Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

based workflow. This user-friendly and unlabeled approach to relative quantitation of MS data will have wide implications on the LC-MS based proteomics workflow for differential analysis.


Acknowledgment
Peter Brostedt and Ingrid Holmquist, Pharmacia Diagnostics AB, Uppsala, Sweden, are acknowledged for generously supplying extracted proteins from olive pollen.


References
1. Huecas, S. et al. J. Biol. Chem. 276, 2795927966 (2001).

2. Application note: Automated identification and quantitation of proteins and peptides using LC-MS/MS, GE Healthcare, 11-0027-39, Edition AA (2005).

3. Application note: Reproducible and robust peptide separations using Ettan MDLC, a multidimensional liquid chromatography system, GE Healthcare, 11-0031-13, Edition AA (2005).



back to top
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:10/22/2014)... Rock Hill, SC (PRWEB) October 22, 2014 ... the expansion of its cardiovascular pharmacogenetics menu, which ... improved patient outcomes. With PCLS’s evidence-based results, ... individual needs and optimize their therapy, while minimizing ... , In the U.S., according to the FDA ...
(Date:10/22/2014)... 22, 2014 Nuvilex, Inc. (OTCQB: NVLX) – ... people worldwide are living with diabetes, with  that number ... The global market for diabetes treatments is approximately $500 ... worldwide died from pancreatic cancer.  Pancreatic cancer is the ... in the United States , and ...
(Date:10/20/2014)... Mapp Biopharmaceutical,s valiant effort to produce ... fight the Ebola outbreak will make the public ... of pharmaceuticals can be, according to Kalorama Information.  ... some may be taken aback by the three-month ... knowledge are well aware of the complex issues ...
(Date:10/20/2014)... , October 20, 2014 , ... (GI) disorders and the delivery of care has revealed ... and worrying inequalities in the provision of healthcare services ... was commissioned by United European Gastroenterology (UEG), have been ... and public awareness of the burden of GI disorders ...
Breaking Biology Technology:PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3Nuvilex Brief Analyst Report: Thinking Outside the Box by BrokerBank Securities, Inc. 2Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 2Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 3Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 2Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 3Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 4Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 5
... Milwaukee, Wis. - In a move designed ... Gov. Jim Doyle has announced a partnership agreement with ... companies that conduct stem cell research at non-profit and ... under the stem cell patents held by WARF. , ...
... Wis. - The Center for International Blood and ... Wisconsin has won a contract worth $6,180,029 to collect ... cell transplants that use cells from bone marrow or cord ... part of the U.S. Department of Health and Human ...
... This week, the clock starts ticking on the 18-month window ... on the city's wireless Internet service. , ,After months of ... EarthLink and Cellnet , Mayor Tom Barrett ... access to city facilities to build a $20 million "Wi-Fi" ...
Cached Biology Technology:Doyle, WARF announce partnership to lure stem cell companies 2Doyle, WARF announce partnership to lure stem cell companies 3Medical College to build $6M transplant database 2Milwaukee could be wireless in 18 months 2
(Date:10/16/2014)... October 16, 2014 NXT-ID, Inc. (NASDAQ: ... a biometric authentication company focused on the growing mobile ... technologies including Wocket™, the Smart Wallet, at the 13 ... is a leading global conference on the intersection of ... Mark Anderson , founder and publisher of the ...
(Date:10/15/2014)... risk from strains of influenza virus increases with the ... become complacent that the most substantial threats have been ... pandemics arise when a new virus strain – against ... spreads in the human population. There have been five ... of which – the 1918 Spanish Flu – cost ...
(Date:10/15/2014)... Johannes Gutenberg University Mainz (JGU) as a full ... to maintain a significant nationwide standing in the ... of Johannes Gutenberg University Mainz as a full ... the national and international competition for the best ... can be proud that Mainz University has been ...
Breaking Biology News(10 mins):NXT-ID Inc. to Present at 13th Annual FiRe Conference 2NXT-ID Inc. to Present at 13th Annual FiRe Conference 3Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 2Johannes Gutenberg University Mainz joins Germany's Gauss-Allianz as a full member 3
... Calif., May 9, 2012 Human cells are thought to ... that help determine which genes are turned on or off ... normal cellular function, but they can also contribute to human ... promote cell survival. But to better understand how miRNAs influence ...
... to understand how the aging process affects the brain ... older adults. "Everybody ages differently. By looking at ... health, we begin to understand that preventable factors may ... University psychology doctoral student Andrew Bender, lead author of ...
... May 9, 2012 (Bronx, NY) Researchers at ... University have discovered a molecule that inhibits the action ... in the growth, maintenance and repair of reproductive tissues ... The molecule, discovered in animal studies, could lead to ...
Cached Biology News:It's a trap! New laboratory technique captures microRNA targets 2WSU researchers say genes and vascular risk modify effects of aging on brain and cognition 2WSU researchers say genes and vascular risk modify effects of aging on brain and cognition 3Molecule found that inhibits estrogen, key risk factor for endometrial and breast cancers 2
...
... This CLS number is a ... match Cornings product number. If showing ... the old Sigma-Aldrich number (R4276) or ... Material: cap polystyrene (expanded surface, easy ...
... Custom Peptide Services offer a full range ... research needs. Services include: Peptide synthesis, ... Peptide synthesis, purified product (70-95% pure) ... amidation, biotinylation, fluorescein, myristylation, phosphorylation, and rhodamine) ...
... ELISPOT (enzyme-lined immunospot) Kit analyzes interferon gamma ... A spot forms at the site where ... using a dissection microscope or automated ELISPOT ... of cells added to the microplate. This ...
Biology Products: