th ...
Cached Biology Technology:VaxGen Sells Anthrax Vaccine Program and Related Assets and Technology to Emergent BioSolutions, Inc. 2Biopure Announces Date to Meet with UK's Medicines and Healthcare Products Agency 2Biopure Announces Date to Meet with UK's Medicines and Healthcare Products Agency 3Roche Research Director Grace Ju Hailed as NJABR 'Outstanding Woman of Science' 2Roche Research Director Grace Ju Hailed as NJABR 'Outstanding Woman of Science' 3
(Date:5/18/2015)... Fingerprint Cards (FPC) has received an ... and FPC1035 from one if its module partners. Deliveries are ... 2015 and the sensors will be used by smartphone manufacturers ... 2015 hereby amount to 740 MSEK to date. In addition ... and a number of smaller orders not separately communicated amounting ...
(Date:5/14/2015)... 14, 2015  Verificient Technologies, Inc., a ... online remote proctoring, announced a new alliance ... and creator of the Canvas Learning Management ... companies will benefit from the seamless integration ... As a fully integrated multifactor biometrics behavioral ...
(Date:5/11/2015)... May 11, 2015  Through a well-rounded UAS delegation representing ... had a strong showing at AUVSI,s Unmanned 2015 conference last ... Ohio,s UAS industry met with over ... all points along the UAS ecosystem. "Our ... Vice President for Aerospace Rich Knoll . "If you ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Verificient and Instructure Announce Alliance Partnership to Provide Online Proctoring for K-12 and Higher Education Institutions 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2
... hydrogen gas the ultimate fuel for stars is ... Robert Braun has found.,This is the first accurate measurement of ... after the Big Bang the Universe,s matter was almost entirely ... and generated galaxies, stars and planets and the process ...
... a fjord in Canada scientists have found a landscape similar ... a frozen and sulphurous environment, where sulphur associated with Arctic ... for traces of life on Europa. It is ... and sulphur come together, supposedly like on Europa, Jupiter,s moon. ...
... LA JOLLA, CA----A new method for rapidly solving the ... as integral membrane proteins, may speed drug discovery by ... to a paper published May 20 in Nature ... the Salk Institute for Biological Studies, provides a shortcut ...
Cached Biology News:There's more star-stuff out there but it's not dark matter 2Arctic bacteria help in the search to find life on moon Europa 2Speeding up drug discovery with rapid 3-D mapping of proteins 2Speeding up drug discovery with rapid 3-D mapping of proteins 3
Matrix for protein MALDI-MS...
Originally developed for the culture of Drosophila; suitable for culture of other dipteran cell lines. Powder, Insect cell culture tested...
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. Mfr Desig: ...
Collected from calves that are 10 days old or less. Sterile, cell culture tested, US Origin...
Biology Products: