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Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

based workflow. This user-friendly and unlabeled approach to relative quantitation of MS data will have wide implications on the LC-MS based proteomics workflow for differential analysis.


Acknowledgment
Peter Brostedt and Ingrid Holmquist, Pharmacia Diagnostics AB, Uppsala, Sweden, are acknowledged for generously supplying extracted proteins from olive pollen.


References
1. Huecas, S. et al. J. Biol. Chem. 276, 2795927966 (2001).

2. Application note: Automated identification and quantitation of proteins and peptides using LC-MS/MS, GE Healthcare, 11-0027-39, Edition AA (2005).

3. Application note: Reproducible and robust peptide separations using Ettan MDLC, a multidimensional liquid chromatography system, GE Healthcare, 11-0031-13, Edition AA (2005).



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