Navigation Links
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

be identified and quantitated by using DeCyder MS.

The graph extracted from the PepMatch module in Figure 6 shows the sequences and signal intensities of the significantly varying peptides that were used for quantitation of different isoforms of Ole e 1. As earlier mentioned, LGMYPPNM is present in all forms of Ole e 1, resulting in the highest signal intensity or most blackened grayscale in the intensity maps created by DeCyder MS. The other peptide pairs in Figure 6 thus represent unique peptides for five different isoforms of the olive allergen.

In Table 1, a list of significantly varying peptides from the two allergen samples is shown, together with Student t-test p values, protein names, pI values, molecular masses, and accession numbers. As can be seen, several of the isoforms were very close in molecular mass and pI value. The smallest deviation in mass was 64 Da between Ole 1 c (16485 Da) and Ole e 1.0103 (16421 Da), while the smallest deviation in pI value was 0.02 units between Ole e 1 (CAA73036.1) and Ole e 1.0103 (CAA73037.1).


Conclusions
DeCyder MS Differential Analysis Software is a tool for the visualization and label-free relative quantitation of complex protein digests. The precise gradient formation and solvent delivery features of Ettan MDLC provided highly reproducible retention times for eluted peptides, resulting in improved matching performance by DeCyder MS.

Quantitation of allergen samples using DeCyder MS revealed a 3.8-fold difference in Ole e 1 content, which is in agreement with other physicochemical quantitation methods. In addition, five differentially regulated isoforms of Ole e 1 could be unambiguously identified and quantitated.

The results indicate that for quantitation of proteins containing microheterogeneities, the LC-MS based proteomics workflow combined with DeCyder MS evaluation could be preferable to the gel-
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Comparison of different protein quantitation methods
2. Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
3. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
4. Outstanding transfection rates using the Multiporator
5. Outstanding transfection rates using the Multiporator
6. Isolation of Cosmids using Eppendorfs Perfectprep Plasmid Mini Kit
7. Normalizing cDNA libraries using the EppendorfThermomixer
8. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer
11. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
Post Your Comments:
(Date:7/22/2014)... 22, 2014 The Board of Directors of BD ... declared a quarterly dividend of 54.5 cents per common share ... September 9, 2014. The indicated annual dividend rate is $2.18 ... is a leading medical technology company that partners with customers ... and evolving health needs. Our innovative solutions are focused on ...
(Date:7/22/2014)... Even within a phylum so full of mean little ... arthropods for its cruelty -- at least to crickets. Native ... is a most predatory sort of parasite. It swoops onto ... of larvae, and leaves its wicked brood to invade, kill ... of this would be possible without the fly,s ability to ...
(Date:7/22/2014)... Researchers from the National Institute of Standards and ... have demonstrated a new design for an atomic ... comb, or a microcomb. , The microcomb clock, ... of the new journal Optica ,* is ... microcomb, and its accurate conversion of optical frequencies ...
(Date:7/22/2014)... metal nanoparticles in water with ultrasound and they,ll spin ... No one yet knows exactly. But researchers at the ... their speedand it,s fast. At up to 150,000 revolutions ... any nanoscale object submerged in liquid ever reported. , ... the possibility that they could be used not only ...
Breaking Biology Technology:Fly-inspired sound detector 2Fly-inspired sound detector 3Fly-inspired sound detector 4'Comb on a chip' powers new NIST/Caltech atomic clock design 2NIST shows ultrasonically propelled nanorods spin dizzyingly fast 2
... , ... goBalto.com introduces a new project management tool - "Projects" - to help ... management tools can be awkward and cumbersome. goBalto Projects takes on project management ... time and money. , ...
... , PHOENIX and SAN CARLOS, Calif., Oct. 10 ... data today from its Phase 2 clinical trial of ... (AAPM) 20th Annual Clinical Meeting in Phoenix, Arizona. ... stage investigational product candidate in clinical development for the ...
... of Toronto have developed a new "lab-on-a-chip" technique that analyses ... at risk of breast cancer much more quickly than ever ... metabolites the products of metabolized estrogen in breast ... patients compared to healthy women, and is therefore believed to ...
Cached Biology Technology:goBalto Introduces Web-based Solution to Manage Drug-development Collaborations 2Topline Results from Phase 2 Clinical Trial of Oral NKTR-118 Presented at 20th American Academy of Pain Management Annual Clinical Meeting (AAPM) in Phoenix 2Topline Results from Phase 2 Clinical Trial of Oral NKTR-118 Presented at 20th American Academy of Pain Management Annual Clinical Meeting (AAPM) in Phoenix 3Topline Results from Phase 2 Clinical Trial of Oral NKTR-118 Presented at 20th American Academy of Pain Management Annual Clinical Meeting (AAPM) in Phoenix 4New lab-on-a-chip technique developed at U of T 2
(Date:7/22/2014)... of planthopper known as Conosimus, which now includes six ... the southern part of the Iberian Peninsula in the ... in the open-access Journal of Insect Science ... Conosimus baenai , has been named after Manuel Baena, ... of Iberian Hemiptera. , Conosimus baenai differs ...
(Date:7/21/2014)... a professor of entomology and cooperating faculty ... the University of California, Riverside, has been awarded the ... of Chemical Ecology ( ISCE ). , Awarded first ... the society,s highest honor. It is awarded annually to ... the study of chemicals that mediate interactions between living ...
(Date:7/21/2014)... to monitor seals, every movement, researchers have shown for ... to offshore wind farms and pipelines. Those man-made structures ... according to a study published in the Cell Press ... , "I was shocked when I first saw the ... Shoal," an offshore wind farm in the United Kingdom, ...
Breaking Biology News(10 mins):UC Riverside entomologist receives international honor for chemical ecology contributions 2Seals forage at offshore wind farms 2
... the egg genome? Researchers have answered a similarly vexing ... of long stretches of repeated DNA in the human genome ... published online by Nature Genetics on October 7, ... the human genome that are partly responsible for both disease ...
... CITY, Utah - Mario R. Capecchi, Ph.D., distinguished professor ... Utahs Eccles Institute of Human Genetics and a Howard ... Prize in Physiology or Medicine. The announcement was ... Karolinska Institute, in Stockholm, Sweden. The prize recognizes Capecchis ...
... of Cincinnati (UC) radiologists have developed a new ... eases diagnosis of venous diseases. Multi-detector computed ... three-dimensional images of arteries, the vessels which carry ... blood throughout the body. Veins, smaller vessels that ...
Cached Biology News:Which came first, the chicken genome or the egg genome? 2Which came first, the chicken genome or the egg genome? 3Gene-targeting pioneer Mario Capecchi shares 2007 Nobel Prize in Medicine 2Gene-targeting pioneer Mario Capecchi shares 2007 Nobel Prize in Medicine 3New thoracic imaging approach can pinpoint underlying venous problems 2
DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides. Ribonuclease activity has been reduced to non-detectable levels....
... coli containing a clone of the human Topoisomerase ... II alters the topological state of nucleic acids ... transient break which generates a separate DNA helix ... DNA passage mechanism, the enzyme can relax negatively ...
RNase-Free DNase 50 l...
supplied with 10x reaction buffer...
Biology Products: