Navigation Links
Dispensing 0.5μL to "Dry" 384 Plates with the Sciclone ALH

Mike Benway, Larry Simonson and John Ung
Caliper Life Sciences Inc, Applications Laboratory, Hopkinton, Massachusetts, USA

Introduction

"Assay-Ready" plates prepared from compound or library stock plates in real time greatly accelerates the High Throughput Screening (HTS) process. The ability to transfer and dilute 0.5μL volumes is a prerequisite for this function. Here we present preliminary data on 0.5μL dry dispensing using the Caliper Life Sciences Sciclone Advanced Liquid Handler (ALH) Workstation, configured with a Low Volume Head and 384/1536 Fixed-Cannula Array.

Materials and Methods

Instrumentation

All testing was performed using a Sciclone ALH with a Low Volume Dispensing Head, and a Bulk Reagent Dispenser (Caliper Life Sciences). Absorbance was measured at 450nm using a Wallac Victor2 1420 Multilabel Counter (PerkinElmer).

Reagents

Tartrazine was obtained from Sigma Chemical Company, Catalogue number T-0388. A stock dispensing solution was prepared by dissolving 2.8g of tartrazine into 1L of DMSO. The stock solution was prepared for dispensing volumes of 0.5μL. Deionized water (DI) was used as a diluent after dispensing into the dry plate.

Accuracy Determination

Dispense accuracy is determined before measuring dry dispense precision. A calibration is performed to determine the volume dispensed. For the calibration, 50μL of deionized water is pipetted in to a 384 Well Flat Bottom, Untreated Polystyrene Assay Plates (COSTAR, Catalogue # 3702). Next, 0.5μL of the tartrazine solution is manually injected into each of 20 wells, successively, from a filled 10μL Hamilton micro syringe. The wells are manually mixed by multiple aspirate- dispense cycles using a 50μL hand pip
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Counting Colonies or Plaques Directly from Images of Agar Plates
2. Counting FlashPlates on the Wallac MicroBeta
3. Direct Counting of Millipore MultiScreen Filtration Plates.
4. Sciclone ALH Bulk Dispense Precision Testing
5. 96 Well Pipetting Precision Testing Using the Sciclone ALH with a 384 Fixed-Cannula Array
6. Sciclone ALH 3000 Liquid Handler: Functional Characterization of the High Volume Head - Part 1: Plate-to-Plate Precision Testing
7. Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
8. Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option
9. 384 Well Pipetting Precision and Accuracy Testing Using the Sciclone Advanced Liquid Handler
10. Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Dispensing Dry Plates with the Sciclone ALH

(Date:9/19/2014)... September 19, 2014 BioTech News ... patents and results of studies and trials.  Companies in ... Company (NYSE: LLY ), Gilead Sciences Inc. ... CELG ) and Arena Pharmaceuticals Inc. (NASDAQ: ARNA) ... developing prodrug therapeutics for the treatment of cancer, announced ...
(Date:9/19/2014)... -- Dublin ... the addition of the  "Micro Market Monitor : ...      (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , , ,The ... segments in the chromatography market. The market was ... to reach $2.0 billion by 2019, at a ...
(Date:9/18/2014)... USA (PRWEB) September 18, 2014 Bipartisan ... of a bill designed to stimulate development and commercialization ... is being praised by leaders of SPIE, the ... the Revitalizing American Manufacturing and Innovation (RAMI) Act, now ... bill’s authors have said they are optimistic that it ...
(Date:9/18/2014)... 18, 2014 /PRNewswire-iReach/ -- DeepResearchReports.com adds "2014 Deep ... Industry" and "2014 Deep Research Report on Global ... research database. Photo - http://photos.prnewswire.com/prnh/20140918/147058 ... on Global and China Acetic Acid Industry" is ... China and Global Acetic Acid ...
Breaking Biology Technology:Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 2Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 3Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 4Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 5Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 6Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 7Micro Market Monitor : Global Pre-packed Chromatography Columns 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 3Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 2Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 3Acetic Acid Market & Sodium Stannate Industry (China, Global) Research Report 4
... In conjunction with,its third quarter 2007 earnings release (expected to ... CVD ) is pleased to invite you to,listen to ... the Internet on Thursday, October 25 at 9:00 a.m. ET. ... and Slide Presentation When: October 25, 2007, ...
... Pharmasset, Inc.,(Nasdaq: VRUS ) has entered into ... Subject to certain terms and conditions of,the agreement, Horizon ... At its option, Pharmasset may receive a second loan ... loan of $10 million by November 30, 2008,provided conditions ...
... Inc.,(Nasdaq: COHR ) today announced that, as anticipated, ... Determination letter indicating that,the Company is not currently in ... Rules 4350(e) and 4350(g) due to,its failure to hold ... September 30, 2007. The notice stated that the failure ...
Cached Biology Technology:Pharmasset Accesses up to $30 Million of Working Capital 2Pharmasset Accesses up to $30 Million of Working Capital 3Coherent, Inc. Receives Expected Letter from NASDAQ 2
(Date:9/18/2014)... September 18, 2014 Elsevier, a world-leading provider ... services, and the Australasian Research Management Society (ARMS), ... scientists at the 5th Scopus Young Researcher of ... earlier today at the National Convention Centre and ... was attended by guests representing Australia,s and New ...
(Date:9/18/2014)... roaches of the fish world, are the ideal animal ... shape. They,ve moved from the ocean into tens of ... each time changing their skeleton to adapt to the ... populations of sticklebacks now have turned up one of ... a simple change in that gene,s regulation in a ...
(Date:9/18/2014)... found clownfish larvae can swim up to 400 kilometres in ... cope with environmental change. , Clownfish spend their entire adult ... babies they must wander the open ocean, says study co-author, ... Coral Reef Studies (Coral CoE) at James Cook University. , ... now we,ve been given a rare glimpse into how far ...
Breaking Biology News(10 mins):Elsevier and Australasian Research Management Society award scientists at Scopus ceremony 2Counting fish teeth reveals regulatory DNA changes behind rapid evolution, adaptation 2Counting fish teeth reveals regulatory DNA changes behind rapid evolution, adaptation 3Nemo's epic journey to find a new home 2
... Hoboken, N.J.February 19, 2013John Wiley & Sons, Inc., announced today ... Microbial Biotechnology have joined the Wiley Open Access ... open access and free to view, download and share. ... Molecular Medicine has attracted very high quality submissions and ...
... was only something that was necessary to keep your car ... and animals living in cold climates have natural antifreeze proteins ... matter. Without such antifreeze, living matter would suffer from frost ... proteins is one of the major evolutionary routes taken by ...
... Whales are mammals, but they don,t look like the mammals ... tail, no hind legs and no body hair. And inside ... simpler and ,peg like,. A multidisciplinary team of researchers have ... process to investigate how the whale got its teeth. ...
Cached Biology News:Established journals to publish under open access model 2Not just cars, but living organisms need antifreeze to survive 2Not just cars, but living organisms need antifreeze to survive 3How the whale got its teeth 2How the whale got its teeth 3
PEG 8000, Molecular Biology Grade...
...
... (HDAC) Antibody Sampler Kit Kit Includes: ... #2062, 40 ul Histone Deacetylase 3 (HDAC3) ... 4 (HDAC4) Antibody, Catalog #2072, 40 ul ... 40 ul Histone Deacetylase 6 (HDAC6) Antibody, ...
Recombinant Mouse Matrilin-4, CF Protein Family: Extracellular Matrix Molecules, Chondrogenesis Markers...
Biology Products: